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The map label for this gene is macA [H]

Identifier: 197118584

GI number: 197118584

Start: 2562614

End: 2563873

Strand: Reverse

Name: macA [H]

Synonym: Gbem_2203

Alternate gene names: 197118584

Gene position: 2563873-2562614 (Counterclockwise)

Preceding gene: 197118586

Following gene: 197118583

Centisome position: 55.55

GC content: 63.25

Gene sequence:

>1260_bases
GTGAAAAAAATCATAGGCGCCGTTTTACTGCTGCTGGTCCTGAGTGTGGCCGGCTACTTCTACTTTAAACAGCCGCCGCA
ACCGCAATACAAGACGCAGAAGGTGGAGCGCGGATCCATCGTCTCTACCGTTTCGGCGACAGGGACGCTCAACGCCGTGG
TGACCGTGCAGGTGGGAACGCAGGTTTCCGGCACCATCGCCAAGCTTTACGTCGACTTCAACTCACAGGTCAAGAAGGGG
CAGCCGATCGCGCAGATCGACCCGGCCCTGTTTCTCTCTGCGGTGCAGCAGTCCCGCGGCAACGCCCTGAACGCCGAGGC
GAGCCTCGCCAAGGCGCGGGTGACCCTAGCCGATGCGAAGAGGACCTACAACCGCAACAAGGCGCTTCTGGTCGAGGGGA
TCATCGCCCAGAGCGACCTCGACGCCGCCCAGACCGCCTACGACGCGGCACTCACCGGGGTGCGCGGGGCCGAAGCGGTG
CTCTTGCAGACCCGGGGCGCCCTCAGGCAGGCGGAGACGAATCTCAACTATGCCACCATCAAGTCGCCGGTCGACGGCAT
CGTGGTCTCCCGCAACGTGGACGTGGGGCAGACTGTCGCTGCCTCCTTCCAGACGCCGACCCTCTTCACCATCGCGCAGG
ACTTGACCAAGATGCAGATCGACACCAGCATCGACGAGTCGGACATAAGCCGGCTGAAGGTGGGGCAGAAGGCTGAATTC
ACCGTGGACGCTTATCCCGAGCAGACCTTCGAGGGGACCGTGGTGCAGATCCGGAACGCGCCGGTGGTAACGCAGAACGT
GGTCACCTACGTGGCCGTGATCGGCGTCGACAACCGGCAGCTCCTGTTGAAGCCTGGGATGACGGCCAACGTGGCAGTGC
AGACCATGAGAAAGGACGACCTGCTCAAGGTCCCCACCGCGGCGCTCAGGTTCAAGCCCAAAGCGGAGAAGGGGAAAGAG
AAGGACAAAGGGAAGGGTGGCAAGGGCGCCGGCATGACCGCGGCAGCCCCGAAGGGGAGTGTCAGGATGCAGGGCGAGGG
GAGGAGCGCCGCCGCCAACGGTACGAAGCAGACCGTCTACCTGTTGAAGGGGGAGGGGAAAGCCGTTCCCGTAGCCATCA
TCACGGGCATCAGCGACGGGGCTTCTGTCGAGGTGGTAAGCGGGGCCGTGAAGGAAGGGGACGACGTCATCATCGAGCAG
GTGAGCCAGGATAAGAAGAAAAGCGGCATGGGCGGCTCGCCGATGGGACCGAGGTTCTAG

Upstream 100 bases:

>100_bases
CGGGTTCATTATTCTTGCACAATCGCCGTTTAACCTGTTTCCCGTCCTGCGCCGGTTCCTTCCGGCATTCCGAAGGCGAT
TTCATCGAAGGGGTGCACCA

Downstream 100 bases:

>100_bases
ATGAACGAAGTGGTTCGCCTCATCGACGTCGCCAAGGTTTACACCATGGGCGAGGAGCGCGTCGAGGCCATGAAGGGAGT
CTCTTTCGCCGTCAGCCGCG

Product: RND family efflux pump membrane fusion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MKKIIGAVLLLLVLSVAGYFYFKQPPQPQYKTQKVERGSIVSTVSATGTLNAVVTVQVGTQVSGTIAKLYVDFNSQVKKG
QPIAQIDPALFLSAVQQSRGNALNAEASLAKARVTLADAKRTYNRNKALLVEGIIAQSDLDAAQTAYDAALTGVRGAEAV
LLQTRGALRQAETNLNYATIKSPVDGIVVSRNVDVGQTVAASFQTPTLFTIAQDLTKMQIDTSIDESDISRLKVGQKAEF
TVDAYPEQTFEGTVVQIRNAPVVTQNVVTYVAVIGVDNRQLLLKPGMTANVAVQTMRKDDLLKVPTAALRFKPKAEKGKE
KDKGKGGKGAGMTAAAPKGSVRMQGEGRSAAANGTKQTVYLLKGEGKAVPVAIITGISDGASVEVVSGAVKEGDDVIIEQ
VSQDKKKSGMGGSPMGPRF

Sequences:

>Translated_419_residues
MKKIIGAVLLLLVLSVAGYFYFKQPPQPQYKTQKVERGSIVSTVSATGTLNAVVTVQVGTQVSGTIAKLYVDFNSQVKKG
QPIAQIDPALFLSAVQQSRGNALNAEASLAKARVTLADAKRTYNRNKALLVEGIIAQSDLDAAQTAYDAALTGVRGAEAV
LLQTRGALRQAETNLNYATIKSPVDGIVVSRNVDVGQTVAASFQTPTLFTIAQDLTKMQIDTSIDESDISRLKVGQKAEF
TVDAYPEQTFEGTVVQIRNAPVVTQNVVTYVAVIGVDNRQLLLKPGMTANVAVQTMRKDDLLKVPTAALRFKPKAEKGKE
KDKGKGGKGAGMTAAAPKGSVRMQGEGRSAAANGTKQTVYLLKGEGKAVPVAIITGISDGASVEVVSGAVKEGDDVIIEQ
VSQDKKKSGMGGSPMGPRF
>Mature_419_residues
MKKIIGAVLLLLVLSVAGYFYFKQPPQPQYKTQKVERGSIVSTVSATGTLNAVVTVQVGTQVSGTIAKLYVDFNSQVKKG
QPIAQIDPALFLSAVQQSRGNALNAEASLAKARVTLADAKRTYNRNKALLVEGIIAQSDLDAAQTAYDAALTGVRGAEAV
LLQTRGALRQAETNLNYATIKSPVDGIVVSRNVDVGQTVAASFQTPTLFTIAQDLTKMQIDTSIDESDISRLKVGQKAEF
TVDAYPEQTFEGTVVQIRNAPVVTQNVVTYVAVIGVDNRQLLLKPGMTANVAVQTMRKDDLLKVPTAALRFKPKAEKGKE
KDKGKGGKGAGMTAAAPKGSVRMQGEGRSAAANGTKQTVYLLKGEGKAVPVAIITGISDGASVEVVSGAVKEGDDVIIEQ
VSQDKKKSGMGGSPMGPRF

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=301, Percent_Identity=32.5581395348837, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI87082036, Length=270, Percent_Identity=28.5185185185185, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789929, Length=177, Percent_Identity=32.2033898305085, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790519, Length=244, Percent_Identity=29.0983606557377, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1789041, Length=233, Percent_Identity=26.1802575107296, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44224; Mature: 44224

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIIGAVLLLLVLSVAGYFYFKQPPQPQYKTQKVERGSIVSTVSATGTLNAVVTVQVGT
CHHHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHCCCCCEEEEEECCCCEEEEEEEEECC
QVSGTIAKLYVDFNSQVKKGQPIAQIDPALFLSAVQQSRGNALNAEASLAKARVTLADAK
CCCCEEEEEEEECCCCCCCCCCHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHEEEEHHHH
RTYNRNKALLVEGIIAQSDLDAAQTAYDAALTGVRGAEAVLLQTRGALRQAETNLNYATI
HHHCCCCEEEEEEEHHCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHCCCCEEEE
KSPVDGIVVSRNVDVGQTVAASFQTPTLFTIAQDLTKMQIDTSIDESDISRLKVGQKAEF
CCCCCCEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHEECCCCCCHHHHHHHCCCCCCEE
TVDAYPEQTFEGTVVQIRNAPVVTQNVVTYVAVIGVDNRQLLLKPGMTANVAVQTMRKDD
EEECCCCCCCCCEEEEEECCCCCHHHHEEEEEEEECCCCEEEECCCCCCCCEEEECCCCC
LLKVPTAALRFKPKAEKGKEKDKGKGGKGAGMTAAAPKGSVRMQGEGRSAAANGTKQTVY
CEECCCHHHEECCCHHCCCCHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEE
LLKGEGKAVPVAIITGISDGASVEVVSGAVKEGDDVIIEQVSQDKKKSGMGGSPMGPRF
EEECCCCEEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKKIIGAVLLLLVLSVAGYFYFKQPPQPQYKTQKVERGSIVSTVSATGTLNAVVTVQVGT
CHHHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHCCCCCEEEEEECCCCEEEEEEEEECC
QVSGTIAKLYVDFNSQVKKGQPIAQIDPALFLSAVQQSRGNALNAEASLAKARVTLADAK
CCCCEEEEEEEECCCCCCCCCCHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHEEEEHHHH
RTYNRNKALLVEGIIAQSDLDAAQTAYDAALTGVRGAEAVLLQTRGALRQAETNLNYATI
HHHCCCCEEEEEEEHHCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHCCCCEEEE
KSPVDGIVVSRNVDVGQTVAASFQTPTLFTIAQDLTKMQIDTSIDESDISRLKVGQKAEF
CCCCCCEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHEECCCCCCHHHHHHHCCCCCCEE
TVDAYPEQTFEGTVVQIRNAPVVTQNVVTYVAVIGVDNRQLLLKPGMTANVAVQTMRKDD
EEECCCCCCCCCEEEEEECCCCCHHHHEEEEEEEECCCCEEEECCCCCCCCEEEECCCCC
LLKVPTAALRFKPKAEKGKEKDKGKGGKGAGMTAAAPKGSVRMQGEGRSAAANGTKQTVY
CEECCCHHHEECCCHHCCCCHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEE
LLKGEGKAVPVAIITGISDGASVEVVSGAVKEGDDVIIEQVSQDKKKSGMGGSPMGPRF
EEECCCCEEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]