Click here to switch to the map view.

The map label for this gene is nadB [H]

Identifier: 197118257

GI number: 197118257

Start: 2168403

End: 2170004

Strand: Direct

Name: nadB [H]

Synonym: Gbem_1874

Alternate gene names: 197118257

Gene position: 2168403-2170004 (Clockwise)

Preceding gene: 197118252

Following gene: 197118258

Centisome position: 46.98

GC content: 62.98

Gene sequence:

>1602_bases
ATGAAGGTGCAGAGTGATTTTCTTGTCATTGGCAGCGGCATCGCGGGGCTTTCCTTCGCGCTGCAAGCGGCGGCTCACGG
TACTGTCGCCCTGCTTGCCAAACGTGATATAGCGGAGACCGCTACTAACTACGCCCAAGGGGGGATCGCGTCGGTTTCCT
CCAACGAGGACTCCTTCGACGCCCACGTGCAGGACACCCTGGTTGCGGGCGCCGGCATCTGCCACGAAGACGTGGTGCGC
CTGGTGGTCGAGGAAGGACCGCAGGTGATCGCCAACCTCATCGAGTGGGGGGTGAAATTCACCAGGAACACCAGCGGCGA
GGCCTACGACCTCACCCGGGAAGGGGGGCACAGCCAGCGGCGCATCCTGCACGCAGCCGACATCACCGGCCGGGAGATCC
AGCGCGCGCTGGTCGAGGCGGCCCACGCCCACGACAACATCACCATCTACGAAAACCATGCGGCCATCGACCTGATCACC
GAGTCCAAGGTGCTCAAGAAGAAAACGGACCAAAACCGATGCCTGGGCGCCCACGTCCTCGACGTGAAGGCGGGCGTGGT
GAAGACCTTCAGCTCCCGCATCACGCTTTTAGCCTCGGGAGGGGCCGGGAAGGTCTATCTATATACCTGCAACCCGGACA
TAGCCTCCGGCGACGGGGTCGCCATGGCCTACCGCGCCGGCGCCACCATTGCCAACATGGAGTTCATGCAGTTCCACCCG
ACCACGCTCTTCCACCCCAACGCGCGCTCTTTCCTGATCTCGGAGGCGGTACGCGGCGAGGGGGCGATCCTCAAGCGGCG
CGACGGCACCGCCTTCATGGAGCGCTACCATCACCTCAAAGACCTGGCCCCCCGCGACATCGTGGCCCGCGCCATCGACA
ACGAGATGAAGACCCACGGTGACGACTGCGTCTACCTGGACATCAGGCACCGCGGCGCCGAATATATCACTTCCCGCTTC
CCCAACATCTACCAGACCTGCCTCGACTACGGCATCGACATGACCAAGGAGATGATCCCGGTCGTGCCCGCTGCGCATTA
TCTGTGCGGCGGGGTGGCGGTGGACAAGAACGCCGAGACCGACGTGAAGCACCTCTACGCCATCGGCGAGGTTGCCTTCA
CCGGCCTGCACGGCGCCAACCGGCTCGCCAGCAACTCCCTTCTGGAAGCGGCCGTCTACGCCGGGCGCGCCTTCCACCAC
GCCGTCGAAATCCTAAAGGGGGGGGGCTTTCCTTCCTACGCCATCCCCGAGTGGGACAGCGGCACCGCTACGAACTCCGA
CGAAATGGTCGTCGTCTCGCAGAACTGGGACGAGATCAGGCGCTTCATGTCCAACTACGTGGGGATCGTGAGAAGCGACA
AGAGGCTCGCGCGCGCCATGCACCGCATCGAGCTGATCCAGGACGAGATCGAGGAGTACTACTGGAACTTCATCGTCACC
TCGGACCTGATCGAGCTCAGAAACATCGCCACCGTCGCCCAGCTCATCGTCACCTGCGCCCAGATGAGGAAGGAGTCGCG
CGGGCTCAACTACAACATCGATTACCCGAACGTGGACGACGCCAACTGGAAGCGCGACACCTTCGTCAAGAAGCAGTTCT
GA

Upstream 100 bases:

>100_bases
GGGGCGATAACCTGTCAAAAGTCTTGACAAAAATCCCGCGGAGATCAATAATTTGCCGGTTTTTCAATCAAACGGCCGAT
TATAGGCTAGCGGGGGAGAT

Downstream 100 bases:

>100_bases
GGAAGACATGACCATAGACGAGATCAGGCAAGAGATAGACCGGCTGGACAGTGAACTGCTGCGTATTTTCAACGAGCGGG
CCTCTCTTGCCCTGAAGATC

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MKVQSDFLVIGSGIAGLSFALQAAAHGTVALLAKRDIAETATNYAQGGIASVSSNEDSFDAHVQDTLVAGAGICHEDVVR
LVVEEGPQVIANLIEWGVKFTRNTSGEAYDLTREGGHSQRRILHAADITGREIQRALVEAAHAHDNITIYENHAAIDLIT
ESKVLKKKTDQNRCLGAHVLDVKAGVVKTFSSRITLLASGGAGKVYLYTCNPDIASGDGVAMAYRAGATIANMEFMQFHP
TTLFHPNARSFLISEAVRGEGAILKRRDGTAFMERYHHLKDLAPRDIVARAIDNEMKTHGDDCVYLDIRHRGAEYITSRF
PNIYQTCLDYGIDMTKEMIPVVPAAHYLCGGVAVDKNAETDVKHLYAIGEVAFTGLHGANRLASNSLLEAAVYAGRAFHH
AVEILKGGGFPSYAIPEWDSGTATNSDEMVVVSQNWDEIRRFMSNYVGIVRSDKRLARAMHRIELIQDEIEEYYWNFIVT
SDLIELRNIATVAQLIVTCAQMRKESRGLNYNIDYPNVDDANWKRDTFVKKQF

Sequences:

>Translated_533_residues
MKVQSDFLVIGSGIAGLSFALQAAAHGTVALLAKRDIAETATNYAQGGIASVSSNEDSFDAHVQDTLVAGAGICHEDVVR
LVVEEGPQVIANLIEWGVKFTRNTSGEAYDLTREGGHSQRRILHAADITGREIQRALVEAAHAHDNITIYENHAAIDLIT
ESKVLKKKTDQNRCLGAHVLDVKAGVVKTFSSRITLLASGGAGKVYLYTCNPDIASGDGVAMAYRAGATIANMEFMQFHP
TTLFHPNARSFLISEAVRGEGAILKRRDGTAFMERYHHLKDLAPRDIVARAIDNEMKTHGDDCVYLDIRHRGAEYITSRF
PNIYQTCLDYGIDMTKEMIPVVPAAHYLCGGVAVDKNAETDVKHLYAIGEVAFTGLHGANRLASNSLLEAAVYAGRAFHH
AVEILKGGGFPSYAIPEWDSGTATNSDEMVVVSQNWDEIRRFMSNYVGIVRSDKRLARAMHRIELIQDEIEEYYWNFIVT
SDLIELRNIATVAQLIVTCAQMRKESRGLNYNIDYPNVDDANWKRDTFVKKQF
>Mature_533_residues
MKVQSDFLVIGSGIAGLSFALQAAAHGTVALLAKRDIAETATNYAQGGIASVSSNEDSFDAHVQDTLVAGAGICHEDVVR
LVVEEGPQVIANLIEWGVKFTRNTSGEAYDLTREGGHSQRRILHAADITGREIQRALVEAAHAHDNITIYENHAAIDLIT
ESKVLKKKTDQNRCLGAHVLDVKAGVVKTFSSRITLLASGGAGKVYLYTCNPDIASGDGVAMAYRAGATIANMEFMQFHP
TTLFHPNARSFLISEAVRGEGAILKRRDGTAFMERYHHLKDLAPRDIVARAIDNEMKTHGDDCVYLDIRHRGAEYITSRF
PNIYQTCLDYGIDMTKEMIPVVPAAHYLCGGVAVDKNAETDVKHLYAIGEVAFTGLHGANRLASNSLLEAAVYAGRAFHH
AVEILKGGGFPSYAIPEWDSGTATNSDEMVVVSQNWDEIRRFMSNYVGIVRSDKRLARAMHRIELIQDEIEEYYWNFIVT
SDLIELRNIATVAQLIVTCAQMRKESRGLNYNIDYPNVDDANWKRDTFVKKQF

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=538, Percent_Identity=29.368029739777, Blast_Score=208, Evalue=1e-53,
Organism=Escherichia coli, GI1788928, Length=518, Percent_Identity=53.6679536679537, Blast_Score=543, Evalue=1e-156,
Organism=Escherichia coli, GI1790597, Length=534, Percent_Identity=31.8352059925094, Blast_Score=245, Evalue=4e-66,
Organism=Escherichia coli, GI1786942, Length=562, Percent_Identity=31.8505338078292, Blast_Score=221, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17550100, Length=542, Percent_Identity=30.9963099630996, Blast_Score=214, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI17505833, Length=533, Percent_Identity=30.5816135084428, Blast_Score=205, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI71986328, Length=418, Percent_Identity=26.7942583732057, Blast_Score=90, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6322701, Length=541, Percent_Identity=32.1626617375231, Blast_Score=231, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6322416, Length=540, Percent_Identity=29.8148148148148, Blast_Score=213, Evalue=7e-56,
Organism=Drosophila melanogaster, GI17137288, Length=532, Percent_Identity=30.2631578947368, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24655642, Length=532, Percent_Identity=30.2631578947368, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24655647, Length=532, Percent_Identity=30.2631578947368, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24663005, Length=541, Percent_Identity=29.3900184842884, Blast_Score=193, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 58823; Mature: 58823

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVQSDFLVIGSGIAGLSFALQAAAHGTVALLAKRDIAETATNYAQGGIASVSSNEDSFD
CCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCEECCCCCCCCH
AHVQDTLVAGAGICHEDVVRLVVEEGPQVIANLIEWGVKFTRNTSGEAYDLTREGGHSQR
HHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHCCEEECCCCCCEEEECCCCCCHHH
RILHAADITGREIQRALVEAAHAHDNITIYENHAAIDLITESKVLKKKTDQNRCLGAHVL
HEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEHHHHHHHHCCCCCCCCCEEE
DVKAGVVKTFSSRITLLASGGAGKVYLYTCNPDIASGDGVAMAYRAGATIANMEFMQFHP
HHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCCCCCCEEEEEECCCEEECCEEEEECC
TTLFHPNARSFLISEAVRGEGAILKRRDGTAFMERYHHLKDLAPRDIVARAIDNEMKTHG
CEEECCCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
DDCVYLDIRHRGAEYITSRFPNIYQTCLDYGIDMTKEMIPVVPAAHYLCGGVAVDKNAET
CCEEEEEEECCCHHHHHHHCCHHHHHHHHCCCCHHHHHHHHCHHHHHHHCCEEECCCCCH
DVKHLYAIGEVAFTGLHGANRLASNSLLEAAVYAGRAFHHAVEILKGGGFPSYAIPEWDS
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
GTATNSDEMVVVSQNWDEIRRFMSNYVGIVRSDKRLARAMHRIELIQDEIEEYYWNFIVT
CCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SDLIELRNIATVAQLIVTCAQMRKESRGLNYNIDYPNVDDANWKRDTFVKKQF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHCCCCCCC
>Mature Secondary Structure
MKVQSDFLVIGSGIAGLSFALQAAAHGTVALLAKRDIAETATNYAQGGIASVSSNEDSFD
CCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCEECCCCCCCCH
AHVQDTLVAGAGICHEDVVRLVVEEGPQVIANLIEWGVKFTRNTSGEAYDLTREGGHSQR
HHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHCCEEECCCCCCEEEECCCCCCHHH
RILHAADITGREIQRALVEAAHAHDNITIYENHAAIDLITESKVLKKKTDQNRCLGAHVL
HEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEHHHHHHHHCCCCCCCCCEEE
DVKAGVVKTFSSRITLLASGGAGKVYLYTCNPDIASGDGVAMAYRAGATIANMEFMQFHP
HHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCCCCCCEEEEEECCCEEECCEEEEECC
TTLFHPNARSFLISEAVRGEGAILKRRDGTAFMERYHHLKDLAPRDIVARAIDNEMKTHG
CEEECCCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
DDCVYLDIRHRGAEYITSRFPNIYQTCLDYGIDMTKEMIPVVPAAHYLCGGVAVDKNAET
CCEEEEEEECCCHHHHHHHCCHHHHHHHHCCCCHHHHHHHHCHHHHHHHCCEEECCCCCH
DVKHLYAIGEVAFTGLHGANRLASNSLLEAAVYAGRAFHHAVEILKGGGFPSYAIPEWDS
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
GTATNSDEMVVVSQNWDEIRRFMSNYVGIVRSDKRLARAMHRIELIQDEIEEYYWNFIVT
CCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SDLIELRNIATVAQLIVTCAQMRKESRGLNYNIDYPNVDDANWKRDTFVKKQF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]