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The map label for this gene is engD [H]

Identifier: 197294429

GI number: 197294429

Start: 362033

End: 363124

Strand: Reverse

Name: engD [H]

Synonym: PAa_0347

Alternate gene names: 197294429

Gene position: 363124-362033 (Counterclockwise)

Preceding gene: 197294430

Following gene: 197294428

Centisome position: 41.27

GC content: 27.93

Gene sequence:

>1092_bases
ATGAAAATAGGTATTATTGGTTTACCGAATGTGGGTAAATCAACTTTATTTAATGCTTTAACGAAAATGCAAGTTTTAGA
AGCTAACTATCCTTTTGCAACAATTGAACCTAACGTAGGAACAGTTGAGGTCCCTGACCCACGTTTAAAAAAATTATCCC
AAATTTTTCAATCTCAAAAAATAATTTCTGCTTTAATAGAATTTAAAGATATTGCAGGATTAGTAGCAGGTGCTTCTAAA
GGAGAAGGATTAGGAAATCAATTTTTAAGTCATATTCGCAATGTAGATGCTATTTGTCATGTTGTTAAATGTTTTGAAGA
CCCTAATATTTTGCATGTTATGGACAAAAATGACCCTTTAGAAGAAATTAAAATTATCGAAACCGAACTTGCTTTAGCAG
ATTTAGAACAAATTGAAAAACGTTTACAAAAACTTCAAAAACAAAAAAAGAAAGGCGATAAAGATTTATTAAAAGAATAT
AACGTTTTAACGAAAATTCAACATCATTTAACAAACGAAGATTTAAAAAACCTTTCTTTAAACGAAGAAGAATTAAAAAT
AGTGAAAAACTTTAATTTATTATTTTTAAAACCTTCACTATATCTGGCTAATATCAAAGAAAATGATTTAGTAAACAACA
AAAACTTGCTTTTACAAAAAGTATTGGATTATGGAAAACAAAAAGGCAAAAAAGTGATTTCTTTATGTGTCCAATTAGAA
AAAGATTTATCTTTTTTGGAAGAAGAAGAAGAAGAAAAAGTTTTTTTAAAAGATTATGGGATGACTCAAACAGCTTTTGC
AAAATTAATTCAAGAAAGCTACAATCTTTTAGGTTTACAAACTTATTTTACTGCAGGTCCTAAAGAAGTGCACGCTTGGT
CTTTCCAAAAAGGATTAAAAGCTCCTGAATGTGCAAGAATTATTCACACCGATTTAGAAAAAGGGTTTATTAAAGCCGAA
GTACTTGCATATGAAGATTTAATCACTCATCAAACTTTTCAAAAAAGCAAAGAAAAAGGAAAATTAAGAATCGAAGGTAA
GGAATATATAGTAAAAGATGGCGATATTATTACTTTTAGGTTTAATGTATAA

Upstream 100 bases:

>100_bases
AGTTTTCAAATTTTGGTAGAAAAAAATAATCTGATAATTATTAAATTTTTAAAAGCAATAAATTACTATAAATAGATTTA
ATAGAAGGAATTTAAAAATT

Downstream 100 bases:

>100_bases
AAAATAAAATAATTCAAATTCCAAGAAAGAAATAAAATTATGCAAAATTATAAAAAGACTCTTTTAATGCCTCAAACTGA
TTTTCCGATGAAAGGAAATT

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MKIGIIGLPNVGKSTLFNALTKMQVLEANYPFATIEPNVGTVEVPDPRLKKLSQIFQSQKIISALIEFKDIAGLVAGASK
GEGLGNQFLSHIRNVDAICHVVKCFEDPNILHVMDKNDPLEEIKIIETELALADLEQIEKRLQKLQKQKKKGDKDLLKEY
NVLTKIQHHLTNEDLKNLSLNEEELKIVKNFNLLFLKPSLYLANIKENDLVNNKNLLLQKVLDYGKQKGKKVISLCVQLE
KDLSFLEEEEEEKVFLKDYGMTQTAFAKLIQESYNLLGLQTYFTAGPKEVHAWSFQKGLKAPECARIIHTDLEKGFIKAE
VLAYEDLITHQTFQKSKEKGKLRIEGKEYIVKDGDIITFRFNV

Sequences:

>Translated_363_residues
MKIGIIGLPNVGKSTLFNALTKMQVLEANYPFATIEPNVGTVEVPDPRLKKLSQIFQSQKIISALIEFKDIAGLVAGASK
GEGLGNQFLSHIRNVDAICHVVKCFEDPNILHVMDKNDPLEEIKIIETELALADLEQIEKRLQKLQKQKKKGDKDLLKEY
NVLTKIQHHLTNEDLKNLSLNEEELKIVKNFNLLFLKPSLYLANIKENDLVNNKNLLLQKVLDYGKQKGKKVISLCVQLE
KDLSFLEEEEEEKVFLKDYGMTQTAFAKLIQESYNLLGLQTYFTAGPKEVHAWSFQKGLKAPECARIIHTDLEKGFIKAE
VLAYEDLITHQTFQKSKEKGKLRIEGKEYIVKDGDIITFRFNV
>Mature_363_residues
MKIGIIGLPNVGKSTLFNALTKMQVLEANYPFATIEPNVGTVEVPDPRLKKLSQIFQSQKIISALIEFKDIAGLVAGASK
GEGLGNQFLSHIRNVDAICHVVKCFEDPNILHVMDKNDPLEEIKIIETELALADLEQIEKRLQKLQKQKKKGDKDLLKEY
NVLTKIQHHLTNEDLKNLSLNEEELKIVKNFNLLFLKPSLYLANIKENDLVNNKNLLLQKVLDYGKQKGKKVISLCVQLE
KDLSFLEEEEEEKVFLKDYGMTQTAFAKLIQESYNLLGLQTYFTAGPKEVHAWSFQKGLKAPECARIIHTDLEKGFIKAE
VLAYEDLITHQTFQKSKEKGKLRIEGKEYIVKDGDIITFRFNV

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=369, Percent_Identity=43.360433604336, Blast_Score=284, Evalue=1e-76,
Organism=Homo sapiens, GI58761502, Length=217, Percent_Identity=39.1705069124424, Blast_Score=147, Evalue=1e-35,
Organism=Escherichia coli, GI1787454, Length=364, Percent_Identity=49.7252747252747, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI1789574, Length=128, Percent_Identity=32.8125, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17509631, Length=367, Percent_Identity=40.3269754768392, Blast_Score=258, Evalue=4e-69,
Organism=Caenorhabditis elegans, GI17552324, Length=139, Percent_Identity=33.0935251798561, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6319499, Length=370, Percent_Identity=41.0810810810811, Blast_Score=261, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6321773, Length=379, Percent_Identity=35.0923482849604, Blast_Score=211, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6321649, Length=159, Percent_Identity=35.8490566037736, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24640873, Length=368, Percent_Identity=40.4891304347826, Blast_Score=273, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24640877, Length=368, Percent_Identity=40.4891304347826, Blast_Score=273, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24640875, Length=368, Percent_Identity=40.4891304347826, Blast_Score=273, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24640879, Length=327, Percent_Identity=38.8379204892966, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24585318, Length=196, Percent_Identity=31.1224489795918, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 41355; Mature: 41355

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGIIGLPNVGKSTLFNALTKMQVLEANYPFATIEPNVGTVEVPDPRLKKLSQIFQSQK
CEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCHHHHHHHHHHHHHH
IISALIEFKDIAGLVAGASKGEGLGNQFLSHIRNVDAICHVVKCFEDPNILHVMDKNDPL
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCH
EEIKIIETELALADLEQIEKRLQKLQKQKKKGDKDLLKEYNVLTKIQHHLTNEDLKNLSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHCCCC
NEEELKIVKNFNLLFLKPSLYLANIKENDLVNNKNLLLQKVLDYGKQKGKKVISLCVQLE
CHHHHHHHHCCCEEEEECCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KDLSFLEEEEEEKVFLKDYGMTQTAFAKLIQESYNLLGLQTYFTAGPKEVHAWSFQKGLK
HHHHHHHCHHHCEEEHHHCCCCHHHHHHHHHHHHHHEEEHHHHCCCCHHHHHHHHHHCCC
APECARIIHTDLEKGFIKAEVLAYEDLITHQTFQKSKEKGKLRIEGKEYIVKDGDIITFR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEECCCEEEEE
FNV
ECC
>Mature Secondary Structure
MKIGIIGLPNVGKSTLFNALTKMQVLEANYPFATIEPNVGTVEVPDPRLKKLSQIFQSQK
CEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCHHHHHHHHHHHHHH
IISALIEFKDIAGLVAGASKGEGLGNQFLSHIRNVDAICHVVKCFEDPNILHVMDKNDPL
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCH
EEIKIIETELALADLEQIEKRLQKLQKQKKKGDKDLLKEYNVLTKIQHHLTNEDLKNLSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHCCCC
NEEELKIVKNFNLLFLKPSLYLANIKENDLVNNKNLLLQKVLDYGKQKGKKVISLCVQLE
CHHHHHHHHCCCEEEEECCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KDLSFLEEEEEEKVFLKDYGMTQTAFAKLIQESYNLLGLQTYFTAGPKEVHAWSFQKGLK
HHHHHHHCHHHCEEEHHHCCCCHHHHHHHHHHHHHHEEEHHHHCCCCHHHHHHHHHHCCC
APECARIIHTDLEKGFIKAEVLAYEDLITHQTFQKSKEKGKLRIEGKEYIVKDGDIITFR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEECCCEEEEE
FNV
ECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]