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The map label for this gene is alaS [H]

Identifier: 197294554

GI number: 197294554

Start: 500619

End: 503210

Strand: Reverse

Name: alaS [H]

Synonym: PAa_0509

Alternate gene names: 197294554

Gene position: 503210-500619 (Counterclockwise)

Preceding gene: 197294555

Following gene: 197294553

Centisome position: 57.19

GC content: 28.32

Gene sequence:

>2592_bases
ATGAAATCTTTTGCAATTAGAAAAATGTGGCTTGATTTTTTTTCTAGCAAAGGTCATCATATAGAAAAATCATTTTCTTT
AATTCCTCATCAAGATCCTACTTTATTATGGGTTAACGCAGGTGTTACACCTTTAAAAAAATACTTCGATGGTAGCCAAA
CCCCACTTTGCAAAAAAATTGTCAATGTTCAAAAATGCATTCGCACTAATGATATCGAAAATGTTGGAGAAACTGCACGC
CATCATACTTTTTTTGAAATGTTAGGCAATTTTTCAATCGGTGATTATTTTAAAAAAGAAGCCATCCATTACGCCTATGA
ATTATTAACCTCTTCCAAATGGTTTTCCTTTCCTTTGGATAAATTATATATCACCTATTATCCCACAGACAAAGATACAT
ATAAATATTGGTTAGAATTAGGGATACGAAAAGATAGACTTATTCCTTTAAAAAGTAATTTTTGGCAAATTGGTCCAGGC
CCTTCTGGTCCTTGTACAGAAATTTTTTTCGATAGAGGCGAAAAATATGACAAACGCAATAAAGAATTAATTATTGAAGA
TTTAGAAAATAATCGTTTTATTGAAATTTGGAATATTGTTTTTTCTCAATATAATTGCGATCCGAAACTTTCTATCGAAC
AATACCAAGAACTTCCAAATAAAAATATTGATACAGGGGCAGGCCTTGAACGTTTGGCAGCAATTCTGCAAGAAGCTGAT
AATAATTTTGAAACTGATCTTTTTTTTCCTTTAATTAAATCAATAGCAAAACTATCTCAAATCCCTTACCACCAAAGCCC
TAAAGCTTTTAAAATAATTGCAGATCACTTAAAAACACTTGTTTTTGCCATTAATGATGGAGCGACTTTGACCAACGAAA
AAAGAGGTTATGTTTTAAAAAAACTTTTAAGAAGATCATTCATTCAAGGAAAAAAACTTAATTTTAATCAACCTTTTTTA
CATAATTTAGTGGATCCAACAATTAATATGATGCAAGATTTTTATCCTGAATTAACTAAAAATAAAAATTATATTAAAGA
TATTTTGCTTAAGCAAGAACATGTTTTTTTAGAAACTTTAAAAACTGCAGAAGCTAAATTTCTTTATTATGCTAAGAAAA
AAAGTTTATCAGGAATAAATTTTTTTAAATTATATGATACTTACGGTATTCCTGAAGATGTAATTATAGATTATGCTAAA
ATCAACAATGTTTCTGTTGATTACAAGGGATTTAAATTCTTATTGGAAAAACACCGACAAATTTCACAAAAAAATCATAT
TTTTATTTCCAAAATGAATCAGCAAGAAGAAAAATTTTTAAATTTTCGTGAAAAAAGTGAATTTTTAGGTTATAATAATT
TTGCTCTTAAAACTAAAGTAATTAAGGTTTTTGATAAAGGAATAGTTTTGGCGCAAACCCCATTTTATGCACCTATGGGA
GGACAAATAGCTGATGAAGGTTTTATTGATGGAAAAAAAGTAAAAAATATTACTAAATTACCAAACGGGCAACATTTGCA
CCAAATAGAAGGATCTTTTTATGAAGGACAAGAGGTTTTTGCTCAAATAGATAAAAAAGTAAGGGATCAAATTTCTTATC
ATCATACAGCAACACATCTTTTAGAAACTGTTTTACAAAATGAACTAGGAAGTCACTTAGAAAAACAAGGTTCATCGGTT
GGATATAATTCTTTGCGTTATGATTTCAATCACTTTGAAAAATTAACCCCCGAAAAATTAATTAAAATCGAAAAAGAAAT
CAACGGTTTAATTAAAAAAAATTTATCTGTTGAAATCCAAAAGATTCCAACACAAGAAGCGAAAGAAAATTATAGCGATA
TTTTGAGCAAAAATCCTAAAGCAAAATACAAAGACGAAGTGAGAATAGTAAGAATAGGGAATGATTTTTCAATTGATTTA
TGTGGCGGAACTCATGCTTCAGAAACTAAAAATTTAGAAAAATTCGTTATCTTATCTTATGAAACCATCAGCTCAGGAAT
TTATCGAATCGAAGCAGTTTGCGGCAATAACATCAAAACTGCTTTAGTTGATAAATTAAAACCTTATCAATTGACAGTAA
AACAATTGATACAAAAAGCACAAACAAAAAAGCTCTTTTTTGAAGCTCTTCCTGTTCCACAAATTAATCAAAGTTATCAA
GATATTCTTGATTATCAAAACTATCTCAAAGAATTACAAAAACAAATATTTATTTGTGAAAAAAAAGACTTAGAGTATCG
TTGCAAAAAAATGATTCAAGAAAAAGATAAGTTTTTGCCTGAAAAAATTTCCAAAGAAACGTTAATTATCATTGAAGAAG
ATATAAACATGGAAGTTTTAAAATGTTTTATGGATAATATTTTTGATACTTTAAAAATTGACATTTTATTTATTTGCCAA
TTAAATCTTTCTAAAGTTGTTTTCTTGTGCAAAAGCAAAACTTGGCATGCAGGAAAATTAGTGAAAGAAATTTCTTCATT
AGCTTTAGGTTCTGGCGGCGGAAATGCTTTTTTAGCGCAAGGAGGTTCCAAAACAACTCAAACCATCAAGCAAACAATTA
AAACCTTAAAATATAAATTTAAAGTTGCTTAG

Upstream 100 bases:

>100_bases
GAAAAGTTATATAAAAAAAGTTAAAGTAATTATGGTTTGTTTTTTTATAATAAATGTCTAAAATACAATCATTCAAAATT
AAAAAGGAGTTAATAAATTC

Downstream 100 bases:

>100_bases
AGAAGGAGTTTTTTATTATTAATGTAAATCATTGTAATTCTTATTTGGGTCTGGATTTAGGCGAAAAAACCCTGGGAATC
GCACTATCCGAAACAGGAAT

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 863; Mature: 863

Protein sequence:

>863_residues
MKSFAIRKMWLDFFSSKGHHIEKSFSLIPHQDPTLLWVNAGVTPLKKYFDGSQTPLCKKIVNVQKCIRTNDIENVGETAR
HHTFFEMLGNFSIGDYFKKEAIHYAYELLTSSKWFSFPLDKLYITYYPTDKDTYKYWLELGIRKDRLIPLKSNFWQIGPG
PSGPCTEIFFDRGEKYDKRNKELIIEDLENNRFIEIWNIVFSQYNCDPKLSIEQYQELPNKNIDTGAGLERLAAILQEAD
NNFETDLFFPLIKSIAKLSQIPYHQSPKAFKIIADHLKTLVFAINDGATLTNEKRGYVLKKLLRRSFIQGKKLNFNQPFL
HNLVDPTINMMQDFYPELTKNKNYIKDILLKQEHVFLETLKTAEAKFLYYAKKKSLSGINFFKLYDTYGIPEDVIIDYAK
INNVSVDYKGFKFLLEKHRQISQKNHIFISKMNQQEEKFLNFREKSEFLGYNNFALKTKVIKVFDKGIVLAQTPFYAPMG
GQIADEGFIDGKKVKNITKLPNGQHLHQIEGSFYEGQEVFAQIDKKVRDQISYHHTATHLLETVLQNELGSHLEKQGSSV
GYNSLRYDFNHFEKLTPEKLIKIEKEINGLIKKNLSVEIQKIPTQEAKENYSDILSKNPKAKYKDEVRIVRIGNDFSIDL
CGGTHASETKNLEKFVILSYETISSGIYRIEAVCGNNIKTALVDKLKPYQLTVKQLIQKAQTKKLFFEALPVPQINQSYQ
DILDYQNYLKELQKQIFICEKKDLEYRCKKMIQEKDKFLPEKISKETLIIIEEDINMEVLKCFMDNIFDTLKIDILFICQ
LNLSKVVFLCKSKTWHAGKLVKEISSLALGSGGGNAFLAQGGSKTTQTIKQTIKTLKYKFKVA

Sequences:

>Translated_863_residues
MKSFAIRKMWLDFFSSKGHHIEKSFSLIPHQDPTLLWVNAGVTPLKKYFDGSQTPLCKKIVNVQKCIRTNDIENVGETAR
HHTFFEMLGNFSIGDYFKKEAIHYAYELLTSSKWFSFPLDKLYITYYPTDKDTYKYWLELGIRKDRLIPLKSNFWQIGPG
PSGPCTEIFFDRGEKYDKRNKELIIEDLENNRFIEIWNIVFSQYNCDPKLSIEQYQELPNKNIDTGAGLERLAAILQEAD
NNFETDLFFPLIKSIAKLSQIPYHQSPKAFKIIADHLKTLVFAINDGATLTNEKRGYVLKKLLRRSFIQGKKLNFNQPFL
HNLVDPTINMMQDFYPELTKNKNYIKDILLKQEHVFLETLKTAEAKFLYYAKKKSLSGINFFKLYDTYGIPEDVIIDYAK
INNVSVDYKGFKFLLEKHRQISQKNHIFISKMNQQEEKFLNFREKSEFLGYNNFALKTKVIKVFDKGIVLAQTPFYAPMG
GQIADEGFIDGKKVKNITKLPNGQHLHQIEGSFYEGQEVFAQIDKKVRDQISYHHTATHLLETVLQNELGSHLEKQGSSV
GYNSLRYDFNHFEKLTPEKLIKIEKEINGLIKKNLSVEIQKIPTQEAKENYSDILSKNPKAKYKDEVRIVRIGNDFSIDL
CGGTHASETKNLEKFVILSYETISSGIYRIEAVCGNNIKTALVDKLKPYQLTVKQLIQKAQTKKLFFEALPVPQINQSYQ
DILDYQNYLKELQKQIFICEKKDLEYRCKKMIQEKDKFLPEKISKETLIIIEEDINMEVLKCFMDNIFDTLKIDILFICQ
LNLSKVVFLCKSKTWHAGKLVKEISSLALGSGGGNAFLAQGGSKTTQTIKQTIKTLKYKFKVA
>Mature_863_residues
MKSFAIRKMWLDFFSSKGHHIEKSFSLIPHQDPTLLWVNAGVTPLKKYFDGSQTPLCKKIVNVQKCIRTNDIENVGETAR
HHTFFEMLGNFSIGDYFKKEAIHYAYELLTSSKWFSFPLDKLYITYYPTDKDTYKYWLELGIRKDRLIPLKSNFWQIGPG
PSGPCTEIFFDRGEKYDKRNKELIIEDLENNRFIEIWNIVFSQYNCDPKLSIEQYQELPNKNIDTGAGLERLAAILQEAD
NNFETDLFFPLIKSIAKLSQIPYHQSPKAFKIIADHLKTLVFAINDGATLTNEKRGYVLKKLLRRSFIQGKKLNFNQPFL
HNLVDPTINMMQDFYPELTKNKNYIKDILLKQEHVFLETLKTAEAKFLYYAKKKSLSGINFFKLYDTYGIPEDVIIDYAK
INNVSVDYKGFKFLLEKHRQISQKNHIFISKMNQQEEKFLNFREKSEFLGYNNFALKTKVIKVFDKGIVLAQTPFYAPMG
GQIADEGFIDGKKVKNITKLPNGQHLHQIEGSFYEGQEVFAQIDKKVRDQISYHHTATHLLETVLQNELGSHLEKQGSSV
GYNSLRYDFNHFEKLTPEKLIKIEKEINGLIKKNLSVEIQKIPTQEAKENYSDILSKNPKAKYKDEVRIVRIGNDFSIDL
CGGTHASETKNLEKFVILSYETISSGIYRIEAVCGNNIKTALVDKLKPYQLTVKQLIQKAQTKKLFFEALPVPQINQSYQ
DILDYQNYLKELQKQIFICEKKDLEYRCKKMIQEKDKFLPEKISKETLIIIEEDINMEVLKCFMDNIFDTLKIDILFICQ
LNLSKVVFLCKSKTWHAGKLVKEISSLALGSGGGNAFLAQGGSKTTQTIKQTIKTLKYKFKVA

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=971, Percent_Identity=31.6168898043254, Blast_Score=384, Evalue=1e-106,
Organism=Homo sapiens, GI38569417, Length=958, Percent_Identity=26.6179540709812, Blast_Score=308, Evalue=2e-83,
Organism=Escherichia coli, GI1789048, Length=883, Percent_Identity=35.3340883352208, Blast_Score=511, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI17506981, Length=983, Percent_Identity=28.1790437436419, Blast_Score=348, Evalue=8e-96,
Organism=Caenorhabditis elegans, GI17536681, Length=750, Percent_Identity=31.2, Blast_Score=319, Evalue=4e-87,
Organism=Saccharomyces cerevisiae, GI6324911, Length=756, Percent_Identity=35.5820105820106, Blast_Score=406, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24582809, Length=968, Percent_Identity=30.0619834710744, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI45552267, Length=968, Percent_Identity=30.0619834710744, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24658214, Length=810, Percent_Identity=27.037037037037, Blast_Score=261, Evalue=1e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 100058; Mature: 100058

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSFAIRKMWLDFFSSKGHHIEKSFSLIPHQDPTLLWVNAGVTPLKKYFDGSQTPLCKKI
CCCHHHHHHHHHHHCCCCCEEHHHCCCCCCCCCCEEEEECCCHHHHHHCCCCCCHHHHHH
VNVQKCIRTNDIENVGETARHHTFFEMLGNFSIGDYFKKEAIHYAYELLTSSKWFSFPLD
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
KLYITYYPTDKDTYKYWLELGIRKDRLIPLKSNFWQIGPGPSGPCTEIFFDRGEKYDKRN
EEEEEEECCCCHHHHHHHHHCCCCCCEEEECCCCEECCCCCCCCHHHHHHHCCCCCCCCC
KELIIEDLENNRFIEIWNIVFSQYNCDPKLSIEQYQELPNKNIDTGAGLERLAAILQEAD
CCEEEEECCCCCEEEHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHC
NNFETDLFFPLIKSIAKLSQIPYHQSPKAFKIIADHLKTLVFAINDGATLTNEKRGYVLK
CCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHH
KLLRRSFIQGKKLNFNQPFLHNLVDPTINMMQDFYPELTKNKNYIKDILLKQEHVFLETL
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
KTAEAKFLYYAKKKSLSGINFFKLYDTYGIPEDVIIDYAKINNVSVDYKGFKFLLEKHRQ
HHHHHHHEEEEHHCCCCCCEEEEEECCCCCCHHHEEEHHHCCCCEECHHHHHHHHHHHHH
ISQKNHIFISKMNQQEEKFLNFREKSEFLGYNNFALKTKVIKVFDKGIVLAQTPFYAPMG
HHHCCCEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCC
GQIADEGFIDGKKVKNITKLPNGQHLHQIEGSFYEGQEVFAQIDKKVRDQISYHHTATHL
CCCCCCCCCCCHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LETVLQNELGSHLEKQGSSVGYNSLRYDFNHFEKLTPEKLIKIEKEINGLIKKNLSVEIQ
HHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEE
KIPTQEAKENYSDILSKNPKAKYKDEVRIVRIGNDFSIDLCGGTHASETKNLEKFVILSY
ECCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCEEEEEEH
ETISSGIYRIEAVCGNNIKTALVDKLKPYQLTVKQLIQKAQTKKLFFEALPVPQINQSYQ
HHHHCCCEEEEEHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
DILDYQNYLKELQKQIFICEKKDLEYRCKKMIQEKDKFLPEKISKETLIIIEEDINMEVL
HHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHCCHHHCCCCEEEEEECCCCHHHH
KCFMDNIFDTLKIDILFICQLNLSKVVFLCKSKTWHAGKLVKEISSLALGSGGGNAFLAQ
HHHHHHHHHHHHEEEEEEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEC
GGSKTTQTIKQTIKTLKYKFKVA
CCCHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure
MKSFAIRKMWLDFFSSKGHHIEKSFSLIPHQDPTLLWVNAGVTPLKKYFDGSQTPLCKKI
CCCHHHHHHHHHHHCCCCCEEHHHCCCCCCCCCCEEEEECCCHHHHHHCCCCCCHHHHHH
VNVQKCIRTNDIENVGETARHHTFFEMLGNFSIGDYFKKEAIHYAYELLTSSKWFSFPLD
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
KLYITYYPTDKDTYKYWLELGIRKDRLIPLKSNFWQIGPGPSGPCTEIFFDRGEKYDKRN
EEEEEEECCCCHHHHHHHHHCCCCCCEEEECCCCEECCCCCCCCHHHHHHHCCCCCCCCC
KELIIEDLENNRFIEIWNIVFSQYNCDPKLSIEQYQELPNKNIDTGAGLERLAAILQEAD
CCEEEEECCCCCEEEHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHC
NNFETDLFFPLIKSIAKLSQIPYHQSPKAFKIIADHLKTLVFAINDGATLTNEKRGYVLK
CCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHH
KLLRRSFIQGKKLNFNQPFLHNLVDPTINMMQDFYPELTKNKNYIKDILLKQEHVFLETL
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
KTAEAKFLYYAKKKSLSGINFFKLYDTYGIPEDVIIDYAKINNVSVDYKGFKFLLEKHRQ
HHHHHHHEEEEHHCCCCCCEEEEEECCCCCCHHHEEEHHHCCCCEECHHHHHHHHHHHHH
ISQKNHIFISKMNQQEEKFLNFREKSEFLGYNNFALKTKVIKVFDKGIVLAQTPFYAPMG
HHHCCCEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCC
GQIADEGFIDGKKVKNITKLPNGQHLHQIEGSFYEGQEVFAQIDKKVRDQISYHHTATHL
CCCCCCCCCCCHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LETVLQNELGSHLEKQGSSVGYNSLRYDFNHFEKLTPEKLIKIEKEINGLIKKNLSVEIQ
HHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEE
KIPTQEAKENYSDILSKNPKAKYKDEVRIVRIGNDFSIDLCGGTHASETKNLEKFVILSY
ECCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCEEEEEEH
ETISSGIYRIEAVCGNNIKTALVDKLKPYQLTVKQLIQKAQTKKLFFEALPVPQINQSYQ
HHHHCCCEEEEEHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
DILDYQNYLKELQKQIFICEKKDLEYRCKKMIQEKDKFLPEKISKETLIIIEEDINMEVL
HHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHCCHHHCCCCEEEEEECCCCHHHH
KCFMDNIFDTLKIDILFICQLNLSKVVFLCKSKTWHAGKLVKEISSLALGSGGGNAFLAQ
HHHHHHHHHHHHEEEEEEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEC
GGSKTTQTIKQTIKTLKYKFKVA
CCCHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA