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The map label for this gene is usf [H]

Identifier: 197118684

GI number: 197118684

Start: 2676964

End: 2677758

Strand: Reverse

Name: usf [H]

Synonym: Gbem_2303

Alternate gene names: 197118684

Gene position: 2677758-2676964 (Counterclockwise)

Preceding gene: 197118686

Following gene: 197118683

Centisome position: 58.02

GC content: 60.38

Gene sequence:

>795_bases
ATGAAACAGTACCTTTTTGCACTGATGATAGGAATGGTGGCCCAGAACGCGTTCGCCGGCGTGGTCGGCAAGACGGTCGA
TTACCGCCAGGGCGATACCGTCCTGGAAGGGTATGTGGCGTATGACGATTCGGTGAAGGGGAAACGGCCGGGGGTTCTGG
TCATCCACGAGTGGACCGGGTTGGGGGCATACGAGAAGTGGCGCGCCGACCAGCTGGCGAAGCTGGGGTATGTCGCCTTC
GCGGCCGATATCTATGGCAAGGGAGTCCGCCCGGCGACCCAGGAGCTAGCGGCAAAGGAAGCCGCCAAGTATCGCGGCAA
CGACCGTAGTCTCATCCGGGCGCGTAGCGCCGCGGGTCTGGAGGCGCTATCCAAATTGCCGCAAGTGGATCCCAAGCGAC
TGGCGGTGATCGGGTATTGCTTCGGCGGGACCGCGGCGTTGGAACTGGCCCGCAGCGGTGCTGCAGTCTTGGGTACGGTC
AGTTTCCATGGCGGCCTGAGCACCCCGGTTCCCGCGGATGCCAAGAACATCAAGGGGAAGGTATTGGCGCTGCACGGGGC
GGACGACCCTTATGTCAAACCGCCCGAAGTGGAGGCATTTCAGCAGGAGATGCGCCAGGCGAAAGTAGATTGGCAGATGA
ACTATTACGGTAGTGCCGTTCACAGCTTCACCAACCCCAAGTCCGGCAACGACCCGAGCAAGGGAGTCGCTTACAACGAG
AAGGCCGACCACAGGTCGTGGGAAGCGATGAAGATCTTTTTTGACGAGATATTCGGGCAGAAGCAGAAGCATTGA

Upstream 100 bases:

>100_bases
TGTTTTTATTTCTGATAAACTAATGATAGAAATTCCGCAGCTAATGAGAGGACAACGCCGGTCACAAAATGACAGGCATT
TCAGCAGCAGGAGGTTGATC

Downstream 100 bases:

>100_bases
CTACCTCTGCTATACGGGCGGAGTCAAGGGAAACGAGCCCGAGAGTCCCCCCTCCCCCTCCGGGGGGAGGAGACAGACAG
GCGGTGACCTTCCTTCTTCG

Product: dienelactone hydrolase family protein

Products: 4-oxohex-2-enedioate

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKQYLFALMIGMVAQNAFAGVVGKTVDYRQGDTVLEGYVAYDDSVKGKRPGVLVIHEWTGLGAYEKWRADQLAKLGYVAF
AADIYGKGVRPATQELAAKEAAKYRGNDRSLIRARSAAGLEALSKLPQVDPKRLAVIGYCFGGTAALELARSGAAVLGTV
SFHGGLSTPVPADAKNIKGKVLALHGADDPYVKPPEVEAFQQEMRQAKVDWQMNYYGSAVHSFTNPKSGNDPSKGVAYNE
KADHRSWEAMKIFFDEIFGQKQKH

Sequences:

>Translated_264_residues
MKQYLFALMIGMVAQNAFAGVVGKTVDYRQGDTVLEGYVAYDDSVKGKRPGVLVIHEWTGLGAYEKWRADQLAKLGYVAF
AADIYGKGVRPATQELAAKEAAKYRGNDRSLIRARSAAGLEALSKLPQVDPKRLAVIGYCFGGTAALELARSGAAVLGTV
SFHGGLSTPVPADAKNIKGKVLALHGADDPYVKPPEVEAFQQEMRQAKVDWQMNYYGSAVHSFTNPKSGNDPSKGVAYNE
KADHRSWEAMKIFFDEIFGQKQKH
>Mature_264_residues
MKQYLFALMIGMVAQNAFAGVVGKTVDYRQGDTVLEGYVAYDDSVKGKRPGVLVIHEWTGLGAYEKWRADQLAKLGYVAF
AADIYGKGVRPATQELAAKEAAKYRGNDRSLIRARSAAGLEALSKLPQVDPKRLAVIGYCFGGTAALELARSGAAVLGTV
SFHGGLSTPVPADAKNIKGKVLALHGADDPYVKPPEVEAFQQEMRQAKVDWQMNYYGSAVHSFTNPKSGNDPSKGVAYNE
KADHRSWEAMKIFFDEIFGQKQKH

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Caenorhabditis elegans, GI17536419, Length=257, Percent_Identity=37.3540856031128, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI17532655, Length=243, Percent_Identity=37.8600823045267, Blast_Score=157, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17532653, Length=244, Percent_Identity=35.655737704918, Blast_Score=131, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17536415, Length=263, Percent_Identity=32.6996197718631, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI193204305, Length=214, Percent_Identity=35.981308411215, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17534829, Length=150, Percent_Identity=35.3333333333333, Blast_Score=94, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: 3.1.1.45

Molecular weight: Translated: 28762; Mature: 28762

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLFALMIGMVAQNAFAGVVGKTVDYRQGDTVLEGYVAYDDSVKGKRPGVLVIHEWTG
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEEECCCC
LGAYEKWRADQLAKLGYVAFAADIYGKGVRPATQELAAKEAAKYRGNDRSLIRARSAAGL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EALSKLPQVDPKRLAVIGYCFGGTAALELARSGAAVLGTVSFHGGLSTPVPADAKNIKGK
HHHHHCCCCCCCCEEEEEEECCCHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCE
VLALHGADDPYVKPPEVEAFQQEMRQAKVDWQMNYYGSAVHSFTNPKSGNDPSKGVAYNE
EEEEECCCCCCCCCCCHHHHHHHHHHHHCCEEECHHHHHHHHCCCCCCCCCCCCCCCCCC
KADHRSWEAMKIFFDEIFGQKQKH
CCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKQYLFALMIGMVAQNAFAGVVGKTVDYRQGDTVLEGYVAYDDSVKGKRPGVLVIHEWTG
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEEECCCC
LGAYEKWRADQLAKLGYVAFAADIYGKGVRPATQELAAKEAAKYRGNDRSLIRARSAAGL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EALSKLPQVDPKRLAVIGYCFGGTAALELARSGAAVLGTVSFHGGLSTPVPADAKNIKGK
HHHHHCCCCCCCCEEEEEEECCCHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCE
VLALHGADDPYVKPPEVEAFQQEMRQAKVDWQMNYYGSAVHSFTNPKSGNDPSKGVAYNE
EEEEECCCCCCCCCCCHHHHHHHHHHHHCCEEECHHHHHHHHCCCCCCCCCCCCCCCCCC
KADHRSWEAMKIFFDEIFGQKQKH
CCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-carboxymethylenebut-2-en-4-olide; H2O

Specific reaction: 4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7592443 [H]