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The map label for this gene is ltrA [H]

Identifier: 197294521

GI number: 197294521

Start: 467077

End: 468642

Strand: Reverse

Name: ltrA [H]

Synonym: PAa_0471

Alternate gene names: 197294521

Gene position: 468642-467077 (Counterclockwise)

Preceding gene: 197294522

Following gene: 197294520

Centisome position: 53.26

GC content: 36.33

Gene sequence:

>1566_bases
ATGCTTACACATAATTCAAATGAATTTAAGCTTTCGAGAACTTTAGATATGATTTTAAATCATTCAAAAAACAACTATAG
CCTAAAAAAGGTATTGAAACGCGAAATGAATAACATAGAAAACACATACAAAGCATTCAATAGCATTGCAACCAATAAAG
GCAGCGGTACAGAGGGCGTTGGCAACAAAACCATTGACGGCATAAAGCTAGAAATGATAAAAAAATACCACAAAGAATAC
GTCAACAACCAATACAATCCGCAACCTGTCAAAAAAGTATTAATACCAAAAGGTAAAAATAAAACGAGACCGCTAGGCAT
CCCAACCATAAAAGATAGAATAATCCAGAAAGCAATGGAACAACTTTTAACCCTCTATTTTGAAAACATCTTCTTAGAAT
GGAGCTTCGGATTCAGAAGCAAGAAATCATGCCATGATGCCGTAAAACGCGTCAAACAAAGATTCAAAGGGATAGATTAT
ATAATAAAAATCGACATTAAAGGTTACTTTGAAACCATCAACCACGATATCCTCAATAAGATGTTAAACAAATACATCAG
AAAGGCCAAAACGTTAAAAACAATCAACCAATGGCTCAAAGCAGGAATAATGGAAAATGGAATCAAATACGAATCATTAT
CAGGAACACCGCAAGGTGGGATAATATCCCCTTTATTATCAAATATATATTTACATTACATCGATAAGAAAATGGAAGAA
CTGATAAGAAACGGCAAACCGATAATGAAAGCAAATCCCGAATATTGGAAGGCATACACAAAAAAACAACATCACAATCT
ATCAATAGATAGTGAAATAAACCTGAACCCAAACCCGAGAATAGAATACATCCGCTACGCAGATGATTTTATTATTGGAA
TAAAAGGAGAACACCACGAAGCAGAAAGGATTAAAACCCATGTACTCAAATGGCTAGAACAAGACTTGAAACTCGTGGTA
AACCGCGACAAATCAAAAATTGTAAAAACCACTAAGGGCACAAGGTTCCTATCTTATATGGTAAAGGTAAACCCAACCAA
CAAAACTCGCAAAAAGAAAACAACAAAAAACTCACTGAACGGGCAAGTACAAATCCAAATCCCAAAAGACACAACAAGAG
ACTACGGGAAAGAATACGGTTGGACCAAAAACGGGAAAGTAAAACACGACGAAACACTAGCAAGTAGGGAAGCACTAGAA
ATAATACGAACCTACAAATCAATAACGCTCGGCATTATCCGATACTTTTGTCTAGGAAACAACCTAGCACAATTAACACA
TTTATACTATTTGGCGGAATACAGTTGTTTAAAAACACTAGTGAGAAAATGGAAAACATCTATCGCAAAAGTTCGGCGAA
AATTAAGGAACGGCAAAACTTGGGGGATTCAATACAACCTCAAGAATAAAACCAAAGTTGATAGCTGGGACAAACTGGAC
TGGGAAAAGATAAAAAGGATGAGAAAAACAAAGGGGAATCCCGATGTAATTATCAACTGTTATCTCTACCAAGGACGAAC
GGCCTTGGTCGCGGACAGCACCGTGAATACACCCCAACTGACCTAG

Upstream 100 bases:

>100_bases
ATTGCCCTCCCAGGGCTCTTAGGAAAACTAAGAACAAGGCGAAAACAAGTCAGTCATTTATGGTATATCTAAACAAATCA
AGAAAGGAGTAAGTGATAAT

Downstream 100 bases:

>100_bases
CGCCTTTTGTTAAAAACCCTTTTGACAAATGGAAAATAGAAAACAACATTATTGATTATTTTGATACTTTCCATATGACT
CATAAAGGTGATGATTCATA

Product: Retron-type reverse transcriptase

Products: NA

Alternate protein names: Reverse-transcriptase; RNA maturase; DNA endonuclease [H]

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MLTHNSNEFKLSRTLDMILNHSKNNYSLKKVLKREMNNIENTYKAFNSIATNKGSGTEGVGNKTIDGIKLEMIKKYHKEY
VNNQYNPQPVKKVLIPKGKNKTRPLGIPTIKDRIIQKAMEQLLTLYFENIFLEWSFGFRSKKSCHDAVKRVKQRFKGIDY
IIKIDIKGYFETINHDILNKMLNKYIRKAKTLKTINQWLKAGIMENGIKYESLSGTPQGGIISPLLSNIYLHYIDKKMEE
LIRNGKPIMKANPEYWKAYTKKQHHNLSIDSEINLNPNPRIEYIRYADDFIIGIKGEHHEAERIKTHVLKWLEQDLKLVV
NRDKSKIVKTTKGTRFLSYMVKVNPTNKTRKKKTTKNSLNGQVQIQIPKDTTRDYGKEYGWTKNGKVKHDETLASREALE
IIRTYKSITLGIIRYFCLGNNLAQLTHLYYLAEYSCLKTLVRKWKTSIAKVRRKLRNGKTWGIQYNLKNKTKVDSWDKLD
WEKIKRMRKTKGNPDVIINCYLYQGRTALVADSTVNTPQLT

Sequences:

>Translated_521_residues
MLTHNSNEFKLSRTLDMILNHSKNNYSLKKVLKREMNNIENTYKAFNSIATNKGSGTEGVGNKTIDGIKLEMIKKYHKEY
VNNQYNPQPVKKVLIPKGKNKTRPLGIPTIKDRIIQKAMEQLLTLYFENIFLEWSFGFRSKKSCHDAVKRVKQRFKGIDY
IIKIDIKGYFETINHDILNKMLNKYIRKAKTLKTINQWLKAGIMENGIKYESLSGTPQGGIISPLLSNIYLHYIDKKMEE
LIRNGKPIMKANPEYWKAYTKKQHHNLSIDSEINLNPNPRIEYIRYADDFIIGIKGEHHEAERIKTHVLKWLEQDLKLVV
NRDKSKIVKTTKGTRFLSYMVKVNPTNKTRKKKTTKNSLNGQVQIQIPKDTTRDYGKEYGWTKNGKVKHDETLASREALE
IIRTYKSITLGIIRYFCLGNNLAQLTHLYYLAEYSCLKTLVRKWKTSIAKVRRKLRNGKTWGIQYNLKNKTKVDSWDKLD
WEKIKRMRKTKGNPDVIINCYLYQGRTALVADSTVNTPQLT
>Mature_521_residues
MLTHNSNEFKLSRTLDMILNHSKNNYSLKKVLKREMNNIENTYKAFNSIATNKGSGTEGVGNKTIDGIKLEMIKKYHKEY
VNNQYNPQPVKKVLIPKGKNKTRPLGIPTIKDRIIQKAMEQLLTLYFENIFLEWSFGFRSKKSCHDAVKRVKQRFKGIDY
IIKIDIKGYFETINHDILNKMLNKYIRKAKTLKTINQWLKAGIMENGIKYESLSGTPQGGIISPLLSNIYLHYIDKKMEE
LIRNGKPIMKANPEYWKAYTKKQHHNLSIDSEINLNPNPRIEYIRYADDFIIGIKGEHHEAERIKTHVLKWLEQDLKLVV
NRDKSKIVKTTKGTRFLSYMVKVNPTNKTRKKKTTKNSLNGQVQIQIPKDTTRDYGKEYGWTKNGKVKHDETLASREALE
IIRTYKSITLGIIRYFCLGNNLAQLTHLYYLAEYSCLKTLVRKWKTSIAKVRRKLRNGKTWGIQYNLKNKTKVDSWDKLD
WEKIKRMRKTKGNPDVIINCYLYQGRTALVADSTVNTPQLT

Specific function: Multifunctional protein that promotes group II intron splicing and mobility by acting both on RNA and DNA. It has three activities:reverse transcriptase (RT) for intron duplication, maturase to promote splicing, and DNA endonuclease for site- specific cle

COG id: COG3344

COG function: function code L; Retron-type reverse transcriptase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 reverse transcriptase domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6226521, Length=522, Percent_Identity=29.1187739463602, Blast_Score=191, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6226520, Length=443, Percent_Identity=30.6997742663657, Blast_Score=189, Evalue=7e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003615
- InterPro:   IPR002866
- InterPro:   IPR000477 [H]

Pfam domain/function: PF01348 Intron_maturas2; PF00078 RVT_1 [H]

EC number: =2.7.7.49 [H]

Molecular weight: Translated: 60846; Mature: 60846

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: PS50878 RT_POL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTHNSNEFKLSRTLDMILNHSKNNYSLKKVLKREMNNIENTYKAFNSIATNKGSGTEGV
CCCCCCCCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GNKTIDGIKLEMIKKYHKEYVNNQYNPQPVKKVLIPKGKNKTRPLGIPTIKDRIIQKAME
CCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
QLLTLYFENIFLEWSFGFRSKKSCHDAVKRVKQRFKGIDYIIKIDIKGYFETINHDILNK
HHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHH
MLNKYIRKAKTLKTINQWLKAGIMENGIKYESLSGTPQGGIISPLLSNIYLHYIDKKMEE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LIRNGKPIMKANPEYWKAYTKKQHHNLSIDSEINLNPNPRIEYIRYADDFIIGIKGEHHE
HHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCEECCCCCCCEEEEEECCCEEEEECCCCCH
AERIKTHVLKWLEQDLKLVVNRDKSKIVKTTKGTRFLSYMVKVNPTNKTRKKKTTKNSLN
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHCCC
GQVQIQIPKDTTRDYGKEYGWTKNGKVKHDETLASREALEIIRTYKSITLGIIRYFCLGN
CEEEEEECCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NLAQLTHLYYLAEYSCLKTLVRKWKTSIAKVRRKLRNGKTWGIQYNLKNKTKVDSWDKLD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCC
WEKIKRMRKTKGNPDVIINCYLYQGRTALVADSTVNTPQLT
HHHHHHHHHCCCCCCEEEEEEEECCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MLTHNSNEFKLSRTLDMILNHSKNNYSLKKVLKREMNNIENTYKAFNSIATNKGSGTEGV
CCCCCCCCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GNKTIDGIKLEMIKKYHKEYVNNQYNPQPVKKVLIPKGKNKTRPLGIPTIKDRIIQKAME
CCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
QLLTLYFENIFLEWSFGFRSKKSCHDAVKRVKQRFKGIDYIIKIDIKGYFETINHDILNK
HHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHH
MLNKYIRKAKTLKTINQWLKAGIMENGIKYESLSGTPQGGIISPLLSNIYLHYIDKKMEE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LIRNGKPIMKANPEYWKAYTKKQHHNLSIDSEINLNPNPRIEYIRYADDFIIGIKGEHHE
HHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCEECCCCCCCEEEEEECCCEEEEECCCCCH
AERIKTHVLKWLEQDLKLVVNRDKSKIVKTTKGTRFLSYMVKVNPTNKTRKKKTTKNSLN
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHCCC
GQVQIQIPKDTTRDYGKEYGWTKNGKVKHDETLASREALEIIRTYKSITLGIIRYFCLGN
CEEEEEECCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NLAQLTHLYYLAEYSCLKTLVRKWKTSIAKVRRKLRNGKTWGIQYNLKNKTKVDSWDKLD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCC
WEKIKRMRKTKGNPDVIINCYLYQGRTALVADSTVNTPQLT
HHHHHHHHHCCCCCCEEEEEEEECCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8655550; 11157264; 10488339; 9353259; 10413481; 11731786 [H]