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The map label for this gene is trpB2 [H]

Identifier: 197118341

GI number: 197118341

Start: 2262203

End: 2263558

Strand: Direct

Name: trpB2 [H]

Synonym: Gbem_1958

Alternate gene names: 197118341

Gene position: 2262203-2263558 (Clockwise)

Preceding gene: 197118340

Following gene: 197118342

Centisome position: 49.02

GC content: 63.72

Gene sequence:

>1356_bases
ATGAATACCAAGTTTATGCTCGATGAATCCCGCATCCCTAAACAGTGGTACAACATCATCCCCGACATGCCTGGGCCGCT
GCATCCTGTCGTAAACCCGCAGACCCTGAAGCCGGTGACTCCGGAGGACATGAGCGTCATCTTCCCCATGTCGCTGATCG
AGCAGGAGATGTCGACCGAGCGTTGGATCGATATTCCCGAAGACGTGCGCGAGAAATACCGGCTCTGGCGGCCGGCCCCG
CTTTTCAGGGCCAGAAGGCTGGAGCAGGCGCTGCAGACCCCGGCGAAGATCTATTACAAGTACGAAGGGGTCTCGCCCGC
CGGCTCTCATAAGCCGAACTCCGCCATCCCGCAGGCCTACTACAACAAGCAGGCGGGTATCCGCAGGCTCGCCACCGAGA
CCGGCGCGGGGCAATGGGGTAGCTCGCTGGCTCTGGCCTGCTCCATGTTCGGGCTGGAGTGCACCATCTATATGGTGAAG
GTCTCCTGCACCCAGAAGCCGTACCGAAAGAGCATGATGCAGCTTTGGGGCGCGACGGTGATCCCGTCCCCCTCCGAGTT
CACCAACGCGGGGCGCTCGATTCTGGCGCATGACCCGAACAACCTGGGAAGCCTCGGCATCGCCATCTCCGAGGCGGTAG
AGGACGCGGCGACCCACGACGACACGAACTACGCGCTGGGGAGCGTCTTGAACCACGTCTGCCTGCACCAGACCGTGATC
GGGCTGGAGGCGAAGGAGCAGATGGAGCTGGCCGGGGATTACCCGGACATCGTCATCGCCTGCTGCGGCGGCGGCTCCAA
CTTCGCCGGGCTCGCCTTCCCCTTCCTCTCCGACCGCGCCAACGGGAAAAAGGTGCGCTGCCTGGCAGTGGAGCCGGCTT
CCTGCCCGACCCTGACCAAGGGGATTTACGCCTTCGATTACGGCGACACGGCGAAGCTCGCCCCCATCACCAAGATGTAC
ACCCTGGGGCACGACTTCATGCCGCCGGGGATCCATGCCGGCGGGCTGCGTTACCACGGCGAGTCCGCGCTCATCTCCCA
GCTCTACCACGCCGGAGAGATCGAGGCGAAGTCGTACAAGCAGAACGCCTGCTTCGAAGGGGCCCTGCTCTTCGCCAGGA
GCGAGGGGATCGTTCCGGCGCCGGAATCCTCCCATGCGGTGCGCGCCGCCATCGACGAGGCGGTGCTGGCCAAGGAGGAA
GGGAAGGAGAAGACCATCCTCTTCGGGCTTTCCGGCCACGGGCAGCTCGACATGATGGCTTACGACGCCTACCTCTCCGG
CGCGCTCGAGGACTTCGAGTACCCCGAGGAGATGATCCGGCAATCGCTGGAGCGGCTGCCGAAGGTTTCCTTCTAG

Upstream 100 bases:

>100_bases
CCTTTCTCATCGGCGAGTCGCTGATGCGCGAGGACGACTTCGGCGCGAAGCTGCGCGAACTGGTGGGTTAACTGCGACAA
TCCGTCCCAAAGGAGTGGCT

Downstream 100 bases:

>100_bases
GGACGATAGGGGACTGGCTCCGCAAGGTGCCTGTCCCCGTTGCTGCTAAGGACGACGATTGCCCCATGTCAAGGGCGCTG
AAGGTCTGCCTGCTGGAAAG

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNTKFMLDESRIPKQWYNIIPDMPGPLHPVVNPQTLKPVTPEDMSVIFPMSLIEQEMSTERWIDIPEDVREKYRLWRPAP
LFRARRLEQALQTPAKIYYKYEGVSPAGSHKPNSAIPQAYYNKQAGIRRLATETGAGQWGSSLALACSMFGLECTIYMVK
VSCTQKPYRKSMMQLWGATVIPSPSEFTNAGRSILAHDPNNLGSLGIAISEAVEDAATHDDTNYALGSVLNHVCLHQTVI
GLEAKEQMELAGDYPDIVIACCGGGSNFAGLAFPFLSDRANGKKVRCLAVEPASCPTLTKGIYAFDYGDTAKLAPITKMY
TLGHDFMPPGIHAGGLRYHGESALISQLYHAGEIEAKSYKQNACFEGALLFARSEGIVPAPESSHAVRAAIDEAVLAKEE
GKEKTILFGLSGHGQLDMMAYDAYLSGALEDFEYPEEMIRQSLERLPKVSF

Sequences:

>Translated_451_residues
MNTKFMLDESRIPKQWYNIIPDMPGPLHPVVNPQTLKPVTPEDMSVIFPMSLIEQEMSTERWIDIPEDVREKYRLWRPAP
LFRARRLEQALQTPAKIYYKYEGVSPAGSHKPNSAIPQAYYNKQAGIRRLATETGAGQWGSSLALACSMFGLECTIYMVK
VSCTQKPYRKSMMQLWGATVIPSPSEFTNAGRSILAHDPNNLGSLGIAISEAVEDAATHDDTNYALGSVLNHVCLHQTVI
GLEAKEQMELAGDYPDIVIACCGGGSNFAGLAFPFLSDRANGKKVRCLAVEPASCPTLTKGIYAFDYGDTAKLAPITKMY
TLGHDFMPPGIHAGGLRYHGESALISQLYHAGEIEAKSYKQNACFEGALLFARSEGIVPAPESSHAVRAAIDEAVLAKEE
GKEKTILFGLSGHGQLDMMAYDAYLSGALEDFEYPEEMIRQSLERLPKVSF
>Mature_451_residues
MNTKFMLDESRIPKQWYNIIPDMPGPLHPVVNPQTLKPVTPEDMSVIFPMSLIEQEMSTERWIDIPEDVREKYRLWRPAP
LFRARRLEQALQTPAKIYYKYEGVSPAGSHKPNSAIPQAYYNKQAGIRRLATETGAGQWGSSLALACSMFGLECTIYMVK
VSCTQKPYRKSMMQLWGATVIPSPSEFTNAGRSILAHDPNNLGSLGIAISEAVEDAATHDDTNYALGSVLNHVCLHQTVI
GLEAKEQMELAGDYPDIVIACCGGGSNFAGLAFPFLSDRANGKKVRCLAVEPASCPTLTKGIYAFDYGDTAKLAPITKMY
TLGHDFMPPGIHAGGLRYHGESALISQLYHAGEIEAKSYKQNACFEGALLFARSEGIVPAPESSHAVRAAIDEAVLAKEE
GKEKTILFGLSGHGQLDMMAYDAYLSGALEDFEYPEEMIRQSLERLPKVSF

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=360, Percent_Identity=29.4444444444444, Blast_Score=109, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321412, Length=375, Percent_Identity=31.7333333333333, Blast_Score=131, Evalue=3e-31,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 49624; Mature: 49624

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTKFMLDESRIPKQWYNIIPDMPGPLHPVVNPQTLKPVTPEDMSVIFPMSLIEQEMSTE
CCCCEECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHH
RWIDIPEDVREKYRLWRPAPLFRARRLEQALQTPAKIYYKYEGVSPAGSHKPNSAIPQAY
HCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHH
YNKQAGIRRLATETGAGQWGSSLALACSMFGLECTIYMVKVSCTQKPYRKSMMQLWGATV
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEEEEEEEEECCCCHHHHHHHHHHCCCC
IPSPSEFTNAGRSILAHDPNNLGSLGIAISEAVEDAATHDDTNYALGSVLNHVCLHQTVI
CCCCHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
GLEAKEQMELAGDYPDIVIACCGGGSNFAGLAFPFLSDRANGKKVRCLAVEPASCPTLTK
CCCCHHHHHHCCCCCCEEEEEECCCCCCCCEECHHHHCCCCCCEEEEEEECCCCCCHHHC
GIYAFDYGDTAKLAPITKMYTLGHDFMPPGIHAGGLRYHGESALISQLYHAGEIEAKSYK
CEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCHHHHH
QNACFEGALLFARSEGIVPAPESSHAVRAAIDEAVLAKEEGKEKTILFGLSGHGQLDMMA
CCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEHEE
YDAYLSGALEDFEYPEEMIRQSLERLPKVSF
HHHHHHHHHHHHCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNTKFMLDESRIPKQWYNIIPDMPGPLHPVVNPQTLKPVTPEDMSVIFPMSLIEQEMSTE
CCCCEECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHH
RWIDIPEDVREKYRLWRPAPLFRARRLEQALQTPAKIYYKYEGVSPAGSHKPNSAIPQAY
HCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHH
YNKQAGIRRLATETGAGQWGSSLALACSMFGLECTIYMVKVSCTQKPYRKSMMQLWGATV
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEEEEEEEEECCCCHHHHHHHHHHCCCC
IPSPSEFTNAGRSILAHDPNNLGSLGIAISEAVEDAATHDDTNYALGSVLNHVCLHQTVI
CCCCHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
GLEAKEQMELAGDYPDIVIACCGGGSNFAGLAFPFLSDRANGKKVRCLAVEPASCPTLTK
CCCCHHHHHHCCCCCCEEEEEECCCCCCCCEECHHHHCCCCCCEEEEEEECCCCCCHHHC
GIYAFDYGDTAKLAPITKMYTLGHDFMPPGIHAGGLRYHGESALISQLYHAGEIEAKSYK
CEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCHHHHH
QNACFEGALLFARSEGIVPAPESSHAVRAAIDEAVLAKEEGKEKTILFGLSGHGQLDMMA
CCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEHEE
YDAYLSGALEDFEYPEEMIRQSLERLPKVSF
HHHHHHHHHHHHCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]