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The map label for this gene is treZ [H]

Identifier: 197118068

GI number: 197118068

Start: 1945084

End: 1946964

Strand: Reverse

Name: treZ [H]

Synonym: Gbem_1682

Alternate gene names: 197118068

Gene position: 1946964-1945084 (Counterclockwise)

Preceding gene: 197118089

Following gene: 197118067

Centisome position: 42.19

GC content: 64.7

Gene sequence:

>1881_bases
ATGACTGCGCTACAAAGACGGCTGGCCATGGGAGCGGAGATTGTCCCGGAAGGTGTCCACTTCCGAGTTTGGGCCCCGGC
GCACAGGAAGGTGGAGGTGGTGCTGGAGGGAGGGGCCGGCCAGGGGAGTTCGGTGGAGCTGGAATCGGAAGAGGAAGGTT
TTTTTGCCGGATTAAGCCGCGAGGCGCGGGCGGGGTCGTTGTACCGCTATCGGCTGGACGAGGCAATGAACCTCCACCCC
GATCCCGCCTCGCGCTTCCAGCCCGACGGTCCGCACGGCCCCTCGCAGGTGGTCGACCCTTCCAAATACAGGTGGAGCGA
TGGCGAATGGCCCGGGGTCGTGCGCGAGGGGCACGTGATCTACGAGATGCACCTGGGGACCTATACCCAGGAAGGGAGTT
GGCGGGCGGCGGCGGAGCAGCTCCAGGAACTGAAGGAGTTGGGGATCACCCTGGTAGAGGTGATGCCGGTGGCGGATTTC
CCCGGCAGGTTCGGCTGGGGGTACGACGGGGTGAACCACTTCGCCCCCACGAGGCTTTACGGCGAGCCGGACGACATGCG
CAGCTTCGTGGACAAGGCCCACCGCCTGGGGCTCGGAGTGTTGCTGGACGTGGTCTACAACCACTTCGGCCCGGAAGGGA
ACTACCTGGCCCAGTTCTCCCAGTACTACTACGCGGAAGAGGACGGCGAATGGGGCAAGGCGATGAACTTCGACGGCAGG
CGCAGCCGCCCGGTAAGGGAGTTCTTCGCCAGCAACGCCGCCTACTGGATCGACGAGTTCCACCTGGACGGCCTGCGCTT
CGACGCCACCCACGCCATTCACGACGACTCCCCCATCCACGTCCTGGGGGAGATCACCGAAAAGGCGCGGATAGCCGCCG
GCAGGCGCGCCATCTTACTAGTGGCCGAGAGCGAGAACCAGGACGCGCGCTGCCTGCGCGATAAGGAAGCTGGCGGCTTC
GGCATGGACGCGGTCTGGAACGACGACTTCCACCACAGCGCCTACGTGGCGCTCACCGGCTACCACGACGCCTACTACTC
GGAATACTTCGGCTCGCCGCAGGAGCTGGCCTCGGCGGCCAAGTGGGGCTACCTGTACCAGGGGCAGTTCTACTTCTGGC
AGGGGAAAAGGCGCGGCTCCCCAACCATCGGCGTGAGCCCTGCCTGCTTCGTCAACTACATCCAAAACCACGACCAGATC
GGCAACTCCGCCTGGGGGATGAGGATCGACCGGCTCACGAACCCTGCGGCGCTCCGGACCATGACGGCGTTCCTGATGCT
CGCCCCGCAGACCCCGATGATCTTCCAGGGGCAGGAGTTTTCCGCAAGCTCTCCATTTATGTACTTCGCGGACCTGAGCC
CGGAGATCTCGCAGGCGGTGCACGCCGGCCGAATCGAGTACCTGAAGCAGTTCACCAACATAGACGCCCCGGAGATCATC
GACACGATAGACAAGCCGTACGAGGTGAAGACCTTCGAGCAGTCGCGGCTCCACTTAAAGGAGCGCGAGCGGCACGCGAA
GACCTACGCGCTCTACCGCGACCTGATCCGGCTGCGGCGCGAGGACCGGGTCTTCAGCCGCGGCTATGCCTGCCATATCG
AGGGGGCGGTGCTGGGGGCGAGCGCCTTTTTGCTCCGCTATTTCCTGAAGGGGGACCAGCGCCTGCTCCTGATCAACCTG
GGGCGCGAGCTGCACCTGGTGCCGATCCCGGAGCCTATGCTGGCGCCGCCGGAGGGATGCGACTGGGAGATCCTGTGGAC
CAGCGAGAAGCTGGAATTCGGCGGCTCGGGAACGCCGAAGCTCGACACGGAGAAATTTTGGCGCATACAGGGGAATGCGG
CGGTGGTGCTGATTCCGGTCCGCCAGGGAGAAGAGCCATGA

Upstream 100 bases:

>100_bases
AGTCTTGGATGAGCGACAAGAAACCGGCAGGTGCGGTTTTAAAATTTGCGAACTATGTTATTTTTAGAGCCTGTTAATTT
TAATAAGACCGGAGGTAGCG

Downstream 100 bases:

>100_bases
CCACAGTGACACGCGACTTCCATTTCGACCGGCATGACGAGAAGACCAAGACGCGGCAGCTCCTGGAGCAGGAGTGGCTT
CTCACCAACGGCCTGGGAGG

Product: alpha-glucan branching enzyme

Products: NA

Alternate protein names: MTHase; 4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase; Maltooligosyl trehalose trehalohydrolase [H]

Number of amino acids: Translated: 626; Mature: 625

Protein sequence:

>626_residues
MTALQRRLAMGAEIVPEGVHFRVWAPAHRKVEVVLEGGAGQGSSVELESEEEGFFAGLSREARAGSLYRYRLDEAMNLHP
DPASRFQPDGPHGPSQVVDPSKYRWSDGEWPGVVREGHVIYEMHLGTYTQEGSWRAAAEQLQELKELGITLVEVMPVADF
PGRFGWGYDGVNHFAPTRLYGEPDDMRSFVDKAHRLGLGVLLDVVYNHFGPEGNYLAQFSQYYYAEEDGEWGKAMNFDGR
RSRPVREFFASNAAYWIDEFHLDGLRFDATHAIHDDSPIHVLGEITEKARIAAGRRAILLVAESENQDARCLRDKEAGGF
GMDAVWNDDFHHSAYVALTGYHDAYYSEYFGSPQELASAAKWGYLYQGQFYFWQGKRRGSPTIGVSPACFVNYIQNHDQI
GNSAWGMRIDRLTNPAALRTMTAFLMLAPQTPMIFQGQEFSASSPFMYFADLSPEISQAVHAGRIEYLKQFTNIDAPEII
DTIDKPYEVKTFEQSRLHLKERERHAKTYALYRDLIRLRREDRVFSRGYACHIEGAVLGASAFLLRYFLKGDQRLLLINL
GRELHLVPIPEPMLAPPEGCDWEILWTSEKLEFGGSGTPKLDTEKFWRIQGNAAVVLIPVRQGEEP

Sequences:

>Translated_626_residues
MTALQRRLAMGAEIVPEGVHFRVWAPAHRKVEVVLEGGAGQGSSVELESEEEGFFAGLSREARAGSLYRYRLDEAMNLHP
DPASRFQPDGPHGPSQVVDPSKYRWSDGEWPGVVREGHVIYEMHLGTYTQEGSWRAAAEQLQELKELGITLVEVMPVADF
PGRFGWGYDGVNHFAPTRLYGEPDDMRSFVDKAHRLGLGVLLDVVYNHFGPEGNYLAQFSQYYYAEEDGEWGKAMNFDGR
RSRPVREFFASNAAYWIDEFHLDGLRFDATHAIHDDSPIHVLGEITEKARIAAGRRAILLVAESENQDARCLRDKEAGGF
GMDAVWNDDFHHSAYVALTGYHDAYYSEYFGSPQELASAAKWGYLYQGQFYFWQGKRRGSPTIGVSPACFVNYIQNHDQI
GNSAWGMRIDRLTNPAALRTMTAFLMLAPQTPMIFQGQEFSASSPFMYFADLSPEISQAVHAGRIEYLKQFTNIDAPEII
DTIDKPYEVKTFEQSRLHLKERERHAKTYALYRDLIRLRREDRVFSRGYACHIEGAVLGASAFLLRYFLKGDQRLLLINL
GRELHLVPIPEPMLAPPEGCDWEILWTSEKLEFGGSGTPKLDTEKFWRIQGNAAVVLIPVRQGEEP
>Mature_625_residues
TALQRRLAMGAEIVPEGVHFRVWAPAHRKVEVVLEGGAGQGSSVELESEEEGFFAGLSREARAGSLYRYRLDEAMNLHPD
PASRFQPDGPHGPSQVVDPSKYRWSDGEWPGVVREGHVIYEMHLGTYTQEGSWRAAAEQLQELKELGITLVEVMPVADFP
GRFGWGYDGVNHFAPTRLYGEPDDMRSFVDKAHRLGLGVLLDVVYNHFGPEGNYLAQFSQYYYAEEDGEWGKAMNFDGRR
SRPVREFFASNAAYWIDEFHLDGLRFDATHAIHDDSPIHVLGEITEKARIAAGRRAILLVAESENQDARCLRDKEAGGFG
MDAVWNDDFHHSAYVALTGYHDAYYSEYFGSPQELASAAKWGYLYQGQFYFWQGKRRGSPTIGVSPACFVNYIQNHDQIG
NSAWGMRIDRLTNPAALRTMTAFLMLAPQTPMIFQGQEFSASSPFMYFADLSPEISQAVHAGRIEYLKQFTNIDAPEIID
TIDKPYEVKTFEQSRLHLKERERHAKTYALYRDLIRLRREDRVFSRGYACHIEGAVLGASAFLLRYFLKGDQRLLLINLG
RELHLVPIPEPMLAPPEGCDWEILWTSEKLEFGGSGTPKLDTEKFWRIQGNAAVVLIPVRQGEEP

Specific function: Catalyzes The Formation Of The Alpha-1,6-Glucosidic Linkages In Glycogen By Scission Of A 1,4-Alpha-Linked Oligosaccharide From Growing Alpha-1,4-Glucan Chains And The Subsequent Attachment Of The Oligosaccharide To The Alpha-1,6 Position. Has A Preferenc

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=167, Percent_Identity=30.5389221556886, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1789839, Length=280, Percent_Identity=31.0714285714286, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI2367229, Length=332, Percent_Identity=25.6024096385542, Blast_Score=85, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17554896, Length=180, Percent_Identity=30.5555555555556, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI32564391, Length=180, Percent_Identity=30.5555555555556, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320826, Length=167, Percent_Identity=28.7425149700599, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI28573410, Length=213, Percent_Identity=29.1079812206573, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- InterPro:   IPR015156
- InterPro:   IPR012768 [H]

Pfam domain/function: PF09071 Alpha-amyl_C; PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: =3.2.1.141 [H]

Molecular weight: Translated: 70908; Mature: 70777

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALQRRLAMGAEIVPEGVHFRVWAPAHRKVEVVLEGGAGQGSSVELESEEEGFFAGLSR
CCHHHHHHHCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCEEEECCCCCCCEECCCC
EARAGSLYRYRLDEAMNLHPDPASRFQPDGPHGPSQVVDPSKYRWSDGEWPGVVREGHVI
CCCCCCEEEEEHHHHCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCEEE
YEMHLGTYTQEGSWRAAAEQLQELKELGITLVEVMPVADFPGRFGWGYDGVNHFAPTRLY
EEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
GEPDDMRSFVDKAHRLGLGVLLDVVYNHFGPEGNYLAQFSQYYYAEEDGEWGKAMNFDGR
CCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHEEECCCCCCCCCCCCCCC
RSRPVREFFASNAAYWIDEFHLDGLRFDATHAIHDDSPIHVLGEITEKARIAAGRRAILL
CCCHHHHHHHCCCEEEEEEEECCCEEECCEEECCCCCCEEEHHHHHHHHHHHCCCEEEEE
VAESENQDARCLRDKEAGGFGMDAVWNDDFHHSAYVALTGYHDAYYSEYFGSPQELASAA
EECCCCCCHHHHHHCCCCCCCCCCEECCCCCCEEEEEEECCCHHHHHHHCCCHHHHHHHH
KWGYLYQGQFYFWQGKRRGSPTIGVSPACFVNYIQNHDQIGNSAWGMRIDRLTNPAALRT
HCCEEEECEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHH
MTAFLMLAPQTPMIFQGQEFSASSPFMYFADLSPEISQAVHAGRIEYLKQFTNIDAPEII
HHHHHHCCCCCCEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHH
DTIDKPYEVKTFEQSRLHLKERERHAKTYALYRDLIRLRREDRVFSRGYACHIEGAVLGA
HHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHH
SAFLLRYFLKGDQRLLLINLGRELHLVPIPEPMLAPPEGCDWEILWTSEKLEFGGSGTPK
HHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCEEEEEECCEEECCCCCCCC
LDTEKFWRIQGNAAVVLIPVRQGEEP
CCCCCEEEEECCEEEEEEEECCCCCC
>Mature Secondary Structure 
TALQRRLAMGAEIVPEGVHFRVWAPAHRKVEVVLEGGAGQGSSVELESEEEGFFAGLSR
CHHHHHHHCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCEEEECCCCCCCEECCCC
EARAGSLYRYRLDEAMNLHPDPASRFQPDGPHGPSQVVDPSKYRWSDGEWPGVVREGHVI
CCCCCCEEEEEHHHHCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCEEE
YEMHLGTYTQEGSWRAAAEQLQELKELGITLVEVMPVADFPGRFGWGYDGVNHFAPTRLY
EEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
GEPDDMRSFVDKAHRLGLGVLLDVVYNHFGPEGNYLAQFSQYYYAEEDGEWGKAMNFDGR
CCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHEEECCCCCCCCCCCCCCC
RSRPVREFFASNAAYWIDEFHLDGLRFDATHAIHDDSPIHVLGEITEKARIAAGRRAILL
CCCHHHHHHHCCCEEEEEEEECCCEEECCEEECCCCCCEEEHHHHHHHHHHHCCCEEEEE
VAESENQDARCLRDKEAGGFGMDAVWNDDFHHSAYVALTGYHDAYYSEYFGSPQELASAA
EECCCCCCHHHHHHCCCCCCCCCCEECCCCCCEEEEEEECCCHHHHHHHCCCHHHHHHHH
KWGYLYQGQFYFWQGKRRGSPTIGVSPACFVNYIQNHDQIGNSAWGMRIDRLTNPAALRT
HCCEEEECEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHH
MTAFLMLAPQTPMIFQGQEFSASSPFMYFADLSPEISQAVHAGRIEYLKQFTNIDAPEII
HHHHHHCCCCCCEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHH
DTIDKPYEVKTFEQSRLHLKERERHAKTYALYRDLIRLRREDRVFSRGYACHIEGAVLGA
HHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHH
SAFLLRYFLKGDQRLLLINLGRELHLVPIPEPMLAPPEGCDWEILWTSEKLEFGGSGTPK
HHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCEEEEEECCEEECCCCCCCC
LDTEKFWRIQGNAAVVLIPVRQGEEP
CCCCCEEEEECCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8899719; 11427726 [H]