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The map label for this gene is mycA [H]

Identifier: 197120099

GI number: 197120099

Start: 4264441

End: 4266000

Strand: Direct

Name: mycA [H]

Synonym: Gbem_3738

Alternate gene names: 197120099

Gene position: 4264441-4266000 (Clockwise)

Preceding gene: 197120098

Following gene: 197120100

Centisome position: 92.4

GC content: 62.44

Gene sequence:

>1560_bases
ATGGACGCAGCAGCCTCACACGGAACCACGGTTCACCAATATATGGAGTATTTCGTGCGGCAAGACCCCGACGCGCTCTG
CTGTTCCTTGCGCGTCAAAGGGGCCTGGGCAGATTACTCACGCCTCGAATTCTGGGACCACGTCTGCCGGTATGCCACTC
TCTTCTTAGATCTAGAGCACCAGAGCGTCATCCTCTTCGCCAAGAAGCTCGACATCCACCTGCTGACCGCCTACATCGGA
GCCATGAAGGCGGGGCATCTTCCGGCGCAGGTCAGTTTCCCGTCGGCAAAAGTCAACGAAACCGAATATCGGAAGAAGAT
CGACCACATCAGGGAGATCACCCGGTTCGGGGCGGTCTTCACCGAGGCGGAGGAAAATCACCTGCAGAAGTACCTCGGCT
CGGAGCTGGTATTTACCCCCGCCTGCGAACCGGCCGAAGGGCGCGCCCTCCCCGCAGCGGCGGCAAACCCCGAGAACGCG
CTGATCCAGTTCTCCTCCGGTTCCACGGGGCTGCAAAAGGGTGTGATCCTCACCCACGAGGCGGTGGTCGAGCACATGCA
AAGCTACGCCTCCAGCCTGAGGGTCGGGAAGGGGGACGCCATCGCCACCTGGCTTCCCCTCTACCACGACATGGGGCTCA
TCGCCTGCTACCTGATGCCGCTCATGACCGGGATCCCCTTCTATCAGCTCGACCCCTTCGACTGGATCATGCAGCCGGAC
CTCCTGCTCCAGTTGATCGAGGAGAGAAGGCCCACCATCTGCTACCTCCCGAACTTCACCTACCAGGTGCTGGCCAATAA
GGGGAAGGTGCGGGATCTCTCCAGCGTCAGGCTCTGGATCAACTGCTCGGAGCCGGCGCGGGCCAAGAGCCACCAGATGT
TCGCCCAACGCTTCCCAAGCGTGAGGCGCGACGCGCTCACCGTCTGCTACGCCCTTGCCGAGAACACCTTCGCCGCGACG
CAGACGCTCCCCTGGGAGGGGAATTCGACCAAGGTGCATCCCACCCGCAACGTCCTCTCCTGCGGCAAGCCGATCCCCGG
CGTCGAGGTGCGCATCTTCGATGCGATGGAGAACGGCGACGGCGAGATGGGGCTGCGCTCGCCGTTTCTCTACCACCGCT
TCCTGGACGGGACGCGGCCGCTCAGGGACGGCTTCTGCCTGACCGGTGACCTGGGCTTCATCGGGGAAGACGGCGAGGTC
TACATCACCGGGCGCAAGAAGGACATCATCATCGTGCACGGCAAGAACATCTATCCGCAGGACGTCGAGTACGTCGCCTC
GGAGGCCCCGGGCGCATACTCCGGGCGCACCGTCTGCTTCGGGATCTGGGACGACGAGATCGGGAGCGAGGAGCTCTACG
TCATCGTGGAACGCGAGAGCACTGCGAACCCGACCGCCATCAAGATCGCCGTGCAGAAAGCGGTCATGGAGGAGGTGGGG
ACGGTGCCGAAGAGGGTCGAGGTGGTGGAGCATATGACGCTGGTCAAGACCTCGAGCGGCAAGATCTCCCGCTCCCGCAA
CAAGGAACTCTACCAGGGCAAAGGTTTCCAACTTCTATGA

Upstream 100 bases:

>100_bases
TGCAACCCAGGGAACGAGGCGAACGCGCAACCCCTTATACGAAGACTCGAGGGCTTCCTCAGCTGACAAAGGAACTTGCC
TCACGCCTAAAGGAGACGGT

Downstream 100 bases:

>100_bases
TCATCATCGGCAACTCCAACGTGAACACCTTCAAACAGCAGGGGGTCGTCGCCGCGACCGACGCCGAGAAGGTCTCGGTG
CTCTGGGTGGGCGCCCTTAC

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Glutamate-1-semialdehyde aminotransferase; GSA-AT; ATP-dependent asparagine adenylase 1; AsnA 1; Asparagine activase 1 [H]

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MDAAASHGTTVHQYMEYFVRQDPDALCCSLRVKGAWADYSRLEFWDHVCRYATLFLDLEHQSVILFAKKLDIHLLTAYIG
AMKAGHLPAQVSFPSAKVNETEYRKKIDHIREITRFGAVFTEAEENHLQKYLGSELVFTPACEPAEGRALPAAAANPENA
LIQFSSGSTGLQKGVILTHEAVVEHMQSYASSLRVGKGDAIATWLPLYHDMGLIACYLMPLMTGIPFYQLDPFDWIMQPD
LLLQLIEERRPTICYLPNFTYQVLANKGKVRDLSSVRLWINCSEPARAKSHQMFAQRFPSVRRDALTVCYALAENTFAAT
QTLPWEGNSTKVHPTRNVLSCGKPIPGVEVRIFDAMENGDGEMGLRSPFLYHRFLDGTRPLRDGFCLTGDLGFIGEDGEV
YITGRKKDIIIVHGKNIYPQDVEYVASEAPGAYSGRTVCFGIWDDEIGSEELYVIVERESTANPTAIKIAVQKAVMEEVG
TVPKRVEVVEHMTLVKTSSGKISRSRNKELYQGKGFQLL

Sequences:

>Translated_519_residues
MDAAASHGTTVHQYMEYFVRQDPDALCCSLRVKGAWADYSRLEFWDHVCRYATLFLDLEHQSVILFAKKLDIHLLTAYIG
AMKAGHLPAQVSFPSAKVNETEYRKKIDHIREITRFGAVFTEAEENHLQKYLGSELVFTPACEPAEGRALPAAAANPENA
LIQFSSGSTGLQKGVILTHEAVVEHMQSYASSLRVGKGDAIATWLPLYHDMGLIACYLMPLMTGIPFYQLDPFDWIMQPD
LLLQLIEERRPTICYLPNFTYQVLANKGKVRDLSSVRLWINCSEPARAKSHQMFAQRFPSVRRDALTVCYALAENTFAAT
QTLPWEGNSTKVHPTRNVLSCGKPIPGVEVRIFDAMENGDGEMGLRSPFLYHRFLDGTRPLRDGFCLTGDLGFIGEDGEV
YITGRKKDIIIVHGKNIYPQDVEYVASEAPGAYSGRTVCFGIWDDEIGSEELYVIVERESTANPTAIKIAVQKAVMEEVG
TVPKRVEVVEHMTLVKTSSGKISRSRNKELYQGKGFQLL
>Mature_519_residues
MDAAASHGTTVHQYMEYFVRQDPDALCCSLRVKGAWADYSRLEFWDHVCRYATLFLDLEHQSVILFAKKLDIHLLTAYIG
AMKAGHLPAQVSFPSAKVNETEYRKKIDHIREITRFGAVFTEAEENHLQKYLGSELVFTPACEPAEGRALPAAAANPENA
LIQFSSGSTGLQKGVILTHEAVVEHMQSYASSLRVGKGDAIATWLPLYHDMGLIACYLMPLMTGIPFYQLDPFDWIMQPD
LLLQLIEERRPTICYLPNFTYQVLANKGKVRDLSSVRLWINCSEPARAKSHQMFAQRFPSVRRDALTVCYALAENTFAAT
QTLPWEGNSTKVHPTRNVLSCGKPIPGVEVRIFDAMENGDGEMGLRSPFLYHRFLDGTRPLRDGFCLTGDLGFIGEDGEV
YITGRKKDIIIVHGKNIYPQDVEYVASEAPGAYSGRTVCFGIWDDEIGSEELYVIVERESTANPTAIKIAVQKAVMEEVG
TVPKRVEVVEHMTLVKTSSGKISRSRNKELYQGKGFQLL

Specific function: This protein is a multifunctional enzyme, able to activate a long chain fatty acid and link it with the amino acid Asn as part of the synthesis of mycosubtilin. The activation sites consist of individual domains [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 acyl carrier domains [H]

Homologues:

Organism=Escherichia coli, GI1788107, Length=374, Percent_Identity=26.7379679144385, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI145693145, Length=363, Percent_Identity=26.7217630853994, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1789201, Length=361, Percent_Identity=22.7146814404432, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17558820, Length=270, Percent_Identity=28.1481481481481, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17538037, Length=386, Percent_Identity=24.3523316062176, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI32563687, Length=345, Percent_Identity=24.9275362318841, Blast_Score=78, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17557194, Length=396, Percent_Identity=25, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17560308, Length=361, Percent_Identity=25.4847645429363, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21356441, Length=374, Percent_Identity=24.331550802139, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI18859661, Length=383, Percent_Identity=25.3263707571802, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24648253, Length=394, Percent_Identity=24.1116751269036, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24648255, Length=397, Percent_Identity=24.1813602015113, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24581924, Length=388, Percent_Identity=26.2886597938144, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281365686, Length=373, Percent_Identity=23.8605898123324, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648257, Length=372, Percent_Identity=25, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR005814
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR023213
- InterPro:   IPR001242
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR006162
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00202 Aminotran_3; PF00501 AMP-binding; PF00668 Condensation; PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C; PF00550 PP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 58114; Mature: 58114

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAAASHGTTVHQYMEYFVRQDPDALCCSLRVKGAWADYSRLEFWDHVCRYATLFLDLEH
CCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHEEEECC
QSVILFAKKLDIHLLTAYIGAMKAGHLPAQVSFPSAKVNETEYRKKIDHIREITRFGAVF
CEEEEEEECCCHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
TEAEENHLQKYLGSELVFTPACEPAEGRALPAAAANPENALIQFSSGSTGLQKGVILTHE
HHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEHH
AVVEHMQSYASSLRVGKGDAIATWLPLYHDMGLIACYLMPLMTGIPFYQLDPFDWIMQPD
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHCCHH
LLLQLIEERRPTICYLPNFTYQVLANKGKVRDLSSVRLWINCSEPARAKSHQMFAQRFPS
HHHHHHHHCCCCEEECCCCEEEEECCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCH
VRRDALTVCYALAENTFAATQTLPWEGNSTKVHPTRNVLSCGKPIPGVEVRIFDAMENGD
HHHHHHHHHHHHHCCCEECCEECCCCCCCCEECCCHHHHHCCCCCCCCEEEEEEEEECCC
GEMGLRSPFLYHRFLDGTRPLRDGFCLTGDLGFIGEDGEVYITGRKKDIIIVHGKNIYPQ
CCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCEECCCCEEEEEECCCEEEEEECCCCCCC
DVEYVASEAPGAYSGRTVCFGIWDDEIGSEELYVIVERESTANPTAIKIAVQKAVMEEVG
HHHHHHHCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCEEEHHHHHHHHHHHC
TVPKRVEVVEHMTLVKTSSGKISRSRNKELYQGKGFQLL
CCHHHHHHHHHHHEEECCCCCCCCCCCCHHHCCCCCCCC
>Mature Secondary Structure
MDAAASHGTTVHQYMEYFVRQDPDALCCSLRVKGAWADYSRLEFWDHVCRYATLFLDLEH
CCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHEEEECC
QSVILFAKKLDIHLLTAYIGAMKAGHLPAQVSFPSAKVNETEYRKKIDHIREITRFGAVF
CEEEEEEECCCHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
TEAEENHLQKYLGSELVFTPACEPAEGRALPAAAANPENALIQFSSGSTGLQKGVILTHE
HHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEHH
AVVEHMQSYASSLRVGKGDAIATWLPLYHDMGLIACYLMPLMTGIPFYQLDPFDWIMQPD
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHCCHH
LLLQLIEERRPTICYLPNFTYQVLANKGKVRDLSSVRLWINCSEPARAKSHQMFAQRFPS
HHHHHHHHCCCCEEECCCCEEEEECCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCH
VRRDALTVCYALAENTFAATQTLPWEGNSTKVHPTRNVLSCGKPIPGVEVRIFDAMENGD
HHHHHHHHHHHHHCCCEECCEECCCCCCCCEECCCHHHHHCCCCCCCCEEEEEEEEECCC
GEMGLRSPFLYHRFLDGTRPLRDGFCLTGDLGFIGEDGEVYITGRKKDIIIVHGKNIYPQ
CCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCEECCCCEEEEEECCCEEEEEECCCCCCC
DVEYVASEAPGAYSGRTVCFGIWDDEIGSEELYVIVERESTANPTAIKIAVQKAVMEEVG
HHHHHHHCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCEEEHHHHHHHHHHHC
TVPKRVEVVEHMTLVKTSSGKISRSRNKELYQGKGFQLL
CCHHHHHHHHHHHEEECCCCCCCCCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10557314 [H]