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The map label for this gene is pheS

Identifier: 197118376

GI number: 197118376

Start: 2298626

End: 2299642

Strand: Direct

Name: pheS

Synonym: Gbem_1994

Alternate gene names: 197118376

Gene position: 2298626-2299642 (Clockwise)

Preceding gene: 197118375

Following gene: 197118377

Centisome position: 49.81

GC content: 62.93

Gene sequence:

>1017_bases
ATGAAGGATAAACTGGAAGCACTATTAGATCAGGCCCTCTCCGAGCTGGCGCAAGCCTCCACCGAGGAGGGCGTGCAGGA
GCTGCGGGTCAAGTACCTGGGGAAAAAGGGTGAGCTGACCTCCGTGATGAAGGGGTTGGGCGCGCTCACCCCGGAGGAAC
GCCCCGTCATCGGCCAGGTGGTGAACACGGTCAAAAGCAAGCTCGAAGAGGCGTTCGAGGTCCGCGGCGGCGAGATCCGC
GAGGTCGTAAAGAGCGCCCGGCTTTCCGCCGAGAGGATCGACGTGACCCTTCCCGGCCGCCGCCGTCCGCTGGGCTCCAA
GCATCCCATCACGCTCGTCACCGAGGAGATCACCTCCATCTTCGGCGCGCTCGGCTTCGCCGTAGCCGAAGGGCCCGAGA
TCGAGCTCGACTTCTACAACTTCGAGGCCCTCAACCTGCCGAAGGATCATCCCGCCCGCGACATGCAGGACACCTTCTAC
TTCGGCGAGAGCGTTCTTTTGAGGACCCATACCTCTCCGGTGCAGATCCGCACCATGCTGAAGCAGCCGCCGCCGGTCCG
CATCATCGCCCCCGGCACCGTGTACCGCTGCGATTCCGACGCCACCCACTCCCCGATGTTCCATCAGGTCGAGGGGCTCA
TGGTCGACAAGGGAATCACCTTCGGCGATCTCAAAGGGATCCTGACCCTCTTCATCAGCCAGCTCTTCGGCTCCGACATC
GGCGTTAGACTGCGCCCGTCGTTCTTCCCGTTCACCGAGCCGTCGGCCGAGGTCGACATCGCCTGCGTCATCTGCCGCGG
CAAAGGGTGCCGGGTCTGCAAGGAGACCGGCTGGCTAGAGATCCTGGGCGCCGGCATGGTCGACCCCGAGGTGTACCGCC
ACGTAGGCTACGACTCCGAGCTTTACACCGGCTTCGCCTTCGGGATGGGTATCGAGAGGATCGCCATGCTGAAGTACGGC
ATCGCCGACATGCGGCTCCTGTTCGAAAACGACCTCAGGTTCCTTAAACAGTTCTAA

Upstream 100 bases:

>100_bases
CGGCAGCCAAAGCAAAATTTTAATACATACCGCGGGCATAAAAAAAGAAATGGGATAGCGATGTCTCATTTCTTTTTTTT
TCCGTAATAGGGCATTGCAC

Downstream 100 bases:

>100_bases
AAACAAAGTCAAAAGCACGCACGCGGAAAAGGCAAAAGCTGACGGATAGAAACAGATCAAGCTTTAAGGCTTGGTTCAAG
CTTTTAACCCAAGCCTTCTG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKDKLEALLDQALSELAQASTEEGVQELRVKYLGKKGELTSVMKGLGALTPEERPVIGQVVNTVKSKLEEAFEVRGGEIR
EVVKSARLSAERIDVTLPGRRRPLGSKHPITLVTEEITSIFGALGFAVAEGPEIELDFYNFEALNLPKDHPARDMQDTFY
FGESVLLRTHTSPVQIRTMLKQPPPVRIIAPGTVYRCDSDATHSPMFHQVEGLMVDKGITFGDLKGILTLFISQLFGSDI
GVRLRPSFFPFTEPSAEVDIACVICRGKGCRVCKETGWLEILGAGMVDPEVYRHVGYDSELYTGFAFGMGIERIAMLKYG
IADMRLLFENDLRFLKQF

Sequences:

>Translated_338_residues
MKDKLEALLDQALSELAQASTEEGVQELRVKYLGKKGELTSVMKGLGALTPEERPVIGQVVNTVKSKLEEAFEVRGGEIR
EVVKSARLSAERIDVTLPGRRRPLGSKHPITLVTEEITSIFGALGFAVAEGPEIELDFYNFEALNLPKDHPARDMQDTFY
FGESVLLRTHTSPVQIRTMLKQPPPVRIIAPGTVYRCDSDATHSPMFHQVEGLMVDKGITFGDLKGILTLFISQLFGSDI
GVRLRPSFFPFTEPSAEVDIACVICRGKGCRVCKETGWLEILGAGMVDPEVYRHVGYDSELYTGFAFGMGIERIAMLKYG
IADMRLLFENDLRFLKQF
>Mature_338_residues
MKDKLEALLDQALSELAQASTEEGVQELRVKYLGKKGELTSVMKGLGALTPEERPVIGQVVNTVKSKLEEAFEVRGGEIR
EVVKSARLSAERIDVTLPGRRRPLGSKHPITLVTEEITSIFGALGFAVAEGPEIELDFYNFEALNLPKDHPARDMQDTFY
FGESVLLRTHTSPVQIRTMLKQPPPVRIIAPGTVYRCDSDATHSPMFHQVEGLMVDKGITFGDLKGILTLFISQLFGSDI
GVRLRPSFFPFTEPSAEVDIACVICRGKGCRVCKETGWLEILGAGMVDPEVYRHVGYDSELYTGFAFGMGIERIAMLKYG
IADMRLLFENDLRFLKQF

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=289, Percent_Identity=30.4498269896194, Blast_Score=124, Evalue=9e-29,
Organism=Homo sapiens, GI4758340, Length=271, Percent_Identity=31.3653136531365, Blast_Score=118, Evalue=1e-26,
Organism=Escherichia coli, GI1788007, Length=334, Percent_Identity=54.4910179640719, Blast_Score=372, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17508957, Length=269, Percent_Identity=31.2267657992565, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI32563657, Length=269, Percent_Identity=31.2267657992565, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI32566635, Length=256, Percent_Identity=32.03125, Blast_Score=115, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6321087, Length=269, Percent_Identity=31.2267657992565, Blast_Score=133, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6325304, Length=328, Percent_Identity=27.4390243902439, Blast_Score=110, Evalue=4e-25,
Organism=Drosophila melanogaster, GI18858079, Length=270, Percent_Identity=30.3703703703704, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17137424, Length=299, Percent_Identity=27.4247491638796, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_GEOBB (B5EBY3)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002138803.1
- ProteinModelPortal:   B5EBY3
- SMR:   B5EBY3
- GeneID:   6781986
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_1994
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 37593; Mature: 37593

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDKLEALLDQALSELAQASTEEGVQELRVKYLGKKGELTSVMKGLGALTPEERPVIGQV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHH
VNTVKSKLEEAFEVRGGEIREVVKSARLSAERIDVTLPGRRRPLGSKHPITLVTEEITSI
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHEEEECCCCCCCCCCCCCEEEHHHHHHHH
FGALGFAVAEGPEIELDFYNFEALNLPKDHPARDMQDTFYFGESVLLRTHTSPVQIRTML
HHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCHHHHHHHCCEEEEEECCCHHHHHHHH
KQPPPVRIIAPGTVYRCDSDATHSPMFHQVEGLMVDKGITFGDLKGILTLFISQLFGSDI
CCCCCEEEECCCEEEECCCCCCCCCHHHHHHCEEEECCCCHHHHHHHHHHHHHHHHCCCC
GVRLRPSFFPFTEPSAEVDIACVICRGKGCRVCKETGWLEILGAGMVDPEVYRHVGYDSE
CEEECCCCCCCCCCCCCEEEEEEEECCCCCCHHHHCCCEEEECCCCCCHHHHHHCCCCCH
LYTGFAFGMGIERIAMLKYGIADMRLLFENDLRFLKQF
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKDKLEALLDQALSELAQASTEEGVQELRVKYLGKKGELTSVMKGLGALTPEERPVIGQV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHH
VNTVKSKLEEAFEVRGGEIREVVKSARLSAERIDVTLPGRRRPLGSKHPITLVTEEITSI
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHEEEECCCCCCCCCCCCCEEEHHHHHHHH
FGALGFAVAEGPEIELDFYNFEALNLPKDHPARDMQDTFYFGESVLLRTHTSPVQIRTML
HHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCHHHHHHHCCEEEEEECCCHHHHHHHH
KQPPPVRIIAPGTVYRCDSDATHSPMFHQVEGLMVDKGITFGDLKGILTLFISQLFGSDI
CCCCCEEEECCCEEEECCCCCCCCCHHHHHHCEEEECCCCHHHHHHHHHHHHHHHHCCCC
GVRLRPSFFPFTEPSAEVDIACVICRGKGCRVCKETGWLEILGAGMVDPEVYRHVGYDSE
CEEECCCCCCCCCCCCCEEEEEEEECCCCCCHHHHCCCEEEECCCCCCHHHHHHCCCCCH
LYTGFAFGMGIERIAMLKYGIADMRLLFENDLRFLKQF
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA