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The map label for this gene is ccaA [H]

Identifier: 197118675

GI number: 197118675

Start: 2664329

End: 2666956

Strand: Reverse

Name: ccaA [H]

Synonym: Gbem_2294

Alternate gene names: 197118675

Gene position: 2666956-2664329 (Counterclockwise)

Preceding gene: 197118676

Following gene: 197118674

Centisome position: 57.79

GC content: 62.21

Gene sequence:

>2628_bases
ATGGATGTAATCACCACGCATCTAAACGCCGACTTCGACTGTATCGGCTCCATGGTTGCCGCGAAGAAGCTTTACCCCGA
GGCGCTCCTGGTCTTCTCAGGGGCCCAGGAGAAATCGGTTCGGGATTTCTTCCAGAAGGGGCATCCCCAGGACCTCGAAT
TCGCCCGCATCAAGGAGATTGATTTCTCCGCCATCACCCGCCTCATCGTAGTCGATTGCCAGCACTCCTCCCGCATCGGC
AAGTTCGGCGAACTCGCCAAAAGCGGCAGCGTCGAACTCCACATCTACGACCATCACCCAACCAGTTCCGGCGATATGGA
GCCCACCGGCGGCGTCATCCGCCCCTGCGGTTCCACCACGACCATCCTGGCCGGCATGTTGATCGAGCGCGACGTCCAAG
TTACCCCCTTTGAAGCCACCGTGATGATGCTGGGGATCCACGAGGACACCGGCAACCTGACGTTCCCCTCCAGCACCGCT
GAAGACTACACCGTGGCCGCGTGGCTCCTGGAGCGGGGCGCTTATCTCAACGAAGTGGCCGACTCCATCGGGCGGGAGCT
GACCGTCGAGCAGGTGTCGCTTCTAAACGACCTGCTGGTGTCGCTTAAGACCACCACCCTAAAGGGGGTCGACGTCTCGA
TAGCCCACGCCTCGGTCGACCGCTACATAGGGGACATCGCCACCCTGGCGCACATGATCCGCGACATGGAGAACCTGCAG
GCGCTTTTCCTCGTGGTCGGGATGGAGGACCGGGTCTTCGTCATCGCCAGGAGCCGGGTGCCGGAGGTGCGGGTAGGGGA
GATCCTGCATGAACTGGGCGGGGGAGGGCACGCCACGGCCGCCTCGGCCACGGTGCGCGGGCTCACCCTGATCCAGGTGC
TGGAGCGGCTGGACGCGGTGCTCAGGCGCCGGGTGGACCCAAGGCGGGTGGCGCGCGACATCATGTCGTCGCCGGTAAAG
ACCATATCCCCCGACTGCAGCATCGAGGAGGCGCGGGAGCGCCTGGTGCGCTACAACGTAAGCGCCATGCCGGTCATATC
AGAAGAGGGGGTGCTCGGGATCATCTCCAGGAAGACTGTGGAGAAGGCGCTCTACCACAACCTGAACCAGGTGCCGGTGT
CGGATTACATGCACTCCGAGTTCCACGTCGCCTCGCCCGATACCCCCATCACCGAGATCCAAAGTTACATGGTCGGGGGG
GACGCCCGCCTGGTCCCGGTGGTCTCCGAGCTGGGGTTGATTGGAGTGATCACTCGCACCGATCTGCTCCGCTACAGCTT
AGGGGGCGAGGCGCTTTACGATCTGTCGCGCGACGCACTCCAGGTGAAGAGCCGGGAGGTGGAGGCGCTGATGAACAAGC
ACCTCCCGCAGCGCACCACCGTCATCCTGCACGACCTAGGGCGCGTCGGGGACCAGCTCGACCTCACCGTCTACGCGGTC
GGAGGCTTCGTCCGCGACCTGCTTTTAGATATCCAGAACATGGACATCGACGTGACGGTGGACGGCGACGGCATCCTCTT
CGCCGAGACCTTCGCCTCCCAATTCGGCTGCCGGGTGAAAAGCCACCAGAAGTTCGGCACCGCGGTGATCGTCTTCCCGG
ACGGCTTCAAGATCGACGTCGCCAGCACCCGTCTGGAATACTACGTCTCCCCCGGCGCGCTCCCTACCGTGGAGCGCTCT
TCGCTCAAGATGGACCTGTATCGGCGCGACTTCACCGTCAACACCCTCGCCATTGCCTTGAACGGCGACTCCTTCGGCAA
GCTCATCGATTACTTCGGGGCCTACCGGGACCTGCAGTCGAGGGTGATCCGGGTGCTGCACAACCTCTCCTTTGTCGAGG
ACCCGACCCGCGTCTTCAGGGCCATCCGTTTCGAGCAGAGGCTTAACTTCAAGATCGCCAAGCACACCGAGGACCTGATC
AAGAACGCGGTGAAGATGGAATTCCTGGAAAAGCTCGGAGGGCGGAGGCTCCTCGCTGAGCTGGTTCAGATCCTGAGGGA
GAAGGAGCCGCTCAAGGGAGTCGGGCGCATGGCGTCGCTTGGGCTTTTGCGTTTCATTCACCCGGGCGTCGAACTGACCC
CCGCCATGCAGGTAGCGCTGGAGGAAACCCGCTACGTCGTCTCCTGGTTCGATCTCCTCTACCTGGAACGCCCTTACGAA
AGCTGGTCGGTCTATTTCCTGACCCTGTGCGAGGGGCTCACCGAAGAACAGTTCTGGGGAACCTGCACCCGCCTCGCCGT
CGCCGAGCATTACAAGGAGAGGCTGGTGGAGATGCGCCGGCAGAGCGAGGAGCTGATCGGAAACCTGGAAAGAAAGCTGG
CGCGTTCCGAGAAGGTCGACAACAGCGAGATCTACTTCACGCTCAGGGGGCTTCCAGTCGAGGTGCTCCTCTACCACATG
GCCAAAAGCCGTAGCGCCGAGGTAAAGCGGTGCATCTCACTTTACTTCACCAAGCTCGACGGCATGCATCCCCAGATCGG
CGGTGAGGATCTAAAGGAACTCGGCGTAGAACCGGGACCGCTGTACCGGGAGTTGCTGGACCTGGTTTTGAGCGCGCGGT
TGAACGGTAAGGTCGCGAGCCGCGAAGACGAGATCCAGTTGGTGCGCGAGGTGTTGGAGGGGGTGTAG

Upstream 100 bases:

>100_bases
TGGGGGCTACCTCCAGCAGGCTTATCTGCTACACCAACAGCGGCGAGGTGAACGGCGACCTCAAGGCAGTAGTGGCGTAC
GCCGCAATCACGGTCTCTTG

Downstream 100 bases:

>100_bases
CTTTTTTGCCACAGTACCGCTTTTTCACGTTATTTGGCCAAGTGGCCGTTTTGTAACCGTTTTGACCGAACAGCGCCCGC
AAGGTGTTGCGATTAGGGCA

Product: adenosine-specific tRNA nucleotidyltransferase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 875; Mature: 875

Protein sequence:

>875_residues
MDVITTHLNADFDCIGSMVAAKKLYPEALLVFSGAQEKSVRDFFQKGHPQDLEFARIKEIDFSAITRLIVVDCQHSSRIG
KFGELAKSGSVELHIYDHHPTSSGDMEPTGGVIRPCGSTTTILAGMLIERDVQVTPFEATVMMLGIHEDTGNLTFPSSTA
EDYTVAAWLLERGAYLNEVADSIGRELTVEQVSLLNDLLVSLKTTTLKGVDVSIAHASVDRYIGDIATLAHMIRDMENLQ
ALFLVVGMEDRVFVIARSRVPEVRVGEILHELGGGGHATAASATVRGLTLIQVLERLDAVLRRRVDPRRVARDIMSSPVK
TISPDCSIEEARERLVRYNVSAMPVISEEGVLGIISRKTVEKALYHNLNQVPVSDYMHSEFHVASPDTPITEIQSYMVGG
DARLVPVVSELGLIGVITRTDLLRYSLGGEALYDLSRDALQVKSREVEALMNKHLPQRTTVILHDLGRVGDQLDLTVYAV
GGFVRDLLLDIQNMDIDVTVDGDGILFAETFASQFGCRVKSHQKFGTAVIVFPDGFKIDVASTRLEYYVSPGALPTVERS
SLKMDLYRRDFTVNTLAIALNGDSFGKLIDYFGAYRDLQSRVIRVLHNLSFVEDPTRVFRAIRFEQRLNFKIAKHTEDLI
KNAVKMEFLEKLGGRRLLAELVQILREKEPLKGVGRMASLGLLRFIHPGVELTPAMQVALEETRYVVSWFDLLYLERPYE
SWSVYFLTLCEGLTEEQFWGTCTRLAVAEHYKERLVEMRRQSEELIGNLERKLARSEKVDNSEIYFTLRGLPVEVLLYHM
AKSRSAEVKRCISLYFTKLDGMHPQIGGEDLKELGVEPGPLYRELLDLVLSARLNGKVASREDEIQLVREVLEGV

Sequences:

>Translated_875_residues
MDVITTHLNADFDCIGSMVAAKKLYPEALLVFSGAQEKSVRDFFQKGHPQDLEFARIKEIDFSAITRLIVVDCQHSSRIG
KFGELAKSGSVELHIYDHHPTSSGDMEPTGGVIRPCGSTTTILAGMLIERDVQVTPFEATVMMLGIHEDTGNLTFPSSTA
EDYTVAAWLLERGAYLNEVADSIGRELTVEQVSLLNDLLVSLKTTTLKGVDVSIAHASVDRYIGDIATLAHMIRDMENLQ
ALFLVVGMEDRVFVIARSRVPEVRVGEILHELGGGGHATAASATVRGLTLIQVLERLDAVLRRRVDPRRVARDIMSSPVK
TISPDCSIEEARERLVRYNVSAMPVISEEGVLGIISRKTVEKALYHNLNQVPVSDYMHSEFHVASPDTPITEIQSYMVGG
DARLVPVVSELGLIGVITRTDLLRYSLGGEALYDLSRDALQVKSREVEALMNKHLPQRTTVILHDLGRVGDQLDLTVYAV
GGFVRDLLLDIQNMDIDVTVDGDGILFAETFASQFGCRVKSHQKFGTAVIVFPDGFKIDVASTRLEYYVSPGALPTVERS
SLKMDLYRRDFTVNTLAIALNGDSFGKLIDYFGAYRDLQSRVIRVLHNLSFVEDPTRVFRAIRFEQRLNFKIAKHTEDLI
KNAVKMEFLEKLGGRRLLAELVQILREKEPLKGVGRMASLGLLRFIHPGVELTPAMQVALEETRYVVSWFDLLYLERPYE
SWSVYFLTLCEGLTEEQFWGTCTRLAVAEHYKERLVEMRRQSEELIGNLERKLARSEKVDNSEIYFTLRGLPVEVLLYHM
AKSRSAEVKRCISLYFTKLDGMHPQIGGEDLKELGVEPGPLYRELLDLVLSARLNGKVASREDEIQLVREVLEGV
>Mature_875_residues
MDVITTHLNADFDCIGSMVAAKKLYPEALLVFSGAQEKSVRDFFQKGHPQDLEFARIKEIDFSAITRLIVVDCQHSSRIG
KFGELAKSGSVELHIYDHHPTSSGDMEPTGGVIRPCGSTTTILAGMLIERDVQVTPFEATVMMLGIHEDTGNLTFPSSTA
EDYTVAAWLLERGAYLNEVADSIGRELTVEQVSLLNDLLVSLKTTTLKGVDVSIAHASVDRYIGDIATLAHMIRDMENLQ
ALFLVVGMEDRVFVIARSRVPEVRVGEILHELGGGGHATAASATVRGLTLIQVLERLDAVLRRRVDPRRVARDIMSSPVK
TISPDCSIEEARERLVRYNVSAMPVISEEGVLGIISRKTVEKALYHNLNQVPVSDYMHSEFHVASPDTPITEIQSYMVGG
DARLVPVVSELGLIGVITRTDLLRYSLGGEALYDLSRDALQVKSREVEALMNKHLPQRTTVILHDLGRVGDQLDLTVYAV
GGFVRDLLLDIQNMDIDVTVDGDGILFAETFASQFGCRVKSHQKFGTAVIVFPDGFKIDVASTRLEYYVSPGALPTVERS
SLKMDLYRRDFTVNTLAIALNGDSFGKLIDYFGAYRDLQSRVIRVLHNLSFVEDPTRVFRAIRFEQRLNFKIAKHTEDLI
KNAVKMEFLEKLGGRRLLAELVQILREKEPLKGVGRMASLGLLRFIHPGVELTPAMQVALEETRYVVSWFDLLYLERPYE
SWSVYFLTLCEGLTEEQFWGTCTRLAVAEHYKERLVEMRRQSEELIGNLERKLARSEKVDNSEIYFTLRGLPVEVLLYHM
AKSRSAEVKRCISLYFTKLDGMHPQIGGEDLKELGVEPGPLYRELLDLVLSARLNGKVASREDEIQLVREVLEGV

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081691, Length=252, Percent_Identity=27.7777777777778, Blast_Score=76, Evalue=1e-14,
Organism=Escherichia coli, GI1789436, Length=285, Percent_Identity=27.719298245614, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 97947; Mature: 97947

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVITTHLNADFDCIGSMVAAKKLYPEALLVFSGAQEKSVRDFFQKGHPQDLEFARIKEI
CCEEEECCCCCHHHHHHHHHHHHHCHHHEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHC
DFSAITRLIVVDCQHSSRIGKFGELAKSGSVELHIYDHHPTSSGDMEPTGGVIRPCGSTT
CHHHHHHHHEEECCCCCCCCHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEECCCCHH
TILAGMLIERDVQVTPFEATVMMLGIHEDTGNLTFPSSTAEDYTVAAWLLERGAYLNEVA
HHHHHHHHHCCCEECCCEEEEEEEEEEECCCCEECCCCCCCHHHHHHHHHHCCCHHHHHH
DSIGRELTVEQVSLLNDLLVSLKTTTLKGVDVSIAHASVDRYIGDIATLAHMIRDMENLQ
HHHCCCCHHHHHHHHHHHHHHHHHHEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
ALFLVVGMEDRVFVIARSRVPEVRVGEILHELGGGGHATAASATVRGLTLIQVLERLDAV
EEEEEECCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LRRRVDPRRVARDIMSSPVKTISPDCSIEEARERLVRYNVSAMPVISEEGVLGIISRKTV
HHHCCCHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHCCCCCCCEECCCCCEEHHHHHHH
EKALYHNLNQVPVSDYMHSEFHVASPDTPITEIQSYMVGGDARLVPVVSELGLIGVITRT
HHHHHCCCCCCCHHHHHCCCEEECCCCCCHHHHHHHHCCCCCEEHHHHHHCCCEEEHHHH
DLLRYSLGGEALYDLSRDALQVKSREVEALMNKHLPQRTTVILHDLGRVGDQLDLTVYAV
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEEH
GGFVRDLLLDIQNMDIDVTVDGDGILFAETFASQFGCRVKSHQKFGTAVIVFPDGFKIDV
HHHHHHHHHHHHCCCEEEEECCCCEEEHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEE
ASTRLEYYVSPGALPTVERSSLKMDLYRRDFTVNTLAIALNGDSFGKLIDYFGAYRDLQS
ECCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHH
RVIRVLHNLSFVEDPTRVFRAIRFEQRLNFKIAKHTEDLIKNAVKMEFLEKLGGRRLLAE
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LVQILREKEPLKGVGRMASLGLLRFIHPGVELTPAMQVALEETRYVVSWFDLLYLERPYE
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
SWSVYFLTLCEGLTEEQFWGTCTRLAVAEHYKERLVEMRRQSEELIGNLERKLARSEKVD
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NSEIYFTLRGLPVEVLLYHMAKSRSAEVKRCISLYFTKLDGMHPQIGGEDLKELGVEPGP
CCEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCH
LYRELLDLVLSARLNGKVASREDEIQLVREVLEGV
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MDVITTHLNADFDCIGSMVAAKKLYPEALLVFSGAQEKSVRDFFQKGHPQDLEFARIKEI
CCEEEECCCCCHHHHHHHHHHHHHCHHHEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHC
DFSAITRLIVVDCQHSSRIGKFGELAKSGSVELHIYDHHPTSSGDMEPTGGVIRPCGSTT
CHHHHHHHHEEECCCCCCCCHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEECCCCHH
TILAGMLIERDVQVTPFEATVMMLGIHEDTGNLTFPSSTAEDYTVAAWLLERGAYLNEVA
HHHHHHHHHCCCEECCCEEEEEEEEEEECCCCEECCCCCCCHHHHHHHHHHCCCHHHHHH
DSIGRELTVEQVSLLNDLLVSLKTTTLKGVDVSIAHASVDRYIGDIATLAHMIRDMENLQ
HHHCCCCHHHHHHHHHHHHHHHHHHEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
ALFLVVGMEDRVFVIARSRVPEVRVGEILHELGGGGHATAASATVRGLTLIQVLERLDAV
EEEEEECCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LRRRVDPRRVARDIMSSPVKTISPDCSIEEARERLVRYNVSAMPVISEEGVLGIISRKTV
HHHCCCHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHCCCCCCCEECCCCCEEHHHHHHH
EKALYHNLNQVPVSDYMHSEFHVASPDTPITEIQSYMVGGDARLVPVVSELGLIGVITRT
HHHHHCCCCCCCHHHHHCCCEEECCCCCCHHHHHHHHCCCCCEEHHHHHHCCCEEEHHHH
DLLRYSLGGEALYDLSRDALQVKSREVEALMNKHLPQRTTVILHDLGRVGDQLDLTVYAV
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEEH
GGFVRDLLLDIQNMDIDVTVDGDGILFAETFASQFGCRVKSHQKFGTAVIVFPDGFKIDV
HHHHHHHHHHHHCCCEEEEECCCCEEEHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEE
ASTRLEYYVSPGALPTVERSSLKMDLYRRDFTVNTLAIALNGDSFGKLIDYFGAYRDLQS
ECCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHH
RVIRVLHNLSFVEDPTRVFRAIRFEQRLNFKIAKHTEDLIKNAVKMEFLEKLGGRRLLAE
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LVQILREKEPLKGVGRMASLGLLRFIHPGVELTPAMQVALEETRYVVSWFDLLYLERPYE
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
SWSVYFLTLCEGLTEEQFWGTCTRLAVAEHYKERLVEMRRQSEELIGNLERKLARSEKVD
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NSEIYFTLRGLPVEVLLYHMAKSRSAEVKRCISLYFTKLDGMHPQIGGEDLKELGVEPGP
CCEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCH
LYRELLDLVLSARLNGKVASREDEIQLVREVLEGV
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA