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The map label for this gene is miaA-2

Identifier: 197118032

GI number: 197118032

Start: 1910221

End: 1911141

Strand: Reverse

Name: miaA-2

Synonym: Gbem_1645

Alternate gene names: 197118032

Gene position: 1911141-1910221 (Counterclockwise)

Preceding gene: 197118033

Following gene: 197118031

Centisome position: 41.41

GC content: 61.78

Gene sequence:

>921_bases
TTGACGGACGAGACGGAGACACCCGAGCGCATCCCGAACCTGCTGGTAATTCTCGGTGCGACCGCCTCAGGCAAGACGAG
GCTCGGGGTGCAACTCGCCGGCAGGCTGAACGGCGAGATCATCTCCGCCGACTCGCGCCAGGTCTACTGCGGCATGGACA
TCGGCACGGGAAAAGATCTGCACGAGTACCAGGGGATACCCTACCACCTCATCGACGTGGCACCGGCGGGGCACGAATTC
AACCTCTTCCAGTTCCAGCGGCTTTTCCTCGCAGCCTTCACCGACATCTGCAGCAGGGGGGCCTTCCCGGTGCTGGTGGG
GGGAAGCGGCATGTACCTCGACTGCGTGCTGCGCGGCTACCGGCTCACCGAAGTGCCGCAGGACCCGGCTCTCAGGGAAG
AACTCGCCCCCCTCTCCATGGATGAACTCGCCTCCCGGCTGGTCCGCTCCAACCCAAGGCTTCACAACAGCACCGACCTC
ACCGAGCGCGCCCGCCTGATACGGGCGATAGAAATCGCGGAATACCGGGGCGAGGCAGGCGACGACTGGCCCGAGTTGAC
ACCTCTCACCATAGGGATACGGTGGGAGCGCGCGCAGTTGCGGGAGCGGATCACCAAAAGGCTCAAAGAGAGGATGGAAC
AAGGGATGGTCGAAGAGGTGGAGCGGCTGCACGCCATGGGGACCTCCTGGGAGCAGCTCGAATTCTACGGGCTCGAGTAC
CGTTACCTCGCGCGCTACCTGAAAGGGGAGCTTTCCAGAAACGACATGTTCCAGAAGTTGAACAGCGCCATCCACGACTT
CGCCAAGAAACAGGAGAACTGGTTCAGGAAGATGCAAACAAACGGCATCGCGATCAACTGGGTCGAGGGAAATAGCGACC
CGCTCGCGCAAGCGCTGGAACTGCTGGCAAAGAACAGATAA

Upstream 100 bases:

>100_bases
AGGAGTTGAAAGAGCAGGACCTCCTTAAAGCCGACAACCAACTGGCCCGGGCGCTCGACCTTCTGAAGGGGGTCAACCTG
CTGAAAGCGAGCGGCCGGCG

Downstream 100 bases:

>100_bases
AGATAGAGATTGAGATTGAGATTGAGATTGAGATTAAGGTAAAGATTTAAACCCATTCGGTTCCTTGGTTTTATCCCTTT
TATCCCCTTCATCCCTGTAA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase 2; DMAPP:tRNA dimethylallyltransferase 2; DMATase 2; Isopentenyl-diphosphate:tRNA isopentenyltransferase 2; IPP transferase 2; IPPT 2; IPTase 2

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MTDETETPERIPNLLVILGATASGKTRLGVQLAGRLNGEIISADSRQVYCGMDIGTGKDLHEYQGIPYHLIDVAPAGHEF
NLFQFQRLFLAAFTDICSRGAFPVLVGGSGMYLDCVLRGYRLTEVPQDPALREELAPLSMDELASRLVRSNPRLHNSTDL
TERARLIRAIEIAEYRGEAGDDWPELTPLTIGIRWERAQLRERITKRLKERMEQGMVEEVERLHAMGTSWEQLEFYGLEY
RYLARYLKGELSRNDMFQKLNSAIHDFAKKQENWFRKMQTNGIAINWVEGNSDPLAQALELLAKNR

Sequences:

>Translated_306_residues
MTDETETPERIPNLLVILGATASGKTRLGVQLAGRLNGEIISADSRQVYCGMDIGTGKDLHEYQGIPYHLIDVAPAGHEF
NLFQFQRLFLAAFTDICSRGAFPVLVGGSGMYLDCVLRGYRLTEVPQDPALREELAPLSMDELASRLVRSNPRLHNSTDL
TERARLIRAIEIAEYRGEAGDDWPELTPLTIGIRWERAQLRERITKRLKERMEQGMVEEVERLHAMGTSWEQLEFYGLEY
RYLARYLKGELSRNDMFQKLNSAIHDFAKKQENWFRKMQTNGIAINWVEGNSDPLAQALELLAKNR
>Mature_305_residues
TDETETPERIPNLLVILGATASGKTRLGVQLAGRLNGEIISADSRQVYCGMDIGTGKDLHEYQGIPYHLIDVAPAGHEFN
LFQFQRLFLAAFTDICSRGAFPVLVGGSGMYLDCVLRGYRLTEVPQDPALREELAPLSMDELASRLVRSNPRLHNSTDLT
ERARLIRAIEIAEYRGEAGDDWPELTPLTIGIRWERAQLRERITKRLKERMEQGMVEEVERLHAMGTSWEQLEFYGLEYR
YLARYLKGELSRNDMFQKLNSAIHDFAKKQENWFRKMQTNGIAINWVEGNSDPLAQALELLAKNR

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=311, Percent_Identity=25.7234726688103, Blast_Score=95, Evalue=9e-20,
Organism=Escherichia coli, GI1790613, Length=298, Percent_Identity=29.8657718120805, Blast_Score=114, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI25144712, Length=119, Percent_Identity=35.2941176470588, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324848, Length=288, Percent_Identity=26.3888888888889, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24649830, Length=243, Percent_Identity=30.8641975308642, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA2_GEOBB (B5E9C1)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002138459.1
- ProteinModelPortal:   B5E9C1
- SMR:   B5E9C1
- GeneID:   6781633
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_1645
- HOGENOM:   HBG559459
- OMA:   IYYGLEY
- ProtClustDB:   PRK00091
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34900; Mature: 34769

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDETETPERIPNLLVILGATASGKTRLGVQLAGRLNGEIISADSRQVYCGMDIGTGKDL
CCCCCCCHHHCCCEEEEEECCCCCCCEEEEEEECCCCCEEEECCCCEEEEECCCCCCCCH
HEYQGIPYHLIDVAPAGHEFNLFQFQRLFLAAFTDICSRGAFPVLVGGSGMYLDCVLRGY
HHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEHHHHCCC
RLTEVPQDPALREELAPLSMDELASRLVRSNPRLHNSTDLTERARLIRAIEIAEYRGEAG
EECCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
DDWPELTPLTIGIRWERAQLRERITKRLKERMEQGMVEEVERLHAMGTSWEQLEFYGLEY
CCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHH
RYLARYLKGELSRNDMFQKLNSAIHDFAKKQENWFRKMQTNGIAINWVEGNSDPLAQALE
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHH
LLAKNR
HHHCCC
>Mature Secondary Structure 
TDETETPERIPNLLVILGATASGKTRLGVQLAGRLNGEIISADSRQVYCGMDIGTGKDL
CCCCCCHHHCCCEEEEEECCCCCCCEEEEEEECCCCCEEEECCCCEEEEECCCCCCCCH
HEYQGIPYHLIDVAPAGHEFNLFQFQRLFLAAFTDICSRGAFPVLVGGSGMYLDCVLRGY
HHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEHHHHCCC
RLTEVPQDPALREELAPLSMDELASRLVRSNPRLHNSTDLTERARLIRAIEIAEYRGEAG
EECCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
DDWPELTPLTIGIRWERAQLRERITKRLKERMEQGMVEEVERLHAMGTSWEQLEFYGLEY
CCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHH
RYLARYLKGELSRNDMFQKLNSAIHDFAKKQENWFRKMQTNGIAINWVEGNSDPLAQALE
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHH
LLAKNR
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA