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The map label for this gene is treZ [H]

Identifier: 197118211

GI number: 197118211

Start: 2122707

End: 2124971

Strand: Direct

Name: treZ [H]

Synonym: Gbem_1827

Alternate gene names: 197118211

Gene position: 2122707-2124971 (Clockwise)

Preceding gene: 197118209

Following gene: 308535266

Centisome position: 45.99

GC content: 61.02

Gene sequence:

>2265_bases
ATGGGAATACACGTCAGCATCCACTATGACAATTCGGGTGGCTTCTCTGATCCTTACCTGTGGGTCTGGTATGCTGGGGC
GGCTGCCGCGGATGACTTCAAGGCCACCGGAACGGACAGCTTTGGCTCTGTGTTCGATGTCGACGTCAAGCGATCAGACT
TTTCCTTCAAATTCAAAAACGGCCCCGGCACCACCGGCGCATGGGAAGGCCCTGGCCTGGACCGGCGCTTCAGGCCACTC
AAGGGGTTGCCCGAGGCCCAGGTGATGACGGAGATCTGGTGCCGGGGGGACAAAGCGTTTGTCTATCCCACCTTGCCGGC
GGTTCCGGAAGCCGACTCGGCAGCAAATTTCCTCAAAACCCTGACCCCCGCCAAAGGAATCTACCTGCCCGATTCAGGCT
CCCTGTCGGGATTGGGCGCCACTACCCTTGCCGGCGGCGGCGTGCTCTTCGGTCTCTACCACCCGAACGCCGGCCGCGTC
TTCGTGTTCGGCAGCTTCAACGACTGGCAAAGACCCGGCCACGACACGCCTCTTCCCGAAAAGTTCTGCGAGCTAAAACT
GTACCGGGGGTACTTCGGCATCCCCAACACCTGGCTCTTGGTACTGCCGCAGGCAAAACCCGGCGACGAGTACAAGTTCT
GCGTGCAGGGGGGCGTGTCAAGCGACGACAAGGGGCGCTTCCAGCAGTACTTCACCGACCCCTATGCCAGAGAGCTTGGC
CCCGACTACGGCATGAACAACAGCAAGATAGTCGACGCCGGGACCTTTGCCTGGACCGATCAGAACTGGCACACGCCCAA
CCGCTCAAAGCTCATCCTGTACGAGATGAGCGTGTACGGCTTCACCGAGGGGGACAACGGGATCGTTAATCCCGGGAAGT
TCGCGGGGATTACCGAGCGGATCGACGCAGGCTACTTCAACGATCTCGGCGTCACCGCATTGGCCCTGATGCCGCTTGCC
GAGTACCCCGGTCCGCAGGGCTCCCGGGTTCTCGGCTACAGCACGTCCCTTTTCTGCGCAGTTGAGCGCGATTTCGGCAG
CTGCGACGACCTGCGGCAGCTGGTCGATAACGCCCACGGCAAGGGGATTGCCGTCATCCTCGACGAGGTGTTCAACCACA
CCAGCAACGACTTCAACCCCCTGTACAAAATGATCCTGGAGCACCCGGACGAGGAGTTCAACCCGGAGGAGGGAGGGCTT
TACTTCAGCGGCAAGACGCGGTGGGGCAACAGGGTCGCGACCGAAAAAGAGGACGTGCAGAACCTCCTGATCGACGCCTG
CAAGCTGATGCTGGTCGAATACCACGTGGACGGATTCCGCTTCGATGCGACCCATACCGACTGGATGGACCATGGCTTCG
TGCTGCGCCTGGCACGGGAGCTTACCTCCTTCCGCCCGTCCGTCGTTCTCATCGCCGAGAACCTCCCGAACCAGCAGGAC
CTGAACCGCTCGGGGTACGACGGCTTCTCCCAGTGGTCCGACCCGTTCCACGACAAGATGAAGGCGCTTTTGCGCGAGGG
GGTTTTCGACAACAGCAACTTCTACAACGCCGACAAACTCGCCGACATCTTTTACTTCAGCAAGTCCCTCTACGCCGCGC
ACACCAACAACGCGGTCAACTACGTCGAAAGCCATGACGAAAGCAGCGTGAGTTACGAGGTCAAAACCAACCCCGCCCTG
GACCAGCCCGCAACCAAAGAGCGCAAGGCGAGGCTGGGATTGTTTTGCACCATGACGGCGCTGGGGATACCGATGCTCTA
CATGGGGGGCGAATTCAATGTCGAGCGGGACAGGAACATCGTGAGCTTCGACTGGCCCAGCGATGGGCCTGGCAGCAACG
GGTTCTTCCGGTGGGCAAGCAGGCTGATCCGGCTGCGCCGGCGCTATCCCGCGCTGCAGATCGAGGGGTACAGCCCAGCC
GAGACGGGGCAGTTCGCCTGGATACTGGGACCTTGGATGGATGGGCGCCACGGCGGGGGGAGCTTGGTTCTCGGGTGGCG
CCTATGGCCGACCCAATTTGCACACGACGCCATGGTGGTGCTGCTCAACTTCGAGAACCGCACGGTGAGCGTGGATCTGG
AGTTGGGAGCGCGGGGTACCTGGCTCAAGCTAGCCGATGCCGACAACGCCAACGATATTGCGCCTGAAGGGAGTAACTCG
GTAAGTGACGCCGCCGCGCTCAAGAGTTCCGACGGGCGCTTCGGCAGTTTCGATCTCCCAAGCTCAAGCTGCTTTCTTTA
CAAATGGGAGAGCGGTCAGGCTTAG

Upstream 100 bases:

>100_bases
GAGTTGACTTGCCGACCTGCTGACATATAGTTCTAACGAAACTTCAGTATCTTACGTGGGAGGATGAGCAAAGGAGAGTT
CAAACGCGAAAGGAGGATGT

Downstream 100 bases:

>100_bases
CAGCGTCCGTGGCGTCCTAGGCGGGTCTTGCTGCTGGTGGGGAGTGAGCACATAAAATCTATAAATCTTATAACATGGGT
GCTATAATGGTCATGGTTTT

Product: alpha amylase

Products: NA

Alternate protein names: MTHase; 4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase; Maltooligosyl trehalose trehalohydrolase [H]

Number of amino acids: Translated: 754; Mature: 753

Protein sequence:

>754_residues
MGIHVSIHYDNSGGFSDPYLWVWYAGAAAADDFKATGTDSFGSVFDVDVKRSDFSFKFKNGPGTTGAWEGPGLDRRFRPL
KGLPEAQVMTEIWCRGDKAFVYPTLPAVPEADSAANFLKTLTPAKGIYLPDSGSLSGLGATTLAGGGVLFGLYHPNAGRV
FVFGSFNDWQRPGHDTPLPEKFCELKLYRGYFGIPNTWLLVLPQAKPGDEYKFCVQGGVSSDDKGRFQQYFTDPYARELG
PDYGMNNSKIVDAGTFAWTDQNWHTPNRSKLILYEMSVYGFTEGDNGIVNPGKFAGITERIDAGYFNDLGVTALALMPLA
EYPGPQGSRVLGYSTSLFCAVERDFGSCDDLRQLVDNAHGKGIAVILDEVFNHTSNDFNPLYKMILEHPDEEFNPEEGGL
YFSGKTRWGNRVATEKEDVQNLLIDACKLMLVEYHVDGFRFDATHTDWMDHGFVLRLARELTSFRPSVVLIAENLPNQQD
LNRSGYDGFSQWSDPFHDKMKALLREGVFDNSNFYNADKLADIFYFSKSLYAAHTNNAVNYVESHDESSVSYEVKTNPAL
DQPATKERKARLGLFCTMTALGIPMLYMGGEFNVERDRNIVSFDWPSDGPGSNGFFRWASRLIRLRRRYPALQIEGYSPA
ETGQFAWILGPWMDGRHGGGSLVLGWRLWPTQFAHDAMVVLLNFENRTVSVDLELGARGTWLKLADADNANDIAPEGSNS
VSDAAALKSSDGRFGSFDLPSSSCFLYKWESGQA

Sequences:

>Translated_754_residues
MGIHVSIHYDNSGGFSDPYLWVWYAGAAAADDFKATGTDSFGSVFDVDVKRSDFSFKFKNGPGTTGAWEGPGLDRRFRPL
KGLPEAQVMTEIWCRGDKAFVYPTLPAVPEADSAANFLKTLTPAKGIYLPDSGSLSGLGATTLAGGGVLFGLYHPNAGRV
FVFGSFNDWQRPGHDTPLPEKFCELKLYRGYFGIPNTWLLVLPQAKPGDEYKFCVQGGVSSDDKGRFQQYFTDPYARELG
PDYGMNNSKIVDAGTFAWTDQNWHTPNRSKLILYEMSVYGFTEGDNGIVNPGKFAGITERIDAGYFNDLGVTALALMPLA
EYPGPQGSRVLGYSTSLFCAVERDFGSCDDLRQLVDNAHGKGIAVILDEVFNHTSNDFNPLYKMILEHPDEEFNPEEGGL
YFSGKTRWGNRVATEKEDVQNLLIDACKLMLVEYHVDGFRFDATHTDWMDHGFVLRLARELTSFRPSVVLIAENLPNQQD
LNRSGYDGFSQWSDPFHDKMKALLREGVFDNSNFYNADKLADIFYFSKSLYAAHTNNAVNYVESHDESSVSYEVKTNPAL
DQPATKERKARLGLFCTMTALGIPMLYMGGEFNVERDRNIVSFDWPSDGPGSNGFFRWASRLIRLRRRYPALQIEGYSPA
ETGQFAWILGPWMDGRHGGGSLVLGWRLWPTQFAHDAMVVLLNFENRTVSVDLELGARGTWLKLADADNANDIAPEGSNS
VSDAAALKSSDGRFGSFDLPSSSCFLYKWESGQA
>Mature_753_residues
GIHVSIHYDNSGGFSDPYLWVWYAGAAAADDFKATGTDSFGSVFDVDVKRSDFSFKFKNGPGTTGAWEGPGLDRRFRPLK
GLPEAQVMTEIWCRGDKAFVYPTLPAVPEADSAANFLKTLTPAKGIYLPDSGSLSGLGATTLAGGGVLFGLYHPNAGRVF
VFGSFNDWQRPGHDTPLPEKFCELKLYRGYFGIPNTWLLVLPQAKPGDEYKFCVQGGVSSDDKGRFQQYFTDPYARELGP
DYGMNNSKIVDAGTFAWTDQNWHTPNRSKLILYEMSVYGFTEGDNGIVNPGKFAGITERIDAGYFNDLGVTALALMPLAE
YPGPQGSRVLGYSTSLFCAVERDFGSCDDLRQLVDNAHGKGIAVILDEVFNHTSNDFNPLYKMILEHPDEEFNPEEGGLY
FSGKTRWGNRVATEKEDVQNLLIDACKLMLVEYHVDGFRFDATHTDWMDHGFVLRLARELTSFRPSVVLIAENLPNQQDL
NRSGYDGFSQWSDPFHDKMKALLREGVFDNSNFYNADKLADIFYFSKSLYAAHTNNAVNYVESHDESSVSYEVKTNPALD
QPATKERKARLGLFCTMTALGIPMLYMGGEFNVERDRNIVSFDWPSDGPGSNGFFRWASRLIRLRRRYPALQIEGYSPAE
TGQFAWILGPWMDGRHGGGSLVLGWRLWPTQFAHDAMVVLLNFENRTVSVDLELGARGTWLKLADADNANDIAPEGSNSV
SDAAALKSSDGRFGSFDLPSSSCFLYKWESGQA

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=588, Percent_Identity=23.9795918367347, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- InterPro:   IPR015156
- InterPro:   IPR012768 [H]

Pfam domain/function: PF09071 Alpha-amyl_C; PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: =3.2.1.141 [H]

Molecular weight: Translated: 83808; Mature: 83677

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIHVSIHYDNSGGFSDPYLWVWYAGAAAADDFKATGTDSFGSVFDVDVKRSDFSFKFKN
CCEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEECC
GPGTTGAWEGPGLDRRFRPLKGLPEAQVMTEIWCRGDKAFVYPTLPAVPEADSAANFLKT
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHH
LTPAKGIYLPDSGSLSGLGATTLAGGGVLFGLYHPNAGRVFVFGSFNDWQRPGHDTPLPE
CCCCCCEECCCCCCCCCCCHHHCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCH
KFCELKLYRGYFGIPNTWLLVLPQAKPGDEYKFCVQGGVSSDDKGRFQQYFTDPYARELG
HHHHEEEECCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHCCHHHHHCC
PDYGMNNSKIVDAGTFAWTDQNWHTPNRSKLILYEMSVYGFTEGDNGIVNPGKFAGITER
CCCCCCCCEEEECCEEEECCCCCCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCCHHHH
IDAGYFNDLGVTALALMPLAEYPGPQGSRVLGYSTSLFCAVERDFGSCDDLRQLVDNAHG
HCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCEEEEEEECCCCCHHHHHHHHHCCCC
KGIAVILDEVFNHTSNDFNPLYKMILEHPDEEFNPEEGGLYFSGKTRWGNRVATEKEDVQ
CEEEEEEHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHH
NLLIDACKLMLVEYHVDGFRFDATHTDWMDHGFVLRLARELTSFRPSVVLIAENLPNQQD
HHHHHHHHHHHHEEECCCEEECCCCCCHHCCHHHHHHHHHHHHCCCCEEEEECCCCCHHH
LNRSGYDGFSQWSDPFHDKMKALLREGVFDNSNFYNADKLADIFYFSKSLYAAHTNNAVN
CCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCH
YVESHDESSVSYEVKTNPALDQPATKERKARLGLFCTMTALGIPMLYMGGEFNVERDRNI
HHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHEEEHHHHCCCEEEECCEECCCCCCCE
VSFDWPSDGPGSNGFFRWASRLIRLRRRYPALQIEGYSPAETGQFAWILGPWMDGRHGGG
EEEECCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEEECCCCCCCCCC
SLVLGWRLWPTQFAHDAMVVLLNFENRTVSVDLELGARGTWLKLADADNANDIAPEGSNS
CEEEEEEECCHHHCCCEEEEEEEECCCEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCC
VSDAAALKSSDGRFGSFDLPSSSCFLYKWESGQA
CHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure 
GIHVSIHYDNSGGFSDPYLWVWYAGAAAADDFKATGTDSFGSVFDVDVKRSDFSFKFKN
CEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEECC
GPGTTGAWEGPGLDRRFRPLKGLPEAQVMTEIWCRGDKAFVYPTLPAVPEADSAANFLKT
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHH
LTPAKGIYLPDSGSLSGLGATTLAGGGVLFGLYHPNAGRVFVFGSFNDWQRPGHDTPLPE
CCCCCCEECCCCCCCCCCCHHHCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCH
KFCELKLYRGYFGIPNTWLLVLPQAKPGDEYKFCVQGGVSSDDKGRFQQYFTDPYARELG
HHHHEEEECCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHCCHHHHHCC
PDYGMNNSKIVDAGTFAWTDQNWHTPNRSKLILYEMSVYGFTEGDNGIVNPGKFAGITER
CCCCCCCCEEEECCEEEECCCCCCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCCHHHH
IDAGYFNDLGVTALALMPLAEYPGPQGSRVLGYSTSLFCAVERDFGSCDDLRQLVDNAHG
HCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCEEEEEEECCCCCHHHHHHHHHCCCC
KGIAVILDEVFNHTSNDFNPLYKMILEHPDEEFNPEEGGLYFSGKTRWGNRVATEKEDVQ
CEEEEEEHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHH
NLLIDACKLMLVEYHVDGFRFDATHTDWMDHGFVLRLARELTSFRPSVVLIAENLPNQQD
HHHHHHHHHHHHEEECCCEEECCCCCCHHCCHHHHHHHHHHHHCCCCEEEEECCCCCHHH
LNRSGYDGFSQWSDPFHDKMKALLREGVFDNSNFYNADKLADIFYFSKSLYAAHTNNAVN
CCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCH
YVESHDESSVSYEVKTNPALDQPATKERKARLGLFCTMTALGIPMLYMGGEFNVERDRNI
HHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHEEEHHHHCCCEEEECCEECCCCCCCE
VSFDWPSDGPGSNGFFRWASRLIRLRRRYPALQIEGYSPAETGQFAWILGPWMDGRHGGG
EEEECCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEEECCCCCCCCCC
SLVLGWRLWPTQFAHDAMVVLLNFENRTVSVDLELGARGTWLKLADADNANDIAPEGSNS
CEEEEEEECCHHHCCCEEEEEEEECCCEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCC
VSDAAALKSSDGRFGSFDLPSSSCFLYKWESGQA
CHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8899719; 11427726 [H]