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The map label for this gene is dnaB [H]

Identifier: 197294220

GI number: 197294220

Start: 80418

End: 81701

Strand: Reverse

Name: dnaB [H]

Synonym: PAa_0073

Alternate gene names: 197294220

Gene position: 81701-80418 (Counterclockwise)

Preceding gene: 197294222

Following gene: 197294219

Centisome position: 9.28

GC content: 30.45

Gene sequence:

>1284_bases
ATGATTGAAGCCGAACGTGCATTATTGGGAACACTATTATTAAAACCAGAAAAAATGGATGTTGTTATAAATTTAGTCAA
CACAAACAATTTTTCCAACCCCAAGCATCGTTGTATCTTTGAGACAATGAAGCAATTAAAACTGCAAAATCGCGAAATTG
ATTATGTTACGGTGGGTTCCGTTTTAGATAACAATTTATACAAAATAGGAGGAATTGATTATTTAATTGAATTAGTGGAG
ACGTCCCCTGCAAGTGAATATTTAGAAACTTACATTGATTTAATTAAAGAAAATGCCCTTAAAAGAGATTTGTTGGGATT
AATCAAGCAATTACCTACTGAATTATCAAAAAGTAAAAATATCCATAATTATTTGCAAGCAGTCAAAACCCAAGTAGAAA
GTTTTATGCAAAAAACTAAATCTCCTTTCATTTCTACAAAAAAATTAATTCCTTATATTCGCCAAAGTATAGTTAATGAT
GACAAAGACAACCAATTTGTAGGAATAAAAACAGGTTTCAAAAATCTAGATGAATTAATTTCTGGTTTCAAACCAAAGGA
ATTAATTATCTTAGGAGCAAGAACTGGGATGGGGAAAACTGCTTTTATGTTAAATTTGGCTTGTAATATTGCAAAAAAAT
TTACTACCAACCCCGAAAACCAAAAAGCAGTTATTTTTAGTTTAGAAATGGCAGCAGAAGAATTAGGAATTAGGCTACTT
TCTTCTGCTTCTCAAATCCCTTTAAAACAACTCCAACACAAAAACTTAACTAAAAATGACAAATTACAGTTACTTATCGC
TGATGACAAAATTACGCAATTAAACATTTTAATTGATGATGATAGAAATAACAAAATTGAAGATATTAAAACCAAATGCC
GTCAAATGAAATATACTAAGGGGCTTGATATTGTCTTCATTGATTACCTCCATTTATTAAAAGACGACCAAAGTTTTAAT
TCATACCAAGCAATATCTGTGATTTCACGCAAATTAAAAAAACTAGCAAGTGAACTTAATATTCCAATTGTAGCTTTGAG
TCAAATGAACCGTGCAACCTATGTAAGAGAAGTTAAAAGCCCACAATTAACAGATTTAAGAGATTCGGGAACAATCGAAC
AAGACGCTGATGTTGTGATGTTATTACACCGCGAAAGTTATTATCAAAAACCTGATATTAACCCACATACGAATTTAATT
ATTGCCAAAAACCGTAGCGGTCAATTAGGTGAATGTAGTTTTAATTTTTATAAACAAATCCAAAGATTTGAAGCAAAAGA
ATAA

Upstream 100 bases:

>100_bases
GTACTGGTTGAGAACAACAAATTTATCCAAATTGTTTATTAATTAAAAATAAACAGTTTGGTTTTTTTTGTATCCAAACT
GAGAAAAAGGAGTTTGAAAG

Downstream 100 bases:

>100_bases
GAATAAAAAAGGAAATAAAAATATGAAACAAATATCTAATTTAGATTTTAATAATATTAACGTTTTTAATCATAAATCTT
TGTGCGTTAACATAACCAAA

Product: Replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MIEAERALLGTLLLKPEKMDVVINLVNTNNFSNPKHRCIFETMKQLKLQNREIDYVTVGSVLDNNLYKIGGIDYLIELVE
TSPASEYLETYIDLIKENALKRDLLGLIKQLPTELSKSKNIHNYLQAVKTQVESFMQKTKSPFISTKKLIPYIRQSIVND
DKDNQFVGIKTGFKNLDELISGFKPKELIILGARTGMGKTAFMLNLACNIAKKFTTNPENQKAVIFSLEMAAEELGIRLL
SSASQIPLKQLQHKNLTKNDKLQLLIADDKITQLNILIDDDRNNKIEDIKTKCRQMKYTKGLDIVFIDYLHLLKDDQSFN
SYQAISVISRKLKKLASELNIPIVALSQMNRATYVREVKSPQLTDLRDSGTIEQDADVVMLLHRESYYQKPDINPHTNLI
IAKNRSGQLGECSFNFYKQIQRFEAKE

Sequences:

>Translated_427_residues
MIEAERALLGTLLLKPEKMDVVINLVNTNNFSNPKHRCIFETMKQLKLQNREIDYVTVGSVLDNNLYKIGGIDYLIELVE
TSPASEYLETYIDLIKENALKRDLLGLIKQLPTELSKSKNIHNYLQAVKTQVESFMQKTKSPFISTKKLIPYIRQSIVND
DKDNQFVGIKTGFKNLDELISGFKPKELIILGARTGMGKTAFMLNLACNIAKKFTTNPENQKAVIFSLEMAAEELGIRLL
SSASQIPLKQLQHKNLTKNDKLQLLIADDKITQLNILIDDDRNNKIEDIKTKCRQMKYTKGLDIVFIDYLHLLKDDQSFN
SYQAISVISRKLKKLASELNIPIVALSQMNRATYVREVKSPQLTDLRDSGTIEQDADVVMLLHRESYYQKPDINPHTNLI
IAKNRSGQLGECSFNFYKQIQRFEAKE
>Mature_427_residues
MIEAERALLGTLLLKPEKMDVVINLVNTNNFSNPKHRCIFETMKQLKLQNREIDYVTVGSVLDNNLYKIGGIDYLIELVE
TSPASEYLETYIDLIKENALKRDLLGLIKQLPTELSKSKNIHNYLQAVKTQVESFMQKTKSPFISTKKLIPYIRQSIVND
DKDNQFVGIKTGFKNLDELISGFKPKELIILGARTGMGKTAFMLNLACNIAKKFTTNPENQKAVIFSLEMAAEELGIRLL
SSASQIPLKQLQHKNLTKNDKLQLLIADDKITQLNILIDDDRNNKIEDIKTKCRQMKYTKGLDIVFIDYLHLLKDDQSFN
SYQAISVISRKLKKLASELNIPIVALSQMNRATYVREVKSPQLTDLRDSGTIEQDADVVMLLHRESYYQKPDINPHTNLI
IAKNRSGQLGECSFNFYKQIQRFEAKE

Specific function: Participates In Initiation And Elongation During Chromosome Replication; It Exhibits DNA-Dependent ATPase Activity And Contains Distinct Active Sites For ATP Binding, DNA Binding, And Interaction With DNAc Protein, Primase, And Other Prepriming Proteins.

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=440, Percent_Identity=31.3636363636364, Blast_Score=215, Evalue=6e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 48822; Mature: 48822

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEAERALLGTLLLKPEKMDVVINLVNTNNFSNPKHRCIFETMKQLKLQNREIDYVTVGS
CCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECH
VLDNNLYKIGGIDYLIELVETSPASEYLETYIDLIKENALKRDLLGLIKQLPTELSKSKN
HHCCCCEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IHNYLQAVKTQVESFMQKTKSPFISTKKLIPYIRQSIVNDDKDNQFVGIKTGFKNLDELI
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHH
SGFKPKELIILGARTGMGKTAFMLNLACNIAKKFTTNPENQKAVIFSLEMAAEELGIRLL
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHH
SSASQIPLKQLQHKNLTKNDKLQLLIADDKITQLNILIDDDRNNKIEDIKTKCRQMKYTK
HCCCCCCHHHHHHCCCCCCCCEEEEEECCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCC
GLDIVFIDYLHLLKDDQSFNSYQAISVISRKLKKLASELNIPIVALSQMNRATYVREVKS
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHCHHHHHHHHHCC
PQLTDLRDSGTIEQDADVVMLLHRESYYQKPDINPHTNLIIAKNRSGQLGECSFNFYKQI
CCCCCCCCCCCCCCCCCEEEEEECHHCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHH
QRFEAKE
HHHHCCC
>Mature Secondary Structure
MIEAERALLGTLLLKPEKMDVVINLVNTNNFSNPKHRCIFETMKQLKLQNREIDYVTVGS
CCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECH
VLDNNLYKIGGIDYLIELVETSPASEYLETYIDLIKENALKRDLLGLIKQLPTELSKSKN
HHCCCCEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IHNYLQAVKTQVESFMQKTKSPFISTKKLIPYIRQSIVNDDKDNQFVGIKTGFKNLDELI
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHH
SGFKPKELIILGARTGMGKTAFMLNLACNIAKKFTTNPENQKAVIFSLEMAAEELGIRLL
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHH
SSASQIPLKQLQHKNLTKNDKLQLLIADDKITQLNILIDDDRNNKIEDIKTKCRQMKYTK
HCCCCCCHHHHHHCCCCCCCCEEEEEECCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCC
GLDIVFIDYLHLLKDDQSFNSYQAISVISRKLKKLASELNIPIVALSQMNRATYVREVKS
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHCHHHHHHHHHCC
PQLTDLRDSGTIEQDADVVMLLHRESYYQKPDINPHTNLIIAKNRSGQLGECSFNFYKQI
CCCCCCCCCCCCCCCCCEEEEEECHHCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHH
QRFEAKE
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 1312531 [H]