Click here to switch to the map view.

The map label for this gene is hprA [H]

Identifier: 197118035

GI number: 197118035

Start: 1913328

End: 1914293

Strand: Direct

Name: hprA [H]

Synonym: Gbem_1648

Alternate gene names: 197118035

Gene position: 1913328-1914293 (Clockwise)

Preceding gene: 197118034

Following gene: 197118037

Centisome position: 41.46

GC content: 66.05

Gene sequence:

>966_bases
ATGAAGATAGTGGTCCTGGATGGATATACGCTGAACCCGGGCGACAACCCCTGGGACGAGGTCGCCTCCTTCGGCGACCT
CACGCTCTACGACCGCACCCCTGCCGAGATGGTGGTGGAGCGCTCCCGCGGAGCGCAGGTGCTCCTTACCAACAAGACGG
TGCTCTCGGCGGCGACCTTGGCTGCGCTCCCGGAACTGAGGCTCGTCTGCGTGCTGGCGACCGGCTACAACGTGGTCGAC
CTGGAAAAGAGCGCGCAGCTGGGGATACCGGTGGTCAACGTCCCGGAGTACGGCAGCGACTCGGTGGCCCAGCACGCCAT
AGCCCTCCTCCTGGAACTCACCAACCGGGTGGCGCAGTACCATCAGGCCGTGGCCCGCGGCGATTGGTCCGCCTCTCCCG
ACTTCACCCTGGTGGGAGAGCCTTTGACGGAACTTTGCGGCAGGAGCATCGGCATCGTGGGGCTCGGGAGGATAGGGGCG
CGGGTGGCGCGCATCGCACAGGCGCTGGGGATGGAGGTTCTGGCTTACAACCCGAGGCACCGGGTGGCCCCGGAAGGGAT
CTCTTTGCGCTGGCTTAGTCTGGACCAGCTTTTCAGCCAGGCCGACGTGGTGAGCCTGCACTGCCCGCTCAATGATGAAA
ACGAAGGGATGGTGAACCAAAGGACCCTGTCGCTGATGAAGCCGCAGGCGCTCCTTATCAACACCTCCCGCGGCGGGCTC
GTCGTCGAGCGCGACCTAGCCGAGGCTTTGAACCGCGGGAGCATCGCCGGCGCCGCGGTCGACGTCGCCGCTAGGGAGCC
GATACCCGCGGACAGCCCGCTTTTGCTGGCCAAGAACTGCATCGTCACCCCGCATATCGCCTGGGCGACACTTGCCGCCC
GGCGCAGGCTGATGGCGGCGACCGCCGCCAACATCGCCTCCTTCCTGGCGGGGAAACCGCAAAACGTGGTGAACGGGGTG
CAGTGA

Upstream 100 bases:

>100_bases
TTTTTCAAGGAAGCTGAGGTGCACGACTGCGGCATGTCGGTCGAGGACGCGGTCAAGTTCGTGGTCTCGGGCGGGGCCGT
GGTGCAGTAAGGGAGGCATT

Downstream 100 bases:

>100_bases
TAGCGCTTGGGAACCCATGCGGCGCAAACCTCCAGCTTTGCCTCCGTGGGAAGCTGGAGCTTCGCAGGGTCCTTGCGTTC
CCAAGGTGGACCTTGGGAAC

Product: hydroxypyruvate reductase

Products: NA

Alternate protein names: GDH; Glyoxylate reductase; Hydroxypyruvate dehydrogenase; NADH-dependent hydroxypyruvate reductase; HPR; HPR-A [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKIVVLDGYTLNPGDNPWDEVASFGDLTLYDRTPAEMVVERSRGAQVLLTNKTVLSAATLAALPELRLVCVLATGYNVVD
LEKSAQLGIPVVNVPEYGSDSVAQHAIALLLELTNRVAQYHQAVARGDWSASPDFTLVGEPLTELCGRSIGIVGLGRIGA
RVARIAQALGMEVLAYNPRHRVAPEGISLRWLSLDQLFSQADVVSLHCPLNDENEGMVNQRTLSLMKPQALLINTSRGGL
VVERDLAEALNRGSIAGAAVDVAAREPIPADSPLLLAKNCIVTPHIAWATLAARRRLMAATAANIASFLAGKPQNVVNGV
Q

Sequences:

>Translated_321_residues
MKIVVLDGYTLNPGDNPWDEVASFGDLTLYDRTPAEMVVERSRGAQVLLTNKTVLSAATLAALPELRLVCVLATGYNVVD
LEKSAQLGIPVVNVPEYGSDSVAQHAIALLLELTNRVAQYHQAVARGDWSASPDFTLVGEPLTELCGRSIGIVGLGRIGA
RVARIAQALGMEVLAYNPRHRVAPEGISLRWLSLDQLFSQADVVSLHCPLNDENEGMVNQRTLSLMKPQALLINTSRGGL
VVERDLAEALNRGSIAGAAVDVAAREPIPADSPLLLAKNCIVTPHIAWATLAARRRLMAATAANIASFLAGKPQNVVNGV
Q
>Mature_321_residues
MKIVVLDGYTLNPGDNPWDEVASFGDLTLYDRTPAEMVVERSRGAQVLLTNKTVLSAATLAALPELRLVCVLATGYNVVD
LEKSAQLGIPVVNVPEYGSDSVAQHAIALLLELTNRVAQYHQAVARGDWSASPDFTLVGEPLTELCGRSIGIVGLGRIGA
RVARIAQALGMEVLAYNPRHRVAPEGISLRWLSLDQLFSQADVVSLHCPLNDENEGMVNQRTLSLMKPQALLINTSRGGL
VVERDLAEALNRGSIAGAAVDVAAREPIPADSPLLLAKNCIVTPHIAWATLAARRRLMAATAANIASFLAGKPQNVVNGV
Q

Specific function: Plays a central role in assimilation of carbon. It converts hydroxypyruvate to glycerate as a key step in the serine cycle, and may also play an important role in C2 reactions, by interconverting glyoxylate and glycolate [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI145580578, Length=291, Percent_Identity=34.7079037800687, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI4557499, Length=291, Percent_Identity=34.7079037800687, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI61743967, Length=290, Percent_Identity=34.8275862068965, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI4557497, Length=290, Percent_Identity=34.8275862068965, Blast_Score=151, Evalue=6e-37,
Organism=Homo sapiens, GI145580575, Length=293, Percent_Identity=34.4709897610922, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI6912396, Length=255, Percent_Identity=33.3333333333333, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI23308577, Length=287, Percent_Identity=31.7073170731707, Blast_Score=135, Evalue=6e-32,
Organism=Escherichia coli, GI87082289, Length=280, Percent_Identity=34.6428571428571, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1787645, Length=309, Percent_Identity=29.4498381877023, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI1789279, Length=280, Percent_Identity=34.6428571428571, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI87081824, Length=202, Percent_Identity=29.2079207920792, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17532191, Length=289, Percent_Identity=35.2941176470588, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI25147481, Length=283, Percent_Identity=30.3886925795053, Blast_Score=125, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6324964, Length=315, Percent_Identity=28.8888888888889, Blast_Score=126, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6320925, Length=289, Percent_Identity=29.757785467128, Blast_Score=119, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6324055, Length=327, Percent_Identity=26.2996941896024, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6322116, Length=289, Percent_Identity=29.4117647058824, Blast_Score=116, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6324980, Length=199, Percent_Identity=30.1507537688442, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24646446, Length=289, Percent_Identity=34.9480968858132, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24646448, Length=289, Percent_Identity=34.9480968858132, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24646452, Length=289, Percent_Identity=34.9480968858132, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24646450, Length=289, Percent_Identity=34.9480968858132, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI62472511, Length=289, Percent_Identity=34.9480968858132, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI28571528, Length=264, Percent_Identity=33.3333333333333, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24585516, Length=254, Percent_Identity=30.3149606299213, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI28574286, Length=283, Percent_Identity=30.3886925795053, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24585514, Length=281, Percent_Identity=28.8256227758007, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI28574282, Length=281, Percent_Identity=28.8256227758007, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI28574284, Length=281, Percent_Identity=28.8256227758007, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI45552429, Length=281, Percent_Identity=28.8256227758007, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI45551003, Length=281, Percent_Identity=28.8256227758007, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19921140, Length=271, Percent_Identity=26.1992619926199, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.29 [H]

Molecular weight: Translated: 34246; Mature: 34246

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVVLDGYTLNPGDNPWDEVASFGDLTLYDRTPAEMVVERSRGAQVLLTNKTVLSAATL
CEEEEEECEEECCCCCCHHHHHCCCCEEEECCCHHHHHHHHCCCCEEEEECCHHHHHHHH
AALPELRLVCVLATGYNVVDLEKSAQLGIPVVNVPEYGSDSVAQHAIALLLELTNRVAQY
HHCCCEEEEEEEECCCCEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
HQAVARGDWSASPDFTLVGEPLTELCGRSIGIVGLGRIGARVARIAQALGMEVLAYNPRH
HHHHHCCCCCCCCCEEEECCHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCCCEEEECCCC
RVAPEGISLRWLSLDQLFSQADVVSLHCPLNDENEGMVNQRTLSLMKPQALLINTSRGGL
CCCCCCCEEEEEEHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCE
VVERDLAEALNRGSIAGAAVDVAAREPIPADSPLLLAKNCIVTPHIAWATLAARRRLMAA
EEEHHHHHHHCCCCCCCCEEEHHCCCCCCCCCCEEEECCCEECCCHHHHHHHHHHHHHHH
TAANIASFLAGKPQNVVNGVQ
HHHHHHHHHCCCCHHHHCCCC
>Mature Secondary Structure
MKIVVLDGYTLNPGDNPWDEVASFGDLTLYDRTPAEMVVERSRGAQVLLTNKTVLSAATL
CEEEEEECEEECCCCCCHHHHHCCCCEEEECCCHHHHHHHHCCCCEEEEECCHHHHHHHH
AALPELRLVCVLATGYNVVDLEKSAQLGIPVVNVPEYGSDSVAQHAIALLLELTNRVAQY
HHCCCEEEEEEEECCCCEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
HQAVARGDWSASPDFTLVGEPLTELCGRSIGIVGLGRIGARVARIAQALGMEVLAYNPRH
HHHHHCCCCCCCCCEEEECCHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCCCEEEECCCC
RVAPEGISLRWLSLDQLFSQADVVSLHCPLNDENEGMVNQRTLSLMKPQALLINTSRGGL
CCCCCCCEEEEEEHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCE
VVERDLAEALNRGSIAGAAVDVAAREPIPADSPLLLAKNCIVTPHIAWATLAARRRLMAA
EEEHHHHHHHCCCCCCCCEEEHHCCCCCCCCCCEEEECCCEECCCHHHHHHHHHHHHHHH
TAANIASFLAGKPQNVVNGVQ
HHHHHHHHHCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144463; 1729225; 1657886 [H]