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The map label for this gene is cpaF-2 [C]

Identifier: 197118217

GI number: 197118217

Start: 2129225

End: 2130538

Strand: Reverse

Name: cpaF-2 [C]

Synonym: Gbem_1833

Alternate gene names: 197118217

Gene position: 2130538-2129225 (Counterclockwise)

Preceding gene: 197118218

Following gene: 197118216

Centisome position: 46.16

GC content: 58.6

Gene sequence:

>1314_bases
ATGAGTTTTTCAAACTACCATTCGGGTCAGGAACCTGCGGCAGACCTCGTCTCGCCTTCCTTTCAGGAATTGAAGAGCCG
TATCCATCGCCTGCTTATCGACAGAATAGATCTATCCAAGCTCGAACTGCTCCGTGCCGGCGAATTGGAGCGGGAGATCG
GCGCGGTCATCGAGAGGCTCATCGTGGAGGAAGGAGTTCCATTGAGTCGACAGGAAAAGGAGAGCCTGGTGCTGGAGGTG
CAGCACGAGACGTTCGGTTTGGGTCCCATCGAACCTTTGCTGAGTTCTCCCGACATCTCCGACATTCTGGTCAACAACTG
TTCCAATACATATGTCGAGCGAAACGGCAAACTGGAAAAGACGAATATAGTGTTTCGCGACAACAACCACCTGCGGCAGA
TCATCGAGCGCATCGTAACCAGGGTCGGGAGAAGAATCGACGAGTCATCCCCTTTCGTCGACGCCCGGCTACCCGACGGC
TCCCGTGTCAACGCCATCATCCCCCCGCTGGCCCTGGACGGCCCGGCCCTCTCCATCCGCCGCTTCGGGCTGGTGCCGCT
CAAAATGAACGACCTCCTGGCGCTGGGCACTTTGGACCTGCGCCTGGCAACGCTGATCGAGGGAGCGGTCAAGACGCGGA
TGAACATCCTGATCTGCGGGGGAACCGGCACGGGCAAAACGACTTTGCTCAACGTGATCTCCGAGTTCATACCGGAAAAC
GAGCGAATTCTAACCATCGAGGACTCGGCCGAGTTGCAGTTGAAGCAGGACCATGTGGTGCGGCTGGAAACTCGCCCGCC
AAACATCGAAGGAGCCGGCCAAGTCACCCAGCGAGACCTGGTGCGAAACGCCCTGCGCATGCGCCCCAACAGGATCATAC
TGGGCGAGGTCCGCGGCGCTGAAGCGCTGGACATGCTGCAGGCGATGAATACAGGGCACGACGGCTCGATATCTACTATC
CACTCCAACACGGTGCGCGATGCCTTGTCCCGGCTTTCCACTATGGTCATGATGGCAGGAATGGAGCTGCCTGACCGTGC
CATCAAGGAGCAGATCGCCTCCGCGATCAACATCATCATACACCTCGTGCGGTTCCCCGACGGGACCCGCAGGATCGTCA
AGTTCTCGGAGATAACCGGCATGGAGGGCGAAACCATAGTCATGCAGGATATCGTCTGCTTCGAGCAGATGGGGGTAGAC
CGGGACGGGCGGGTGCAAGGCAGGTTCAGGGGAACCGGTGTTCGGCCAAGGCTTGCGGAACGCCTGAAGATCCACGGACT
CTCCCTGGCGCCGGAAACATTTGAAGGGGACTAA

Upstream 100 bases:

>100_bases
ATCTAACCAGATCGTCTTTTAGCAAATCTATGGACCAACTGGCCCGGCAGTTGTGCCAGGACAGCACCCGCTGAAGAACC
AGCGCTCTAGGGGGAATGAC

Downstream 100 bases:

>100_bases
AGATGACTGCGACTTTGCTGCTGATCTTCGTTGCGGTTTTTCTGGGCACTTTCCCGCTCTTTTCCCTTTTCACGAGGGCG
AGACACTCCTATCTGCTCAG

Product: Flp pilus assembly ATPase CpaF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MSFSNYHSGQEPAADLVSPSFQELKSRIHRLLIDRIDLSKLELLRAGELEREIGAVIERLIVEEGVPLSRQEKESLVLEV
QHETFGLGPIEPLLSSPDISDILVNNCSNTYVERNGKLEKTNIVFRDNNHLRQIIERIVTRVGRRIDESSPFVDARLPDG
SRVNAIIPPLALDGPALSIRRFGLVPLKMNDLLALGTLDLRLATLIEGAVKTRMNILICGGTGTGKTTLLNVISEFIPEN
ERILTIEDSAELQLKQDHVVRLETRPPNIEGAGQVTQRDLVRNALRMRPNRIILGEVRGAEALDMLQAMNTGHDGSISTI
HSNTVRDALSRLSTMVMMAGMELPDRAIKEQIASAINIIIHLVRFPDGTRRIVKFSEITGMEGETIVMQDIVCFEQMGVD
RDGRVQGRFRGTGVRPRLAERLKIHGLSLAPETFEGD

Sequences:

>Translated_437_residues
MSFSNYHSGQEPAADLVSPSFQELKSRIHRLLIDRIDLSKLELLRAGELEREIGAVIERLIVEEGVPLSRQEKESLVLEV
QHETFGLGPIEPLLSSPDISDILVNNCSNTYVERNGKLEKTNIVFRDNNHLRQIIERIVTRVGRRIDESSPFVDARLPDG
SRVNAIIPPLALDGPALSIRRFGLVPLKMNDLLALGTLDLRLATLIEGAVKTRMNILICGGTGTGKTTLLNVISEFIPEN
ERILTIEDSAELQLKQDHVVRLETRPPNIEGAGQVTQRDLVRNALRMRPNRIILGEVRGAEALDMLQAMNTGHDGSISTI
HSNTVRDALSRLSTMVMMAGMELPDRAIKEQIASAINIIIHLVRFPDGTRRIVKFSEITGMEGETIVMQDIVCFEQMGVD
RDGRVQGRFRGTGVRPRLAERLKIHGLSLAPETFEGD
>Mature_436_residues
SFSNYHSGQEPAADLVSPSFQELKSRIHRLLIDRIDLSKLELLRAGELEREIGAVIERLIVEEGVPLSRQEKESLVLEVQ
HETFGLGPIEPLLSSPDISDILVNNCSNTYVERNGKLEKTNIVFRDNNHLRQIIERIVTRVGRRIDESSPFVDARLPDGS
RVNAIIPPLALDGPALSIRRFGLVPLKMNDLLALGTLDLRLATLIEGAVKTRMNILICGGTGTGKTTLLNVISEFIPENE
RILTIEDSAELQLKQDHVVRLETRPPNIEGAGQVTQRDLVRNALRMRPNRIILGEVRGAEALDMLQAMNTGHDGSISTIH
SNTVRDALSRLSTMVMMAGMELPDRAIKEQIASAINIIIHLVRFPDGTRRIVKFSEITGMEGETIVMQDIVCFEQMGVDR
DGRVQGRFRGTGVRPRLAERLKIHGLSLAPETFEGD

Specific function: Unknown

COG id: COG4962

COG function: function code U; Flp pilus assembly protein, ATPase CpaF

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022399
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 48609; Mature: 48478

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSNYHSGQEPAADLVSPSFQELKSRIHRLLIDRIDLSKLELLRAGELEREIGAVIERL
CCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHH
IVEEGVPLSRQEKESLVLEVQHETFGLGPIEPLLSSPDISDILVNNCSNTYVERNGKLEK
HHHCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHHHCCCCCHHHCCCCEEE
TNIVFRDNNHLRQIIERIVTRVGRRIDESSPFVDARLPDGSRVNAIIPPLALDGPALSIR
EEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEECCCCCCCCCCHHHH
RFGLVPLKMNDLLALGTLDLRLATLIEGAVKTRMNILICGGTGTGKTTLLNVISEFIPEN
HCCEEEEECCCEEEECHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCC
ERILTIEDSAELQLKQDHVVRLETRPPNIEGAGQVTQRDLVRNALRMRPNRIILGEVRGA
CEEEEEECCCCEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCH
EALDMLQAMNTGHDGSISTIHSNTVRDALSRLSTMVMMAGMELPDRAIKEQIASAINIII
HHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
HLVRFPDGTRRIVKFSEITGMEGETIVMQDIVCFEQMGVDRDGRVQGRFRGTGVRPRLAE
HHHHCCCCHHHHEEEHHHCCCCCCEEHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHH
RLKIHGLSLAPETFEGD
HHHHCCCEECCCCCCCC
>Mature Secondary Structure 
SFSNYHSGQEPAADLVSPSFQELKSRIHRLLIDRIDLSKLELLRAGELEREIGAVIERL
CCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHH
IVEEGVPLSRQEKESLVLEVQHETFGLGPIEPLLSSPDISDILVNNCSNTYVERNGKLEK
HHHCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHHHCCCCCHHHCCCCEEE
TNIVFRDNNHLRQIIERIVTRVGRRIDESSPFVDARLPDGSRVNAIIPPLALDGPALSIR
EEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEECCCCCCCCCCHHHH
RFGLVPLKMNDLLALGTLDLRLATLIEGAVKTRMNILICGGTGTGKTTLLNVISEFIPEN
HCCEEEEECCCEEEECHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCC
ERILTIEDSAELQLKQDHVVRLETRPPNIEGAGQVTQRDLVRNALRMRPNRIILGEVRGA
CEEEEEECCCCEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCH
EALDMLQAMNTGHDGSISTIHSNTVRDALSRLSTMVMMAGMELPDRAIKEQIASAINIII
HHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
HLVRFPDGTRRIVKFSEITGMEGETIVMQDIVCFEQMGVDRDGRVQGRFRGTGVRPRLAE
HHHHCCCCHHHHEEEHHHCCCCCCEEHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHH
RLKIHGLSLAPETFEGD
HHHHCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036; 9634230 [H]