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The map label for this gene is arsB-1 [H]

Identifier: 197118730

GI number: 197118730

Start: 2726744

End: 2727799

Strand: Reverse

Name: arsB-1 [H]

Synonym: Gbem_2349

Alternate gene names: 197118730

Gene position: 2727799-2726744 (Counterclockwise)

Preceding gene: 308535298

Following gene: 197118729

Centisome position: 59.11

GC content: 56.63

Gene sequence:

>1056_bases
ATGTCTACCGGAGTTTCCCCAAAGCTGTCGTTTCTCGACCGCTATCTTACCCTTTGGATCTTCCTCGCCATGTTTGCAGG
GGTGGGAGCGGGGTACTTGTTTCCGGGAGTTGAAAGTTTCATCAACAGCTTCCAGGTAGGGACCACCAACATCCCTATTG
CCGCCGGCCTCATTCTCATGATGTACCCTCCCTTCGCCAAGGTGAAGTACGAGGAGATGCCGGAGGTCTTCCACAACAAA
CGGGTTCTCGGGCTTTCGCTGGTTCAAAACTGGCTGATAGGGCCGGTTCTGATGTTCATCCTGGCTGTCGCCTTTCTTCC
CGACAAGCCTGAGTACATGGTGGGACTCATCATGATAGGGCTCGCCAGATGCATCGCCATGGTCATCGTCTGGAACGATC
TGGCAAAGGGAAACACCGAGTACGCTGCGGGGTTGGTCGCCTTCAACAGCATCTTCCAGGTCCTCTTCTACAGCGTCTAT
GCCTGGTTCTTCATCACGGTCCTCCCGCCTCTGATCGGCCTGTCGGGCAGCATCGTGGAAGTCGGCATAGGGCAGATCGC
CAAGAGTGTCTTTATCTACCTTGGCGTCCCGTTTATCGCCGGGGCCATCACGCGCCTGGTCGGCGTCAAGGTAATGGGGA
AGGAACGCTACCACAGGGAGTTCGTGCCCAGGATCGGCCCGATCACACTGATTGCGCTTCTGTTCACCATCGTCGTCATG
TTCAGCCTGAAGGGGAACCTCATCGTTCAGCTTCCTCTCGACGTCGTCCGGATCGCGGTACCGCTGCTCATCTATTTCGT
CGCCATGTTCTTCGTCTCCTTCTGGATGGGGAAAAAGCTTGGGGCTGACTACAGCAAGACGACGACTCTTGCCTTCACCG
CCGCGAGCAACAATTTCGAATTGGCCATCGCCGTTGCGGTCGCCGTTTTCGGACTCAATTCCGGCGCCGCGTTTGCCGCG
GTGATCGGCCCATTGGTGGAAGTGCCGGTCATGATAGCCCTCGTGAATGTGGCTTTCTGGTTCCAGCGCCGCTACTTTTC
CGGTTCCAGCCTTTGA

Upstream 100 bases:

>100_bases
GAAGATCTCCTCTTTTGCCAAGGGCGCCAACTGCGCCTGATTTTTTTTCTGCCAGACATCCGCTCATTCGGATGTGAAGA
ACTGTCTGTAAGGAGGACGC

Downstream 100 bases:

>100_bases
CAGGAGACAGACGATGCAACAAAAAGTAAGGGTCTTGTTCGTTTGCATACATAACAGTGCCAGAAGCCAGATGGGCGAGG
CGTTTCTGAAGCAAGCCGGC

Product: arsenite efflux pump

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MSTGVSPKLSFLDRYLTLWIFLAMFAGVGAGYLFPGVESFINSFQVGTTNIPIAAGLILMMYPPFAKVKYEEMPEVFHNK
RVLGLSLVQNWLIGPVLMFILAVAFLPDKPEYMVGLIMIGLARCIAMVIVWNDLAKGNTEYAAGLVAFNSIFQVLFYSVY
AWFFITVLPPLIGLSGSIVEVGIGQIAKSVFIYLGVPFIAGAITRLVGVKVMGKERYHREFVPRIGPITLIALLFTIVVM
FSLKGNLIVQLPLDVVRIAVPLLIYFVAMFFVSFWMGKKLGADYSKTTTLAFTAASNNFELAIAVAVAVFGLNSGAAFAA
VIGPLVEVPVMIALVNVAFWFQRRYFSGSSL

Sequences:

>Translated_351_residues
MSTGVSPKLSFLDRYLTLWIFLAMFAGVGAGYLFPGVESFINSFQVGTTNIPIAAGLILMMYPPFAKVKYEEMPEVFHNK
RVLGLSLVQNWLIGPVLMFILAVAFLPDKPEYMVGLIMIGLARCIAMVIVWNDLAKGNTEYAAGLVAFNSIFQVLFYSVY
AWFFITVLPPLIGLSGSIVEVGIGQIAKSVFIYLGVPFIAGAITRLVGVKVMGKERYHREFVPRIGPITLIALLFTIVVM
FSLKGNLIVQLPLDVVRIAVPLLIYFVAMFFVSFWMGKKLGADYSKTTTLAFTAASNNFELAIAVAVAVFGLNSGAAFAA
VIGPLVEVPVMIALVNVAFWFQRRYFSGSSL
>Mature_350_residues
STGVSPKLSFLDRYLTLWIFLAMFAGVGAGYLFPGVESFINSFQVGTTNIPIAAGLILMMYPPFAKVKYEEMPEVFHNKR
VLGLSLVQNWLIGPVLMFILAVAFLPDKPEYMVGLIMIGLARCIAMVIVWNDLAKGNTEYAAGLVAFNSIFQVLFYSVYA
WFFITVLPPLIGLSGSIVEVGIGQIAKSVFIYLGVPFIAGAITRLVGVKVMGKERYHREFVPRIGPITLIALLFTIVVMF
SLKGNLIVQLPLDVVRIAVPLLIYFVAMFFVSFWMGKKLGADYSKTTTLAFTAASNNFELAIAVAVAVFGLNSGAAFAAV
IGPLVEVPVMIALVNVAFWFQRRYFSGSSL

Specific function: Seems to confer resistance to arsenite by allowing cells to extrude this compound. Could be part of an arsenite extrusion pump [H]

COG id: COG0798

COG function: function code P; Arsenite efflux pump ACR3 and related permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ACR3 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6325459, Length=321, Percent_Identity=35.5140186915888, Blast_Score=174, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004706
- InterPro:   IPR002657 [H]

Pfam domain/function: PF01758 SBF [H]

EC number: NA

Molecular weight: Translated: 38453; Mature: 38321

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
STGVSPKLSFLDRYLTLWIFLAMFAGVGAGYLFPGVESFINSFQVGTTNIPIAAGLILM
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHH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HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7704261; 8969508; 9384377; 7489895; 9537360 [H]