Click here to switch to the map view.

The map label for this gene is aguB

Identifier: 197118849

GI number: 197118849

Start: 2862057

End: 2862938

Strand: Direct

Name: aguB

Synonym: Gbem_2472

Alternate gene names: NA

Gene position: 2862057-2862938 (Clockwise)

Preceding gene: 197118848

Following gene: 308535304

Centisome position: 62.01

GC content: 64.85

Gene sequence:

>882_bases
ATGCAGAAACTGAAAGTAGCTCTGGTCCAGCAGGCGCTCCGCCCGGACCGCGAGAAGATGGTAGCCGCCACCTCCGCCCA
GATCCGCGAGGCTGCAGCCCAAGGGGCAAAACTGGTGCTGCTGCAGGAACTCCACACCGGCAGTTATTTCTGCCAGACCG
AGGATACCGCCTGCTTCGACCTGGCCGAGTCCATACCCGGCCCCTCCACCGACCATTTCGGGGCCTTGGCCCGCGAGCTT
GGCGTGGTGATCGTCACCTCCCTGTTCGAAAAACGCGCCCCCGGGCTTTACCACAACACCGCAGTCGTGATCGAGAAGGA
CGGGAGCATCGCCGGGAAGTACCGCAAGATGCACATCCCCGATGACCCGGCGTTCTACGAGAAGTTCTACTTCACCCCCG
GCGATCTCGGCTTCGAGCCGGTGCAGACCTCGGTGGGCAAGCTCGGCGTCTTGGTCTGCTGGGACCAGTGGTACCCGGAA
GCGGCGAGGCTTATGGCCTTGGCCGGTGCGGATCTCCTTATCTACCCCACCGCCATCGGCTGGGACCCGAGGGACGAGGA
GGCCGAGCAGCAAAGGCAGCTCGACGCCTGGGTCACCATCCAGCGCTCCCATGCCGTCGCAAACGGCATACCGGTAGTCA
GCGTGAACCGGGTCGGTTTCGAGGAAGACCCAAGCGGCGCCGGCGCCGGGATCAAGTTCTGGGGCTCCAGTTTCGCTGCC
GGCCCGCAAGGTGAACTGCTCGCGCGCGGCGGCGAGGAGGAAGAGCTCCTGGTCGTGGACCTGGATCTGCGCCGCAGCGA
AAACGTGCGCCGCATCTGGCCCTTCCTGCGCGACAGGAGGATCGACGCCTACCAGGACCTGGTCAAGCGCTACCGGGATT
AA

Upstream 100 bases:

>100_bases
CGGCATAGACTGCCTGCCGCTCATACTGCAGCACGGCTCGCTGCACTGCGTCACCATGCAGCTCCCCAAGGGAAGCCTGA
AGAAATAGAGGGAGATTCAC

Downstream 100 bases:

>100_bases
CCCCAAAAAGCACTCACCACTGAGGAACACAGAGGTGCACAGAGGAAAATACAAAACTGTAACTGCAAAGGCATTCCTGA
TCAGGCATTATCTTCAGCCA

Product: N-carbamoylputrescine amidohydrolase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MQKLKVALVQQALRPDREKMVAATSAQIREAAAQGAKLVLLQELHTGSYFCQTEDTACFDLAESIPGPSTDHFGALAREL
GVVIVTSLFEKRAPGLYHNTAVVIEKDGSIAGKYRKMHIPDDPAFYEKFYFTPGDLGFEPVQTSVGKLGVLVCWDQWYPE
AARLMALAGADLLIYPTAIGWDPRDEEAEQQRQLDAWVTIQRSHAVANGIPVVSVNRVGFEEDPSGAGAGIKFWGSSFAA
GPQGELLARGGEEEELLVVDLDLRRSENVRRIWPFLRDRRIDAYQDLVKRYRD

Sequences:

>Translated_293_residues
MQKLKVALVQQALRPDREKMVAATSAQIREAAAQGAKLVLLQELHTGSYFCQTEDTACFDLAESIPGPSTDHFGALAREL
GVVIVTSLFEKRAPGLYHNTAVVIEKDGSIAGKYRKMHIPDDPAFYEKFYFTPGDLGFEPVQTSVGKLGVLVCWDQWYPE
AARLMALAGADLLIYPTAIGWDPRDEEAEQQRQLDAWVTIQRSHAVANGIPVVSVNRVGFEEDPSGAGAGIKFWGSSFAA
GPQGELLARGGEEEELLVVDLDLRRSENVRRIWPFLRDRRIDAYQDLVKRYRD
>Mature_293_residues
MQKLKVALVQQALRPDREKMVAATSAQIREAAAQGAKLVLLQELHTGSYFCQTEDTACFDLAESIPGPSTDHFGALAREL
GVVIVTSLFEKRAPGLYHNTAVVIEKDGSIAGKYRKMHIPDDPAFYEKFYFTPGDLGFEPVQTSVGKLGVLVCWDQWYPE
AARLMALAGADLLIYPTAIGWDPRDEEAEQQRQLDAWVTIQRSHAVANGIPVVSVNRVGFEEDPSGAGAGIKFWGSSFAA
GPQGELLARGGEEEELLVVDLDLRRSENVRRIWPFLRDRRIDAYQDLVKRYRD

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=295, Percent_Identity=30.1694915254237, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI7706509, Length=276, Percent_Identity=31.1594202898551, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI297632348, Length=292, Percent_Identity=27.7397260273973, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI297632350, Length=292, Percent_Identity=27.7397260273973, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI5031947, Length=292, Percent_Identity=27.7397260273973, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI297632346, Length=195, Percent_Identity=30.2564102564103, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17533173, Length=303, Percent_Identity=29.7029702970297, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17556280, Length=287, Percent_Identity=25.4355400696864, Blast_Score=87, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6323383, Length=296, Percent_Identity=27.7027027027027, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6322335, Length=313, Percent_Identity=24.2811501597444, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21358471, Length=298, Percent_Identity=31.8791946308725, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17933642, Length=288, Percent_Identity=24.6527777777778, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21355835, Length=292, Percent_Identity=28.0821917808219, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 32564; Mature: 32564

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLKVALVQQALRPDREKMVAATSAQIREAAAQGAKLVLLQELHTGSYFCQTEDTACFD
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEECCCCHHHH
LAESIPGPSTDHFGALARELGVVIVTSLFEKRAPGLYHNTAVVIEKDGSIAGKYRKMHIP
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCEEEEECC
DDPAFYEKFYFTPGDLGFEPVQTSVGKLGVLVCWDQWYPEAARLMALAGADLLIYPTAIG
CCHHHHHHEECCCCCCCCCHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCCEEEEEEECC
WDPRDEEAEQQRQLDAWVTIQRSHAVANGIPVVSVNRVGFEEDPSGAGAGIKFWGSSFAA
CCCCCHHHHHHHHHHHHEEEECCHHHHCCCCEEEEECCCCCCCCCCCCCCEEECCCCCCC
GPQGELLARGGEEEELLVVDLDLRRSENVRRIWPFLRDRRIDAYQDLVKRYRD
CCCCCCEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure
MQKLKVALVQQALRPDREKMVAATSAQIREAAAQGAKLVLLQELHTGSYFCQTEDTACFD
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEECCCCHHHH
LAESIPGPSTDHFGALARELGVVIVTSLFEKRAPGLYHNTAVVIEKDGSIAGKYRKMHIP
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCEEEEECC
DDPAFYEKFYFTPGDLGFEPVQTSVGKLGVLVCWDQWYPEAARLMALAGADLLIYPTAIG
CCHHHHHHEECCCCCCCCCHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCCEEEEEEECC
WDPRDEEAEQQRQLDAWVTIQRSHAVANGIPVVSVNRVGFEEDPSGAGAGIKFWGSSFAA
CCCCCHHHHHHHHHHHHEEEECCHHHHCCCCEEEEECCCCCCCCCCCCCCEEECCCCCCC
GPQGELLARGGEEEELLVVDLDLRRSENVRRIWPFLRDRRIDAYQDLVKRYRD
CCCCCCEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]