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The map label for this gene is dam [H]

Identifier: 197294370

GI number: 197294370

Start: 283999

End: 284811

Strand: Reverse

Name: dam [H]

Synonym: PAa_0250

Alternate gene names: 197294370

Gene position: 284811-283999 (Counterclockwise)

Preceding gene: 197294371

Following gene: 197294369

Centisome position: 32.37

GC content: 28.17

Gene sequence:

>813_bases
ATGAAAATAGCGTGGATAGGCAGTAAAAAGAAAATGTTACCCCAATTATTAAACTATATTCCAACAAATTATCATAATTA
TTATGAACCTTTTTTAGGAAGTGGGGCTTTATTCCAATCTCTTACTCCCTCAACTGTCACTTTGAACGATAATGATATCA
ATTTAATACAACTTTGGAAAGATATGTTAGCAAAACCAGAATTGTTTTGTGATTATGTGCGTAAATTTGAAGACAAAATA
TATGTAAATAAAGATCAAAAGACGCAAAAAGAATCATTTAAACATTTGGTAGAAAAATTTAATAAAAGTAATTTGGGTGC
TTATAAAAGTGCCCTTTTTTATGTTTTATTGAAATATGCTTTTAGGGGCATTTTCCGATATCGATCTGATGGAACTATTT
ATTTAGGATACAGTTTTTCATCTCGTTTCAAAAACCCTATAATTGTTTTATCAGAAATCCAAAGAATAAAAACCAATGTT
AAGAATATAAAATTGTTAAATGGCGATTTTGAAAGTGTCATTAACCAAACCCAAAGCAACGATTTTATATTTGTTGATCC
TCCGTATTGGCGTGAAGGGACTAAAGATAAAGACTTTTATCAAAAACCTTTCACATTTGAAGATCACAAAAGACTTTATA
AAAGTTTAGAGATGGCAAATTATCGCAAGGTAAAGTGGTTGTATACAAATTATAATTCCCCTAAAATAATTGAATTATTT
AAAGATTTTCATATCCAACCTCTCCAAACTACAACCAATTACGCTTTAACTTCAAAAGGAATAACACAAGAAGTTATTAT
TAAAAATTATTAA

Upstream 100 bases:

>100_bases
CTATTTTTTGATTTTAATTTAGGAATTAAGGCTAATACCGCTATTAGTCTTGTTTCTGATGTTGTCTTTTTAATTTATAT
TTGGGTAAAGGAGCTTAATA

Downstream 100 bases:

>100_bases
AAAGAACAAATAATGAAAATTTTAATCAACAAACAAATATTTTAATTAAAGGAGCATAAATAATATGAATTATCAAAAAA
CTTTTTATCGCAAAGGAATC

Product: Site-specific DNA methylase

Products: NA

Alternate protein names: M.LlaDCHI A; M.LlaDCHIA; Adenine-specific methyltransferase LlaDCHIA; M.LlaII A [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MKIAWIGSKKKMLPQLLNYIPTNYHNYYEPFLGSGALFQSLTPSTVTLNDNDINLIQLWKDMLAKPELFCDYVRKFEDKI
YVNKDQKTQKESFKHLVEKFNKSNLGAYKSALFYVLLKYAFRGIFRYRSDGTIYLGYSFSSRFKNPIIVLSEIQRIKTNV
KNIKLLNGDFESVINQTQSNDFIFVDPPYWREGTKDKDFYQKPFTFEDHKRLYKSLEMANYRKVKWLYTNYNSPKIIELF
KDFHIQPLQTTTNYALTSKGITQEVIIKNY

Sequences:

>Translated_270_residues
MKIAWIGSKKKMLPQLLNYIPTNYHNYYEPFLGSGALFQSLTPSTVTLNDNDINLIQLWKDMLAKPELFCDYVRKFEDKI
YVNKDQKTQKESFKHLVEKFNKSNLGAYKSALFYVLLKYAFRGIFRYRSDGTIYLGYSFSSRFKNPIIVLSEIQRIKTNV
KNIKLLNGDFESVINQTQSNDFIFVDPPYWREGTKDKDFYQKPFTFEDHKRLYKSLEMANYRKVKWLYTNYNSPKIIELF
KDFHIQPLQTTTNYALTSKGITQEVIIKNY
>Mature_270_residues
MKIAWIGSKKKMLPQLLNYIPTNYHNYYEPFLGSGALFQSLTPSTVTLNDNDINLIQLWKDMLAKPELFCDYVRKFEDKI
YVNKDQKTQKESFKHLVEKFNKSNLGAYKSALFYVLLKYAFRGIFRYRSDGTIYLGYSFSSRFKNPIIVLSEIQRIKTNV
KNIKLLNGDFESVINQTQSNDFIFVDPPYWREGTKDKDFYQKPFTFEDHKRLYKSLEMANYRKVKWLYTNYNSPKIIELF
KDFHIQPLQTTTNYALTSKGITQEVIIKNY

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the LlaDCHI endonuclease [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 32073; Mature: 32073

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAWIGSKKKMLPQLLNYIPTNYHNYYEPFLGSGALFQSLTPSTVTLNDNDINLIQLWK
CEEEEECCHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHCCCCCEEEECCCCCHHHHHHH
DMLAKPELFCDYVRKFEDKIYVNKDQKTQKESFKHLVEKFNKSNLGAYKSALFYVLLKYA
HHHCCHHHHHHHHHHHHHCEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
FRGIFRYRSDGTIYLGYSFSSRFKNPIIVLSEIQRIKTNVKNIKLLNGDFESVINQTQSN
HHHHHEECCCCEEEEEECHHHHCCCCEEEHHHHHHHHHCHHHEEEECCCHHHHHHHCCCC
DFIFVDPPYWREGTKDKDFYQKPFTFEDHKRLYKSLEMANYRKVKWLYTNYNSPKIIELF
CEEEECCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHH
KDFHIQPLQTTTNYALTSKGITQEVIIKNY
HHCCCCCCCCCCCEEEECCCCCHHHHCCCC
>Mature Secondary Structure
MKIAWIGSKKKMLPQLLNYIPTNYHNYYEPFLGSGALFQSLTPSTVTLNDNDINLIQLWK
CEEEEECCHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHCCCCCEEEECCCCCHHHHHHH
DMLAKPELFCDYVRKFEDKIYVNKDQKTQKESFKHLVEKFNKSNLGAYKSALFYVLLKYA
HHHCCHHHHHHHHHHHHHCEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
FRGIFRYRSDGTIYLGYSFSSRFKNPIIVLSEIQRIKTNVKNIKLLNGDFESVINQTQSN
HHHHHEECCCCEEEEEECHHHHCCCCEEEHHHHHHHHHCHHHEEEECCCHHHHHHHCCCC
DFIFVDPPYWREGTKDKDFYQKPFTFEDHKRLYKSLEMANYRKVKWLYTNYNSPKIIELF
CEEEECCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHH
KDFHIQPLQTTTNYALTSKGITQEVIIKNY
HHCCCCCCCCCCCEEEECCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7793939; 11467810 [H]