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The map label for this gene is cheR-10 [C]

Identifier: 197118874

GI number: 197118874

Start: 2895847

End: 2896683

Strand: Reverse

Name: cheR-10 [C]

Synonym: Gbem_2497

Alternate gene names: 197118874

Gene position: 2896683-2895847 (Counterclockwise)

Preceding gene: 197118875

Following gene: 197118873

Centisome position: 62.76

GC content: 59.38

Gene sequence:

>837_bases
TTGAGTGACACAGTTGCCGAGATTCTGCGTTTACTAACGGAGCATTTTGGCGAAGACTTCCAACACTATTCGCTGACCCT
TGCGGCAAAGAGAATTGCGGAGCGGGTGGTCCAACTACGTTTGCCCGACGTCGCCGCGTACCACCGGTATTTGAACGACA
ACCCTTCAGAACTCTCCTGTCTTGCGCGCATGCTGCGCATCCGTTTCAGCAGTTTCTTCCGCGATACGCTCCAGTTCGAG
CTTCTGGGCTCGGTCATCATTCCCTCCATGCTGCCGGGAAACACCAACGGTTTTTTCCGCGCCTGGTCGGCAGCCTGCGC
CGGGGGGGAGGAGGCGTGCTCGCTTGCCATCATCATCGACGAGGCGCTGCAGCTTCTCGGCCTGAAAACGCGGGTGCAGA
TCTTCGCCACCGACATCGCCGAAGACGCGCTGGCCGAGGCCGAATACGGCAGGTACAGCACGGCCGCCGTCGCCGGGGTG
ACGCTGCAGCGGCTCAACAAGTACTTCATCTACGACCGGACCGGTTACCAGATCTCGCCCCGGATCAAGGAGATGATCAC
CTACTCGCGGCACGACCTGCTCGATCCGAACACCTACGCACCGCCTGAATCGCTTTTCGGGGGGTTCGACCTGGTCTCCT
GCCGTAACTTCCTCATGTACCTTGACTCGCCCGGGTATCTGCGGGTCTTCGACAACCTTTTCCGTGCCTTGAACCCCTAC
GGGGTGCTGCTCTTGGGAAAGGCGGAAACGGTACCTGACCGGTACGAACCGTACCTGGAGCGGATGTTCGATTGCGGCAA
CCTGTTCCGCAAAAGACAGATGGTGAGGAGAAGCTGA

Upstream 100 bases:

>100_bases
TTGGATGATTTTAATTGTTGTGGAATGCTGCTCCACCACTGAATGTAGCTGTGCAATGGCAGTGAACCGCTCGCTTGAAA
GCAGTGTTCCGGAGGCCGCG

Downstream 100 bases:

>100_bases
TGAAAACGCCCCTGCACCTCATCATGGTACTTTTCGCCGCTTTGGCGCTTTGCGGCTGCTCCAGTCAGGCGCCCGGCTCC
GCCGGCCAAGGCGGCTCCTC

Product: chemotaxis protein-glutamate methyltransferase CheR

Products: S-adenosyl-L-homocysteine; protein L-glutamate methyl ester

Alternate protein names: ORF19 [H]

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MSDTVAEILRLLTEHFGEDFQHYSLTLAAKRIAERVVQLRLPDVAAYHRYLNDNPSELSCLARMLRIRFSSFFRDTLQFE
LLGSVIIPSMLPGNTNGFFRAWSAACAGGEEACSLAIIIDEALQLLGLKTRVQIFATDIAEDALAEAEYGRYSTAAVAGV
TLQRLNKYFIYDRTGYQISPRIKEMITYSRHDLLDPNTYAPPESLFGGFDLVSCRNFLMYLDSPGYLRVFDNLFRALNPY
GVLLLGKAETVPDRYEPYLERMFDCGNLFRKRQMVRRS

Sequences:

>Translated_278_residues
MSDTVAEILRLLTEHFGEDFQHYSLTLAAKRIAERVVQLRLPDVAAYHRYLNDNPSELSCLARMLRIRFSSFFRDTLQFE
LLGSVIIPSMLPGNTNGFFRAWSAACAGGEEACSLAIIIDEALQLLGLKTRVQIFATDIAEDALAEAEYGRYSTAAVAGV
TLQRLNKYFIYDRTGYQISPRIKEMITYSRHDLLDPNTYAPPESLFGGFDLVSCRNFLMYLDSPGYLRVFDNLFRALNPY
GVLLLGKAETVPDRYEPYLERMFDCGNLFRKRQMVRRS
>Mature_277_residues
SDTVAEILRLLTEHFGEDFQHYSLTLAAKRIAERVVQLRLPDVAAYHRYLNDNPSELSCLARMLRIRFSSFFRDTLQFEL
LGSVIIPSMLPGNTNGFFRAWSAACAGGEEACSLAIIIDEALQLLGLKTRVQIFATDIAEDALAEAEYGRYSTAAVAGVT
LQRLNKYFIYDRTGYQISPRIKEMITYSRHDLLDPNTYAPPESLFGGFDLVSCRNFLMYLDSPGYLRVFDNLFRALNPYG
VLLLGKAETVPDRYEPYLERMFDCGNLFRKRQMVRRS

Specific function: Methylation Of The Membrane-Bound Methyl-Accepting Chemotaxis Proteins (Mcp) To Form Gamma-Glutamyl Methyl Ester Residues In Mcp. [C]

COG id: COG1352

COG function: function code NT; Methylase of chemotaxis methyl-accepting proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 cheR-type methyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1788193, Length=237, Percent_Identity=27.8481012658228, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022642
- InterPro:   IPR000780
- InterPro:   IPR022641
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR000673 [H]

Pfam domain/function: PF01339 CheB_methylest; PF01739 CheR; PF03705 CheR_N; PF08448 PAS_4 [H]

EC number: 2.1.1.80

Molecular weight: Translated: 31723; Mature: 31592

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50123 CHER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTVAEILRLLTEHFGEDFQHYSLTLAAKRIAERVVQLRLPDVAAYHRYLNDNPSELSC
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHH
LARMLRIRFSSFFRDTLQFELLGSVIIPSMLPGNTNGFFRAWSAACAGGEEACSLAIIID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHH
EALQLLGLKTRVQIFATDIAEDALAEAEYGRYSTAAVAGVTLQRLNKYFIYDRTGYQISP
HHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEECCCCCEECH
RIKEMITYSRHDLLDPNTYAPPESLFGGFDLVSCRNFLMYLDSPGYLRVFDNLFRALNPY
HHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
GVLLLGKAETVPDRYEPYLERMFDCGNLFRKRQMVRRS
CEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDTVAEILRLLTEHFGEDFQHYSLTLAAKRIAERVVQLRLPDVAAYHRYLNDNPSELSC
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHH
LARMLRIRFSSFFRDTLQFELLGSVIIPSMLPGNTNGFFRAWSAACAGGEEACSLAIIID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHH
EALQLLGLKTRVQIFATDIAEDALAEAEYGRYSTAAVAGVTLQRLNKYFIYDRTGYQISP
HHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEECCCCCEECH
RIKEMITYSRHDLLDPNTYAPPESLFGGFDLVSCRNFLMYLDSPGYLRVFDNLFRALNPY
HHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
GVLLLGKAETVPDRYEPYLERMFDCGNLFRKRQMVRRS
CEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Ca2+; Mg2+ [C]

Kcat value (1/min): 10 [C]

Specific activity: NA

Km value (mM): 0.017 {S-adenosyl-L-methionine}} 0.01 {S-adenosyl-L-methionine}} [C]

Substrates: S-adenosyl-L-methionine; protein L-glutamate

Specific reaction: S-adenosyl-L-methionine + protein L-glutamate = S-adenosyl-L-homocysteine + protein L-glutamate methyl ester

General reaction: Methyl group transfer [C]

Inhibitor: A9145C; Ca2+; Methylated receptor protein; S-Adenosyl -L-homocysteine; Sinefungin [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8497190 [H]