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The map label for this gene is fdnH-3 [H]

Identifier: 197118561

GI number: 197118561

Start: 2538738

End: 2539532

Strand: Reverse

Name: fdnH-3 [H]

Synonym: Gbem_2180

Alternate gene names: 197118561

Gene position: 2539532-2538738 (Counterclockwise)

Preceding gene: 308535291

Following gene: 197118560

Centisome position: 55.03

GC content: 64.53

Gene sequence:

>795_bases
ATGACAGCTACCGGTTTCTTCACCGATACGAGTGTCTGCATCGGCTGCAAGGCCTGCGAGGTCGCCTGCAAGCAGTGGAA
CCAGCTCCCAGCGGACGGCAACTTCTTCAGCGGGCGATCCTACGACAACACCATCCACCTTGAAGCCACCACCTGGCGGC
ATGTGGCCTTCGTGGAGCGCTCCCTGCAGTTAAGCGGCCAGATAACGGCGCGCCCCTTCTCCTGGCTGCTCATGTCCGAC
GTCTGCAAGCACTGCGAACGCGCCGGATGCCTGGAGGCCTGCCCGACTGGCGCCATCGTCCGCACCGAGTTCGAGAGCGT
GTACGTGCAACCCGACGTCTGCAACGGCTGCGGCTACTGCGTGGTCTGCTGCCCCTTCGGGGTGATCAACCGCCGCGAGG
ACGACGGCCGCGCCTGGAAATGCACCTTGTGCTACGACCGCCAGAAAGCCGGAGGGGAGCCCGCCTGCGCCAAGGCCTGT
CCCACCGATTCCATCACCTTCGGAAAACTCGACGAACTGCGAGAGATAGCCCGCAAACGCCTGGAGCTGCTGCATAGCCG
CGGGGTCCACGAGGCGTACCTTTACGGGATGACGGACGACGACCAGCCCGGCACCGAGGGGCTGCACGCCTTTTTCCTGC
TGCTGGATCGTCCCGAGGCGTACAACCTCCCTCCCAAACCGACCGTGCCGACCAAGGGAGTAGGGGCTTCGTGGGGGACG
ATGGGTGTGGCGGCGGCAGGCATGGTCGCTGCCGCGGTGGCTGCGGTTCGATTCGTGGAAGGAGGGAAAAAATGA

Upstream 100 bases:

>100_bases
TCGGACGGCCCCAAACGCGACCAACCCCCGGTTCAGGATAAACCCGAAGGAAGGCACGGCCTTAAGGCAGGAAAAACCGA
ATTGACGGAGGAGGCCTGAC

Downstream 100 bases:

>100_bases
GCCAAGCTCTGACCAGTGGCTTGAAGATCGGAAGGGAGATGGACCCGGATTTGGCGATGCTCACGGGGGAGGGGGCGCAG
CAGCGGGTAAAGCAGGCGGA

Product: iron-sulfur cluster-binding hydrogenase protein

Products: CO2; NADH

Alternate protein names: Formate dehydrogenase subunit beta; FDH subunit beta [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MTATGFFTDTSVCIGCKACEVACKQWNQLPADGNFFSGRSYDNTIHLEATTWRHVAFVERSLQLSGQITARPFSWLLMSD
VCKHCERAGCLEACPTGAIVRTEFESVYVQPDVCNGCGYCVVCCPFGVINRREDDGRAWKCTLCYDRQKAGGEPACAKAC
PTDSITFGKLDELREIARKRLELLHSRGVHEAYLYGMTDDDQPGTEGLHAFFLLLDRPEAYNLPPKPTVPTKGVGASWGT
MGVAAAGMVAAAVAAVRFVEGGKK

Sequences:

>Translated_264_residues
MTATGFFTDTSVCIGCKACEVACKQWNQLPADGNFFSGRSYDNTIHLEATTWRHVAFVERSLQLSGQITARPFSWLLMSD
VCKHCERAGCLEACPTGAIVRTEFESVYVQPDVCNGCGYCVVCCPFGVINRREDDGRAWKCTLCYDRQKAGGEPACAKAC
PTDSITFGKLDELREIARKRLELLHSRGVHEAYLYGMTDDDQPGTEGLHAFFLLLDRPEAYNLPPKPTVPTKGVGASWGT
MGVAAAGMVAAAVAAVRFVEGGKK
>Mature_263_residues
TATGFFTDTSVCIGCKACEVACKQWNQLPADGNFFSGRSYDNTIHLEATTWRHVAFVERSLQLSGQITARPFSWLLMSDV
CKHCERAGCLEACPTGAIVRTEFESVYVQPDVCNGCGYCVVCCPFGVINRREDDGRAWKCTLCYDRQKAGGEPACAKACP
TDSITFGKLDELREIARKRLELLHSRGVHEAYLYGMTDDDQPGTEGLHAFFLLLDRPEAYNLPPKPTVPTKGVGASWGTM
GVAAAGMVAAAVAAVRFVEGGKK

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787749, Length=248, Percent_Identity=39.5161290322581, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1790326, Length=251, Percent_Identity=38.2470119521912, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI1787122, Length=174, Percent_Identity=36.7816091954023, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787872, Length=174, Percent_Identity=34.4827586206897, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI2367345, Length=182, Percent_Identity=33.5164835164835, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789370, Length=194, Percent_Identity=29.3814432989691, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1789067, Length=197, Percent_Identity=31.4720812182741, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI226510944, Length=164, Percent_Identity=31.7073170731707, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788811, Length=205, Percent_Identity=26.3414634146341, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87082179, Length=180, Percent_Identity=28.8888888888889, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082180, Length=171, Percent_Identity=32.1637426900585, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI2367245, Length=179, Percent_Identity=29.608938547486, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1787478, Length=86, Percent_Identity=32.5581395348837, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 28767; Mature: 28636

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
6.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATGFFTDTSVCIGCKACEVACKQWNQLPADGNFFSGRSYDNTIHLEATTWRHVAFVER
CCCCCEECCCCCEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHH
SLQLSGQITARPFSWLLMSDVCKHCERAGCLEACPTGAIVRTEFESVYVQPDVCNGCGYC
HHHHCCEEECCHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEECCEEEECCCCCCCCCEE
VVCCPFGVINRREDDGRAWKCTLCYDRQKAGGEPACAKACPTDSITFGKLDELREIARKR
EEECCCCHHCCCCCCCCEEEEEEEECHHHCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHH
LELLHSRGVHEAYLYGMTDDDQPGTEGLHAFFLLLDRPEAYNLPPKPTVPTKGVGASWGT
HHHHHHCCCCEEEEECCCCCCCCCHHHHEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCH
MGVAAAGMVAAAVAAVRFVEGGKK
HHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TATGFFTDTSVCIGCKACEVACKQWNQLPADGNFFSGRSYDNTIHLEATTWRHVAFVER
CCCCEECCCCCEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHH
SLQLSGQITARPFSWLLMSDVCKHCERAGCLEACPTGAIVRTEFESVYVQPDVCNGCGYC
HHHHCCEEECCHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEECCEEEECCCCCCCCCEE
VVCCPFGVINRREDDGRAWKCTLCYDRQKAGGEPACAKACPTDSITFGKLDELREIARKR
EEECCCCHHCCCCCCCCEEEEEEEECHHHCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHH
LELLHSRGVHEAYLYGMTDDDQPGTEGLHAFFLLLDRPEAYNLPPKPTVPTKGVGASWGT
HHHHHHCCCCEEEEECCCCCCCCCHHHHEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCH
MGVAAAGMVAAAVAAVRFVEGGKK
HHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]