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The map label for this gene is gtfA [C]

Identifier: 197294346

GI number: 197294346

Start: 260515

End: 261975

Strand: Direct

Name: gtfA [C]

Synonym: PAa_0221

Alternate gene names: 197294346

Gene position: 260515-261975 (Clockwise)

Preceding gene: 197294343

Following gene: 197294350

Centisome position: 29.61

GC content: 30.6

Gene sequence:

>1461_bases
ATGAAAATTAAAAATAAAGTAATGTTGATCACTTATCCTGATTCATTGGGAAAAAACATTTCAGATTTAATTAAAGTTTT
AAAAGAAGATATTGGCAACGATGTTATTGGAGGAGTTCATCTTTTGCCTTTTTTCCCCTCCACAAGTGACCGCGGCTTTG
CACCTTCTGATTATAATTTAGTTGATCCTGCTTTTGGAGATTGGAAAGATATAGAAGAATTAGGTAAACAATATTATTTA
ATGTTTGATTTTATGATTAATCATATTTCACGTGCATCTGTCATGTATCAAGATTTCAAAAAGAATCATGAATTATCCAA
ATATAGTAATTTTTTTATTCGTTGGGAAAATTTTTGGTTAAAAGCAGGTCAAAACAGACCTACACAAGAAGATATTGATT
TGATTTATAAACGAAAGGACAAAGAACCCAACCAACTTATTAAGTTTGATAACGGCCAAAAAGAACACCTTTGGAACACT
TTTGGCGAAGATCAAATTGATATCAACATCAATGATGATATTGCCAAAGAATTTATTAAAAAAACTTTAGAATTTATGGT
GAAAAAAGGGGCTAGTTTAATTCGTTTAGATGCATTTGCTTATGTTTGTAAAAAAGCTGGTACAAATTGTTTTTTCTTAG
AACCTGAAATATGGGATTTGTTACATGGAGTAAGCGAGATTTTAAAACCTTTAGGAGCTTGTATATTGCCAGAAATTCAT
GAACAATATTCTATTTCTCATAAAATAAGCGAACAAGGATATTTTATTTATGATTTCGCGCTTCCTTTGGTAGTCCTTTT
TACGATGTATTCAGGAAAAACACATCGCTTGAAAGAATGGCTTGCAAAATCTCCCATGAAACAATTCACTACCCTAGATA
CTCACGACGGCATTGGGGTTGTTGATGCACTTGATATTCTTACAGAAGATGAAATTAATTATACCTCTAAAAGAATATAT
AAAATTGGTGTTAATGCTAAAAAAATTTATTCCACTAAAGCATACAAAAACCTAGATATTTATCAAATCAATTCAACTTA
TTATTCAGCCTTAGGCAATGAAGATGACACTTATTTATTAAGTCGTGCTTTTCAAATTTTTGCTCCTGGCATTCCTCAAA
TTTATTATGTTGGTCTTTTGGCAGGAGAAAACGATATTAAATTATTAGAGGCGACTAAAGAAGGCAGAAACATCAACCGT
CATTATTATTCTCGCGAAGAAATCAAAGCAGAAGTCAAAAGGCAAGTTGTAGTTGATTTATTTAAATTATTAAAGTGGCG
CAATGAATGTCAAGCCTTTGATTTAGATGGAAGTATTTTTGTTGATCCTACTTTAGAAAATCATAAGATCAAAATCTTAC
GTCAAGATAAAAGCGGTCAAAATAAAGCTGTTTTAGAAGCGGATGTTGTTAGCAAAAAATTTATTATTACTCACAACGGC
AAAGAAATATTTAAAAATTAA

Upstream 100 bases:

>100_bases
TCCTTTGTTTTTTTGTATTTATAATATTTTTTTTAACTATAGTACCAAATATCAAAATAAATTATATAATAAAAATATTT
TAAAAAAAGGGCATAAATAA

Downstream 100 bases:

>100_bases
GTAAATTTGTTAAAAAAATAAACTCTCACAGTTAAAAGCTGCAAGAGTTTATTTCTTTTTTTATTTGGCTGAAAGAATTG
CTAAAAAAGCTTTTTGAGGT

Product: Sucrose phosphorylase (Glucosyltransferase-A)

Products: NA

Alternate protein names: Sucrose glucosyltransferase [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MKIKNKVMLITYPDSLGKNISDLIKVLKEDIGNDVIGGVHLLPFFPSTSDRGFAPSDYNLVDPAFGDWKDIEELGKQYYL
MFDFMINHISRASVMYQDFKKNHELSKYSNFFIRWENFWLKAGQNRPTQEDIDLIYKRKDKEPNQLIKFDNGQKEHLWNT
FGEDQIDININDDIAKEFIKKTLEFMVKKGASLIRLDAFAYVCKKAGTNCFFLEPEIWDLLHGVSEILKPLGACILPEIH
EQYSISHKISEQGYFIYDFALPLVVLFTMYSGKTHRLKEWLAKSPMKQFTTLDTHDGIGVVDALDILTEDEINYTSKRIY
KIGVNAKKIYSTKAYKNLDIYQINSTYYSALGNEDDTYLLSRAFQIFAPGIPQIYYVGLLAGENDIKLLEATKEGRNINR
HYYSREEIKAEVKRQVVVDLFKLLKWRNECQAFDLDGSIFVDPTLENHKIKILRQDKSGQNKAVLEADVVSKKFIITHNG
KEIFKN

Sequences:

>Translated_486_residues
MKIKNKVMLITYPDSLGKNISDLIKVLKEDIGNDVIGGVHLLPFFPSTSDRGFAPSDYNLVDPAFGDWKDIEELGKQYYL
MFDFMINHISRASVMYQDFKKNHELSKYSNFFIRWENFWLKAGQNRPTQEDIDLIYKRKDKEPNQLIKFDNGQKEHLWNT
FGEDQIDININDDIAKEFIKKTLEFMVKKGASLIRLDAFAYVCKKAGTNCFFLEPEIWDLLHGVSEILKPLGACILPEIH
EQYSISHKISEQGYFIYDFALPLVVLFTMYSGKTHRLKEWLAKSPMKQFTTLDTHDGIGVVDALDILTEDEINYTSKRIY
KIGVNAKKIYSTKAYKNLDIYQINSTYYSALGNEDDTYLLSRAFQIFAPGIPQIYYVGLLAGENDIKLLEATKEGRNINR
HYYSREEIKAEVKRQVVVDLFKLLKWRNECQAFDLDGSIFVDPTLENHKIKILRQDKSGQNKAVLEADVVSKKFIITHNG
KEIFKN
>Mature_486_residues
MKIKNKVMLITYPDSLGKNISDLIKVLKEDIGNDVIGGVHLLPFFPSTSDRGFAPSDYNLVDPAFGDWKDIEELGKQYYL
MFDFMINHISRASVMYQDFKKNHELSKYSNFFIRWENFWLKAGQNRPTQEDIDLIYKRKDKEPNQLIKFDNGQKEHLWNT
FGEDQIDININDDIAKEFIKKTLEFMVKKGASLIRLDAFAYVCKKAGTNCFFLEPEIWDLLHGVSEILKPLGACILPEIH
EQYSISHKISEQGYFIYDFALPLVVLFTMYSGKTHRLKEWLAKSPMKQFTTLDTHDGIGVVDALDILTEDEINYTSKRIY
KIGVNAKKIYSTKAYKNLDIYQINSTYYSALGNEDDTYLLSRAFQIFAPGIPQIYYVGLLAGENDIKLLEATKEGRNINR
HYYSREEIKAEVKRQVVVDLFKLLKWRNECQAFDLDGSIFVDPTLENHKIKILRQDKSGQNKAVLEADVVSKKFIITHNG
KEIFKN

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sucrose phosphorylase family [H]

Homologues:

Organism=Escherichia coli, GI87081873, Length=487, Percent_Identity=27.7207392197125, Blast_Score=174, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR016377
- InterPro:   IPR022527 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =2.4.1.7 [H]

Molecular weight: Translated: 56344; Mature: 56344

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKNKVMLITYPDSLGKNISDLIKVLKEDIGNDVIGGVHLLPFFPSTSDRGFAPSDYNL
CCCCCCEEEEECCHHHCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCC
VDPAFGDWKDIEELGKQYYLMFDFMINHISRASVMYQDFKKNHELSKYSNFFIRWENFWL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCEEEEEEEEEE
KAGQNRPTQEDIDLIYKRKDKEPNQLIKFDNGQKEHLWNTFGEDQIDININDDIAKEFIK
ECCCCCCCHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHCCCCCEEEEECCHHHHHHHHH
KTLEFMVKKGASLIRLDAFAYVCKKAGTNCFFLEPEIWDLLHGVSEILKPLGACILPEIH
HHHHHHHHCCCCEEEHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCHHHH
EQYSISHKISEQGYFIYDFALPLVVLFTMYSGKTHRLKEWLAKSPMKQFTTLDTHDGIGV
HHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCH
VDALDILTEDEINYTSKRIYKIGVNAKKIYSTKAYKNLDIYQINSTYYSALGNEDDTYLL
HHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCEEEEECCHHHHHCCCCCCHHHH
SRAFQIFAPGIPQIYYVGLLAGENDIKLLEATKEGRNINRHYYSREEIKAEVKRQVVVDL
HHHHHHHCCCCCCEEEEEEEECCCCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHHHHHH
FKLLKWRNECQAFDLDGSIFVDPTLENHKIKILRQDKSGQNKAVLEADVVSKKFIITHNG
HHHHHHHHHCCEEECCCCEEECCCCCCCEEEEEECCCCCCCCEEEEHHHCCCEEEEEECC
KEIFKN
HHHHCC
>Mature Secondary Structure
MKIKNKVMLITYPDSLGKNISDLIKVLKEDIGNDVIGGVHLLPFFPSTSDRGFAPSDYNL
CCCCCCEEEEECCHHHCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCC
VDPAFGDWKDIEELGKQYYLMFDFMINHISRASVMYQDFKKNHELSKYSNFFIRWENFWL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCEEEEEEEEEE
KAGQNRPTQEDIDLIYKRKDKEPNQLIKFDNGQKEHLWNTFGEDQIDININDDIAKEFIK
ECCCCCCCHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHCCCCCEEEEECCHHHHHHHHH
KTLEFMVKKGASLIRLDAFAYVCKKAGTNCFFLEPEIWDLLHGVSEILKPLGACILPEIH
HHHHHHHHCCCCEEEHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCHHHH
EQYSISHKISEQGYFIYDFALPLVVLFTMYSGKTHRLKEWLAKSPMKQFTTLDTHDGIGV
HHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCH
VDALDILTEDEINYTSKRIYKIGVNAKKIYSTKAYKNLDIYQINSTYYSALGNEDDTYLL
HHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCEEEEECCHHHHHCCCCCCHHHH
SRAFQIFAPGIPQIYYVGLLAGENDIKLLEATKEGRNINRHYYSREEIKAEVKRQVVVDL
HHHHHHHCCCCCCEEEEEEEECCCCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHHHHHH
FKLLKWRNECQAFDLDGSIFVDPTLENHKIKILRQDKSGQNKAVLEADVVSKKFIITHNG
HHHHHHHHHCCEEECCCCEEECCCCCCCEEEEEECCCCCCCCEEEEHHHCCCEEEEEECC
KEIFKN
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1368718 [H]