Click here to switch to the map view.

The map label for this gene is zraS [H]

Identifier: 197118542

GI number: 197118542

Start: 2515491

End: 2516729

Strand: Reverse

Name: zraS [H]

Synonym: Gbem_2161

Alternate gene names: 197118542

Gene position: 2516729-2515491 (Counterclockwise)

Preceding gene: 197118543

Following gene: 197118541

Centisome position: 54.53

GC content: 61.1

Gene sequence:

>1239_bases
ATGTTCAGAAAAGCGACGCTGATAATCGGAATATTCGCAACCTGTTCCCTGCTTTGGTTCACCCTCTACAACTACCGCCA
GGCCGGCCCCATCGCCCAGGAGAACCTGCGCGGACTCGCCCTCACGCTTACCTCCGCCATCGAGAACCTCGCGGTTAACG
ACCCGAACCTTGCCAGCCTTGCCAAATTCCGCACTAACGATATCGCCTACTTCGCCCTGGTGGACAGCCAGGGCCGTTTC
CGCTTCCATTCGAACCCGGACCTGATCGGTGCCTCCCTGCCGGAAGCCGAACGCAAAGCTGCACCAAAACTTGAGCGATC
CGAGTCGCGAGTCGCCCTGGGGACGGGTGAGCAGGCATTCCTGGTAACAACTCCCATCAACTTAAACGGCGAAATTCTCT
CCCTTCATCTCACCCTGCACACCTATCGCGCCGATGCTGTCGTGCGCAGCGCCAAGCTGAACCTGACCGTAATGCTTGCG
CTGATTGCGACAGGTTGGCTCCTTAGTCTCGGCCTTTTCCGCTATGCCCGCAGGGAAGAACTGCACCAGGCCGAAATGAC
GCGCAAGGTGAACCTAGCCAAGCTGGGCGAAATGGGGGCGCTCCTGGCCCACGAAATCAGGAACCCTCTTGCCGGCATCA
AGGGGTTCGCCCAGGTGATCGCCAAAAAGCCGCAGGAGGCACGCAACGCCGCCTTCGCACAAAACATCGTCACCGAGGTG
GTGCGGCTGGAGGCGCTGGTTAACGACCTTTTGGCCTATGCCGCCGGCGACGGAGCACAGCGGGCGCCCTTCGACCTGGG
CGAACTGATCGACAACACCCTCTCGCTCCTGGCCCCGGAGGCAGTCGAGCGCGAGGTATCCGTTTGCTGCAGCAGCACCT
GCTCCTTCTTCGTGATGGGAAACAGGGACCGGATCGAGCAGACGCTCCTCAACCTGGGTAAAAACGCGCTGCAGGCGATG
GACGCAGGGGGCGTACTGGAAATAGCGTGCGCCTCGGCCGGGGGACAAGCGCAAATCAGCATCAAGGATACCGGGCAGGG
GATAGCGGAGGCGGACCTTCCCAAGGTCTTCGAGCCATTCTTCACCACAAGGGCCAGGGGAACCGGGCTGGGACTCGCCC
TTTGCAAAAAGGTGATCGAAGAGCACGGCGGAACCATCGGGTTGGAGAGCAGGATAGGGGAGGGGACCACGGTGACCCTC
GCCTTGCCTTTGCTCCCCGCGGACAAGGAGCAGACATGA

Upstream 100 bases:

>100_bases
GGGCAAACGGAGATGATGCTCGAAGTGCGGGAGCATTGATGGAAACAGTGAAAGACGCGGTTTTTGAGCGGCAGCCTGAA
AAATGGGCTACGGGAAGCGA

Downstream 100 bases:

>100_bases
AAGGAAACATTCTGGTGGTCGAGGATGACGGCACCTTCCGCGCTTTTCTGGAGACAGTGCTGCAGGACGACGGGCACCAG
GTAAAGACCGCCGCCGATGG

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MFRKATLIIGIFATCSLLWFTLYNYRQAGPIAQENLRGLALTLTSAIENLAVNDPNLASLAKFRTNDIAYFALVDSQGRF
RFHSNPDLIGASLPEAERKAAPKLERSESRVALGTGEQAFLVTTPINLNGEILSLHLTLHTYRADAVVRSAKLNLTVMLA
LIATGWLLSLGLFRYARREELHQAEMTRKVNLAKLGEMGALLAHEIRNPLAGIKGFAQVIAKKPQEARNAAFAQNIVTEV
VRLEALVNDLLAYAAGDGAQRAPFDLGELIDNTLSLLAPEAVEREVSVCCSSTCSFFVMGNRDRIEQTLLNLGKNALQAM
DAGGVLEIACASAGGQAQISIKDTGQGIAEADLPKVFEPFFTTRARGTGLGLALCKKVIEEHGGTIGLESRIGEGTTVTL
ALPLLPADKEQT

Sequences:

>Translated_412_residues
MFRKATLIIGIFATCSLLWFTLYNYRQAGPIAQENLRGLALTLTSAIENLAVNDPNLASLAKFRTNDIAYFALVDSQGRF
RFHSNPDLIGASLPEAERKAAPKLERSESRVALGTGEQAFLVTTPINLNGEILSLHLTLHTYRADAVVRSAKLNLTVMLA
LIATGWLLSLGLFRYARREELHQAEMTRKVNLAKLGEMGALLAHEIRNPLAGIKGFAQVIAKKPQEARNAAFAQNIVTEV
VRLEALVNDLLAYAAGDGAQRAPFDLGELIDNTLSLLAPEAVEREVSVCCSSTCSFFVMGNRDRIEQTLLNLGKNALQAM
DAGGVLEIACASAGGQAQISIKDTGQGIAEADLPKVFEPFFTTRARGTGLGLALCKKVIEEHGGTIGLESRIGEGTTVTL
ALPLLPADKEQT
>Mature_412_residues
MFRKATLIIGIFATCSLLWFTLYNYRQAGPIAQENLRGLALTLTSAIENLAVNDPNLASLAKFRTNDIAYFALVDSQGRF
RFHSNPDLIGASLPEAERKAAPKLERSESRVALGTGEQAFLVTTPINLNGEILSLHLTLHTYRADAVVRSAKLNLTVMLA
LIATGWLLSLGLFRYARREELHQAEMTRKVNLAKLGEMGALLAHEIRNPLAGIKGFAQVIAKKPQEARNAAFAQNIVTEV
VRLEALVNDLLAYAAGDGAQRAPFDLGELIDNTLSLLAPEAVEREVSVCCSSTCSFFVMGNRDRIEQTLLNLGKNALQAM
DAGGVLEIACASAGGQAQISIKDTGQGIAEADLPKVFEPFFTTRARGTGLGLALCKKVIEEHGGTIGLESRIGEGTTVTL
ALPLLPADKEQT

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=369, Percent_Identity=33.8753387533875, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1788549, Length=233, Percent_Identity=34.7639484978541, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1788393, Length=236, Percent_Identity=30.9322033898305, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789149, Length=246, Percent_Identity=28.8617886178862, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI87081816, Length=217, Percent_Identity=30.4147465437788, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786600, Length=240, Percent_Identity=27.5, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI145693157, Length=226, Percent_Identity=28.3185840707965, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1790300, Length=217, Percent_Identity=30.4147465437788, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788713, Length=232, Percent_Identity=27.5862068965517, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI48994928, Length=217, Percent_Identity=25.8064516129032, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786783, Length=236, Percent_Identity=25, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1790861, Length=201, Percent_Identity=28.8557213930348, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790346, Length=217, Percent_Identity=27.1889400921659, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786912, Length=214, Percent_Identity=25.7009345794392, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 44399; Mature: 44399

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRKATLIIGIFATCSLLWFTLYNYRQAGPIAQENLRGLALTLTSAIENLAVNDPNLASL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHCCCCCCHHHH
AKFRTNDIAYFALVDSQGRFRFHSNPDLIGASLPEAERKAAPKLERSESRVALGTGEQAF
HHHCCCCEEEEEEECCCCCEEECCCCCCCCCCCCHHHHHCCCCCCCCCCEEEECCCCEEE
LVTTPINLNGEILSLHLTLHTYRADAVVRSAKLNLTVMLALIATGWLLSLGLFRYARREE
EEEECCCCCCEEEEEEEEEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHQAEMTRKVNLAKLGEMGALLAHEIRNPLAGIKGFAQVIAKKPQEARNAAFAQNIVTEV
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VRLEALVNDLLAYAAGDGAQRAPFDLGELIDNTLSLLAPEAVEREVSVCCSSTCSFFVMG
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEC
NRDRIEQTLLNLGKNALQAMDAGGVLEIACASAGGQAQISIKDTGQGIAEADLPKVFEPF
CHHHHHHHHHHHCHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHH
FTTRARGTGLGLALCKKVIEEHGGTIGLESRIGEGTTVTLALPLLPADKEQT
HHHCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEEECCCCCCCC
>Mature Secondary Structure
MFRKATLIIGIFATCSLLWFTLYNYRQAGPIAQENLRGLALTLTSAIENLAVNDPNLASL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHCCCCCCHHHH
AKFRTNDIAYFALVDSQGRFRFHSNPDLIGASLPEAERKAAPKLERSESRVALGTGEQAF
HHHCCCCEEEEEEECCCCCEEECCCCCCCCCCCCHHHHHCCCCCCCCCCEEEECCCCEEE
LVTTPINLNGEILSLHLTLHTYRADAVVRSAKLNLTVMLALIATGWLLSLGLFRYARREE
EEEECCCCCCEEEEEEEEEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHQAEMTRKVNLAKLGEMGALLAHEIRNPLAGIKGFAQVIAKKPQEARNAAFAQNIVTEV
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VRLEALVNDLLAYAAGDGAQRAPFDLGELIDNTLSLLAPEAVEREVSVCCSSTCSFFVMG
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEC
NRDRIEQTLLNLGKNALQAMDAGGVLEIACASAGGQAQISIKDTGQGIAEADLPKVFEPF
CHHHHHHHHHHHCHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHH
FTTRARGTGLGLALCKKVIEEHGGTIGLESRIGEGTTVTLALPLLPADKEQT
HHHCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]