Click here to switch to the map view.

The map label for this gene is moeA-2 [H]

Identifier: 197118531

GI number: 197118531

Start: 2501800

End: 2503026

Strand: Direct

Name: moeA-2 [H]

Synonym: Gbem_2149

Alternate gene names: 197118531

Gene position: 2501800-2503026 (Clockwise)

Preceding gene: 197118530

Following gene: 197118532

Centisome position: 54.21

GC content: 62.75

Gene sequence:

>1227_bases
ATGATCAGCATCGAGCAAGCGCAACGCACCGTCTTCGAGCAGATAGTCCCGCTGGAAACGGAGAATGTTCCGGTCTCCCA
GGGGCTGAACCGGGTTACTCCGGAGAACCATCTCGCCCCCTGGGACATCCCCGCTGCTGACAACTCCGCCATGGACGGCT
TCGCTTTCCGCCACCAAAGCGCTGACCCGGTCGAGCTGAAGATAACCGGTTTTCTCCCTGCCGGGGAGGTCATAAACGAT
TCGGTTCCCGCCGGGTGCGCCATCAGGATCATGACCGGAGCCCCCATCCCTCCCGGATGCGACACCGTGGTTCCCATCGA
GGACGTAGAGTTGCAGGGGGATCTGGTCCGGCTTAAGTCGCCGGTGAAGGCTGGCAACCACGTCCGCAGGCGTGGCGAGG
ACATAGCCTGCGGCAACGTCGTCATACCTGCCGGCTCCGTGCTCCGCCCCCCGGAGATCGGCATGCTCTGCGCCATGGGA
AAAACGTCCTTGTCGCTTTACCGCAAAGCGAGAGTCGCCATCCTCGCCACCGGCGATGAACTTCTCGAACCAGGATCCCC
CCCCTCCCCCGGCAAGCTCATCGACAGCAACAGTTACAGCCTCGCCGCGCAGGTCGTCGAAGCGGGAGGCGAACCCATTG
TCCTCGGTATCGCCGCAGACACGCTTGAGGAGACCTGCGACAAGATCAAGGCGGGGTTGGACGCGGACATGCTGGTCGTC
TCGGGAGGGGTATCGGTCGGGGACAGGGATTACGTCAAGGCGGCCATAGAGCGGTTGGGAGGCGAGATCCAGTTCTGGAA
GGTCAACATGAAGCCGGGTAAGCCGTTGGCCTTCGCGCACCTGAAGGGTAGGCCTATCTTCGCCCTTCCCGGAAACCCTG
TTGCCGCCATGGTCTCCTTCGAACTCTTCGTGCGCCCCTCTATCTTGAAAGCGATGGGACATAGGCGCATCCTCCGTCCC
GTGGTGAACGCGCTCTTGCAGGAGCGCGCCAGCAACAACGGGGAGCGGCCGCACCTGGTGCGGGGCATCGTCTCCCGGCA
CGGAGAGCGCTATGAGGTCTCCACTACGGGCAATCAAAGCTCGGGAAGACTCTCCTCCTTGACCTTGGGCAACGGCTTGA
TGAAGCTGGCACCCCAGTCGACGCAGGAAGCGGGGAGCGAGGTAGAGGTCATGCTCCTGGACCGGTGGTTTGAACAGGGG
GGGCTCGAAGATGACGGCGTTGCCTGA

Upstream 100 bases:

>100_bases
TTCGGTGCGGTGAGGGGACCCGCTGCAGCAGGATTTCGAGGAGACGGCAAATGCCAAGTTCGCACAGCTTGGGTTTTGAA
ATACAAGAGGGAGCGCATCA

Downstream 100 bases:

>100_bases
AGTTCTCGACCTGGAACTCTATCCCGAGAGGGTGCGCCTGTGACCTTGCCGCTTCTGACATTGTTATTGGCTGCGGTGCT
CGTGGTCGCCGCCCTTTATT

Product: molybdopterin--molybdenum ligase

Products: NA

Alternate protein names: MPT Mo-transferase [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MISIEQAQRTVFEQIVPLETENVPVSQGLNRVTPENHLAPWDIPAADNSAMDGFAFRHQSADPVELKITGFLPAGEVIND
SVPAGCAIRIMTGAPIPPGCDTVVPIEDVELQGDLVRLKSPVKAGNHVRRRGEDIACGNVVIPAGSVLRPPEIGMLCAMG
KTSLSLYRKARVAILATGDELLEPGSPPSPGKLIDSNSYSLAAQVVEAGGEPIVLGIAADTLEETCDKIKAGLDADMLVV
SGGVSVGDRDYVKAAIERLGGEIQFWKVNMKPGKPLAFAHLKGRPIFALPGNPVAAMVSFELFVRPSILKAMGHRRILRP
VVNALLQERASNNGERPHLVRGIVSRHGERYEVSTTGNQSSGRLSSLTLGNGLMKLAPQSTQEAGSEVEVMLLDRWFEQG
GLEDDGVA

Sequences:

>Translated_408_residues
MISIEQAQRTVFEQIVPLETENVPVSQGLNRVTPENHLAPWDIPAADNSAMDGFAFRHQSADPVELKITGFLPAGEVIND
SVPAGCAIRIMTGAPIPPGCDTVVPIEDVELQGDLVRLKSPVKAGNHVRRRGEDIACGNVVIPAGSVLRPPEIGMLCAMG
KTSLSLYRKARVAILATGDELLEPGSPPSPGKLIDSNSYSLAAQVVEAGGEPIVLGIAADTLEETCDKIKAGLDADMLVV
SGGVSVGDRDYVKAAIERLGGEIQFWKVNMKPGKPLAFAHLKGRPIFALPGNPVAAMVSFELFVRPSILKAMGHRRILRP
VVNALLQERASNNGERPHLVRGIVSRHGERYEVSTTGNQSSGRLSSLTLGNGLMKLAPQSTQEAGSEVEVMLLDRWFEQG
GLEDDGVA
>Mature_408_residues
MISIEQAQRTVFEQIVPLETENVPVSQGLNRVTPENHLAPWDIPAADNSAMDGFAFRHQSADPVELKITGFLPAGEVIND
SVPAGCAIRIMTGAPIPPGCDTVVPIEDVELQGDLVRLKSPVKAGNHVRRRGEDIACGNVVIPAGSVLRPPEIGMLCAMG
KTSLSLYRKARVAILATGDELLEPGSPPSPGKLIDSNSYSLAAQVVEAGGEPIVLGIAADTLEETCDKIKAGLDADMLVV
SGGVSVGDRDYVKAAIERLGGEIQFWKVNMKPGKPLAFAHLKGRPIFALPGNPVAAMVSFELFVRPSILKAMGHRRILRP
VVNALLQERASNNGERPHLVRGIVSRHGERYEVSTTGNQSSGRLSSLTLGNGLMKLAPQSTQEAGSEVEVMLLDRWFEQG
GLEDDGVA

Specific function: Catalyzes the insertion of molybdate into adenylated molybdopterin with the concomitant release of AMP [H]

COG id: COG0303

COG function: function code H; Molybdopterin biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moeA family [H]

Homologues:

Organism=Homo sapiens, GI66932975, Length=419, Percent_Identity=36.7541766109785, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI10880983, Length=419, Percent_Identity=36.7541766109785, Blast_Score=207, Evalue=1e-53,
Organism=Escherichia coli, GI1787049, Length=381, Percent_Identity=38.5826771653543, Blast_Score=243, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI25147659, Length=400, Percent_Identity=31.5, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17562606, Length=290, Percent_Identity=27.5862068965517, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17136978, Length=386, Percent_Identity=33.419689119171, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24638841, Length=386, Percent_Identity=33.419689119171, Blast_Score=170, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020817
- InterPro:   IPR008284
- InterPro:   IPR005111
- InterPro:   IPR005110
- InterPro:   IPR001453 [H]

Pfam domain/function: PF00994 MoCF_biosynth; PF03454 MoeA_C; PF03453 MoeA_N [H]

EC number: NA

Molecular weight: Translated: 43487; Mature: 43487

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS01079 MOCF_BIOSYNTHESIS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISIEQAQRTVFEQIVPLETENVPVSQGLNRVTPENHLAPWDIPAADNSAMDGFAFRHQS
CCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCC
ADPVELKITGFLPAGEVINDSVPAGCAIRIMTGAPIPPGCDTVVPIEDVELQGDLVRLKS
CCCEEEEEEECCCCCHHHCCCCCCCEEEEEEECCCCCCCCCCEECCCCCCCCCCCEECCC
PVKAGNHVRRRGEDIACGNVVIPAGSVLRPPEIGMLCAMGKTSLSLYRKARVAILATGDE
CCCCCHHHHHCCCCEECCCEEECCCCCCCCCCCCEEEECCCHHHHHHHHEEEEEEECCHH
LLEPGSPPSPGKLIDSNSYSLAAQVVEAGGEPIVLGIAADTLEETCDKIKAGLDADMLVV
HHCCCCCCCCCCEECCCCHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHCCCCCEEEE
SGGVSVGDRDYVKAAIERLGGEIQFWKVNMKPGKPLAFAHLKGRPIFALPGNPVAAMVSF
ECCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEECCCEEEEECCCCEEEEEEE
ELFVRPSILKAMGHRRILRPVVNALLQERASNNGERPHLVRGIVSRHGERYEVSTTGNQS
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEECCCCC
SGRLSSLTLGNGLMKLAPQSTQEAGSEVEVMLLDRWFEQGGLEDDGVA
CCCEEEEEECCCHHCCCCCCHHHCCCCEEEEEHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MISIEQAQRTVFEQIVPLETENVPVSQGLNRVTPENHLAPWDIPAADNSAMDGFAFRHQS
CCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCC
ADPVELKITGFLPAGEVINDSVPAGCAIRIMTGAPIPPGCDTVVPIEDVELQGDLVRLKS
CCCEEEEEEECCCCCHHHCCCCCCCEEEEEEECCCCCCCCCCEECCCCCCCCCCCEECCC
PVKAGNHVRRRGEDIACGNVVIPAGSVLRPPEIGMLCAMGKTSLSLYRKARVAILATGDE
CCCCCHHHHHCCCCEECCCEEECCCCCCCCCCCCEEEECCCHHHHHHHHEEEEEEECCHH
LLEPGSPPSPGKLIDSNSYSLAAQVVEAGGEPIVLGIAADTLEETCDKIKAGLDADMLVV
HHCCCCCCCCCCEECCCCHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHCCCCCEEEE
SGGVSVGDRDYVKAAIERLGGEIQFWKVNMKPGKPLAFAHLKGRPIFALPGNPVAAMVSF
ECCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEECCCEEEEECCCCEEEEEEE
ELFVRPSILKAMGHRRILRPVVNALLQERASNNGERPHLVRGIVSRHGERYEVSTTGNQS
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEECCCCC
SGRLSSLTLGNGLMKLAPQSTQEAGSEVEVMLLDRWFEQGGLEDDGVA
CCCEEEEEECCCHHCCCCCCHHHCCCCEEEEEHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3045084; 8905232; 9278503; 9515915 [H]