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The map label for this gene is lpdA-2 [H]

Identifier: 197120147

GI number: 197120147

Start: 4322286

End: 4323635

Strand: Direct

Name: lpdA-2 [H]

Synonym: Gbem_3786

Alternate gene names: 197120147

Gene position: 4322286-4323635 (Clockwise)

Preceding gene: 197120146

Following gene: 197120151

Centisome position: 93.65

GC content: 65.41

Gene sequence:

>1350_bases
ATGCAGGAAACGGCGGATGTGCTGGTGATCGGGACGGGGACGGCGGGTTTCACCCTCGCGCTTGCCTGCCGCAAGGGAGG
GCGGCAGGTCGCGGTGGTGGACGACAAGCCCTACGGCGGCACCTGCGGCCGCAACGGCTGCGAACCTGAGAAGTACCTGA
TGCAGGCGGCGCAGGTGGTGCATCTGACCCGGCAGATGTCGGGGCAGGGGATCACCGTTCCCGCTGCCATGGACTGGCCC
GCGCTGATCCGCTCCAAATCCGCTTTCAGCAACGGCGTGCCGGAGCGGACCGAGCGGGCTTTCCAGCAGGCGGGAATCAA
GATGTACTTCGGCACCGCCCACTTCCTCTCCCCCGAAACGGTCGCCATCGGCAGCGAGACCACCGTCCGCGCCGAAACCA
TCGTCATCGCCACCGGCGCCCGCCCCGCCCCCCTCGACTTCCCCGGTGCCGGCCTGGTGGTGGAAACCAGCGATTTCATG
GAGATGAAGAACCTGCCGCGCCGCGTCCTGTTCATCGGCGGCGGCTGCCTCGCGCTCAGCTTCGGGCACGTGGCGCGCGC
AGCTGGGGCCGACGTTACCATCCTGCAGCGCGGCGAGCGGGTATTGAAGAATTTCGACCTGGAAATGGCGCAACTGGCGG
CGAAGGCGGCCCGGGCCAGAGGCATCAACATAGTGACTGGAATAACCGCAGCCATGGCCGAGAAGGTCCAGGGCGCTTTC
ATGACCTACGGCAAGGGGGGATGCACCGAGGCCTTTCCCAGCGACCTCATCGTCAACACGTCGGGACGCATACCCGATCT
CGACCCGGTCGACCCGGAGGCGGGCGCTGTGGCGAGGAGCGCACGCGGGGTGACGGTGAACGAGTTCCTGCAGAGCGTCA
GCAACCCGCGCGTCTGGGCCATAGGCGATGCCTGCGACTCGCCATATCTGCTGTCAACCGTCGCGGACATGGAGGCGGAA
GTCGCCGCCGACAACATCCTGACCGGCAACCGGCGGCGCCCCGACTACCAGGGAGTCCCCAGCATGGCGCAGGCGCAGCC
TCCCCTATCCTTCGTAGGCCTCACCGAGGCACAGGCGAGGCAGTCGGGAAAGAAGTTCCGGATCAACCGGGGCTCCACCG
ATTCCTGGCCCTCGTCGCGCCGCATCGGTCAGCAGGGAGGCTTTTACAAGGTGCTCATCGAGGAAGAGACGGGAAAGATC
CTGGGGGCGCATCTTTTGGGGCAAAACGCCGGCGAGACGATCAACATTTTCGCCCTGGCTCTCAAGTTCGGGATCAGCAA
CAGCGAATTGCGCCAGATCCTCTGGACCTACCCCACCTTCATCTCAGACGTGAAAGACATGATCGAGTGA

Upstream 100 bases:

>100_bases
GTTGGAGATCATGCCGGACATCTCGGGATCGGCGGCCCGCGCCTCCTTGGGGCGGCTTCTGCTGGCCTGAAAAGTCCCGG
ATCCGACAAAGGAGGCGCTC

Downstream 100 bases:

>100_bases
ATACCCTGGCGGGAAAAAATGCGGACAAAAAAAAGCGCCGAGACGAAAGGAGGGCTCCATCATCGGCGCAAAGTAGGTTT
TGTTTTGTGCTGCTGTCTTT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MQETADVLVIGTGTAGFTLALACRKGGRQVAVVDDKPYGGTCGRNGCEPEKYLMQAAQVVHLTRQMSGQGITVPAAMDWP
ALIRSKSAFSNGVPERTERAFQQAGIKMYFGTAHFLSPETVAIGSETTVRAETIVIATGARPAPLDFPGAGLVVETSDFM
EMKNLPRRVLFIGGGCLALSFGHVARAAGADVTILQRGERVLKNFDLEMAQLAAKAARARGINIVTGITAAMAEKVQGAF
MTYGKGGCTEAFPSDLIVNTSGRIPDLDPVDPEAGAVARSARGVTVNEFLQSVSNPRVWAIGDACDSPYLLSTVADMEAE
VAADNILTGNRRRPDYQGVPSMAQAQPPLSFVGLTEAQARQSGKKFRINRGSTDSWPSSRRIGQQGGFYKVLIEEETGKI
LGAHLLGQNAGETINIFALALKFGISNSELRQILWTYPTFISDVKDMIE

Sequences:

>Translated_449_residues
MQETADVLVIGTGTAGFTLALACRKGGRQVAVVDDKPYGGTCGRNGCEPEKYLMQAAQVVHLTRQMSGQGITVPAAMDWP
ALIRSKSAFSNGVPERTERAFQQAGIKMYFGTAHFLSPETVAIGSETTVRAETIVIATGARPAPLDFPGAGLVVETSDFM
EMKNLPRRVLFIGGGCLALSFGHVARAAGADVTILQRGERVLKNFDLEMAQLAAKAARARGINIVTGITAAMAEKVQGAF
MTYGKGGCTEAFPSDLIVNTSGRIPDLDPVDPEAGAVARSARGVTVNEFLQSVSNPRVWAIGDACDSPYLLSTVADMEAE
VAADNILTGNRRRPDYQGVPSMAQAQPPLSFVGLTEAQARQSGKKFRINRGSTDSWPSSRRIGQQGGFYKVLIEEETGKI
LGAHLLGQNAGETINIFALALKFGISNSELRQILWTYPTFISDVKDMIE
>Mature_449_residues
MQETADVLVIGTGTAGFTLALACRKGGRQVAVVDDKPYGGTCGRNGCEPEKYLMQAAQVVHLTRQMSGQGITVPAAMDWP
ALIRSKSAFSNGVPERTERAFQQAGIKMYFGTAHFLSPETVAIGSETTVRAETIVIATGARPAPLDFPGAGLVVETSDFM
EMKNLPRRVLFIGGGCLALSFGHVARAAGADVTILQRGERVLKNFDLEMAQLAAKAARARGINIVTGITAAMAEKVQGAF
MTYGKGGCTEAFPSDLIVNTSGRIPDLDPVDPEAGAVARSARGVTVNEFLQSVSNPRVWAIGDACDSPYLLSTVADMEAE
VAADNILTGNRRRPDYQGVPSMAQAQPPLSFVGLTEAQARQSGKKFRINRGSTDSWPSSRRIGQQGGFYKVLIEEETGKI
LGAHLLGQNAGETINIFALALKFGISNSELRQILWTYPTFISDVKDMIE

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=455, Percent_Identity=26.1538461538462, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI50301238, Length=455, Percent_Identity=23.7362637362637, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI22035672, Length=445, Percent_Identity=26.0674157303371, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI33519430, Length=427, Percent_Identity=23.4192037470726, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI33519428, Length=427, Percent_Identity=23.4192037470726, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI33519426, Length=427, Percent_Identity=23.4192037470726, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI148277065, Length=427, Percent_Identity=23.4192037470726, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI148277071, Length=427, Percent_Identity=23.4192037470726, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI291045266, Length=442, Percent_Identity=23.3031674208145, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1789915, Length=420, Percent_Identity=26.1904761904762, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1786307, Length=451, Percent_Identity=26.6075388026608, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI87081717, Length=451, Percent_Identity=26.6075388026608, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI87082354, Length=428, Percent_Identity=26.1682242990654, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32565766, Length=454, Percent_Identity=27.5330396475771, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17557007, Length=464, Percent_Identity=26.0775862068966, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71983429, Length=438, Percent_Identity=22.1461187214612, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71983419, Length=438, Percent_Identity=22.1461187214612, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71982272, Length=472, Percent_Identity=23.728813559322, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321091, Length=464, Percent_Identity=25.6465517241379, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6325240, Length=474, Percent_Identity=25.1054852320675, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6325166, Length=453, Percent_Identity=22.2958057395143, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21358499, Length=453, Percent_Identity=27.5938189845475, Blast_Score=165, Evalue=6e-41,
Organism=Drosophila melanogaster, GI24640549, Length=461, Percent_Identity=26.8980477223427, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24640553, Length=461, Percent_Identity=26.8980477223427, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24640551, Length=461, Percent_Identity=26.8980477223427, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI17737741, Length=460, Percent_Identity=26.9565217391304, Blast_Score=116, Evalue=4e-26,

Paralogues:

None

Copy number: 650 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000815
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00403 HMA; PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 47950; Mature: 47950

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQETADVLVIGTGTAGFTLALACRKGGRQVAVVDDKPYGGTCGRNGCEPEKYLMQAAQVV
CCCCCCEEEEECCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
HLTRQMSGQGITVPAAMDWPALIRSKSAFSNGVPERTERAFQQAGIKMYFGTAHFLSPET
HHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEEEECCCCE
VAIGSETTVRAETIVIATGARPAPLDFPGAGLVVETSDFMEMKNLPRRVLFIGGGCLALS
EEECCCCEEEEEEEEEECCCCCCCCCCCCCCEEEECHHHHHHHCCCCEEEEECCCHHHHH
FGHVARAAGADVTILQRGERVLKNFDLEMAQLAAKAARARGINIVTGITAAMAEKVQGAF
HHHHHHHCCCCEEEHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCHH
MTYGKGGCTEAFPSDLIVNTSGRIPDLDPVDPEAGAVARSARGVTVNEFLQSVSNPRVWA
EECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHCCCCEEE
IGDACDSPYLLSTVADMEAEVAADNILTGNRRRPDYQGVPSMAQAQPPLSFVGLTEAQAR
ECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCEEEECCHHHHH
QSGKKFRINRGSTDSWPSSRRIGQQGGFYKVLIEEETGKILGAHLLGQNAGETINIFALA
HCCCEEEEECCCCCCCCCHHHHCCCCCEEEEEEECCCCCEEEHHHHCCCCCCEEEEEEEE
LKFGISNSELRQILWTYPTFISDVKDMIE
EHHCCCHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MQETADVLVIGTGTAGFTLALACRKGGRQVAVVDDKPYGGTCGRNGCEPEKYLMQAAQVV
CCCCCCEEEEECCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
HLTRQMSGQGITVPAAMDWPALIRSKSAFSNGVPERTERAFQQAGIKMYFGTAHFLSPET
HHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEEEECCCCE
VAIGSETTVRAETIVIATGARPAPLDFPGAGLVVETSDFMEMKNLPRRVLFIGGGCLALS
EEECCCCEEEEEEEEEECCCCCCCCCCCCCCEEEECHHHHHHHCCCCEEEEECCCHHHHH
FGHVARAAGADVTILQRGERVLKNFDLEMAQLAAKAARARGINIVTGITAAMAEKVQGAF
HHHHHHHCCCCEEEHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCHH
MTYGKGGCTEAFPSDLIVNTSGRIPDLDPVDPEAGAVARSARGVTVNEFLQSVSNPRVWA
EECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHCCCCEEE
IGDACDSPYLLSTVADMEAEVAADNILTGNRRRPDYQGVPSMAQAQPPLSFVGLTEAQAR
ECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCEEEECCHHHHH
QSGKKFRINRGSTDSWPSSRRIGQQGGFYKVLIEEETGKILGAHLLGQNAGETINIFALA
HCCCEEEEECCCCCCCCCHHHHCCCCCEEEEEEECCCCCEEEHHHHCCCCCCEEEEEEEE
LKFGISNSELRQILWTYPTFISDVKDMIE
EHHCCCHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3037534 [H]