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The map label for this gene is artI [H]

Identifier: 197294378

GI number: 197294378

Start: 296511

End: 297575

Strand: Reverse

Name: artI [H]

Synonym: PAa_0267

Alternate gene names: 197294378

Gene position: 297575-296511 (Counterclockwise)

Preceding gene: 197294379

Following gene: 197294377

Centisome position: 33.82

GC content: 25.26

Gene sequence:

>1065_bases
TTGCCTGCTGTATTAGATTTAATTAAAAAAGGCAACACTGATGCTTTTGTCAATGACACTCCTGTTATTCAAAATATTGT
CAATAATTTTCCTAATGATTTTGCAAGTGTTGAATTAAATAATAATGTCTTTGGTGATTTAAAACCTTTTGGTCTTTTTG
TTAATTCTTCCTACCCAAATAAAGCCAAAATTAAAGAAGCTTTATTAAAAGTTTTAGATTTAAATACAAAACCTTTAAAA
TCAGAAGATGAAATTGATGCCATATCAGATGCATATAAAGACATTTTTTTGAATAAAGCTATTAAAAATTATCAACAAAT
ACAACAAAAACTAAAAAATCAAAAACCCTTTCCAGGATTTATCAAGTTAACTAAAAAAATATGGTCCACTTTGCCAACTT
ACAAAAAAGGTTTTATTACTTCTTTCATCATTTCCATAGATAGCGTTATTTTAGGTTTTCTTTTAGCTTTGATATTTGTC
AAAACCAAGACATTAATTAAACACTATCAAAAAACAAACCAAAAAAATTTAATGCGTGTTTATCGAACTTTTTCTTTTTT
CATTAACAAAACAATCAGTTTTTTAAATGCTATTCCTTTAACAATTCAAGCATTATTGGTTTTTAATCTTTTCAATTTAT
TTCAAATATTAAGAAATAATACAATGAACTTTTATGCCGCTTTGTTTGTTCTTGTTTTAAATACAGCCGCCAATTTAGTT
AAAATTATGATGCATAATATTAAAATATTGTCTCAAGGACAAATTGAGGCAGGATACGCATTAGGAATGAGCCATAAACA
AGTTTTTCACTATATTATTTTCGAACAAGGGATTAAAAGATCTTATCCTTCTGTGGGACAACAATTTATCAATAATATCA
AAAATACATCTTTATTCCAAATTATTGGTTTATCAAGTCTTTTATTGCAAGCTCAAAGAAATACAACTCTAGATTTTGAT
TTATGGACTCCTTATATTATAGTTTGTGCCATTTATTTAATTATTGTTTTTATTACCAATTTTATTTTAGATAAAATCAA
AAAACAAAACAAAATTGAGTTTTAG

Upstream 100 bases:

>100_bases
AATAATTACTTTTCCAAGTTCTCTTTATGTTCAAAAATTAAAAGAATATTTCGAAAATAACCCAAAATTTCAAGTAGATG
AAAATAATTTTAGAGATAAT

Downstream 100 bases:

>100_bases
AAGTAATTAATTCAAAGCTTTTTTAACGCTTGCAGTTTAGTTTTTGAAAATTTTTTTCATTGTTGCTTTTTTGAGACAAC
TTTTTTATTTTTTCTTGCAA

Product: ABC-type arginine transport system,permease component

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MPAVLDLIKKGNTDAFVNDTPVIQNIVNNFPNDFASVELNNNVFGDLKPFGLFVNSSYPNKAKIKEALLKVLDLNTKPLK
SEDEIDAISDAYKDIFLNKAIKNYQQIQQKLKNQKPFPGFIKLTKKIWSTLPTYKKGFITSFIISIDSVILGFLLALIFV
KTKTLIKHYQKTNQKNLMRVYRTFSFFINKTISFLNAIPLTIQALLVFNLFNLFQILRNNTMNFYAALFVLVLNTAANLV
KIMMHNIKILSQGQIEAGYALGMSHKQVFHYIIFEQGIKRSYPSVGQQFINNIKNTSLFQIIGLSSLLLQAQRNTTLDFD
LWTPYIIVCAIYLIIVFITNFILDKIKKQNKIEF

Sequences:

>Translated_354_residues
MPAVLDLIKKGNTDAFVNDTPVIQNIVNNFPNDFASVELNNNVFGDLKPFGLFVNSSYPNKAKIKEALLKVLDLNTKPLK
SEDEIDAISDAYKDIFLNKAIKNYQQIQQKLKNQKPFPGFIKLTKKIWSTLPTYKKGFITSFIISIDSVILGFLLALIFV
KTKTLIKHYQKTNQKNLMRVYRTFSFFINKTISFLNAIPLTIQALLVFNLFNLFQILRNNTMNFYAALFVLVLNTAANLV
KIMMHNIKILSQGQIEAGYALGMSHKQVFHYIIFEQGIKRSYPSVGQQFINNIKNTSLFQIIGLSSLLLQAQRNTTLDFD
LWTPYIIVCAIYLIIVFITNFILDKIKKQNKIEF
>Mature_353_residues
PAVLDLIKKGNTDAFVNDTPVIQNIVNNFPNDFASVELNNNVFGDLKPFGLFVNSSYPNKAKIKEALLKVLDLNTKPLKS
EDEIDAISDAYKDIFLNKAIKNYQQIQQKLKNQKPFPGFIKLTKKIWSTLPTYKKGFITSFIISIDSVILGFLLALIFVK
TKTLIKHYQKTNQKNLMRVYRTFSFFINKTISFLNAIPLTIQALLVFNLFNLFQILRNNTMNFYAALFVLVLNTAANLVK
IMMHNIKILSQGQIEAGYALGMSHKQVFHYIIFEQGIKRSYPSVGQQFINNIKNTSLFQIIGLSSLLLQAQRNTTLDFDL
WTPYIIVCAIYLIIVFITNFILDKIKKQNKIEF

Specific function: Part of the binding-protein-dependent transport system for glutamine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787030, Length=180, Percent_Identity=30.5555555555556, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1787087, Length=123, Percent_Identity=30.8943089430894, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788226, Length=220, Percent_Identity=25.9090909090909, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 40605; Mature: 40474

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAVLDLIKKGNTDAFVNDTPVIQNIVNNFPNDFASVELNNNVFGDLKPFGLFVNSSYPN
CCHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCHHEEEEECCCCC
KAKIKEALLKVLDLNTKPLKSEDEIDAISDAYKDIFLNKAIKNYQQIQQKLKNQKPFPGF
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
IKLTKKIWSTLPTYKKGFITSFIISIDSVILGFLLALIFVKTKTLIKHYQKTNQKNLMRV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
YRTFSFFINKTISFLNAIPLTIQALLVFNLFNLFQILRNNTMNFYAALFVLVLNTAANLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
KIMMHNIKILSQGQIEAGYALGMSHKQVFHYIIFEQGIKRSYPSVGQQFINNIKNTSLFQ
HHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHH
IIGLSSLLLQAQRNTTLDFDLWTPYIIVCAIYLIIVFITNFILDKIKKQNKIEF
HHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PAVLDLIKKGNTDAFVNDTPVIQNIVNNFPNDFASVELNNNVFGDLKPFGLFVNSSYPN
CHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCHHEEEEECCCCC
KAKIKEALLKVLDLNTKPLKSEDEIDAISDAYKDIFLNKAIKNYQQIQQKLKNQKPFPGF
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
IKLTKKIWSTLPTYKKGFITSFIISIDSVILGFLLALIFVKTKTLIKHYQKTNQKNLMRV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
YRTFSFFINKTISFLNAIPLTIQALLVFNLFNLFQILRNNTMNFYAALFVLVLNTAANLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
KIMMHNIKILSQGQIEAGYALGMSHKQVFHYIIFEQGIKRSYPSVGQQFINNIKNTSLFQ
HHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHH
IIGLSSLLLQAQRNTTLDFDLWTPYIIVCAIYLIIVFITNFILDKIKKQNKIEF
HHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]