Click here to switch to the map view.

The map label for this gene is aspA [H]

Identifier: 197119168

GI number: 197119168

Start: 3222525

End: 3223925

Strand: Direct

Name: aspA [H]

Synonym: Gbem_2795

Alternate gene names: 197119168

Gene position: 3222525-3223925 (Clockwise)

Preceding gene: 197119167

Following gene: 197119169

Centisome position: 69.82

GC content: 64.03

Gene sequence:

>1401_bases
ATGGCAACACGACTCGAAAAAGACACTATGGGTGTGGTTGAGGTACCGGAGGGGGCCTATTACGGGGCTCAGACCCAGAG
GGCCGTCGCCAACTTCCCCATTTCAGGACTAAAACCGCACCATGCCCTGGTCCGATCCACGGTGAGGATAAAGAAGTGCG
CCGCGCAGGCGAACATGACGACCGGCAGGCTCGACGCCGAGGTGGGAGGCGCCATCGTGAAGGCGGCCGATGAGGTTCTG
TCCGGAGCGCTCGCCGACCAGTTCGTGGTGGACCCGTTCCAGGCCGGCGCGGGGACCTCGCACAACATGAACGTCAACGA
GGTGTTGGCCAACCGCGCGGCGGAACTGTTGGGCGGCAAGCAGGGCGACTATTCGCGAGTCAATCCCAACGACCATGTCA
ACATGGCCCAGTCCACCAACGACGTCTTCCCGACCGCGATGCGGCTCGCCGCGCTGGAATTGGCAGAGCAGACGATGACG
GAGCTGGCGGGCCTTTCCGCAGCCTTCGAACAAAAGGGTAAGGAGTTCGACGGGATACTGAAGTCCGGGCGGACCCACCT
GCAGGACGCAGTCCCCATCCGTCTGGGGCAAGAGTTCGCCGCCTACGGCAAGGCCATCGGGAACAATCGCGCCGGCATCG
AGCGGGCGCTTCCCGCCCTGAGGGAGCTGGGCATCGGCGGGACTGCCGTCGGCACGGGGCTCAATGCGGAAGAGGCCTTC
ATCGACCTGATAGTGCAGGGGCTCGCCCGCGAAACCGGACAGGAAGTCAATCGTGGGGGAAACCTGGTCGAGCGGATGCA
GAACATGGATCCCTTCGTGGCGCTCAGTTCCACCCTCAAGGGGACAGCTCTCAACCTGATCCGGATCGCCAACGACTTGA
GGCTTCTCTCCTCGGGGCCCAGGACCGGTTTGGGCGAGATCAACCTCCCCGCGATGCAGCCCGGCTCCTCCATCATGCCG
GGGAAGGTGAATCCGGTCATGCCGGAGGTGACCACCATGGTCTGCTTCCAGGTGGTCGGCGCGGATCTTACCGTCGCCAT
GGCGGCGCAGGCCGGGCAGTTGGAGCTGAACGTCATGATGCCGGTCATCGCCTTCAACACGCTCTTCTCCCTGGAGATAC
TGAAGAACGTGGTGCATCAGTTCACCACCTTGTGCGTCGCCGGCATCAGCGCCAACGAGGAGCACTGCCGCAACTACCTG
GACCAGTCGGTCGGGCTTGCCACCGTGCTCGCGCCGAGCATCGGCTACGCCGCGGCGGCAGAGGTCGCCAAGGAGTCGGC
CCGGACCGGGAAGAGCATTAGGCAGGTGATACTGGAAAGCGGCATACTGACCGAGGCGGAACTGGACCAGGTGCTGGCCC
CCTTCCCGCTCACTACTCCGGGCGTGCATGGCAAGGGTTGA

Upstream 100 bases:

>100_bases
ACGCTATAAGGGAAGCTGCAAAGTCTATATTCGAATTCACCTGAAACAGCCCGAGCTTTATTTGATTCTGGACACTGGCA
GAAGCTGGGAAGGAGCAGAC

Downstream 100 bases:

>100_bases
CCTTAGTCCCCCCCTCCCTTGACGGGCCTGCGCGCCGGAGCGCTACGGCTCGCGAAGGCGGGAGGGGGTCGGGGGGTGGG
TGAAGCTGCCAACGTAGTCG

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MATRLEKDTMGVVEVPEGAYYGAQTQRAVANFPISGLKPHHALVRSTVRIKKCAAQANMTTGRLDAEVGGAIVKAADEVL
SGALADQFVVDPFQAGAGTSHNMNVNEVLANRAAELLGGKQGDYSRVNPNDHVNMAQSTNDVFPTAMRLAALELAEQTMT
ELAGLSAAFEQKGKEFDGILKSGRTHLQDAVPIRLGQEFAAYGKAIGNNRAGIERALPALRELGIGGTAVGTGLNAEEAF
IDLIVQGLARETGQEVNRGGNLVERMQNMDPFVALSSTLKGTALNLIRIANDLRLLSSGPRTGLGEINLPAMQPGSSIMP
GKVNPVMPEVTTMVCFQVVGADLTVAMAAQAGQLELNVMMPVIAFNTLFSLEILKNVVHQFTTLCVAGISANEEHCRNYL
DQSVGLATVLAPSIGYAAAAEVAKESARTGKSIRQVILESGILTEAELDQVLAPFPLTTPGVHGKG

Sequences:

>Translated_466_residues
MATRLEKDTMGVVEVPEGAYYGAQTQRAVANFPISGLKPHHALVRSTVRIKKCAAQANMTTGRLDAEVGGAIVKAADEVL
SGALADQFVVDPFQAGAGTSHNMNVNEVLANRAAELLGGKQGDYSRVNPNDHVNMAQSTNDVFPTAMRLAALELAEQTMT
ELAGLSAAFEQKGKEFDGILKSGRTHLQDAVPIRLGQEFAAYGKAIGNNRAGIERALPALRELGIGGTAVGTGLNAEEAF
IDLIVQGLARETGQEVNRGGNLVERMQNMDPFVALSSTLKGTALNLIRIANDLRLLSSGPRTGLGEINLPAMQPGSSIMP
GKVNPVMPEVTTMVCFQVVGADLTVAMAAQAGQLELNVMMPVIAFNTLFSLEILKNVVHQFTTLCVAGISANEEHCRNYL
DQSVGLATVLAPSIGYAAAAEVAKESARTGKSIRQVILESGILTEAELDQVLAPFPLTTPGVHGKG
>Mature_465_residues
ATRLEKDTMGVVEVPEGAYYGAQTQRAVANFPISGLKPHHALVRSTVRIKKCAAQANMTTGRLDAEVGGAIVKAADEVLS
GALADQFVVDPFQAGAGTSHNMNVNEVLANRAAELLGGKQGDYSRVNPNDHVNMAQSTNDVFPTAMRLAALELAEQTMTE
LAGLSAAFEQKGKEFDGILKSGRTHLQDAVPIRLGQEFAAYGKAIGNNRAGIERALPALRELGIGGTAVGTGLNAEEAFI
DLIVQGLARETGQEVNRGGNLVERMQNMDPFVALSSTLKGTALNLIRIANDLRLLSSGPRTGLGEINLPAMQPGSSIMPG
KVNPVMPEVTTMVCFQVVGADLTVAMAAQAGQLELNVMMPVIAFNTLFSLEILKNVVHQFTTLCVAGISANEEHCRNYLD
QSVGLATVLAPSIGYAAAAEVAKESARTGKSIRQVILESGILTEAELDQVLAPFPLTTPGVHGKG

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=460, Percent_Identity=44.1304347826087, Blast_Score=365, Evalue=1e-101,
Organism=Escherichia coli, GI87082375, Length=465, Percent_Identity=47.9569892473118, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1787896, Length=452, Percent_Identity=44.4690265486726, Blast_Score=370, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17553882, Length=454, Percent_Identity=44.0528634361233, Blast_Score=363, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32565146, Length=321, Percent_Identity=42.6791277258567, Blast_Score=253, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6324993, Length=460, Percent_Identity=44.7826086956522, Blast_Score=378, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24640179, Length=464, Percent_Identity=43.75, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24640177, Length=464, Percent_Identity=43.75, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24662684, Length=463, Percent_Identity=41.4686825053996, Blast_Score=348, Evalue=4e-96,
Organism=Drosophila melanogaster, GI78710009, Length=462, Percent_Identity=40.9090909090909, Blast_Score=341, Evalue=7e-94,
Organism=Drosophila melanogaster, GI24583245, Length=448, Percent_Identity=39.5089285714286, Blast_Score=324, Evalue=1e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 49106; Mature: 48975

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRLEKDTMGVVEVPEGAYYGAQTQRAVANFPISGLKPHHALVRSTVRIKKCAAQANMT
CCCCCCCCCCCEEECCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
TGRLDAEVGGAIVKAADEVLSGALADQFVVDPFQAGAGTSHNMNVNEVLANRAAELLGGK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHHHCCC
QGDYSRVNPNDHVNMAQSTNDVFPTAMRLAALELAEQTMTELAGLSAAFEQKGKEFDGIL
CCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
KSGRTHLQDAVPIRLGQEFAAYGKAIGNNRAGIERALPALRELGIGGTAVGTGLNAEEAF
HCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHCCCCCHHHHH
IDLIVQGLARETGQEVNRGGNLVERMQNMDPFVALSSTLKGTALNLIRIANDLRLLSSGP
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCC
RTGLGEINLPAMQPGSSIMPGKVNPVMPEVTTMVCFQVVGADLTVAMAAQAGQLELNVMM
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCEEEHHHH
PVIAFNTLFSLEILKNVVHQFTTLCVAGISANEEHCRNYLDQSVGLATVLAPSIGYAAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHCCCCHHHHH
EVAKESARTGKSIRQVILESGILTEAELDQVLAPFPLTTPGVHGKG
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
ATRLEKDTMGVVEVPEGAYYGAQTQRAVANFPISGLKPHHALVRSTVRIKKCAAQANMT
CCCCCCCCCCEEECCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
TGRLDAEVGGAIVKAADEVLSGALADQFVVDPFQAGAGTSHNMNVNEVLANRAAELLGGK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHHHCCC
QGDYSRVNPNDHVNMAQSTNDVFPTAMRLAALELAEQTMTELAGLSAAFEQKGKEFDGIL
CCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
KSGRTHLQDAVPIRLGQEFAAYGKAIGNNRAGIERALPALRELGIGGTAVGTGLNAEEAF
HCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHCCCCCHHHHH
IDLIVQGLARETGQEVNRGGNLVERMQNMDPFVALSSTLKGTALNLIRIANDLRLLSSGP
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCC
RTGLGEINLPAMQPGSSIMPGKVNPVMPEVTTMVCFQVVGADLTVAMAAQAGQLELNVMM
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCEEEHHHH
PVIAFNTLFSLEILKNVVHQFTTLCVAGISANEEHCRNYLDQSVGLATVLAPSIGYAAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHCCCCHHHHH
EVAKESARTGKSIRQVILESGILTEAELDQVLAPFPLTTPGVHGKG
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]