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The map label for this gene is nifB [H]

Identifier: 197118465

GI number: 197118465

Start: 2408327

End: 2409184

Strand: Direct

Name: nifB [H]

Synonym: Gbem_2083

Alternate gene names: 197118465

Gene position: 2408327-2409184 (Clockwise)

Preceding gene: 197118463

Following gene: 197118466

Centisome position: 52.18

GC content: 59.91

Gene sequence:

>858_bases
ATGGCGACATCGTGCGAGAAGATGAAGAAGATCCAGGGGCATCCCTGCTTTGGCGGCAACCACCATAAAAACGGGCGGAT
GCACCTCGCGGTGGCGCCCAAGTGCAACATCAAATGCGGCTATTGCACCCGGCGTCACGACTGCGCCAACGAATCGCGCC
CGGGCGTCACGAGCCGCCTGCTCTCGCCGGACGAGGCGATCGTCAGGGTGAGGGAGGTGATGGCAAGCCCCATCACCGGC
CCCATCATCAAGGTCATCGGCATTGCCGGGCCGGGGGATCCGCTCTTCAACGAAGAGACCTTCGACACCTTTCGTTACGT
GGACCGGGAGTTTCCGCAGTTGATCAAGTGTCTGAGCACCAACGGGCTGCTGCTACCCGACAAGATGCCAGTGCTTAAGG
AGATCGGACTGCACAGCCTCACCGTCACTCTCAACGCGTTGGACCCGAAGGTCGGCGGCAAGATCTACTCCCACGTCTTC
TATAAGGGGACCAAATACACAGGCGAGGAGGGGGCGGAGATCCTGGTTCGTAACCAGTTGGAAGGGATCCGCCAGGCGGC
GGAACTGGGGCTGACCATCAAGATAAACACGGTGCTGATACCCGGTGTGAACGACGAGCAGATCCCGCTCATCTCGAAGA
AGGTTAAGGAACTTGGGGCCTTCGTGATGAACATCATGCCGCTCATTCCGCAGGCCGATTTCGCCCATATCGAGCCTCCT
TCAGCAGACCGGCTGGATGCGCTACGCCAGGATAACGAGAAGACCATTGGACAGTTCAAACACTGCAAACAGTGCCGTGC
CGACGCGGTGGGGCTGATCTGCGAAAACCTGCAGCTCGCCATCGCCCCGGCGGCGTAG

Upstream 100 bases:

>100_bases
CTAACATAAACGGCAACGATGCCCGGCGCGGATAGAACATATCCCGCCGGGCATTTTTCGTTTTCAGGCACGGCCTGAAG
CAAGGATCAAGGAGGATGAC

Downstream 100 bases:

>100_bases
CAGATGATCGAACCGTTCGTCGAGTCCGACATACCTTCCTTCATAGCTCTAGCCAGGGAGGAAGGGTGGGTCTGCGGGGC
CTGGGAGTTTCAGTTCCTGC

Product: radical SAM protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MATSCEKMKKIQGHPCFGGNHHKNGRMHLAVAPKCNIKCGYCTRRHDCANESRPGVTSRLLSPDEAIVRVREVMASPITG
PIIKVIGIAGPGDPLFNEETFDTFRYVDREFPQLIKCLSTNGLLLPDKMPVLKEIGLHSLTVTLNALDPKVGGKIYSHVF
YKGTKYTGEEGAEILVRNQLEGIRQAAELGLTIKINTVLIPGVNDEQIPLISKKVKELGAFVMNIMPLIPQADFAHIEPP
SADRLDALRQDNEKTIGQFKHCKQCRADAVGLICENLQLAIAPAA

Sequences:

>Translated_285_residues
MATSCEKMKKIQGHPCFGGNHHKNGRMHLAVAPKCNIKCGYCTRRHDCANESRPGVTSRLLSPDEAIVRVREVMASPITG
PIIKVIGIAGPGDPLFNEETFDTFRYVDREFPQLIKCLSTNGLLLPDKMPVLKEIGLHSLTVTLNALDPKVGGKIYSHVF
YKGTKYTGEEGAEILVRNQLEGIRQAAELGLTIKINTVLIPGVNDEQIPLISKKVKELGAFVMNIMPLIPQADFAHIEPP
SADRLDALRQDNEKTIGQFKHCKQCRADAVGLICENLQLAIAPAA
>Mature_284_residues
ATSCEKMKKIQGHPCFGGNHHKNGRMHLAVAPKCNIKCGYCTRRHDCANESRPGVTSRLLSPDEAIVRVREVMASPITGP
IIKVIGIAGPGDPLFNEETFDTFRYVDREFPQLIKCLSTNGLLLPDKMPVLKEIGLHSLTVTLNALDPKVGGKIYSHVFY
KGTKYTGEEGAEILVRNQLEGIRQAAELGLTIKINTVLIPGVNDEQIPLISKKVKELGAFVMNIMPLIPQADFAHIEPPS
ADRLDALRQDNEKTIGQFKHCKQCRADAVGLICENLQLAIAPAA

Specific function: Probably involved in the synthesis of the Fe-Mo cofactor [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaA/nifB/pqqE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR003731
- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR005980
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02579 Nitro_FeMo-Co; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 31270; Mature: 31139

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATSCEKMKKIQGHPCFGGNHHKNGRMHLAVAPKCNIKCGYCTRRHDCANESRPGVTSRL
CCCCHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCEECCCCCCCCCCCCCCCCHHHHH
LSPDEAIVRVREVMASPITGPIIKVIGIAGPGDPLFNEETFDTFRYVDREFPQLIKCLST
CCCHHHHHHHHHHHHCCCCCCHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
NGLLLPDKMPVLKEIGLHSLTVTLNALDPKVGGKIYSHVFYKGTKYTGEEGAEILVRNQL
CCEECCCCCHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHH
EGIRQAAELGLTIKINTVLIPGVNDEQIPLISKKVKELGAFVMNIMPLIPQADFAHIEPP
HHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
SADRLDALRQDNEKTIGQFKHCKQCRADAVGLICENLQLAIAPAA
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
>Mature Secondary Structure 
ATSCEKMKKIQGHPCFGGNHHKNGRMHLAVAPKCNIKCGYCTRRHDCANESRPGVTSRL
CCCHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCEECCCCCCCCCCCCCCCCHHHHH
LSPDEAIVRVREVMASPITGPIIKVIGIAGPGDPLFNEETFDTFRYVDREFPQLIKCLST
CCCHHHHHHHHHHHHCCCCCCHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
NGLLLPDKMPVLKEIGLHSLTVTLNALDPKVGGKIYSHVFYKGTKYTGEEGAEILVRNQL
CCEECCCCCHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHH
EGIRQAAELGLTIKINTVLIPGVNDEQIPLISKKVKELGAFVMNIMPLIPQADFAHIEPP
HHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
SADRLDALRQDNEKTIGQFKHCKQCRADAVGLICENLQLAIAPAA
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3062178; 3029020 [H]