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The map label for this gene is pleD [H]

Identifier: 197118450

GI number: 197118450

Start: 2386746

End: 2387648

Strand: Direct

Name: pleD [H]

Synonym: Gbem_2068

Alternate gene names: 197118450

Gene position: 2386746-2387648 (Clockwise)

Preceding gene: 197118449

Following gene: 197118451

Centisome position: 51.72

GC content: 60.35

Gene sequence:

>903_bases
GTGTATCTGAGCAAGCAGTCCTACAAAAAGATCATCGACGACCTGCGCGACGGGCTCTACATCGTGGATCGCGACCGGAA
GGTAGTCTTTTGGAACCGCGCAGCTGAGCGCATCTCCGGGTTTAGCGCCGAAGAGGTGATTTCCAAACGCTGCGCCGACA
ACATCCTTTGCCATGTCGACGACATCGGGCAAAACCTCTGCAACAGTTCCTGTCCGCTTGCCGCGACCATCGATTGCGGC
ATGGCCCAGGAGGCCGAGCTCTTCCTGCACCACAAGAACGGGCACCGGGTCCCGGTTACGGTGCAGGTGGTCCCCTTGAC
CGACGACAAGGGAGAGGTCATCGGCGCCGTGGAGATGTTCTCGGACGGCAGCAACAAGGCAGCGAACGAACTGCGACTGC
AGGAACTGGAGAAGCTCGCGCTGCTGGACGAACTGACCCAACTCGCCAACCGCCGCTACCTGGAGCGGGAACTCGAAGCG
CGCCTCGAAGAGATGAGCCGCTACGACATCCCTTTCGGGGTCCTGTTCATGGATCTCGACGATTTCAAGAAAGTGAACGA
CGACTACGGCCACGAAACCGGAGACCGCGTGCTCAGCTTCGTGGCTGGAACATTGAGCGCCAACTCCCGTCCCTTCGACC
TCTACGGTCGCTGGGGAGGGGAGGAATTCGTAGGGATCGTGCGCAACGTCACCGCCGCCAACCTGAAGCACATGGGACAG
CGCCTGCGCAAGCTGGTGGAGAAGTCCTTCCTGCTGGTAGGGGAGGAGAGGCTCAGGGTAACCATCTCCATCGGCGCTAC
CATGGCGCTTCCGGAAGACACGCTTCAGTCTTTGCTGCAGCGCGCCGACCTGCTCATGTACCAGAGCAAGAGCGCCGGCA
AAAACCGTCTGACTATCGGCTGA

Upstream 100 bases:

>100_bases
TGACAACTTACAGCAGTAGCACTATACTCATTAGCCAGTCGACGTATCTACTTTAATTTCACTAGACAGGCATCATGTCA
TCAGCCAGGGAGTTCCGTCC

Downstream 100 bases:

>100_bases
CATCCCGACCGGGCTAGCGTCGCAGACAAAGAGCCGGCTCCCGGGGCGGCTGCTTCGATCTTTTCCTTAGCATCCGAGGC
TTGCGCTATGAAATGTGCTG

Product: PAS domain-containing sensor diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MYLSKQSYKKIIDDLRDGLYIVDRDRKVVFWNRAAERISGFSAEEVISKRCADNILCHVDDIGQNLCNSSCPLAATIDCG
MAQEAELFLHHKNGHRVPVTVQVVPLTDDKGEVIGAVEMFSDGSNKAANELRLQELEKLALLDELTQLANRRYLERELEA
RLEEMSRYDIPFGVLFMDLDDFKKVNDDYGHETGDRVLSFVAGTLSANSRPFDLYGRWGGEEFVGIVRNVTAANLKHMGQ
RLRKLVEKSFLLVGEERLRVTISIGATMALPEDTLQSLLQRADLLMYQSKSAGKNRLTIG

Sequences:

>Translated_300_residues
MYLSKQSYKKIIDDLRDGLYIVDRDRKVVFWNRAAERISGFSAEEVISKRCADNILCHVDDIGQNLCNSSCPLAATIDCG
MAQEAELFLHHKNGHRVPVTVQVVPLTDDKGEVIGAVEMFSDGSNKAANELRLQELEKLALLDELTQLANRRYLERELEA
RLEEMSRYDIPFGVLFMDLDDFKKVNDDYGHETGDRVLSFVAGTLSANSRPFDLYGRWGGEEFVGIVRNVTAANLKHMGQ
RLRKLVEKSFLLVGEERLRVTISIGATMALPEDTLQSLLQRADLLMYQSKSAGKNRLTIG
>Mature_300_residues
MYLSKQSYKKIIDDLRDGLYIVDRDRKVVFWNRAAERISGFSAEEVISKRCADNILCHVDDIGQNLCNSSCPLAATIDCG
MAQEAELFLHHKNGHRVPVTVQVVPLTDDKGEVIGAVEMFSDGSNKAANELRLQELEKLALLDELTQLANRRYLERELEA
RLEEMSRYDIPFGVLFMDLDDFKKVNDDYGHETGDRVLSFVAGTLSANSRPFDLYGRWGGEEFVGIVRNVTAANLKHMGQ
RLRKLVEKSFLLVGEERLRVTISIGATMALPEDTLQSLLQRADLLMYQSKSAGKNRLTIG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1788381, Length=302, Percent_Identity=26.8211920529801, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI145693134, Length=159, Percent_Identity=33.9622641509434, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787816, Length=158, Percent_Identity=34.8101265822785, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87082007, Length=170, Percent_Identity=37.6470588235294, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787262, Length=174, Percent_Identity=34.4827586206897, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI87081881, Length=189, Percent_Identity=31.7460317460317, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787802, Length=178, Percent_Identity=33.1460674157303, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788085, Length=173, Percent_Identity=33.5260115606936, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786584, Length=164, Percent_Identity=28.6585365853659, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787541, Length=295, Percent_Identity=24.406779661017, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788956, Length=166, Percent_Identity=31.9277108433735, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081977, Length=149, Percent_Identity=30.8724832214765, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87081974, Length=163, Percent_Identity=26.3803680981595, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787056, Length=175, Percent_Identity=30.8571428571429, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 33817; Mature: 33817

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLSKQSYKKIIDDLRDGLYIVDRDRKVVFWNRAAERISGFSAEEVISKRCADNILCHVD
CCCCHHHHHHHHHHHHCCEEEEECCCEEEEECHHHHHHCCCCHHHHHHHHHHHCCEEEHH
DIGQNLCNSSCPLAATIDCGMAQEAELFLHHKNGHRVPVTVQVVPLTDDKGEVIGAVEMF
HHHHHHCCCCCCEEEEECCCCCCCCEEEEECCCCCEEEEEEEEEEECCCCCCEEEHHHHH
SDGSNKAANELRLQELEKLALLDELTQLANRRYLERELEARLEEMSRYDIPFGVLFMDLD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHH
DFKKVNDDYGHETGDRVLSFVAGTLSANSRPFDLYGRWGGEEFVGIVRNVTAANLKHMGQ
HHHHCCHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
RLRKLVEKSFLLVGEERLRVTISIGATMALPEDTLQSLLQRADLLMYQSKSAGKNRLTIG
HHHHHHHHHHHEECCCEEEEEEEECCEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MYLSKQSYKKIIDDLRDGLYIVDRDRKVVFWNRAAERISGFSAEEVISKRCADNILCHVD
CCCCHHHHHHHHHHHHCCEEEEECCCEEEEECHHHHHHCCCCHHHHHHHHHHHCCEEEHH
DIGQNLCNSSCPLAATIDCGMAQEAELFLHHKNGHRVPVTVQVVPLTDDKGEVIGAVEMF
HHHHHHCCCCCCEEEEECCCCCCCCEEEEECCCCCEEEEEEEEEEECCCCCCEEEHHHHH
SDGSNKAANELRLQELEKLALLDELTQLANRRYLERELEARLEEMSRYDIPFGVLFMDLD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHH
DFKKVNDDYGHETGDRVLSFVAGTLSANSRPFDLYGRWGGEEFVGIVRNVTAANLKHMGQ
HHHHCCHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
RLRKLVEKSFLLVGEERLRVTISIGATMALPEDTLQSLLQRADLLMYQSKSAGKNRLTIG
HHHHHHHHHHHEECCCEEEEEEEECCEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA