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The map label for this gene is capD [H]

Identifier: 197118020

GI number: 197118020

Start: 1896321

End: 1898264

Strand: Direct

Name: capD [H]

Synonym: Gbem_1633

Alternate gene names: 197118020

Gene position: 1896321-1898264 (Clockwise)

Preceding gene: 197118013

Following gene: 197118021

Centisome position: 41.09

GC content: 58.28

Gene sequence:

>1944_bases
ATGTTCCGTACAAGAAGGTTTCTGGTATTTTTCCTCGATGTTGCCCTGATTGTCGCCGCATTCACTCTCGCTTTCCTGCT
TCGATTCGATTTACAAATTCCTGCCGATCAGTCCAGTCCCCTGCTGCAAGGGCTTGTCATCGTTTTAGCTGTCAAATCGG
TCATCTTCATCGCCTCCGGCATGTACCGCTGCATCTGGCGTTACGCCTCTTTGCGGGACGCTTATGAAATCTTCAAGGTT
GTGACCCTGTCCTCCGTCGTCTCTGCTCTAGCGCTTCTTCTCATCAAAGGCCCTTTCGCTCTCCCGCGCTCCATCTACTT
TCTTGACTGGTTTCTCCTCTTCTCGCTGGTGGCTGGTAGCCGCCTGGTCTGGAGGGTTTACCGTGAGACGAGATTCTTTC
CTTCTTTGCACAAAGGAAAGAAAACACTGATCATCGGCGCCGGGGAGGCCGGCAACCTTCTGCTCAAAGAGATCCGCAAG
GAGGCCGATTCCCCCTATAACGTAATAGGATTCGTTGACGACGACATTGAGAAGCGCGGCATGCGTCTTTCCGGCGTTCG
GGTCCTTGGGGGCACCAACCGCCTCTGCGCCATGGTCAGAAAACACGCCATCGAAGAGGTAATCTTCGCCATCCCCTCCG
GGGACGGTGAACTGATGCGCCGGGTGATAGCCAACTGTAAGCGGAGCAAGGCGCGGTTCAAAACACTTCCTGGCATCACC
GCTATTGTCGACGGCAAGGTGTCGATGAGCCAGATCAAGGACGTGGAGATAGAGGATCTCCTGGGGCGCGACCCGGTGAT
GCTCGACCAGACGGCCATCCGCAACTACCTGACCGACAAGCGGGTCCTGGTGACCGGTGCGGCTGGAAGCATCGGTAGCG
AGATCTGCCGCCAGGTTGCGCGTTTCAAGCCGGCGAAGCTGGTCCTCTTCGATCACGCGGAGACCCCTCTTTTCTACATC
GAGAAGGAGCTTGCCGCTGCTTTCCCGGACCTGCGGATCATCCCCATGGTGGGGGACGTGAAAAACCAGGAGCGCGTGGA
GGCGGTATTCGACGAGTTCATTCCCGAGGTGGTCTTTCATGCCGCGGCCTACAAGCATGTCGCCATGATGGAGTACAACC
CGGCCGAGGCGGTGCTGAACAACGTCCTCGGGTCAAGAATCGTGGCGGATGCGGCGCACAAGACCAAGGTGAGCAACTTC
GTCATGGTCTCCACCGACAAGGCGGTGAACCCGACCAACGTCATGGGAGCCACCAAGCGTAGCGCCGAGATCTACGTGCA
GGCCCTGGCGGCGAAGAGCCAGACCAAGTTCACCACGGTCCGCTTCGGGAACGTCCTGGGCAGTAACGGCAGCGTGATAC
CGCTTTTCAAAGAGCAGATCAGAAAGGGAGGGCCGGTCACCGTCACCCACAGCGATGTGGTGCGCTACTTCATGACCATT
CCTGAGGCGTCGCAGCTGGTGATGCAGGCAGGCTGCATCGGGCGCGGCGGGGAGATCTTCGTGCTGGACATGGGAGAGCC
GGTCCGGATACTTTCTCTGGCCGAGGAGCTGATCAGGCTTTCCGGGCTGATCCCGTACAAGGAAATGGACATCCAGTTCA
CGGGGTTGAAGCCGGGCGAGAAGCTGTTCGAGGAGCTTTTGATCAACGGCGAGGGGATAAAGCCCACGAGCCACAAGAAG
ATACGGGTGATGGCGCCGGTGGAAACCGACCTGAGAAGGGTGAGCCAGGATCTGGACGAGCTGTTTGGGCATGCCAGAAA
CCAGGATATCAAGGAGCTTTTGAAGTCTTTGCGGCGTATGGTGCCCGAGTTCGTGCCGCGCTACGAGTTCGACGTGGCGC
CGCCGTTTGCCTTCCAGCGGGTCAGGCCGGACCTCTTCCCTCCGCAAAAGCTTACCCTGGTGAGGAACCTGCAGTTAGCC
TCAAAAGAGGGCTCCGCGGCTTGA

Upstream 100 bases:

>100_bases
TTTTGAGAGTAGGTGTTATAATCTCCATCTGCTCGGGCTAATGTATCAGGATTTAACATTTAGCTTGTCCAAACTTGATT
TCATGAGGGGTGGGGGGGGC

Downstream 100 bases:

>100_bases
AGACGCAAAGGTTAAATACTAAAGGCTAAAGCCGCAAACCTAAAACGACTCACGCAAAGCCGCAAAGACGCAAAGAAAGG
CTTCCTGTTCCCAACGGAGA

Product: NAD-dependent nucleoside diphosphate-sugar epimerase/dehydratase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
MFRTRRFLVFFLDVALIVAAFTLAFLLRFDLQIPADQSSPLLQGLVIVLAVKSVIFIASGMYRCIWRYASLRDAYEIFKV
VTLSSVVSALALLLIKGPFALPRSIYFLDWFLLFSLVAGSRLVWRVYRETRFFPSLHKGKKTLIIGAGEAGNLLLKEIRK
EADSPYNVIGFVDDDIEKRGMRLSGVRVLGGTNRLCAMVRKHAIEEVIFAIPSGDGELMRRVIANCKRSKARFKTLPGIT
AIVDGKVSMSQIKDVEIEDLLGRDPVMLDQTAIRNYLTDKRVLVTGAAGSIGSEICRQVARFKPAKLVLFDHAETPLFYI
EKELAAAFPDLRIIPMVGDVKNQERVEAVFDEFIPEVVFHAAAYKHVAMMEYNPAEAVLNNVLGSRIVADAAHKTKVSNF
VMVSTDKAVNPTNVMGATKRSAEIYVQALAAKSQTKFTTVRFGNVLGSNGSVIPLFKEQIRKGGPVTVTHSDVVRYFMTI
PEASQLVMQAGCIGRGGEIFVLDMGEPVRILSLAEELIRLSGLIPYKEMDIQFTGLKPGEKLFEELLINGEGIKPTSHKK
IRVMAPVETDLRRVSQDLDELFGHARNQDIKELLKSLRRMVPEFVPRYEFDVAPPFAFQRVRPDLFPPQKLTLVRNLQLA
SKEGSAA

Sequences:

>Translated_647_residues
MFRTRRFLVFFLDVALIVAAFTLAFLLRFDLQIPADQSSPLLQGLVIVLAVKSVIFIASGMYRCIWRYASLRDAYEIFKV
VTLSSVVSALALLLIKGPFALPRSIYFLDWFLLFSLVAGSRLVWRVYRETRFFPSLHKGKKTLIIGAGEAGNLLLKEIRK
EADSPYNVIGFVDDDIEKRGMRLSGVRVLGGTNRLCAMVRKHAIEEVIFAIPSGDGELMRRVIANCKRSKARFKTLPGIT
AIVDGKVSMSQIKDVEIEDLLGRDPVMLDQTAIRNYLTDKRVLVTGAAGSIGSEICRQVARFKPAKLVLFDHAETPLFYI
EKELAAAFPDLRIIPMVGDVKNQERVEAVFDEFIPEVVFHAAAYKHVAMMEYNPAEAVLNNVLGSRIVADAAHKTKVSNF
VMVSTDKAVNPTNVMGATKRSAEIYVQALAAKSQTKFTTVRFGNVLGSNGSVIPLFKEQIRKGGPVTVTHSDVVRYFMTI
PEASQLVMQAGCIGRGGEIFVLDMGEPVRILSLAEELIRLSGLIPYKEMDIQFTGLKPGEKLFEELLINGEGIKPTSHKK
IRVMAPVETDLRRVSQDLDELFGHARNQDIKELLKSLRRMVPEFVPRYEFDVAPPFAFQRVRPDLFPPQKLTLVRNLQLA
SKEGSAA
>Mature_647_residues
MFRTRRFLVFFLDVALIVAAFTLAFLLRFDLQIPADQSSPLLQGLVIVLAVKSVIFIASGMYRCIWRYASLRDAYEIFKV
VTLSSVVSALALLLIKGPFALPRSIYFLDWFLLFSLVAGSRLVWRVYRETRFFPSLHKGKKTLIIGAGEAGNLLLKEIRK
EADSPYNVIGFVDDDIEKRGMRLSGVRVLGGTNRLCAMVRKHAIEEVIFAIPSGDGELMRRVIANCKRSKARFKTLPGIT
AIVDGKVSMSQIKDVEIEDLLGRDPVMLDQTAIRNYLTDKRVLVTGAAGSIGSEICRQVARFKPAKLVLFDHAETPLFYI
EKELAAAFPDLRIIPMVGDVKNQERVEAVFDEFIPEVVFHAAAYKHVAMMEYNPAEAVLNNVLGSRIVADAAHKTKVSNF
VMVSTDKAVNPTNVMGATKRSAEIYVQALAAKSQTKFTTVRFGNVLGSNGSVIPLFKEQIRKGGPVTVTHSDVVRYFMTI
PEASQLVMQAGCIGRGGEIFVLDMGEPVRILSLAEELIRLSGLIPYKEMDIQFTGLKPGEKLFEELLINGEGIKPTSHKK
IRVMAPVETDLRRVSQDLDELFGHARNQDIKELLKSLRRMVPEFVPRYEFDVAPPFAFQRVRPDLFPPQKLTLVRNLQLA
SKEGSAA

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=267, Percent_Identity=25.4681647940075, Blast_Score=77, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 72213; Mature: 72213

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRTRRFLVFFLDVALIVAAFTLAFLLRFDLQIPADQSSPLLQGLVIVLAVKSVIFIASG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
MYRCIWRYASLRDAYEIFKVVTLSSVVSALALLLIKGPFALPRSIYFLDWFLLFSLVAGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
RLVWRVYRETRFFPSLHKGKKTLIIGAGEAGNLLLKEIRKEADSPYNVIGFVDDDIEKRG
HHHHHHHHHHHCCCCHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHCC
MRLSGVRVLGGTNRLCAMVRKHAIEEVIFAIPSGDGELMRRVIANCKRSKARFKTLPGIT
CEEECEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCE
AIVDGKVSMSQIKDVEIEDLLGRDPVMLDQTAIRNYLTDKRVLVTGAAGSIGSEICRQVA
EEECCCCCHHHHCCCCHHHHCCCCCCEECHHHHHHHHCCCEEEEEECCCCHHHHHHHHHH
RFKPAKLVLFDHAETPLFYIEKELAAAFPDLRIIPMVGDVKNQERVEAVFDEFIPEVVFH
HCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHH
AAAYKHVAMMEYNPAEAVLNNVLGSRIVADAAHKTKVSNFVMVSTDKAVNPTNVMGATKR
HHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHCCCCCH
SAEIYVQALAAKSQTKFTTVRFGNVLGSNGSVIPLFKEQIRKGGPVTVTHSDVVRYFMTI
HHHHHHHHHHHCCCCCEEEEEECCEECCCCCEEHHHHHHHHCCCCEEEEHHHHHHHHHHC
PEASQLVMQAGCIGRGGEIFVLDMGEPVRILSLAEELIRLSGLIPYKEMDIQFTGLKPGE
CHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHH
KLFEELLINGEGIKPTSHKKIRVMAPVETDLRRVSQDLDELFGHARNQDIKELLKSLRRM
HHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
VPEFVPRYEFDVAPPFAFQRVRPDLFPPQKLTLVRNLQLASKEGSAA
HHHHCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFRTRRFLVFFLDVALIVAAFTLAFLLRFDLQIPADQSSPLLQGLVIVLAVKSVIFIASG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
MYRCIWRYASLRDAYEIFKVVTLSSVVSALALLLIKGPFALPRSIYFLDWFLLFSLVAGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
RLVWRVYRETRFFPSLHKGKKTLIIGAGEAGNLLLKEIRKEADSPYNVIGFVDDDIEKRG
HHHHHHHHHHHCCCCHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHCC
MRLSGVRVLGGTNRLCAMVRKHAIEEVIFAIPSGDGELMRRVIANCKRSKARFKTLPGIT
CEEECEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCE
AIVDGKVSMSQIKDVEIEDLLGRDPVMLDQTAIRNYLTDKRVLVTGAAGSIGSEICRQVA
EEECCCCCHHHHCCCCHHHHCCCCCCEECHHHHHHHHCCCEEEEEECCCCHHHHHHHHHH
RFKPAKLVLFDHAETPLFYIEKELAAAFPDLRIIPMVGDVKNQERVEAVFDEFIPEVVFH
HCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHH
AAAYKHVAMMEYNPAEAVLNNVLGSRIVADAAHKTKVSNFVMVSTDKAVNPTNVMGATKR
HHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHCCCCCH
SAEIYVQALAAKSQTKFTTVRFGNVLGSNGSVIPLFKEQIRKGGPVTVTHSDVVRYFMTI
HHHHHHHHHHHCCCCCEEEEEECCEECCCCCEEHHHHHHHHCCCCEEEEHHHHHHHHHHC
PEASQLVMQAGCIGRGGEIFVLDMGEPVRILSLAEELIRLSGLIPYKEMDIQFTGLKPGE
CHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHH
KLFEELLINGEGIKPTSHKKIRVMAPVETDLRRVSQDLDELFGHARNQDIKELLKSLRRM
HHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
VPEFVPRYEFDVAPPFAFQRVRPDLFPPQKLTLVRNLQLASKEGSAA
HHHHCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]