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The map label for this gene is rfbE [H]

Identifier: 197118070

GI number: 197118070

Start: 1948407

End: 1950461

Strand: Direct

Name: rfbE [H]

Synonym: Gbem_1684

Alternate gene names: 197118070

Gene position: 1948407-1950461 (Clockwise)

Preceding gene: 197118069

Following gene: 197118071

Centisome position: 42.22

GC content: 64.77

Gene sequence:

>2055_bases
ATGAAAAGGGCGACCAGTGAAGCGGTACCGGAGCACTTCGGCATAGTGGAGTGGTTCCGGCCGGGCGAGCGGGAACGGGT
GGAACGGGTCCTTTCCGACATGAAGGCGATCGGCGCCAAGAGCCTCAGGACCGGGATCTCCTGGGCGGACTGGTACACGA
GCGAGGGGAAGAGGTGGTACGACTGGCTCATCCCGCGGCTGGCGCGGGAGGTGGAGCTGCTCCCTTGCGTGCTTTACACC
CCCCCCTCCATCGGGATCGAGGCGAAGACCTCATCCCCCCCGCGTCGTCCCAAGGATTACGCCGACTTCATCGACCTTTT
CGTCACCGCCTTCGGGGAGCATTTCGAGTACCTGGAGCTCTGGAACGAGCCTAACAACCTGAGCGAGTGGGACTGGACCC
TGGACCCGCACTGGACCTCCTTCGGGGAGATGATCGGCGGGGCGGCCTACTGGGTCAGAAAGCGCGGCAAGAAGACCGTC
CTCGGGGGGATGAGCCCCATCGACGGCCACTGGCTCTGCCGGATGTTCGAGCTGGGGGTGATGGATTACATCGACGTGGT
GGGAATCCACGGTTTCCCGGACATCTTCGATTACACCTGGAAGGGGTGGCAGCGCAACATCGCCATGGTGCGGGAGATCC
TCGACGAGAAGGAGTGCGCCTGCGAGATCTGGGTCACCGAGGCGGGCTTCTCCACCTGGCAGCACGACGAGTTCAAGCAG
GCCAAGGTCTTCCTCGATTTTCTCGCCGCCCCGGCGCAGCGCGTCTACTGGTACGGCGTGGACGATCTCGATCCCTCGCT
TTCCGCGGTGGACCGCTACCATCTGGACGAGCGCGAGTACTTTTTCGGCTTGAGGAAGGCGGACGCAACGCCCAAGCTCC
TCTACCGCCTGCTCCAGGAGGGGACGCTTTCCTCCCTGAAGCGCGTGGTAGCCGCTGGGAGCGCCGCGAGGGCAGACAGC
GGCGGGACCGAAAAGGCGGTGCTCGTCACCGGGGGGGCCGGGTTCATCGGGACCAACCTGGTGCAGCACCTGGTGGCGCA
GGGGGAGAGGGTGATCCTCTACGACAACCTCTCCCGCGCAGGGGTCGAGAAGAATCTCCTCTGGCTCATGGACAACTGCG
GCGAAAGGCTGCAGGTGGTGATAGGGGACACCCGCAACTCTCTCCTTCTGGAGCAGGCGGTAAGTGAGGCGAAACAGGTC
TTCCACTTCGCGGCCCAGGTAGCGGTCTCCAGCAGTATCGACAACCCCGCCAACGACTTCGCCATCAACGTCCAGGGGAC
CTTCTCGCTCCTGGAGGCGATCCGCAAGGCGAAGACCCCTCCTTCGCTTCTCTACACCTCCACCAACAAGGTGTACGGGG
CCATCGAGGGGTGCGGCGTCCGGAAAAACGGGGTGCGCTACGAGCCGCTCGACCCGCAGCTCCGCTCCCACGGGCTGGGA
GAAGGGACCACGCTCGATTTCCTGAGCCCCTACGGCTGCTCCAAGGGATGCGCCGACCAATATGTCCTGGACTACGCCCG
CAGCTTCGGCATCGACGCCGCGGTCTTCCGGATGAGCTGCATCTACGGCCCGCACCAGTACGGCACCGAGGAACAGGGGT
GGGTGGCGCACTTCGCCATACAGACCATGAAAGGGGAGCCCATCACCCTCTACGGGGACGGCTGCCAAATCCGGGATCTC
CTCTTCGTCGAGGACCTGGTGGACGCCATGTGCCGGGCGCGGGACATCATGCCGCGCATAGCCGGCCAGGCCTTCAACAT
CGGCGGCGGCCCCGCTCGCACCATAAGCCTCTTGGAGCTTTTGGATCTGTTGCGCGATCTGCACGGCACCCTTCCCACCA
TACTGCGCGACGACTGGCGCACCGGGGACCAGAGGTACTACGTCTCCGACACCAGGAAGTTCTGCAAGGCTACCGGATGG
ACGCCGCGGCATTCGGTGGCCGAGGGGGTGCGCAGGCTGTACGACTGGCTCCTGGAAACGATGCACTCGCCGGCGCGCGG
CGCCGGGAGCTTCGACAAGCAAAGCTACCCGGCGACGGGGGCGGAGGCGATCTGA

Upstream 100 bases:

>100_bases
ACGATGGGCTTGCCGAACTGGCCGGCTGGCTGGAAGGGGAGGTCGCCATAGACCGTGTCTCCGAGGCGCATGCCGAACTC
ACCCAGCGGGGGTTGACGCT

Downstream 100 bases:

>100_bases
TGAGCGATGTGATGGATGCTGCGGTAATCACCGGTCCTGGGCGGTCGCGGGTAGAACAGGTGGCGCGCCCTGCGGCCGGC
AGGGGGGAAGTAGTGGTGCG

Product: NAD-dependent nucleoside diphosphate-sugar epimerase/dehydratase

Products: NA

Alternate protein names: CDP-tyvelose 2-epimerase [H]

Number of amino acids: Translated: 684; Mature: 684

Protein sequence:

>684_residues
MKRATSEAVPEHFGIVEWFRPGERERVERVLSDMKAIGAKSLRTGISWADWYTSEGKRWYDWLIPRLAREVELLPCVLYT
PPSIGIEAKTSSPPRRPKDYADFIDLFVTAFGEHFEYLELWNEPNNLSEWDWTLDPHWTSFGEMIGGAAYWVRKRGKKTV
LGGMSPIDGHWLCRMFELGVMDYIDVVGIHGFPDIFDYTWKGWQRNIAMVREILDEKECACEIWVTEAGFSTWQHDEFKQ
AKVFLDFLAAPAQRVYWYGVDDLDPSLSAVDRYHLDEREYFFGLRKADATPKLLYRLLQEGTLSSLKRVVAAGSAARADS
GGTEKAVLVTGGAGFIGTNLVQHLVAQGERVILYDNLSRAGVEKNLLWLMDNCGERLQVVIGDTRNSLLLEQAVSEAKQV
FHFAAQVAVSSSIDNPANDFAINVQGTFSLLEAIRKAKTPPSLLYTSTNKVYGAIEGCGVRKNGVRYEPLDPQLRSHGLG
EGTTLDFLSPYGCSKGCADQYVLDYARSFGIDAAVFRMSCIYGPHQYGTEEQGWVAHFAIQTMKGEPITLYGDGCQIRDL
LFVEDLVDAMCRARDIMPRIAGQAFNIGGGPARTISLLELLDLLRDLHGTLPTILRDDWRTGDQRYYVSDTRKFCKATGW
TPRHSVAEGVRRLYDWLLETMHSPARGAGSFDKQSYPATGAEAI

Sequences:

>Translated_684_residues
MKRATSEAVPEHFGIVEWFRPGERERVERVLSDMKAIGAKSLRTGISWADWYTSEGKRWYDWLIPRLAREVELLPCVLYT
PPSIGIEAKTSSPPRRPKDYADFIDLFVTAFGEHFEYLELWNEPNNLSEWDWTLDPHWTSFGEMIGGAAYWVRKRGKKTV
LGGMSPIDGHWLCRMFELGVMDYIDVVGIHGFPDIFDYTWKGWQRNIAMVREILDEKECACEIWVTEAGFSTWQHDEFKQ
AKVFLDFLAAPAQRVYWYGVDDLDPSLSAVDRYHLDEREYFFGLRKADATPKLLYRLLQEGTLSSLKRVVAAGSAARADS
GGTEKAVLVTGGAGFIGTNLVQHLVAQGERVILYDNLSRAGVEKNLLWLMDNCGERLQVVIGDTRNSLLLEQAVSEAKQV
FHFAAQVAVSSSIDNPANDFAINVQGTFSLLEAIRKAKTPPSLLYTSTNKVYGAIEGCGVRKNGVRYEPLDPQLRSHGLG
EGTTLDFLSPYGCSKGCADQYVLDYARSFGIDAAVFRMSCIYGPHQYGTEEQGWVAHFAIQTMKGEPITLYGDGCQIRDL
LFVEDLVDAMCRARDIMPRIAGQAFNIGGGPARTISLLELLDLLRDLHGTLPTILRDDWRTGDQRYYVSDTRKFCKATGW
TPRHSVAEGVRRLYDWLLETMHSPARGAGSFDKQSYPATGAEAI
>Mature_684_residues
MKRATSEAVPEHFGIVEWFRPGERERVERVLSDMKAIGAKSLRTGISWADWYTSEGKRWYDWLIPRLAREVELLPCVLYT
PPSIGIEAKTSSPPRRPKDYADFIDLFVTAFGEHFEYLELWNEPNNLSEWDWTLDPHWTSFGEMIGGAAYWVRKRGKKTV
LGGMSPIDGHWLCRMFELGVMDYIDVVGIHGFPDIFDYTWKGWQRNIAMVREILDEKECACEIWVTEAGFSTWQHDEFKQ
AKVFLDFLAAPAQRVYWYGVDDLDPSLSAVDRYHLDEREYFFGLRKADATPKLLYRLLQEGTLSSLKRVVAAGSAARADS
GGTEKAVLVTGGAGFIGTNLVQHLVAQGERVILYDNLSRAGVEKNLLWLMDNCGERLQVVIGDTRNSLLLEQAVSEAKQV
FHFAAQVAVSSSIDNPANDFAINVQGTFSLLEAIRKAKTPPSLLYTSTNKVYGAIEGCGVRKNGVRYEPLDPQLRSHGLG
EGTTLDFLSPYGCSKGCADQYVLDYARSFGIDAAVFRMSCIYGPHQYGTEEQGWVAHFAIQTMKGEPITLYGDGCQIRDL
LFVEDLVDAMCRARDIMPRIAGQAFNIGGGPARTISLLELLDLLRDLHGTLPTILRDDWRTGDQRYYVSDTRKFCKATGW
TPRHSVAEGVRRLYDWLLETMHSPARGAGSFDKQSYPATGAEAI

Specific function: Catalyzes the isomeration of CDP-paratose to CDP- tyvelose [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=353, Percent_Identity=26.628895184136, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI42516563, Length=337, Percent_Identity=25.2225519287834, Blast_Score=107, Evalue=5e-23,
Organism=Homo sapiens, GI56237023, Length=355, Percent_Identity=25.3521126760563, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI56118217, Length=355, Percent_Identity=25.3521126760563, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI189083684, Length=355, Percent_Identity=25.3521126760563, Blast_Score=73, Evalue=7e-13,
Organism=Escherichia coli, GI1788353, Length=351, Percent_Identity=29.9145299145299, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI48994969, Length=359, Percent_Identity=29.5264623955432, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1786974, Length=351, Percent_Identity=25.6410256410256, Blast_Score=90, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17539532, Length=355, Percent_Identity=26.1971830985915, Blast_Score=115, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17568069, Length=358, Percent_Identity=27.3743016759777, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI115532424, Length=346, Percent_Identity=26.3005780346821, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21356223, Length=353, Percent_Identity=25.2124645892351, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19923002, Length=356, Percent_Identity=26.685393258427, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.10 [H]

Molecular weight: Translated: 76940; Mature: 76940

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRATSEAVPEHFGIVEWFRPGERERVERVLSDMKAIGAKSLRTGISWADWYTSEGKRWY
CCCCCHHCCHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHH
DWLIPRLAREVELLPCVLYTPPSIGIEAKTSSPPRRPKDYADFIDLFVTAFGEHFEYLEL
HHHHHHHHHHHHHCEEEEECCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
WNEPNNLSEWDWTLDPHWTSFGEMIGGAAYWVRKRGKKTVLGGMSPIDGHWLCRMFELGV
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHH
MDYIDVVGIHGFPDIFDYTWKGWQRNIAMVREILDEKECACEIWVTEAGFSTWQHDEFKQ
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHH
AKVFLDFLAAPAQRVYWYGVDDLDPSLSAVDRYHLDEREYFFGLRKADATPKLLYRLLQE
HHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHC
GTLSSLKRVVAAGSAARADSGGTEKAVLVTGGAGFIGTNLVQHLVAQGERVILYDNLSRA
CCHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCC
GVEKNLLWLMDNCGERLQVVIGDTRNSLLLEQAVSEAKQVFHFAAQVAVSSSIDNPANDF
CCCCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
AINVQGTFSLLEAIRKAKTPPSLLYTSTNKVYGAIEGCGVRKNGVRYEPLDPQLRSHGLG
EEEECHHHHHHHHHHHCCCCCCEEEECCCCEEECCCCCCCCCCCCCCCCCCHHHHHCCCC
EGTTLDFLSPYGCSKGCADQYVLDYARSFGIDAAVFRMSCIYGPHQYGTEEQGWVAHFAI
CCCEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHCCCCCCCCEEEEEE
QTMKGEPITLYGDGCQIRDLLFVEDLVDAMCRARDIMPRIAGQAFNIGGGPARTISLLEL
EECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHH
LDLLRDLHGTLPTILRDDWRTGDQRYYVSDTRKFCKATGWTPRHSVAEGVRRLYDWLLET
HHHHHHHHCCHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
MHSPARGAGSFDKQSYPATGAEAI
HCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKRATSEAVPEHFGIVEWFRPGERERVERVLSDMKAIGAKSLRTGISWADWYTSEGKRWY
CCCCCHHCCHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHH
DWLIPRLAREVELLPCVLYTPPSIGIEAKTSSPPRRPKDYADFIDLFVTAFGEHFEYLEL
HHHHHHHHHHHHHCEEEEECCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
WNEPNNLSEWDWTLDPHWTSFGEMIGGAAYWVRKRGKKTVLGGMSPIDGHWLCRMFELGV
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHH
MDYIDVVGIHGFPDIFDYTWKGWQRNIAMVREILDEKECACEIWVTEAGFSTWQHDEFKQ
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHH
AKVFLDFLAAPAQRVYWYGVDDLDPSLSAVDRYHLDEREYFFGLRKADATPKLLYRLLQE
HHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHC
GTLSSLKRVVAAGSAARADSGGTEKAVLVTGGAGFIGTNLVQHLVAQGERVILYDNLSRA
CCHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCC
GVEKNLLWLMDNCGERLQVVIGDTRNSLLLEQAVSEAKQVFHFAAQVAVSSSIDNPANDF
CCCCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
AINVQGTFSLLEAIRKAKTPPSLLYTSTNKVYGAIEGCGVRKNGVRYEPLDPQLRSHGLG
EEEECHHHHHHHHHHHCCCCCCEEEECCCCEEECCCCCCCCCCCCCCCCCCHHHHHCCCC
EGTTLDFLSPYGCSKGCADQYVLDYARSFGIDAAVFRMSCIYGPHQYGTEEQGWVAHFAI
CCCEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHCCCCCCCCEEEEEE
QTMKGEPITLYGDGCQIRDLLFVEDLVDAMCRARDIMPRIAGQAFNIGGGPARTISLLEL
EECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHH
LDLLRDLHGTLPTILRDDWRTGDQRYYVSDTRKFCKATGWTPRHSVAEGVRRLYDWLLET
HHHHHHHHCCHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
MHSPARGAGSFDKQSYPATGAEAI
HCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2793833; 11677608; 12644504; 12642575 [H]