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The map label for this gene is paaK-1 [H]

Identifier: 197118565

GI number: 197118565

Start: 2543594

End: 2544895

Strand: Reverse

Name: paaK-1 [H]

Synonym: Gbem_2184

Alternate gene names: 197118565

Gene position: 2544895-2543594 (Counterclockwise)

Preceding gene: 197118566

Following gene: 197118564

Centisome position: 55.14

GC content: 60.83

Gene sequence:

>1302_bases
ATGTCAATTTGGGATCCGGATTACGAATGCATGCCGCGCGAGGAGATGGAGCAACTCCAGTTGGAGCGCCTCCAGGCTAC
CCTCAACCGCGTGTACAAAAACGTCACCTGCTACCGGAACAAGTTCAAGGAACTGGGAATTGTCCCCGAGGATGTCACAT
CCCTCGCCGACCTCTCGAAGCTTCCCTTCACCACGAAAGAGGACCTGCGCCTCAACTATCCCTACGGCATGTTCGCGGTG
CCGCTTCGGGAGGTGGTGCGCATCCACTCCTCCAGCGGCACCACCGGCAAGCCCACCGTCGTCGGCTACACCAAGCAGGA
CGTGAAGGTCTGGTCCAACCTGGTGGCGCGCTTCATGACCGCTGCCGGCGTCAACCACGACGACGTGGTGCAGATCGCCT
TCGGCTACGGCCTCTTCACCGGAGCTTTCGGCCTCCATTACGGCTCGGAGATGATCGGTGCCTCGGTCATCCCGATGGGC
GCCGGGAACACCGAAAAGCAGATCATGATCATGCAGGACTACCGCACCACCGCCCTGGTCTCGACGCCTAGCTACGCGGT
CACCATAGCCGAGCGCATGGAGAAGATGGGGATCGACCCCAAGAGCCTCTGCCTCAAAGTGGGGCTTTTCGGCGGCGAGC
CGTGGTCCGAGGCGATGCGCCGCGAGATCGAGAGCAGGCTCGGCATCTCGGCCACGGACAACTACGGGCTTTCCGAGGTG
ATCGGACCGGGCGTCGCCGGCGAATGCCAGTGCAAGTGCGGCATGCATATCTCTGAGGACGCCTTCTTGGCCGAGATCAT
CGACCCGGATACCGGCAAGACGCTCCCGCCGGGAAGCATCGGCGAACTGGTACTCACCTCGCTCACCAAGGAAGCGTTCC
CGATGGTCCGCTACCGCACCCGCGACATCACCTCGCTCGACTACACAAAGTGCGACTGCGGCAGGACCACGGTGCGCATG
AAGAAGACCATGGGGCGCTCCGACGACATGCTGATCATCAAGGGCGTGAACGTCTACCCGTCCCAGATCGAGGACGTCCT
CTTCGCCGTCGACGGGTGCCAGCCGCACTACCAACTGGTGGTCGACCGCAAGGGAGCGCTGGATACCCTGGAGATCAGGA
TAGAGGTGACCGAAAACATCTTCTTCGACGAGATGAAGCTGCAGAAGGCTTTCCTCGACAACGTGGAGCGCCGCATTGAC
TCAGTGCTAGGGGTTGGTGCCGTCGTAAAACTGGTTGAGCCGAACAGCATCCCGAGGGCCGAAGGTAAAGCCTCCAGAGT
AATTGACAACAGAAAAATCTAG

Upstream 100 bases:

>100_bases
GAATTGATTCATTCGGGTTTAATCGTCAATTGTCAATTGTCAATTATCAATTGGTTCTAGACGTTGGTTTTAGACGTCAA
CTGGTTTAAAGAGAGGGTTT

Downstream 100 bases:

>100_bases
ACTAGCATTGGTTCCACCTTTCCTGACAGATCAGCTCAGTCAGGAGAGGATGAATACTCAAAGGGCATAGCCCTCACAAG
GAGGAATCATATGAAGAAGT

Product: phenylacetate--coenzyme A ligase

Products: NA

Alternate protein names: Phenylacetyl-CoA ligase; PA-CoA ligase [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSIWDPDYECMPREEMEQLQLERLQATLNRVYKNVTCYRNKFKELGIVPEDVTSLADLSKLPFTTKEDLRLNYPYGMFAV
PLREVVRIHSSSGTTGKPTVVGYTKQDVKVWSNLVARFMTAAGVNHDDVVQIAFGYGLFTGAFGLHYGSEMIGASVIPMG
AGNTEKQIMIMQDYRTTALVSTPSYAVTIAERMEKMGIDPKSLCLKVGLFGGEPWSEAMRREIESRLGISATDNYGLSEV
IGPGVAGECQCKCGMHISEDAFLAEIIDPDTGKTLPPGSIGELVLTSLTKEAFPMVRYRTRDITSLDYTKCDCGRTTVRM
KKTMGRSDDMLIIKGVNVYPSQIEDVLFAVDGCQPHYQLVVDRKGALDTLEIRIEVTENIFFDEMKLQKAFLDNVERRID
SVLGVGAVVKLVEPNSIPRAEGKASRVIDNRKI

Sequences:

>Translated_433_residues
MSIWDPDYECMPREEMEQLQLERLQATLNRVYKNVTCYRNKFKELGIVPEDVTSLADLSKLPFTTKEDLRLNYPYGMFAV
PLREVVRIHSSSGTTGKPTVVGYTKQDVKVWSNLVARFMTAAGVNHDDVVQIAFGYGLFTGAFGLHYGSEMIGASVIPMG
AGNTEKQIMIMQDYRTTALVSTPSYAVTIAERMEKMGIDPKSLCLKVGLFGGEPWSEAMRREIESRLGISATDNYGLSEV
IGPGVAGECQCKCGMHISEDAFLAEIIDPDTGKTLPPGSIGELVLTSLTKEAFPMVRYRTRDITSLDYTKCDCGRTTVRM
KKTMGRSDDMLIIKGVNVYPSQIEDVLFAVDGCQPHYQLVVDRKGALDTLEIRIEVTENIFFDEMKLQKAFLDNVERRID
SVLGVGAVVKLVEPNSIPRAEGKASRVIDNRKI
>Mature_432_residues
SIWDPDYECMPREEMEQLQLERLQATLNRVYKNVTCYRNKFKELGIVPEDVTSLADLSKLPFTTKEDLRLNYPYGMFAVP
LREVVRIHSSSGTTGKPTVVGYTKQDVKVWSNLVARFMTAAGVNHDDVVQIAFGYGLFTGAFGLHYGSEMIGASVIPMGA
GNTEKQIMIMQDYRTTALVSTPSYAVTIAERMEKMGIDPKSLCLKVGLFGGEPWSEAMRREIESRLGISATDNYGLSEVI
GPGVAGECQCKCGMHISEDAFLAEIIDPDTGKTLPPGSIGELVLTSLTKEAFPMVRYRTRDITSLDYTKCDCGRTTVRMK
KTMGRSDDMLIIKGVNVYPSQIEDVLFAVDGCQPHYQLVVDRKGALDTLEIRIEVTENIFFDEMKLQKAFLDNVERRIDS
VLGVGAVVKLVEPNSIPRAEGKASRVIDNRKI

Specific function: Catalyzes the activation of phenylacetic acid to phenylacetyl-CoA [H]

COG id: COG1541

COG function: function code H; Coenzyme F390 synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787664, Length=422, Percent_Identity=50.2369668246445, Blast_Score=428, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011880 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.30 [H]

Molecular weight: Translated: 48178; Mature: 48047

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIWDPDYECMPREEMEQLQLERLQATLNRVYKNVTCYRNKFKELGIVPEDVTSLADLSK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LPFTTKEDLRLNYPYGMFAVPLREVVRIHSSSGTTGKPTVVGYTKQDVKVWSNLVARFMT
CCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHH
AAGVNHDDVVQIAFGYGLFTGAFGLHYGSEMIGASVIPMGAGNTEKQIMIMQDYRTTALV
HCCCCCCCEEEEEHHHHHHHHHHHHHCCHHHHCCEEEECCCCCCCCEEEEEECCCCEEEE
STPSYAVTIAERMEKMGIDPKSLCLKVGLFGGEPWSEAMRREIESRLGISATDNYGLSEV
ECCCHHHHHHHHHHHHCCCHHHHHEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHH
IGPGVAGECQCKCGMHISEDAFLAEIIDPDTGKTLPPGSIGELVLTSLTKEAFPMVRYRT
HCCCCCCCCEEECCCEECCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEC
RDITSLDYTKCDCGRTTVRMKKTMGRSDDMLIIKGVNVYPSQIEDVLFAVDGCQPHYQLV
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEEEE
VDRKGALDTLEIRIEVTENIFFDEMKLQKAFLDNVERRIDSVLGVGAVVKLVEPNSIPRA
EECCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCC
EGKASRVIDNRKI
CCCHHHHHCCCCC
>Mature Secondary Structure 
SIWDPDYECMPREEMEQLQLERLQATLNRVYKNVTCYRNKFKELGIVPEDVTSLADLSK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LPFTTKEDLRLNYPYGMFAVPLREVVRIHSSSGTTGKPTVVGYTKQDVKVWSNLVARFMT
CCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHH
AAGVNHDDVVQIAFGYGLFTGAFGLHYGSEMIGASVIPMGAGNTEKQIMIMQDYRTTALV
HCCCCCCCEEEEEHHHHHHHHHHHHHCCHHHHCCEEEECCCCCCCCEEEEEECCCCEEEE
STPSYAVTIAERMEKMGIDPKSLCLKVGLFGGEPWSEAMRREIESRLGISATDNYGLSEV
ECCCHHHHHHHHHHHHCCCHHHHHEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHH
IGPGVAGECQCKCGMHISEDAFLAEIIDPDTGKTLPPGSIGELVLTSLTKEAFPMVRYRT
HCCCCCCCCEEECCCEECCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEC
RDITSLDYTKCDCGRTTVRMKKTMGRSDDMLIIKGVNVYPSQIEDVLFAVDGCQPHYQLV
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEEEE
VDRKGALDTLEIRIEVTENIFFDEMKLQKAFLDNVERRIDSVLGVGAVVKLVEPNSIPRA
EECCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCC
EGKASRVIDNRKI
CCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]