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The map label for this gene is lhgO [H]

Identifier: 197118404

GI number: 197118404

Start: 2327581

End: 2328780

Strand: Direct

Name: lhgO [H]

Synonym: Gbem_2022

Alternate gene names: 197118404

Gene position: 2327581-2328780 (Clockwise)

Preceding gene: 308535281

Following gene: 197118414

Centisome position: 50.43

GC content: 60.83

Gene sequence:

>1200_bases
ATGAGCTTCGACAACGCCGGAATACTGATCGTCGGTGGCGGCATTATCGGCCTTACCATCGCCCGCGAACTGGTGAAGCA
GGGCCACGGCGACATTGTCATCATCGAGAAGGAACCGGAACTGGGCGTCCACGCCTCGGGCCGCAACAGCGGCGTGCTCC
ATGCCGGCATCTACTATTCACCGGACAGCCTCAAGGCCAAATCCTGCTTGAACGGCAATTTCCTGATGCGGGAATACTGC
AAGGAAAAGGGGCTGCCGCTGCTGGAAAGCGGCAAGGTCATCGTCACCCGCACCGCGGCCGAACTCCCGGTGCTGGACGA
ACTGCACCGGCGCGCGACTGCCAACGGCGCCAAGGTGGAGATGATCGACGAGAAGCAACTGGCCGCAATCGAGCCGAACG
CCCGGACGGTGGAGCGCGCGCTCTTCTCGCATTACACGGCGGTGGTGGACCCGAAAGCGGTGCTGAAGAGCCTGAAAAAG
GACCTGGAACGGACCGGAAGGGTGAGGCTTCACCTCGGCTGCAAAATGCTAGGCCTTAAGGGAAGCTCCACGGTGGTGAC
CAACAAGGGGGATATAAGCTTCGAGAGGTTCATCAACGCAGCCGGCGCCTACTGCAACAAGGTGGCGGGCTTCTTCGGGG
TGGGTTCCAAATACCGGTTGATCCCCTTCAAGGGGGTGTACCGGCTGCTGAAAAAGGATGCTCCCTTTACCGTCAACTCC
AACATCTATCCGGTGCCCGACATCCGGAACCCCTTTCTGGGGATCCACTTCACCCGCAGCGTCCACGGCGACGTCTATCT
GGGACCGACGGCCATTCCCGCTTTCGGGCGTGAGAACTACGGCATCCTGTCTGGCATCGACGCCGAGGCCTTCAGCATCG
CCTGGCAGGACCTGGTCCTTTTCCTCCTCAACAGGCCTTTCCGTAACGTCGCGCTCTCGGAGCCGCTCAAGTATTTCCCC
TCTTACTTCTTCCGCGACGCCGCAAGGCTGGTGAAGGAGCTGGCCCCCTCCGACGTGGTGCATGCTTCCAAGGTGGGGAT
ACGTCCGCAGCTGGTGGACTGGGAGAAGAAGGAGCTGGTGATGGATTTCCTGGTGGTGGCTGACGGCTCTTCGCTCCACG
TGCTGAACCCGATTTCCCCCGCTTTCACCTCGTCGATGGATCTGGCGCAGGGGATGGTGGCGGAGCATTTCTCTTCCTGA

Upstream 100 bases:

>100_bases
GCTTTTCGTTTTTCCTGCTTCTGTTTTTTCTCTGTGCTGCCTCTGTGTCCTCCGTGTCCTCTGTGGCGAAGATCTTAAGG
TTTTTCTAGGAGGTTACTTC

Downstream 100 bases:

>100_bases
AGCCGCTCGTTCGCCTTCTCTCCCCCTCCCGCAAGGGGAGGGGGGACTTACCCTTACTTCCCCTTTTACGACGCCTGCAG
CTTCAGCCGCTTGTTCAGGG

Product: malate:quinone oxidoreductase superfamily protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MSFDNAGILIVGGGIIGLTIARELVKQGHGDIVIIEKEPELGVHASGRNSGVLHAGIYYSPDSLKAKSCLNGNFLMREYC
KEKGLPLLESGKVIVTRTAAELPVLDELHRRATANGAKVEMIDEKQLAAIEPNARTVERALFSHYTAVVDPKAVLKSLKK
DLERTGRVRLHLGCKMLGLKGSSTVVTNKGDISFERFINAAGAYCNKVAGFFGVGSKYRLIPFKGVYRLLKKDAPFTVNS
NIYPVPDIRNPFLGIHFTRSVHGDVYLGPTAIPAFGRENYGILSGIDAEAFSIAWQDLVLFLLNRPFRNVALSEPLKYFP
SYFFRDAARLVKELAPSDVVHASKVGIRPQLVDWEKKELVMDFLVVADGSSLHVLNPISPAFTSSMDLAQGMVAEHFSS

Sequences:

>Translated_399_residues
MSFDNAGILIVGGGIIGLTIARELVKQGHGDIVIIEKEPELGVHASGRNSGVLHAGIYYSPDSLKAKSCLNGNFLMREYC
KEKGLPLLESGKVIVTRTAAELPVLDELHRRATANGAKVEMIDEKQLAAIEPNARTVERALFSHYTAVVDPKAVLKSLKK
DLERTGRVRLHLGCKMLGLKGSSTVVTNKGDISFERFINAAGAYCNKVAGFFGVGSKYRLIPFKGVYRLLKKDAPFTVNS
NIYPVPDIRNPFLGIHFTRSVHGDVYLGPTAIPAFGRENYGILSGIDAEAFSIAWQDLVLFLLNRPFRNVALSEPLKYFP
SYFFRDAARLVKELAPSDVVHASKVGIRPQLVDWEKKELVMDFLVVADGSSLHVLNPISPAFTSSMDLAQGMVAEHFSS
>Mature_398_residues
SFDNAGILIVGGGIIGLTIARELVKQGHGDIVIIEKEPELGVHASGRNSGVLHAGIYYSPDSLKAKSCLNGNFLMREYCK
EKGLPLLESGKVIVTRTAAELPVLDELHRRATANGAKVEMIDEKQLAAIEPNARTVERALFSHYTAVVDPKAVLKSLKKD
LERTGRVRLHLGCKMLGLKGSSTVVTNKGDISFERFINAAGAYCNKVAGFFGVGSKYRLIPFKGVYRLLKKDAPFTVNSN
IYPVPDIRNPFLGIHFTRSVHGDVYLGPTAIPAFGRENYGILSGIDAEAFSIAWQDLVLFLLNRPFRNVALSEPLKYFPS
YFFRDAARLVKELAPSDVVHASKVGIRPQLVDWEKKELVMDFLVVADGSSLHVLNPISPAFTSSMDLAQGMVAEHFSS

Specific function: Oxidizes L-2-hydroxyglutarate, probably to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate [H]

COG id: COG0579

COG function: function code R; Predicted dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L2HGDH family [H]

Homologues:

Organism=Homo sapiens, GI13376331, Length=394, Percent_Identity=32.741116751269, Blast_Score=194, Evalue=1e-49,
Organism=Escherichia coli, GI87082148, Length=367, Percent_Identity=29.4277929155313, Blast_Score=160, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17565764, Length=410, Percent_Identity=36.3414634146341, Blast_Score=221, Evalue=5e-58,
Organism=Drosophila melanogaster, GI24585081, Length=405, Percent_Identity=32.0987654320988, Blast_Score=169, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015657
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.1.3.15 [H]

Molecular weight: Translated: 43715; Mature: 43584

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFDNAGILIVGGGIIGLTIARELVKQGHGDIVIIEKEPELGVHASGRNSGVLHAGIYYS
CCCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEECCCCCCEEEEEEEEC
PDSLKAKSCLNGNFLMREYCKEKGLPLLESGKVIVTRTAAELPVLDELHRRATANGAKVE
CCCCCHHHHCCCCHHHHHHHHHCCCCEECCCCEEEEECCCCCCHHHHHHHHHCCCCCEEE
MIDEKQLAAIEPNARTVERALFSHYTAVVDPKAVLKSLKKDLERTGRVRLHLGCKMLGLK
EECCCCCEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECEEEEECC
GSSTVVTNKGDISFERFINAAGAYCNKVAGFFGVGSKYRLIPFKGVYRLLKKDAPFTVNS
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCEEECC
NIYPVPDIRNPFLGIHFTRSVHGDVYLGPTAIPAFGRENYGILSGIDAEAFSIAWQDLVL
CEEECCCCCCCCEEEEEEEECCCCEEECCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHH
FLLNRPFRNVALSEPLKYFPSYFFRDAARLVKELAPSDVVHASKVGIRPQLVDWEKKELV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCHHHHHH
MDFLVVADGSSLHVLNPISPAFTSSMDLAQGMVAEHFSS
HHHHEEECCCCEEEECCCCHHHHCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SFDNAGILIVGGGIIGLTIARELVKQGHGDIVIIEKEPELGVHASGRNSGVLHAGIYYS
CCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEECCCCCCEEEEEEEEC
PDSLKAKSCLNGNFLMREYCKEKGLPLLESGKVIVTRTAAELPVLDELHRRATANGAKVE
CCCCCHHHHCCCCHHHHHHHHHCCCCEECCCCEEEEECCCCCCHHHHHHHHHCCCCCEEE
MIDEKQLAAIEPNARTVERALFSHYTAVVDPKAVLKSLKKDLERTGRVRLHLGCKMLGLK
EECCCCCEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECEEEEECC
GSSTVVTNKGDISFERFINAAGAYCNKVAGFFGVGSKYRLIPFKGVYRLLKKDAPFTVNS
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCEEECC
NIYPVPDIRNPFLGIHFTRSVHGDVYLGPTAIPAFGRENYGILSGIDAEAFSIAWQDLVL
CEEECCCCCCCCEEEEEEEECCCCEEECCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHH
FLLNRPFRNVALSEPLKYFPSYFFRDAARLVKELAPSDVVHASKVGIRPQLVDWEKKELV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCHHHHHH
MDFLVVADGSSLHVLNPISPAFTSSMDLAQGMVAEHFSS
HHHHEEECCCCEEEECCCCHHHHCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8297211; 7984428 [H]