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The map label for this gene is oprM [H]

Identifier: 197118748

GI number: 197118748

Start: 2744084

End: 2745508

Strand: Reverse

Name: oprM [H]

Synonym: Gbem_2367

Alternate gene names: 197118748

Gene position: 2745508-2744084 (Counterclockwise)

Preceding gene: 197118749

Following gene: 197118747

Centisome position: 59.49

GC content: 65.75

Gene sequence:

>1425_bases
ATGACACGTTTGACCGCTAGATCATTGACCCTGGGCGCGCTCCTCTTCCTTGCCGGGTGCGCCTCCATGGCTCCCAAATA
CGAAAAACCCGCTCCTCCCGTCCCCAACGCCTGGCCTACAGGCCCGTCCTATAAGGAAGGGCAGGCTGCCAAGGCTGAGC
AAAAGCCTCTGGCGGAGGTCCCGTGGCAGGAGTTCTTCCTCGATCCGCAATTGCGCAAGGTGATCGGGATTGCCCTGAAC
AACAACCGCGACCTGCGCGTAGCCGTGCTCAACATGGAGCGCTTCCGCGCCCTTTACCAGATCCAGCGGGCCGACCTTCT
GCCGCAGGTTAACGGCAACGCCGGCCTCAGCGTCCAGCGGACCCCGGACGACCTCTCCAACAACGGGAATGGTCGGACGG
TGCACCAGTACGACGTTGGGGTGGGTATCAGCTCCTACGAACTCGACCTCTTCGGGCGGGTGAGGAGCCTGAAGGACCAG
GCGCTGGAGGAATATCTCTCCACCGAACAGGCGCGCCGCAGCGTGCAGATCACCCTGGTCTCCCAGGTGGCGACCGCTTA
CCTGGCGCTGGCTTCCGACCTCGACCGGTTGAAGCTCGCCAAGGACACCCTGGCCAACCAGCAGGAGTCGTACAAGTTGA
GTAACAGCCGCTTCCAGGCCGGGGTCGCCTCCGCGCTCGACCTGCAGCAGGCGCAGACCAGCGTCGACGCGGCCCGCGTG
GACATCGCCCGCTTCACCACCTTCGTGGCGCAGGATATGAACGCGCTGAACCTGGTGGTCGGTTCCAGCGTCGGGAGCGA
TCTGCTCCCCGCCTCGCTCTCGGAAAACCTGACGCCGATCAAGGACCTCGCCCCGGGGCTTCCCTCCGACGTGCTCCTTG
CGCGGCCGGACATCCTCCAGGCCGAGTACCTTCTGCGCGGGGCCAACGCCAACATAGGCGCGGCCCGGGCCGCGTTCTTC
CCGCGCATAACTCTTACCTCCAGCGTCGGTTTCGGCAGCGACCAGTTGACCGGCCTCTTCAAGCCGGGGAACTTCGCCTG
GAGCTTCGCCCCCAGGATCACCGTCCCCATCTTCGAGGGGGGGCGCAACAAGGCCAACCTCCAGGTGGCCCAGGTGGACC
GCGACATAGCGGTGGCCCAGTATGAAAAGGCGATCCAGACCGCGTTCCGCGAGGTGGCCGACGCCCTGGCGCAGCAGGGG
ACCATCGACGAGCAGGTCTCGGCGCAGCACTCCTTGACCGGCGCCACGGCCGAGACCTTCCGCCTGTCCCAGGCCCGCTA
CCAGACCGGGATCGACAGCTACCTGAACGTGCTCGACGCCCAGCGCTCGCTTTACGCGGCGCAGCAGAACCTGATCACGG
CGCGCCTGGTGCGGCTAAGCAACCAGGTTACCCTCTACAGGGTCCTCGGGGGGGGGAGCAAGTAG

Upstream 100 bases:

>100_bases
TCTTCATCCCGTTCTTCTTCGTCATGGTGACCAAGTTCTTCATGAAGAAGAAGCCGGCAACCGAACCTGCTGCACCCCCC
GTCTCGGAGGTACATTAGAG

Downstream 100 bases:

>100_bases
CTTTCCTCTTTGTCGAAGTCAGCCGTTACCGATGTTACTGAATATGCCGGGGAACCTCCAGTCAGAGGCTCCCCGGCATT
TTGACTTGTGTCTTGAATTG

Product: RND family efflux pump outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTRLTARSLTLGALLFLAGCASMAPKYEKPAPPVPNAWPTGPSYKEGQAAKAEQKPLAEVPWQEFFLDPQLRKVIGIALN
NNRDLRVAVLNMERFRALYQIQRADLLPQVNGNAGLSVQRTPDDLSNNGNGRTVHQYDVGVGISSYELDLFGRVRSLKDQ
ALEEYLSTEQARRSVQITLVSQVATAYLALASDLDRLKLAKDTLANQQESYKLSNSRFQAGVASALDLQQAQTSVDAARV
DIARFTTFVAQDMNALNLVVGSSVGSDLLPASLSENLTPIKDLAPGLPSDVLLARPDILQAEYLLRGANANIGAARAAFF
PRITLTSSVGFGSDQLTGLFKPGNFAWSFAPRITVPIFEGGRNKANLQVAQVDRDIAVAQYEKAIQTAFREVADALAQQG
TIDEQVSAQHSLTGATAETFRLSQARYQTGIDSYLNVLDAQRSLYAAQQNLITARLVRLSNQVTLYRVLGGGSK

Sequences:

>Translated_474_residues
MTRLTARSLTLGALLFLAGCASMAPKYEKPAPPVPNAWPTGPSYKEGQAAKAEQKPLAEVPWQEFFLDPQLRKVIGIALN
NNRDLRVAVLNMERFRALYQIQRADLLPQVNGNAGLSVQRTPDDLSNNGNGRTVHQYDVGVGISSYELDLFGRVRSLKDQ
ALEEYLSTEQARRSVQITLVSQVATAYLALASDLDRLKLAKDTLANQQESYKLSNSRFQAGVASALDLQQAQTSVDAARV
DIARFTTFVAQDMNALNLVVGSSVGSDLLPASLSENLTPIKDLAPGLPSDVLLARPDILQAEYLLRGANANIGAARAAFF
PRITLTSSVGFGSDQLTGLFKPGNFAWSFAPRITVPIFEGGRNKANLQVAQVDRDIAVAQYEKAIQTAFREVADALAQQG
TIDEQVSAQHSLTGATAETFRLSQARYQTGIDSYLNVLDAQRSLYAAQQNLITARLVRLSNQVTLYRVLGGGSK
>Mature_473_residues
TRLTARSLTLGALLFLAGCASMAPKYEKPAPPVPNAWPTGPSYKEGQAAKAEQKPLAEVPWQEFFLDPQLRKVIGIALNN
NRDLRVAVLNMERFRALYQIQRADLLPQVNGNAGLSVQRTPDDLSNNGNGRTVHQYDVGVGISSYELDLFGRVRSLKDQA
LEEYLSTEQARRSVQITLVSQVATAYLALASDLDRLKLAKDTLANQQESYKLSNSRFQAGVASALDLQQAQTSVDAARVD
IARFTTFVAQDMNALNLVVGSSVGSDLLPASLSENLTPIKDLAPGLPSDVLLARPDILQAEYLLRGANANIGAARAAFFP
RITLTSSVGFGSDQLTGLFKPGNFAWSFAPRITVPIFEGGRNKANLQVAQVDRDIAVAQYEKAIQTAFREVADALAQQGT
IDEQVSAQHSLTGATAETFRLSQARYQTGIDSYLNVLDAQRSLYAAQQNLITARLVRLSNQVTLYRVLGGGSK

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=460, Percent_Identity=41.0869565217391, Blast_Score=324, Evalue=1e-89,
Organism=Escherichia coli, GI2367348, Length=492, Percent_Identity=24.390243902439, Blast_Score=101, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51472; Mature: 51341

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLTARSLTLGALLFLAGCASMAPKYEKPAPPVPNAWPTGPSYKEGQAAKAEQKPLAEV
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHC
PWQEFFLDPQLRKVIGIALNNNRDLRVAVLNMERFRALYQIQRADLLPQVNGNAGLSVQR
CHHHHHCCHHHHHHEEEEECCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCEEEEC
TPDDLSNNGNGRTVHQYDVGVGISSYELDLFGRVRSLKDQALEEYLSTEQARRSVQITLV
CCHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHH
SQVATAYLALASDLDRLKLAKDTLANQQESYKLSNSRFQAGVASALDLQQAQTSVDAARV
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
DIARFTTFVAQDMNALNLVVGSSVGSDLLPASLSENLTPIKDLAPGLPSDVLLARPDILQ
HHHHHHHHHHHCCCHHEEEECCCCCCCCCCHHHHCCCCCHHHHCCCCCCCCEECCCCHHH
AEYLLRGANANIGAARAAFFPRITLTSSVGFGSDQLTGLFKPGNFAWSFAPRITVPIFEG
HHHHHCCCCCCCCHHHHHHCCEEEEECCCCCCCHHCEEEECCCCEEEEECCEEEEEEECC
GRNKANLQVAQVDRDIAVAQYEKAIQTAFREVADALAQQGTIDEQVSAQHSLTGATAETF
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHH
RLSQARYQTGIDSYLNVLDAQRSLYAAQQNLITARLVRLSNQVTLYRVLGGGSK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCC
>Mature Secondary Structure 
TRLTARSLTLGALLFLAGCASMAPKYEKPAPPVPNAWPTGPSYKEGQAAKAEQKPLAEV
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHC
PWQEFFLDPQLRKVIGIALNNNRDLRVAVLNMERFRALYQIQRADLLPQVNGNAGLSVQR
CHHHHHCCHHHHHHEEEEECCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCEEEEC
TPDDLSNNGNGRTVHQYDVGVGISSYELDLFGRVRSLKDQALEEYLSTEQARRSVQITLV
CCHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHH
SQVATAYLALASDLDRLKLAKDTLANQQESYKLSNSRFQAGVASALDLQQAQTSVDAARV
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
DIARFTTFVAQDMNALNLVVGSSVGSDLLPASLSENLTPIKDLAPGLPSDVLLARPDILQ
HHHHHHHHHHHCCCHHEEEECCCCCCCCCCHHHHCCCCCHHHHCCCCCCCCEECCCCHHH
AEYLLRGANANIGAARAAFFPRITLTSSVGFGSDQLTGLFKPGNFAWSFAPRITVPIFEG
HHHHHCCCCCCCCHHHHHHCCEEEEECCCCCCCHHCEEEECCCCEEEEECCEEEEEEECC
GRNKANLQVAQVDRDIAVAQYEKAIQTAFREVADALAQQGTIDEQVSAQHSLTGATAETF
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHH
RLSQARYQTGIDSYLNVLDAQRSLYAAQQNLITARLVRLSNQVTLYRVLGGGSK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]