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The map label for this gene is amiC [H]

Identifier: 197118137

GI number: 197118137

Start: 2017847

End: 2019106

Strand: Reverse

Name: amiC [H]

Synonym: Gbem_1752

Alternate gene names: 197118137

Gene position: 2019106-2017847 (Counterclockwise)

Preceding gene: 197118138

Following gene: 197118135

Centisome position: 43.75

GC content: 61.43

Gene sequence:

>1260_bases
ATGGACAGAAGGGACTTCGTAAAATACCTGGGACTTTCGCTCCCCTACTACCAATGGCTATCCTGCCGGATGCTCGCGGC
ACAGGGGTCGCTGCTGCTCACCCCCTTTCTCTCGGCGCAGGCCGGCGCCGCCACCGCTCAAGGGTTGGTCCCGGTCACCG
AACTGAAACACTGGTCCACCCCCGACTACACCCGCATCGCCATCACCGCAGGGAGCGAGATCCCCTTCGAGCACCACAAG
CTCCCGAACCGCCTCTACCTGGACCTGCACGGCGCCAAGCTGAACCCGGGGGTCAAGGACCTGAGCATCGGGGACGGCCT
GCTGAAAAGCGTGAGGATCGCTCAGTTTCAGGCGGCGACCGTCCGTGTGGTGCTCGATCTGGACAGCATCAAGGACTACA
AGGTCTTCACCTTCTCCGACCCGTTCCGTATCATCGTCGACGTGAAGGGCGACCGCCGGCAGGAGATCTCCGCGTCCAAG
GAAGTTATCGAAACCGCTCCCCCCGAGCCGAAGCCGACGGAGAATGCGAAGCTGGCTGAGAAGCCTAAGTCCGCCGGCAA
GTTCAAGCCCGGCAGGATCAGGAGGATCGTCATCGATCCGGGCCACGGAGGGCACGACCCCGGCGCCGTCAGCCCCTCGG
GGACCCGCGAAAAGGACATCGTGCTCCAGATCGGCCTCAAACTGGCGCAAAAGGTGCGGGAGGAACTGGGGATCGATGCG
GTGATGACCAGGTCGACCGACGTCTTCATTGAGTTGCAGGAGAGAACGGCCATGGCCAACAAGGTGGGAGCCGACCTCTT
CGTCTCCGTCCATGCCAACGCGTCCCTTAGCCGCGCCGCCAACGGCATCGAGACCTATTACCTCAACCTGGCCAAGACCG
AGAAAGCAGCCCAGCTCGCGGCCAAGGAAAACGGCACCTCGCTGGAAAAGGTAAGCATGCTGCAGGCGGTCCTATTCGAC
CTGATGGCTAACTACAAGCTGAACGACTCCGCGCATCTGGCCGACGAGGTGCAAAAGGCGCTTTTCAAAAAGGCGCAGAC
GGGTTACGCAACAGTGAAAAACCTGGGGGTGAAACAGGGGCCCTTCTACGTGCTGGTCGGGGCCACGATGCCGAGCATCC
TGGTGGAAACAGCATTCCTCAGCAACGACCGCGACGAGCAAAAGCTCAAGGATCCCCAATACCAAGAGCTGACAGCCGAC
GGAATCCTCTCCGGCATCAAGGGGTACATCGCCTCGCTCAACAAGGTCCACGCCGGGTAA

Upstream 100 bases:

>100_bases
CGACCAGATCAGGGAGAGGCTCAAGGAAGTAGAAGTGGCGCTTCTTACACCCCTGGAAGCGCTGAACCTCGTGGACGAAC
TAAAGAGGATGATCTAGAAG

Downstream 100 bases:

>100_bases
AGCATCGCCAGGATAACCTGGGTGCGCAGCAACACTGCAGACGCAAAAAGGCCGGCATCAAGCCGGCCTTTTTGCGTTTC
AGGAAACTGGAGCTGTCGTT

Product: N-acetylmuramoyl-L-alanine amidase, family 3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MDRRDFVKYLGLSLPYYQWLSCRMLAAQGSLLLTPFLSAQAGAATAQGLVPVTELKHWSTPDYTRIAITAGSEIPFEHHK
LPNRLYLDLHGAKLNPGVKDLSIGDGLLKSVRIAQFQAATVRVVLDLDSIKDYKVFTFSDPFRIIVDVKGDRRQEISASK
EVIETAPPEPKPTENAKLAEKPKSAGKFKPGRIRRIVIDPGHGGHDPGAVSPSGTREKDIVLQIGLKLAQKVREELGIDA
VMTRSTDVFIELQERTAMANKVGADLFVSVHANASLSRAANGIETYYLNLAKTEKAAQLAAKENGTSLEKVSMLQAVLFD
LMANYKLNDSAHLADEVQKALFKKAQTGYATVKNLGVKQGPFYVLVGATMPSILVETAFLSNDRDEQKLKDPQYQELTAD
GILSGIKGYIASLNKVHAG

Sequences:

>Translated_419_residues
MDRRDFVKYLGLSLPYYQWLSCRMLAAQGSLLLTPFLSAQAGAATAQGLVPVTELKHWSTPDYTRIAITAGSEIPFEHHK
LPNRLYLDLHGAKLNPGVKDLSIGDGLLKSVRIAQFQAATVRVVLDLDSIKDYKVFTFSDPFRIIVDVKGDRRQEISASK
EVIETAPPEPKPTENAKLAEKPKSAGKFKPGRIRRIVIDPGHGGHDPGAVSPSGTREKDIVLQIGLKLAQKVREELGIDA
VMTRSTDVFIELQERTAMANKVGADLFVSVHANASLSRAANGIETYYLNLAKTEKAAQLAAKENGTSLEKVSMLQAVLFD
LMANYKLNDSAHLADEVQKALFKKAQTGYATVKNLGVKQGPFYVLVGATMPSILVETAFLSNDRDEQKLKDPQYQELTAD
GILSGIKGYIASLNKVHAG
>Mature_419_residues
MDRRDFVKYLGLSLPYYQWLSCRMLAAQGSLLLTPFLSAQAGAATAQGLVPVTELKHWSTPDYTRIAITAGSEIPFEHHK
LPNRLYLDLHGAKLNPGVKDLSIGDGLLKSVRIAQFQAATVRVVLDLDSIKDYKVFTFSDPFRIIVDVKGDRRQEISASK
EVIETAPPEPKPTENAKLAEKPKSAGKFKPGRIRRIVIDPGHGGHDPGAVSPSGTREKDIVLQIGLKLAQKVREELGIDA
VMTRSTDVFIELQERTAMANKVGADLFVSVHANASLSRAANGIETYYLNLAKTEKAAQLAAKENGTSLEKVSMLQAVLFD
LMANYKLNDSAHLADEVQKALFKKAQTGYATVKNLGVKQGPFYVLVGATMPSILVETAFLSNDRDEQKLKDPQYQELTAD
GILSGIKGYIASLNKVHAG

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=421, Percent_Identity=32.0665083135392, Blast_Score=174, Evalue=8e-45,
Organism=Escherichia coli, GI1788776, Length=262, Percent_Identity=32.824427480916, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1790611, Length=373, Percent_Identity=29.2225201072386, Blast_Score=121, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 45784; Mature: 45784

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRRDFVKYLGLSLPYYQWLSCRMLAAQGSLLLTPFLSAQAGAATAQGLVPVTELKHWST
CCHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEHHCCCCCCCHHCCCCCHHHHCCCCC
PDYTRIAITAGSEIPFEHHKLPNRLYLDLHGAKLNPGVKDLSIGDGLLKSVRIAQFQAAT
CCCEEEEEEECCCCCHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHEEEEE
VRVVLDLDSIKDYKVFTFSDPFRIIVDVKGDRRQEISASKEVIETAPPEPKPTENAKLAE
EEEEEEECCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHC
KPKSAGKFKPGRIRRIVIDPGHGGHDPGAVSPSGTREKDIVLQIGLKLAQKVREELGIDA
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCE
VMTRSTDVFIELQERTAMANKVGADLFVSVHANASLSRAANGIETYYLNLAKTEKAAQLA
EEECCCEEEEEEHHHHHHHHHCCCEEEEEEECCCCHHHHCCCCEEEEEEEHHHHHHHHHH
AKENGTSLEKVSMLQAVLFDLMANYKLNDSAHLADEVQKALFKKAQTGYATVKNLGVKQG
HHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCC
PFYVLVGATMPSILVETAFLSNDRDEQKLKDPQYQELTADGILSGIKGYIASLNKVHAG
CEEEEECCCCHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDRRDFVKYLGLSLPYYQWLSCRMLAAQGSLLLTPFLSAQAGAATAQGLVPVTELKHWST
CCHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEHHCCCCCCCHHCCCCCHHHHCCCCC
PDYTRIAITAGSEIPFEHHKLPNRLYLDLHGAKLNPGVKDLSIGDGLLKSVRIAQFQAAT
CCCEEEEEEECCCCCHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHEEEEE
VRVVLDLDSIKDYKVFTFSDPFRIIVDVKGDRRQEISASKEVIETAPPEPKPTENAKLAE
EEEEEEECCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHC
KPKSAGKFKPGRIRRIVIDPGHGGHDPGAVSPSGTREKDIVLQIGLKLAQKVREELGIDA
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCE
VMTRSTDVFIELQERTAMANKVGADLFVSVHANASLSRAANGIETYYLNLAKTEKAAQLA
EEECCCEEEEEEHHHHHHHHHCCCEEEEEEECCCCHHHHCCCCEEEEEEEHHHHHHHHHH
AKENGTSLEKVSMLQAVLFDLMANYKLNDSAHLADEVQKALFKKAQTGYATVKNLGVKQG
HHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCC
PFYVLVGATMPSILVETAFLSNDRDEQKLKDPQYQELTADGILSGIKGYIASLNKVHAG
CEEEEECCCCHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]