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The map label for this gene is pinE [C]

Identifier: 197119372

GI number: 197119372

Start: 3468214

End: 3468807

Strand: Direct

Name: pinE [C]

Synonym: Gbem_3000

Alternate gene names: 197119372

Gene position: 3468214-3468807 (Clockwise)

Preceding gene: 197119371

Following gene: 197119387

Centisome position: 75.15

GC content: 58.08

Gene sequence:

>594_bases
ATGACCCCTAAACTATATATAGCCTACTACCGCGTATCCACCGACAAGCAGGGCGAACGCGGCCTCGGCATCCAGGCGCA
GAGGGATGCTGTCACGAGGTTCATCGAGCGCCAGGAAGGGGCTGTGCTTTACGCCTGTTTCACCGAGGTCGAATCAGGCA
CCAAGAAAGGCAACCGCCCAGGTCTTGCCGACGCTCTAGCTTTGGTCAGGAAGGTCAAAGGCACGCTCCTCATAGCCAAG
CTCGACCGTCTGGCCAGGAACGTCCGGTTCATCGCAACGCTGATGGAGGAGAACCCCGACTTCATCGCCCTGGACTTTCC
CGAGTCACAGCCGGTCATCCTCCACGTCATGGCCAGCTTCGCCGAGCACGAGGCGCGGGTGATCGCCGCCCGGACCTCAG
CCGCGATCCAGGCCCGACTCAAAGACCCCGACTACCGCAACTCCAAAGGTGCAAGATGGGGCAATCCCCAGTGCCTGAAC
CCCAAGTACAAGTCGCTGTCGAAGAGTGAAGCAGCAGCCGCACGTAGAAAAGACGCAGTGGACTTTGCGAAAAAACTGGA
GCCAATTATTAAATTTTACTTGCAGGACCGTTAA

Upstream 100 bases:

>100_bases
ACGAGAAGGAATGGGAATATCAACAGGAGATAAGGGAACATGAACGGCAGTCGAGCGAGAGGATGCACGCGGTATTCGAC
GCGAAGCGGAGGGCAAGAAG

Downstream 100 bases:

>100_bases
AGCTTAAATCTGGTGATCGGTCGGGTTCTCCAGCGGCAGACTGGCGCGGAAGGTGATTAAGTCACCTTCTTTTATTTCAG
GTTCCTGCATGGCTTACCTC

Product: resolvase-like serine recombinase

Products: NA

Alternate protein names: Resolvase Domain Protein; Site-Specific Recombinase; Resolvase; Resolvase-Like Serine Recombinase; Resolvase-Like Protein; Resolvase Family Protein; Resolvase Domain; DNA Invertase; Resolvase Family Site-Specific Recombinase; Resolvase N-Terminal; Phage Resolvase/Recombinase For Integration And Excision; DNA-Invertase; Resolvase N Terminal Domain Family; Serine Recombinase

Number of amino acids: Translated: 197; Mature: 196

Protein sequence:

>197_residues
MTPKLYIAYYRVSTDKQGERGLGIQAQRDAVTRFIERQEGAVLYACFTEVESGTKKGNRPGLADALALVRKVKGTLLIAK
LDRLARNVRFIATLMEENPDFIALDFPESQPVILHVMASFAEHEARVIAARTSAAIQARLKDPDYRNSKGARWGNPQCLN
PKYKSLSKSEAAAARRKDAVDFAKKLEPIIKFYLQDR

Sequences:

>Translated_197_residues
MTPKLYIAYYRVSTDKQGERGLGIQAQRDAVTRFIERQEGAVLYACFTEVESGTKKGNRPGLADALALVRKVKGTLLIAK
LDRLARNVRFIATLMEENPDFIALDFPESQPVILHVMASFAEHEARVIAARTSAAIQARLKDPDYRNSKGARWGNPQCLN
PKYKSLSKSEAAAARRKDAVDFAKKLEPIIKFYLQDR
>Mature_196_residues
TPKLYIAYYRVSTDKQGERGLGIQAQRDAVTRFIERQEGAVLYACFTEVESGTKKGNRPGLADALALVRKVKGTLLIAKL
DRLARNVRFIATLMEENPDFIALDFPESQPVILHVMASFAEHEARVIAARTSAAIQARLKDPDYRNSKGARWGNPQCLNP
KYKSLSKSEAAAARRKDAVDFAKKLEPIIKFYLQDR

Specific function: This Protein Catalyzes The Inversion Of An 1800-Bp E.Coli DNA Fragment, The P Region, Which Can Exist In Either Orientation. The Function Of The Inversion Is Not Yet Clear. [C]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 22114; Mature: 21983

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPKLYIAYYRVSTDKQGERGLGIQAQRDAVTRFIERQEGAVLYACFTEVESGTKKGNRP
CCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCCC
GLADALALVRKVKGTLLIAKLDRLARNVRFIATLMEENPDFIALDFPESQPVILHVMASF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHH
AEHEARVIAARTSAAIQARLKDPDYRNSKGARWGNPQCLNPKYKSLSKSEAAAARRKDAV
HHHHHHEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHH
DFAKKLEPIIKFYLQDR
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TPKLYIAYYRVSTDKQGERGLGIQAQRDAVTRFIERQEGAVLYACFTEVESGTKKGNRP
CCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCCC
GLADALALVRKVKGTLLIAKLDRLARNVRFIATLMEENPDFIALDFPESQPVILHVMASF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHH
AEHEARVIAARTSAAIQARLKDPDYRNSKGARWGNPQCLNPKYKSLSKSEAAAARRKDAV
HHHHHHEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHH
DFAKKLEPIIKFYLQDR
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA