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The map label for this gene is livM [H]

Identifier: 197118327

GI number: 197118327

Start: 2248094

End: 2248987

Strand: Direct

Name: livM [H]

Synonym: Gbem_1944

Alternate gene names: 197118327

Gene position: 2248094-2248987 (Clockwise)

Preceding gene: 197118326

Following gene: 197118328

Centisome position: 48.71

GC content: 62.42

Gene sequence:

>894_bases
ATGGCAGATTTCCTTAATAGCTTGGTCGGCGCCGTCGACCCGTACATGATGCAGATCCTGGTCAACATCGGCATCGCTAT
CGTGCTGGCGCTGGGGCTCAACGTGATCGTCGGTCTCACCGGGCAGCTGTCGCTGGGGCACGCTGCCTTCATGAGCATTG
GAGCCTTCACCAGCGCCATGGTCACCATCAAGACCGGACTCCCCTTCAGCATGAACCTCCTGGTCACCGGCGTGGTCACC
GGCATCGTGGCTGCCGCCATCGGCTTCCCGATCCTGAGGCTGACCGGCGACTATCTCGCCATCTGCACCCTGGGCTTCGC
CGAGATCGTGAAGGTGTTCTTCCTGAACTTCGAGCCGACCAACAAGGCGCTCGGCCTCACGGTCCCCCCGGCGAAGACCA
TGATCCCCATGCCGATCTACGTATGGGTGGTGGCTATCCTTTCCATCGTGCTGGTCACCTTCGTGCAGTCCTCCCGGTTC
GGGCGGGCGCTGAAGGCGATCCGCGAGGACGAGATCGCAGCCGAGGCGATGGGAATCAACACCGCACGCTACAAGATCCA
GGCCTTCGCGCTCGGCTCCTTCATGGCCGGGGTCGGCGGCGGCCTTTACGCCCACTTCCTGAGCTACATCAACCCCTCGG
ACTTCGGCTTCCTCAAATCGGTCGATATTCTCGCCATGGTGGTATTGGGCGGCCTGGGGAGCGTCCCCGGCACGGTCATC
GGCTCGTCCTTGCTTGCCTCGGCGCCCGAGTTCCTGCGCTTCATGTCCCAGTACCGCATGCTGGTCTACGGCGCGCTGCT
GGTCTTCTTGATGGTGTTCCGCCCCAACGGGCTTCTGGGCGGCGTCAACTTCACGGAACTGCTGCTGCGGGCCATCGGCA
AGAAACGTAAATAA

Upstream 100 bases:

>100_bases
CCTACCGTGATGCGATCGCCTTTACCATTCTCGTGCTCGTTCTCCTTGTAAAGCCGACAGGCCTTTTGGGACAAAAAATT
ACAAAGAAGGTGTAGATTCG

Downstream 100 bases:

>100_bases
TTTCACTGAGCGAGAGCAAAGCATGTCGATACTTAAACTCGAAGACGTCACCATCCGCTTCGGCGGCCTGGTCGCGGTGG
ACAAGGTAAACCTCACGGTA

Product: branched-chain amino acid ABC transporter membrane protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MADFLNSLVGAVDPYMMQILVNIGIAIVLALGLNVIVGLTGQLSLGHAAFMSIGAFTSAMVTIKTGLPFSMNLLVTGVVT
GIVAAAIGFPILRLTGDYLAICTLGFAEIVKVFFLNFEPTNKALGLTVPPAKTMIPMPIYVWVVAILSIVLVTFVQSSRF
GRALKAIREDEIAAEAMGINTARYKIQAFALGSFMAGVGGGLYAHFLSYINPSDFGFLKSVDILAMVVLGGLGSVPGTVI
GSSLLASAPEFLRFMSQYRMLVYGALLVFLMVFRPNGLLGGVNFTELLLRAIGKKRK

Sequences:

>Translated_297_residues
MADFLNSLVGAVDPYMMQILVNIGIAIVLALGLNVIVGLTGQLSLGHAAFMSIGAFTSAMVTIKTGLPFSMNLLVTGVVT
GIVAAAIGFPILRLTGDYLAICTLGFAEIVKVFFLNFEPTNKALGLTVPPAKTMIPMPIYVWVVAILSIVLVTFVQSSRF
GRALKAIREDEIAAEAMGINTARYKIQAFALGSFMAGVGGGLYAHFLSYINPSDFGFLKSVDILAMVVLGGLGSVPGTVI
GSSLLASAPEFLRFMSQYRMLVYGALLVFLMVFRPNGLLGGVNFTELLLRAIGKKRK
>Mature_296_residues
ADFLNSLVGAVDPYMMQILVNIGIAIVLALGLNVIVGLTGQLSLGHAAFMSIGAFTSAMVTIKTGLPFSMNLLVTGVVTG
IVAAAIGFPILRLTGDYLAICTLGFAEIVKVFFLNFEPTNKALGLTVPPAKTMIPMPIYVWVVAILSIVLVTFVQSSRFG
RALKAIREDEIAAEAMGINTARYKIQAFALGSFMAGVGGGLYAHFLSYINPSDFGFLKSVDILAMVVLGGLGSVPGTVIG
SSLLASAPEFLRFMSQYRMLVYGALLVFLMVFRPNGLLGGVNFTELLLRAIGKKRK

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=287, Percent_Identity=35.5400696864112, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1789866, Length=279, Percent_Identity=29.0322580645161, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 31555; Mature: 31424

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADFLNSLVGAVDPYMMQILVNIGIAIVLALGLNVIVGLTGQLSLGHAAFMSIGAFTSAM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
VTIKTGLPFSMNLLVTGVVTGIVAAAIGFPILRLTGDYLAICTLGFAEIVKVFFLNFEPT
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCC
NKALGLTVPPAKTMIPMPIYVWVVAILSIVLVTFVQSSRFGRALKAIREDEIAAEAMGIN
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TARYKIQAFALGSFMAGVGGGLYAHFLSYINPSDFGFLKSVDILAMVVLGGLGSVPGTVI
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHH
GSSLLASAPEFLRFMSQYRMLVYGALLVFLMVFRPNGLLGGVNFTELLLRAIGKKRK
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
ADFLNSLVGAVDPYMMQILVNIGIAIVLALGLNVIVGLTGQLSLGHAAFMSIGAFTSAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
VTIKTGLPFSMNLLVTGVVTGIVAAAIGFPILRLTGDYLAICTLGFAEIVKVFFLNFEPT
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCC
NKALGLTVPPAKTMIPMPIYVWVVAILSIVLVTFVQSSRFGRALKAIREDEIAAEAMGIN
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TARYKIQAFALGSFMAGVGGGLYAHFLSYINPSDFGFLKSVDILAMVVLGGLGSVPGTVI
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHH
GSSLLASAPEFLRFMSQYRMLVYGALLVFLMVFRPNGLLGGVNFTELLLRAIGKKRK
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]