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The map label for this gene is pstS-3 [H]

Identifier: 197118095

GI number: 197118095

Start: 1976469

End: 1977500

Strand: Direct

Name: pstS-3 [H]

Synonym: Gbem_1710

Alternate gene names: 197118095

Gene position: 1976469-1977500 (Clockwise)

Preceding gene: 197118093

Following gene: 197118096

Centisome position: 42.83

GC content: 59.79

Gene sequence:

>1032_bases
ATGATTCAGCAGATAAAAAAAGCATTCCTGGCGCTGGCCCTCGTGGCGACTGTGGGCGCCGCCGCAGGTCAGGCATCCGC
GGAAACCCTCATTAACGGCGCAGGCGCCACCTTCCCGTATCCCCTTTACTCCAAGTGGTTCAGCGAGTACGCCAAAGTGG
ACAAGAGCGTTAAGTTCAACTACCAATCCATCGGCAGCGGCGGCGGCATCAAGCAGATCACCGCTCAGACCGTCGATTTC
GGCGCCAGCGATAAATTCCTCTCCGATCAGGAACTGAGCGCGGCCCCGGGCAAGCTCCTTCACATCCCGACCGTCATGGG
TGCCGTCGTAGTCACCTACAACGTTCCGGGGGTACCCTCCGGCATCAAGCTCAACTCCGAAGACGTAGCCAACATCTACC
TCGGTAAGATCACCATGTGGAACGACAAGAGGATCGCCGACGACAACAAGGGGATCAACCTCCCGGCCAAGCCGATCATC
GTCGTCCACCGCTCCGACGGCAGCGGCACCACCAGCATCTTTACGGACTACCTGACCGGGGTCAACGCGGAGTGGGCGAA
GAAGGTCGGCAAGGGCGCTTCCGTCAAGTGGCCGGTAGGTCTGGGCGGCAAGGGTAACGAAGGCGTTGCGGGACAGATCA
AGACCACCCCGTACACCATCGGCTACGTCGAACTCGCCTACGCCTTCGAAAACAAGCTTCCCTTCGCGTCCCTGAAAAAC
GGCGCCGGCGTCTTCGTGGCACCGTCCATCAAGTCCACCAGCGCCGCGGCGGCAGCAGCGCTGAAGAATATGCCCGCCGA
CTACCGCATCTCGCTGGTGAACCAGCCCGGCAAGGACGCCTACCCGGTGGTCGGCTTCACCTGGCTCCTCGTTTATGAGA
AGCAGAAGGATGCTGTCAAGGGCAAGAAGCTGGTCGAGTTCCTTAACTGGAGCATGACCAAAGGGCAGAAGATGGCGTCC
CCCATGCTGTACGCGCCGCTTCCGAACAGCGTGGTGAAGATGGTTCAGAAGACGATCAAGACCATCAAGTAA

Upstream 100 bases:

>100_bases
CCTACGCAGGAGGGTGATATTCACAGATTCTTCTGGCCGTTGTAACCAGACTGCAACATTACGGGCCTATAGTCGCTTCA
AAACCGAAAAGGAGGAAGAA

Downstream 100 bases:

>100_bases
AACTGCAGACAATAAAGAGATGACCAGATCCGAGTGTACGGCGTAAAGCCGGCACCCGGATTTTTTTTTAAGGGGGCTTG
GGAAGCAGGTCCCCCCTCCC

Product: phosphate ABC transporter periplasmic phosphate-binding protein

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: PBP [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MIQQIKKAFLALALVATVGAAAGQASAETLINGAGATFPYPLYSKWFSEYAKVDKSVKFNYQSIGSGGGIKQITAQTVDF
GASDKFLSDQELSAAPGKLLHIPTVMGAVVVTYNVPGVPSGIKLNSEDVANIYLGKITMWNDKRIADDNKGINLPAKPII
VVHRSDGSGTTSIFTDYLTGVNAEWAKKVGKGASVKWPVGLGGKGNEGVAGQIKTTPYTIGYVELAYAFENKLPFASLKN
GAGVFVAPSIKSTSAAAAAALKNMPADYRISLVNQPGKDAYPVVGFTWLLVYEKQKDAVKGKKLVEFLNWSMTKGQKMAS
PMLYAPLPNSVVKMVQKTIKTIK

Sequences:

>Translated_343_residues
MIQQIKKAFLALALVATVGAAAGQASAETLINGAGATFPYPLYSKWFSEYAKVDKSVKFNYQSIGSGGGIKQITAQTVDF
GASDKFLSDQELSAAPGKLLHIPTVMGAVVVTYNVPGVPSGIKLNSEDVANIYLGKITMWNDKRIADDNKGINLPAKPII
VVHRSDGSGTTSIFTDYLTGVNAEWAKKVGKGASVKWPVGLGGKGNEGVAGQIKTTPYTIGYVELAYAFENKLPFASLKN
GAGVFVAPSIKSTSAAAAAALKNMPADYRISLVNQPGKDAYPVVGFTWLLVYEKQKDAVKGKKLVEFLNWSMTKGQKMAS
PMLYAPLPNSVVKMVQKTIKTIK
>Mature_343_residues
MIQQIKKAFLALALVATVGAAAGQASAETLINGAGATFPYPLYSKWFSEYAKVDKSVKFNYQSIGSGGGIKQITAQTVDF
GASDKFLSDQELSAAPGKLLHIPTVMGAVVVTYNVPGVPSGIKLNSEDVANIYLGKITMWNDKRIADDNKGINLPAKPII
VVHRSDGSGTTSIFTDYLTGVNAEWAKKVGKGASVKWPVGLGGKGNEGVAGQIKTTPYTIGYVELAYAFENKLPFASLKN
GAGVFVAPSIKSTSAAAAAALKNMPADYRISLVNQPGKDAYPVVGFTWLLVYEKQKDAVKGKKLVEFLNWSMTKGQKMAS
PMLYAPLPNSVVKMVQKTIKTIK

Specific function: Part of the ABC transporter complex pstSACB involved in phosphate import [H]

COG id: COG0226

COG function: function code P; ABC-type phosphate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pstS family [H]

Homologues:

Organism=Escherichia coli, GI2367271, Length=316, Percent_Identity=46.2025316455696, Blast_Score=274, Evalue=7e-75,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005673
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36521; Mature: 36521

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQQIKKAFLALALVATVGAAAGQASAETLINGAGATFPYPLYSKWFSEYAKVDKSVKFN
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEC
YQSIGSGGGIKQITAQTVDFGASDKFLSDQELSAAPGKLLHIPTVMGAVVVTYNVPGVPS
HHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEHHHHHEEEEEEEECCCCCC
GIKLNSEDVANIYLGKITMWNDKRIADDNKGINLPAKPIIVVHRSDGSGTTSIFTDYLTG
CCEECCCCCCEEEEEEEEEECCCEECCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHC
VNAEWAKKVGKGASVKWPVGLGGKGNEGVAGQIKTTPYTIGYVELAYAFENKLPFASLKN
CCHHHHHHHCCCCCEEECCCCCCCCCCCCCEEEECCCEEEEHEEEEEHHHCCCCHHHCCC
GAGVFVAPSIKSTSAAAAAALKNMPADYRISLVNQPGKDAYPVVGFTWLLVYEKQKDAVK
CCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEEEEEEECCHHHHH
GKKLVEFLNWSMTKGQKMASPMLYAPLPNSVVKMVQKTIKTIK
HHHHHHHHHCCCCCCHHHCCCEEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIQQIKKAFLALALVATVGAAAGQASAETLINGAGATFPYPLYSKWFSEYAKVDKSVKFN
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEC
YQSIGSGGGIKQITAQTVDFGASDKFLSDQELSAAPGKLLHIPTVMGAVVVTYNVPGVPS
HHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEHHHHHEEEEEEEECCCCCC
GIKLNSEDVANIYLGKITMWNDKRIADDNKGINLPAKPIIVVHRSDGSGTTSIFTDYLTG
CCEECCCCCCEEEEEEEEEECCCEECCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHC
VNAEWAKKVGKGASVKWPVGLGGKGNEGVAGQIKTTPYTIGYVELAYAFENKLPFASLKN
CCHHHHHHHCCCCCEEECCCCCCCCCCCCCEEEECCCEEEEHEEEEEHHHCCCCHHHCCC
GAGVFVAPSIKSTSAAAAAALKNMPADYRISLVNQPGKDAYPVVGFTWLLVYEKQKDAVK
CCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEEEEEEECCHHHHH
GKKLVEFLNWSMTKGQKMASPMLYAPLPNSVVKMVQKTIKTIK
HHHHHHHHHCCCCCCHHHCCCEEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]