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The map label for this gene is acrA [H]

Identifier: 197118750

GI number: 197118750

Start: 2748800

End: 2750101

Strand: Reverse

Name: acrA [H]

Synonym: Gbem_2369

Alternate gene names: 197118750

Gene position: 2750101-2748800 (Counterclockwise)

Preceding gene: 197118751

Following gene: 197118749

Centisome position: 59.59

GC content: 66.67

Gene sequence:

>1302_bases
ATGCAGAGTAAGTACAGGGCGCATTTATTTACCGTTGTTCTTTTGAGCGGCATTTTGGGGCTGACCGGCTGCGGCAAAAA
GGAGCAGTCCAAGGGGCCCCAGATGGGCCCGGCCGAGGTTGGCGTGATCGAGGTGAAGCCTGAGCGCATCGCCTTGACCA
CCGAGCTGCCCGGTCGTACCTCCCCGTACCTCATTGCTGAGGTGCGGCCGCAGGTAAGCGGCATCATCCAGAAACGCGTG
TTCACCGAGGGTAGCGACGTGAAGGCGGGGCAGGTACTCTACCAGATCGATCCCGCAACCTACCAGGCTGCCTACGCCAG
CGCCAAGGCCAGCGAGGCGCGGGCCGAGGCCAACCTCGTTCCGGCCAAACTCAAGGAAGAGCGTTTCCGCGACCTGGTCA
AGATCAACGCGGTCGCCAAGCAGGACTACGACAACGCCTACGCCGCCTTGAAGCAGGCGGAGGCGGACGTGGCTTCAGCC
AAGGCAGCCGTCGAGACCGCGAAGATCAACCTTGCCTATACCCGCGTGACCGCCCCCATCTCTGGACGCGTCGGCCGCTC
CGCAGTGACCGACGGCGCGCTGGTCACCGCGAATCAGCCCGCGGCACTGGCGACCATACAGCAGTTGGGCAGCGTGTACG
TCGACGTGACCCAGTCCAGTTCCGACATGCTGAAGCTGAACCGCAGCCTCGCCAGCGGACTCTTGAAAAGGGACGCGGCC
GGTCAGGCACGGGTCAAGCTCCTCCTTGAGGACGGCACCCCGTACCCGGTGACGGGGTCGCTTAAGTTCTCCGACGTCAC
CGTGGACCAGAGCACCGGTTCCATCACGCTGCGCGCCGTCTTCCCGAACCCGAAACAGACGCTCCTCCCTGGTATGTTCG
TGCGCGCCGTACTGGAAGAGGGGATCAACGAGGCGGCCATCCTGGTGCCGCAGCGCGGCGTCACCCGCAACGCGGCCGGA
CTGCCGATAGTGATGGTGGTAGGCGCAGACGGCACGGCGCAACCGCGCCCCATCCAGGTCGCCCGTACCGTGGGCGAAAG
CTGGCTGGTGAGCGGTGGCTTGAACCCGGGGGACAAGGTGATCCTGGAAGGGCTCCAGAAAGCCCGCCCCGGCACCCCTG
TGAAGGCGGTCCCGTTCCAGGCCCAGCCGCAGGGCGCACCCCAGGGTGCTCCGGGCGCCGCCCAAGGCGCTCCCGGTGCG
GCCAAGAGCGCACCTCAGGCAGCTGCCCCTGGGGCCGCGGCTCCCGGTGCCAAGCCGGCGGCGCAGGGCTCGCCGGCAGC
GGCACAACCACAGAAGAAGTAG

Upstream 100 bases:

>100_bases
GCGCAAATGACGATGCGACGGCTGCCCTGCGCAAGCCGGCAAAATATTGAAACGGTTCCTGAATATAACGACGGAAGTAT
CATAAACTACGAGGTGTTTC

Downstream 100 bases:

>100_bases
CAGCGGCGCGGAGCGCCAAGGGGGACAGGCACCTGCGGAGCCAGTCCCCTTAGGCGACCCCGCACGGCGCTTGAAGTTGC
ACGCAAACGAACAACGTTAA

Product: RND family efflux pump membrane fusion lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MQSKYRAHLFTVVLLSGILGLTGCGKKEQSKGPQMGPAEVGVIEVKPERIALTTELPGRTSPYLIAEVRPQVSGIIQKRV
FTEGSDVKAGQVLYQIDPATYQAAYASAKASEARAEANLVPAKLKEERFRDLVKINAVAKQDYDNAYAALKQAEADVASA
KAAVETAKINLAYTRVTAPISGRVGRSAVTDGALVTANQPAALATIQQLGSVYVDVTQSSSDMLKLNRSLASGLLKRDAA
GQARVKLLLEDGTPYPVTGSLKFSDVTVDQSTGSITLRAVFPNPKQTLLPGMFVRAVLEEGINEAAILVPQRGVTRNAAG
LPIVMVVGADGTAQPRPIQVARTVGESWLVSGGLNPGDKVILEGLQKARPGTPVKAVPFQAQPQGAPQGAPGAAQGAPGA
AKSAPQAAAPGAAAPGAKPAAQGSPAAAQPQKK

Sequences:

>Translated_433_residues
MQSKYRAHLFTVVLLSGILGLTGCGKKEQSKGPQMGPAEVGVIEVKPERIALTTELPGRTSPYLIAEVRPQVSGIIQKRV
FTEGSDVKAGQVLYQIDPATYQAAYASAKASEARAEANLVPAKLKEERFRDLVKINAVAKQDYDNAYAALKQAEADVASA
KAAVETAKINLAYTRVTAPISGRVGRSAVTDGALVTANQPAALATIQQLGSVYVDVTQSSSDMLKLNRSLASGLLKRDAA
GQARVKLLLEDGTPYPVTGSLKFSDVTVDQSTGSITLRAVFPNPKQTLLPGMFVRAVLEEGINEAAILVPQRGVTRNAAG
LPIVMVVGADGTAQPRPIQVARTVGESWLVSGGLNPGDKVILEGLQKARPGTPVKAVPFQAQPQGAPQGAPGAAQGAPGA
AKSAPQAAAPGAAAPGAKPAAQGSPAAAQPQKK
>Mature_433_residues
MQSKYRAHLFTVVLLSGILGLTGCGKKEQSKGPQMGPAEVGVIEVKPERIALTTELPGRTSPYLIAEVRPQVSGIIQKRV
FTEGSDVKAGQVLYQIDPATYQAAYASAKASEARAEANLVPAKLKEERFRDLVKINAVAKQDYDNAYAALKQAEADVASA
KAAVETAKINLAYTRVTAPISGRVGRSAVTDGALVTANQPAALATIQQLGSVYVDVTQSSSDMLKLNRSLASGLLKRDAA
GQARVKLLLEDGTPYPVTGSLKFSDVTVDQSTGSITLRAVFPNPKQTLLPGMFVRAVLEEGINEAAILVPQRGVTRNAAG
LPIVMVVGADGTAQPRPIQVARTVGESWLVSGGLNPGDKVILEGLQKARPGTPVKAVPFQAQPQGAPQGAPGAAQGAPGA
AKSAPQAAAPGAAAPGAKPAAQGSPAAAQPQKK

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=368, Percent_Identity=59.7826086956522, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1789665, Length=377, Percent_Identity=53.5809018567639, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI1789929, Length=372, Percent_Identity=48.6559139784946, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI87082036, Length=315, Percent_Identity=26.984126984127, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI87081796, Length=175, Percent_Identity=32, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44768; Mature: 44768

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSKYRAHLFTVVLLSGILGLTGCGKKEQSKGPQMGPAEVGVIEVKPERIALTTELPGRT
CCCCHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCEEEEEECCCEEEEEECCCCCC
SPYLIAEVRPQVSGIIQKRVFTEGSDVKAGQVLYQIDPATYQAAYASAKASEARAEANLV
CCEEEEECCHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCC
PAKLKEERFRDLVKINAVAKQDYDNAYAALKQAEADVASAKAAVETAKINLAYTRVTAPI
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEECCC
SGRVGRSAVTDGALVTANQPAALATIQQLGSVYVDVTQSSSDMLKLNRSLASGLLKRDAA
CCCCCCCCCCCCEEEECCCCHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHCCC
GQARVKLLLEDGTPYPVTGSLKFSDVTVDQSTGSITLRAVFPNPKQTLLPGMFVRAVLEE
CCEEEEEEEECCCCCCEECCEEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
GINEAAILVPQRGVTRNAAGLPIVMVVGADGTAQPRPIQVARTVGESWLVSGGLNPGDKV
CCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHCCCHHEEECCCCCHHHH
ILEGLQKARPGTPVKAVPFQAQPQGAPQGAPGAAQGAPGAAKSAPQAAAPGAAAPGAKPA
HHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AQGSPAAAQPQKK
CCCCCCCCCCCCC
>Mature Secondary Structure
MQSKYRAHLFTVVLLSGILGLTGCGKKEQSKGPQMGPAEVGVIEVKPERIALTTELPGRT
CCCCHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCEEEEEECCCEEEEEECCCCCC
SPYLIAEVRPQVSGIIQKRVFTEGSDVKAGQVLYQIDPATYQAAYASAKASEARAEANLV
CCEEEEECCHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCC
PAKLKEERFRDLVKINAVAKQDYDNAYAALKQAEADVASAKAAVETAKINLAYTRVTAPI
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEECCC
SGRVGRSAVTDGALVTANQPAALATIQQLGSVYVDVTQSSSDMLKLNRSLASGLLKRDAA
CCCCCCCCCCCCEEEECCCCHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHCCC
GQARVKLLLEDGTPYPVTGSLKFSDVTVDQSTGSITLRAVFPNPKQTLLPGMFVRAVLEE
CCEEEEEEEECCCCCCEECCEEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
GINEAAILVPQRGVTRNAAGLPIVMVVGADGTAQPRPIQVARTVGESWLVSGGLNPGDKV
CCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHCCCHHEEECCCCCHHHH
ILEGLQKARPGTPVKAVPFQAQPQGAPQGAPGAAQGAPGAAKSAPQAAAPGAAAPGAKPA
HHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AQGSPAAAQPQKK
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]