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The map label for this gene is trmJ [H]

Identifier: 197119062

GI number: 197119062

Start: 3109875

End: 3110612

Strand: Reverse

Name: trmJ [H]

Synonym: Gbem_2687

Alternate gene names: 197119062

Gene position: 3110612-3109875 (Counterclockwise)

Preceding gene: 197119063

Following gene: 197119061

Centisome position: 67.4

GC content: 62.06

Gene sequence:

>738_bases
ATGGATTTGAAGCAAAGAGTATCCGTCGTTTTGGTGGGGACCCAGAGTCCCGGTAATATAGGCATGGTCTGCAGGGCTAT
GAAGAACATGGGGCTCTCCGACCTGCGCCTGGTAAACCCCTGCCAGGTCGATCATCTGGACGCGGTCAAGTTCGCCGTTT
CCGCACGCAATCTCCTCGACTCTGCCCGGATCTTCACCTCCCTCGAAGACGCCATCGCCGACTGCGAGTTCTCCGTTGCC
ACCACCAGGCGCCACGGCAAATACCGCAACGAGATCTCCACCCCGGCGGAGATCGTGGAGCGCTTCTCCGCCTGTGCGCC
GGCAAGCCGCATGGCGCTGGTCTTCGGGCGCGAGGACAGCGGCCTCACCACCGACGAAGTCGCCCTTTGCCGCTGGCAAG
CCACCATCGAGACCGCCGACGAATTCGGTTCCCTGAACCTCTCCCAAGCGGTCCTCATCTTCTGCTACGAGTTTCTCAAA
GGCGTAGCACCCGCGCCGGTGAAGCAGGCCCGCGACATTGCCGGGTCTGCCTCCCTTGAGCCGCTGTACCAGCACATGGA
GCGCTCCTTCCTGAGGATCGGCTACCTCAACCCGCAAAACCCCGATCACATGATGCGGACCCTGCGTCGCATTTTCTCCC
GCGCCGAACTAGACGAGCGCGAGGTCTCCTCGCTGCGCGGGCTCCTCTCCCAGATAGACTGGGCCTGCTCCGAGTTCAAA
GGGAAGAAGGGCGAATGA

Upstream 100 bases:

>100_bases
CTTTGACTCTGCAGTTGAAAACCCCGCCCTCTTGTGGTCTAATGCAGCTCCTGGTGTTCGCCTTCCCAGTTTTAACAATT
CCCACTGACAAAGGTTCTGC

Downstream 100 bases:

>100_bases
CCCTCGTCGGCACCTTGGGCTTTTGCGCCGGGCTCCTCACCAGCGGGGCGGCGGTGCCCCAGGTCTACAAGACCTACAAG
ACCAGGCACGCGCGCGACAT

Product: tRNA (2'O-methyl-C32/U32)-methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MDLKQRVSVVLVGTQSPGNIGMVCRAMKNMGLSDLRLVNPCQVDHLDAVKFAVSARNLLDSARIFTSLEDAIADCEFSVA
TTRRHGKYRNEISTPAEIVERFSACAPASRMALVFGREDSGLTTDEVALCRWQATIETADEFGSLNLSQAVLIFCYEFLK
GVAPAPVKQARDIAGSASLEPLYQHMERSFLRIGYLNPQNPDHMMRTLRRIFSRAELDEREVSSLRGLLSQIDWACSEFK
GKKGE

Sequences:

>Translated_245_residues
MDLKQRVSVVLVGTQSPGNIGMVCRAMKNMGLSDLRLVNPCQVDHLDAVKFAVSARNLLDSARIFTSLEDAIADCEFSVA
TTRRHGKYRNEISTPAEIVERFSACAPASRMALVFGREDSGLTTDEVALCRWQATIETADEFGSLNLSQAVLIFCYEFLK
GVAPAPVKQARDIAGSASLEPLYQHMERSFLRIGYLNPQNPDHMMRTLRRIFSRAELDEREVSSLRGLLSQIDWACSEFK
GKKGE
>Mature_245_residues
MDLKQRVSVVLVGTQSPGNIGMVCRAMKNMGLSDLRLVNPCQVDHLDAVKFAVSARNLLDSARIFTSLEDAIADCEFSVA
TTRRHGKYRNEISTPAEIVERFSACAPASRMALVFGREDSGLTTDEVALCRWQATIETADEFGSLNLSQAVLIFCYEFLK
GVAPAPVKQARDIAGSASLEPLYQHMERSFLRIGYLNPQNPDHMMRTLRRIFSRAELDEREVSSLRGLLSQIDWACSEFK
GKKGE

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=248, Percent_Identity=34.2741935483871, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1790865, Length=156, Percent_Identity=37.8205128205128, Blast_Score=108, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27266; Mature: 27266

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLKQRVSVVLVGTQSPGNIGMVCRAMKNMGLSDLRLVNPCQVDHLDAVKFAVSARNLLD
CCHHHCEEEEEEECCCCCCHHHHHHHHHHCCCCHHEECCCCCCCHHHHHHHHHHHHHHHH
SARIFTSLEDAIADCEFSVATTRRHGKYRNEISTPAEIVERFSACAPASRMALVFGREDS
HHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHCCCHHHHHHHHHHCCCCHHEEEEEECCCC
GLTTDEVALCRWQATIETADEFGSLNLSQAVLIFCYEFLKGVAPAPVKQARDIAGSASLE
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHH
PLYQHMERSFLRIGYLNPQNPDHMMRTLRRIFSRAELDEREVSSLRGLLSQIDWACSEFK
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
GKKGE
CCCCC
>Mature Secondary Structure
MDLKQRVSVVLVGTQSPGNIGMVCRAMKNMGLSDLRLVNPCQVDHLDAVKFAVSARNLLD
CCHHHCEEEEEEECCCCCCHHHHHHHHHHCCCCHHEECCCCCCCHHHHHHHHHHHHHHHH
SARIFTSLEDAIADCEFSVATTRRHGKYRNEISTPAEIVERFSACAPASRMALVFGREDS
HHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHCCCHHHHHHHHHHCCCCHHEEEEEECCCC
GLTTDEVALCRWQATIETADEFGSLNLSQAVLIFCYEFLKGVAPAPVKQARDIAGSASLE
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHH
PLYQHMERSFLRIGYLNPQNPDHMMRTLRRIFSRAELDEREVSSLRGLLSQIDWACSEFK
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
GKKGE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA