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The map label for this gene is cheR-8 [H]

Identifier: 197118412

GI number: 197118412

Start: 2339420

End: 2340865

Strand: Reverse

Name: cheR-8 [H]

Synonym: Gbem_2030

Alternate gene names: 197118412

Gene position: 2340865-2339420 (Counterclockwise)

Preceding gene: 197118413

Following gene: 197118411

Centisome position: 50.72

GC content: 64.59

Gene sequence:

>1446_bases
GTGAGCGCGCACAGCAACGAGGCAATGCATAAGTTCGCCCACATGGTGACGACAAAGCTGGGGCTCCACTTTCCGCCCGA
GCGTCTGGCTGAGTTGGCCCGGAAGATGGCGCCGCTGGCGAAGGATGCGGGCTGGGAGGAACTGGACGGCTACCTGCACT
GGCTCATGTCGCAGCCGCTCTCCCTGGAGCAGACCAAGGCGCTGGCCCTGGCGCTCACCATCGGCGAGACCTATTTTCTG
CGCGACCCCAAGAGCTACCGGGCCTTCCAGCAGCATCTGCTCCCTAAGCTCTTGGCGGCCAAAGGTTCGGACAAAAGCCT
CAAGATCTGGAGCGCCGGCTGTTCCTCCGGAGAGGAGCCGTACTCGATCGCCATACTGCTTACCCGTGCCCTGGCCGACC
TGGCCGACTGGAAGGTGACCCTGCTCGGAACCGACATCAACCCCCAGGCGTTGGAGCGGGCCCGATGCGGAACCTACAGC
AAATGGTCCTTCCGGAACGCGCCGGGGTGGCTCATGAATTACTTCACCCAGCTCCCTGACGGGAACTACCAGATCGAGCC
GCATATCCGCAAGATGGTGCGCTTCGCGCATCTGAACTTGGTCGATCCCGGGGAAAAGACATGGTCGCAGGCCCAAGGGA
TGGACTTCATCTTCTGCCGCAACGTGATGCTTTACTTTCATCCGGAGCAGATACGGGAGACGGTCGCGAGACTGCACGCA
GCTCTCAACGACGGCGGCTGGCTCTTCCTGGGGCCGACGGAGGTGGACCACCAGGCGCTTGAGGGTTTTACCTGCCTGCA
CTGCGACGGCACCCTGGTGCTGCAAAAGCGCCCTCTCCCGGCCAAAGCGCAACTCTCGGCGCTGTCCGGACCTGCCGTGG
CCGCCCCCTCCCCTTTTTCCGCGCAGCTTTGGTCCGAAGGCGCGGAGCCGGGAAGCGCGCGGAAAGACGAGGCAGCCGCC
GAAGCAGAGTCGCCCCTGGAGCGGGCCTTTGCGCTTTACAGCGCCGGGAATTACGAGGAAGCGGCGCGGATGGCGATGAG
CACCCCGGCAAGCGCAGACAAGGCCGAAGCGCTGGCGCTCGCGGCGCGCTCCTATGCCAACATCGGCCGTTTCGACCCCG
CTCGCGACTGCTGCGAAGCCGCCGTGGCGCTGGACCGGCTCAGCGCACCCAACCATTACCTCCTGTCCATCATCCTGGAG
CAGCAGGGGGACGGCGAGGGAGCGGTGAGATCGCTCAGAAACGCGCTCTACGTCGATCACGACTTCCTGCTGGGTTGGTT
TGCCCTGGGAAACCTCTGCCGGCAGCGCGGGGAACTAAGGGAAGCGGAACAGAGCTTCGCCAACGCGCTGCGCCTTTTGC
AGCGGCGCGACCCCCACGAGGTGCTGCCGGAGGCAGAGGGGATGACTGCGGGAAGGCTGATGCAGCTGATCAGCGACATC
GCCTAA

Upstream 100 bases:

>100_bases
TCTCCGGAGTGACGAGGAACGAGGGTGGGCTGGTGCTGATCCACGACCTCGACACCCTGCTCTTCCCCGACGAGGAGCAG
CAGATCGCAAAGGCCCTGAC

Downstream 100 bases:

>100_bases
CGAAGGTGCGGCAAAACAGGGCCGAGAGCTTCAAGTAGTCAAGGAGAAACACCCATGGCAGCGGGCAAGATAGACTGGGA
CGACCTGATCAAACGCCAGG

Product: chemotaxis protein-glutamate methyltransferase CheR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MSAHSNEAMHKFAHMVTTKLGLHFPPERLAELARKMAPLAKDAGWEELDGYLHWLMSQPLSLEQTKALALALTIGETYFL
RDPKSYRAFQQHLLPKLLAAKGSDKSLKIWSAGCSSGEEPYSIAILLTRALADLADWKVTLLGTDINPQALERARCGTYS
KWSFRNAPGWLMNYFTQLPDGNYQIEPHIRKMVRFAHLNLVDPGEKTWSQAQGMDFIFCRNVMLYFHPEQIRETVARLHA
ALNDGGWLFLGPTEVDHQALEGFTCLHCDGTLVLQKRPLPAKAQLSALSGPAVAAPSPFSAQLWSEGAEPGSARKDEAAA
EAESPLERAFALYSAGNYEEAARMAMSTPASADKAEALALAARSYANIGRFDPARDCCEAAVALDRLSAPNHYLLSIILE
QQGDGEGAVRSLRNALYVDHDFLLGWFALGNLCRQRGELREAEQSFANALRLLQRRDPHEVLPEAEGMTAGRLMQLISDI
A

Sequences:

>Translated_481_residues
MSAHSNEAMHKFAHMVTTKLGLHFPPERLAELARKMAPLAKDAGWEELDGYLHWLMSQPLSLEQTKALALALTIGETYFL
RDPKSYRAFQQHLLPKLLAAKGSDKSLKIWSAGCSSGEEPYSIAILLTRALADLADWKVTLLGTDINPQALERARCGTYS
KWSFRNAPGWLMNYFTQLPDGNYQIEPHIRKMVRFAHLNLVDPGEKTWSQAQGMDFIFCRNVMLYFHPEQIRETVARLHA
ALNDGGWLFLGPTEVDHQALEGFTCLHCDGTLVLQKRPLPAKAQLSALSGPAVAAPSPFSAQLWSEGAEPGSARKDEAAA
EAESPLERAFALYSAGNYEEAARMAMSTPASADKAEALALAARSYANIGRFDPARDCCEAAVALDRLSAPNHYLLSIILE
QQGDGEGAVRSLRNALYVDHDFLLGWFALGNLCRQRGELREAEQSFANALRLLQRRDPHEVLPEAEGMTAGRLMQLISDI
A
>Mature_480_residues
SAHSNEAMHKFAHMVTTKLGLHFPPERLAELARKMAPLAKDAGWEELDGYLHWLMSQPLSLEQTKALALALTIGETYFLR
DPKSYRAFQQHLLPKLLAAKGSDKSLKIWSAGCSSGEEPYSIAILLTRALADLADWKVTLLGTDINPQALERARCGTYSK
WSFRNAPGWLMNYFTQLPDGNYQIEPHIRKMVRFAHLNLVDPGEKTWSQAQGMDFIFCRNVMLYFHPEQIRETVARLHAA
LNDGGWLFLGPTEVDHQALEGFTCLHCDGTLVLQKRPLPAKAQLSALSGPAVAAPSPFSAQLWSEGAEPGSARKDEAAAE
AESPLERAFALYSAGNYEEAARMAMSTPASADKAEALALAARSYANIGRFDPARDCCEAAVALDRLSAPNHYLLSIILEQ
QGDGEGAVRSLRNALYVDHDFLLGWFALGNLCRQRGELREAEQSFANALRLLQRRDPHEVLPEAEGMTAGRLMQLISDIA

Specific function: Methylation of the membrane-bound methyl-accepting chemotaxis proteins (MCP) to form gamma-glutamyl methyl ester residues in MCP [H]

COG id: COG1352

COG function: function code NT; Methylase of chemotaxis methyl-accepting proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 cheR-type methyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1788193, Length=257, Percent_Identity=29.1828793774319, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022642
- InterPro:   IPR000780
- InterPro:   IPR022641 [H]

Pfam domain/function: PF01739 CheR; PF03705 CheR_N [H]

EC number: =2.1.1.80 [H]

Molecular weight: Translated: 53186; Mature: 53055

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS50293 TPR_REGION ; PS50123 CHER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAHSNEAMHKFAHMVTTKLGLHFPPERLAELARKMAPLAKDAGWEELDGYLHWLMSQPL
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
SLEQTKALALALTIGETYFLRDPKSYRAFQQHLLPKLLAAKGSDKSLKIWSAGCSSGEEP
CHHHHHHEEEEEEECCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCC
YSIAILLTRALADLADWKVTLLGTDINPQALERARCGTYSKWSFRNAPGWLMNYFTQLPD
HHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCC
GNYQIEPHIRKMVRFAHLNLVDPGEKTWSQAQGMDFIFCRNVMLYFHPEQIRETVARLHA
CCEEECHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEHHCCCHHEECHHHHHHHHHHHHH
ALNDGGWLFLGPTEVDHQALEGFTCLHCDGTLVLQKRPLPAKAQLSALSGPAVAAPSPFS
HHCCCCEEEECCCHHHHHHHCCEEEEECCCEEEEECCCCCCHHHHHHCCCCCCCCCCCCH
AQLWSEGAEPGSARKDEAAAEAESPLERAFALYSAGNYEEAARMAMSTPASADKAEALAL
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHH
AARSYANIGRFDPARDCCEAAVALDRLSAPNHYLLSIILEQQGDGEGAVRSLRNALYVDH
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHEEHEEECCCCCCHHHHHHHHHHHHCCH
DFLLGWFALGNLCRQRGELREAEQSFANALRLLQRRDPHEVLPEAEGMTAGRLMQLISDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHC
A
C
>Mature Secondary Structure 
SAHSNEAMHKFAHMVTTKLGLHFPPERLAELARKMAPLAKDAGWEELDGYLHWLMSQPL
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
SLEQTKALALALTIGETYFLRDPKSYRAFQQHLLPKLLAAKGSDKSLKIWSAGCSSGEEP
CHHHHHHEEEEEEECCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCC
YSIAILLTRALADLADWKVTLLGTDINPQALERARCGTYSKWSFRNAPGWLMNYFTQLPD
HHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCC
GNYQIEPHIRKMVRFAHLNLVDPGEKTWSQAQGMDFIFCRNVMLYFHPEQIRETVARLHA
CCEEECHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEHHCCCHHEECHHHHHHHHHHHHH
ALNDGGWLFLGPTEVDHQALEGFTCLHCDGTLVLQKRPLPAKAQLSALSGPAVAAPSPFS
HHCCCCEEEECCCHHHHHHHCCEEEEECCCEEEEECCCCCCHHHHHHCCCCCCCCCCCCH
AQLWSEGAEPGSARKDEAAAEAESPLERAFALYSAGNYEEAARMAMSTPASADKAEALAL
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHH
AARSYANIGRFDPARDCCEAAVALDRLSAPNHYLLSIILEQQGDGEGAVRSLRNALYVDH
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHEEHEEECCCCCCHHHHHHHHHHHHCCH
DFLLGWFALGNLCRQRGELREAEQSFANALRLLQRRDPHEVLPEAEGMTAGRLMQLISDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]