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The map label for this gene is prfA [H]

Identifier: 197118419

GI number: 197118419

Start: 2344820

End: 2345137

Strand: Direct

Name: prfA [H]

Synonym: Gbem_2037

Alternate gene names: 197118419

Gene position: 2344820-2345137 (Clockwise)

Preceding gene: 197118416

Following gene: 197118421

Centisome position: 50.81

GC content: 61.32

Gene sequence:

>318_bases
GTGGAGATAAGGGACGTCGATATAAAAGTGGAATTTTACCGTGCTTCGGGACCGGGCGGGCAGCACCGCAATACGACCGA
TTCGGCGGTGAGGATCCGGCACCTGCCGACCGGGGTGGTGGCGCAGGCGAGCGAGAGCCGGTCGCAGTTCGAAAACCGGG
AGAAGGCGATGGAGCGTTTGGCCGAGTTGCTGCGCCGGCGGGAGCAAAAAGCGAAGAAACGGGTCGCGACCAAGGTGCCG
CGCCGCGCCAAGGAGAAGCGCCTGAACCAGAAAAAGGCGCATTCCGATAAAAAGAAGCTACGCTCCACCCTCGATTGA

Upstream 100 bases:

>100_bases
AGGGCTGCCGGAAAAGCTGTGCTATACTCGCCGGCGGTTTCCAGGGGACTGGCTCCGCAAGGTGCCTGTCCCCCTTGACT
CTAAATGGGGCAGGTGTTCG

Downstream 100 bases:

>100_bases
GGCTTTGGCGTACATTTGCATCATGATCTCCTTGAAGGCGATCGCCGCAGGTGACAACTCCCTCCCTTTCCTGGTAGCCA
GGAAGAAATCCCGCTTGATC

Product: hydrolase

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 105; Mature: 105

Protein sequence:

>105_residues
MEIRDVDIKVEFYRASGPGGQHRNTTDSAVRIRHLPTGVVAQASESRSQFENREKAMERLAELLRRREQKAKKRVATKVP
RRAKEKRLNQKKAHSDKKKLRSTLD

Sequences:

>Translated_105_residues
MEIRDVDIKVEFYRASGPGGQHRNTTDSAVRIRHLPTGVVAQASESRSQFENREKAMERLAELLRRREQKAKKRVATKVP
RRAKEKRLNQKKAHSDKKKLRSTLD
>Mature_105_residues
MEIRDVDIKVEFYRASGPGGQHRNTTDSAVRIRHLPTGVVAQASESRSQFENREKAMERLAELLRRREQKAKKRVATKVP
RRAKEKRLNQKKAHSDKKKLRSTLD

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=54, Percent_Identity=61.1111111111111, Blast_Score=74, Evalue=2e-14,
Organism=Homo sapiens, GI34577120, Length=90, Percent_Identity=38.8888888888889, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787462, Length=79, Percent_Identity=44.3037974683544, Blast_Score=75, Evalue=7e-16,
Organism=Escherichia coli, GI2367172, Length=73, Percent_Identity=45.2054794520548, Blast_Score=74, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321295, Length=54, Percent_Identity=53.7037037037037, Blast_Score=65, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19921226, Length=58, Percent_Identity=63.7931034482759, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 12206; Mature: 12206

Theoretical pI: Translated: 11.60; Mature: 11.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIRDVDIKVEFYRASGPGGQHRNTTDSAVRIRHLPTGVVAQASESRSQFENREKAMERL
CCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
AELLRRREQKAKKRVATKVPRRAKEKRLNQKKAHSDKKKLRSTLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEIRDVDIKVEFYRASGPGGQHRNTTDSAVRIRHLPTGVVAQASESRSQFENREKAMERL
CCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
AELLRRREQKAKKRVATKVPRRAKEKRLNQKKAHSDKKKLRSTLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA