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The map label for this gene is asnB-4 [H]

Identifier: 197118002

GI number: 197118002

Start: 1873762

End: 1875624

Strand: Direct

Name: asnB-4 [H]

Synonym: Gbem_1615

Alternate gene names: 197118002

Gene position: 1873762-1875624 (Clockwise)

Preceding gene: 197118001

Following gene: 197118003

Centisome position: 40.6

GC content: 62.69

Gene sequence:

>1863_bases
ATGTGTGGCATCTTTGGAGCACTATCGCCGCAGTCCGCCCCGGTGGAGCAACTGTACGAGAAGATTGCCAGCCTCGCCCA
CCGCGGTCCCGATGACTCAGGATGCTGGGTCTCACCGGACCGGACCGTCGTCCTCGGCCACCGTCGGCTCTCTATCCTGG
ACTTGTCTCAAGCTGGACACCAGCCCATGGCCAGTTCGTGCGGCCGCTACCACATAGTCTTTAACGGTGAGATCTACAAC
TACCTTGAGATCAGGGACGAACTGGCCGCACTGGGATTCCTTTTTTCTGGCAGCGGCGATACCGAGGTCGTCCTCGCCTC
CTACCGCCAGTGGGGCGCGGACTGTCTGGGGCGCTTTAATGGCATGTTCGCTCTGGCCATTTGGGATGAGGGAGATGTCG
CCAACCAGCCCCGTCTGTTCATGGCGCGAGACCGGGCGGGGAAGAAGCCGTTTTATTACGTCCACGATGGGCGTTCGTTA
CGCTTCGCTTCCGAATTGAAGGCGCTTGGCCCGGGAAAGAGTGTCGACCTGCGCGCGCTGAATTTCTACCTCGCCTTGGG
CTACGTACCCGACACGCTCTGCATCGCTGCCGGGGCTAAAAAGCTTCCCCCCGCCCATGCCGGCTACTTCCTCCCCGAAA
GCGGGGAGCTTGAGACCTGGCGCTATTGGAGCCTGCCCCAGAACCTCCCTGACCCGGGCCGCGGGTGCGAAGATCTCGCG
GACGAGGCGGAGAGCCTGCTGCGGGATTCGGTTGCCATGCGTCTGCGCAGCGACGTGCCCGTGGGGGTGTTGCTATCGGG
GGGGTTAGACTCGAGTCTGGTGGTGGCAGCCGCCGCTCAGGCCTCGACCACGCCGATCAAGACCTTCACCGTTTCGTTCC
CCGGCACCCGCTACGACGAGGCAGGATACGCCGCCATCGTGGCGCGCCACTTCGGGACCGAGCACCACGTGCTGGAGGTC
CCCCAGCCATCGTTGGGGACCCTAGACGCTTTCTCTCCCTTAATAGACGAGCCACTTGCCGACTCTTCGCTTCTGCCTGC
TTTCATGGTCTCCCGGTTAACCGTGCAGCACGTAAAGGTGGCGCTTGGTGGCGACGGCGGCGACGAACTTTTCGGCGGCT
ACGCCGACTACACCATGGCAGCTGCGGACCAGCGCTGTCTGGGCTGGATACCGAAGCCGCTGCTGTTGGCGGCGGGCCGT
GCGGCGGGAGCACTGCCCGCCGGGGTCAAGGGGCGCAACCGCCTCTTTGCCCTGCAGGGTGGGGCGTACGAGTCCATGAT
ATGGGGAAGCCCTTATTTCGACCTCTCGCTGCGCCGTCGCATCTTGGCCTCGGAGCAGGTTCAGGCCCTTGGTGAACAGT
TGGATGAGCCGGAGCGTTGGCTGATGTCGCTGTTCCGGACCGGCCGCGACCCTCTTGACTGCATGACCAGAACCCATTTC
GGAGGCATTTTGCCGGACGACTTCCTGGTGAAGGTGGATCGAGCGAGCATGGCGGTGAGCCTAGAGCTTCGCGCCCCGCT
CCTTGACTACCGGCTGGTCGAGTTCGCCTTCGGCAGGTTGCCTTCCCATTGGAAGGTACAGGGGCAGGAAAGTCGCCGGC
TCCAGAAGGTGCTAGGCCGTCGCATGCTCCCCCCCCAGCTCGACATCAACAGAAAACAGGGATTCTCCATACCTTTGGAC
AGCTGGCTGCGCAGTTCCCGGGGGGGCGAGGTCGAGGAAATGCGCCGCTGGTTACCGGACTGCATCGTACAGGACGAGGT
TGCGAGGCTCATCGCCGGGCATCTGAAAGGGCGTGCTAACGGCTCGCGCCTCTTCGCATTGATGATGCTTGCCTTGTCAC
AAAAAAATAGCCAGGAGAATTAG

Upstream 100 bases:

>100_bases
AAGCACCCGGAGCGCCTTCCCTACCTGAAGGAACGCTATGGCCTGAATACGCTGGATCTCTATGCCAACGGCAGCGTCAT
CATCTTGAGGAAATCCTGAC

Downstream 100 bases:

>100_bases
CTTTGACTAATAGACCACTGATAACCGTTTGTATTCCGGCATACAATCGCGCCCATGTTCTCGAAGCCCTGCTCGATTCA
ATCCTGTGTCAGTCTTTCGA

Product: asparagine synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 620; Mature: 620

Protein sequence:

>620_residues
MCGIFGALSPQSAPVEQLYEKIASLAHRGPDDSGCWVSPDRTVVLGHRRLSILDLSQAGHQPMASSCGRYHIVFNGEIYN
YLEIRDELAALGFLFSGSGDTEVVLASYRQWGADCLGRFNGMFALAIWDEGDVANQPRLFMARDRAGKKPFYYVHDGRSL
RFASELKALGPGKSVDLRALNFYLALGYVPDTLCIAAGAKKLPPAHAGYFLPESGELETWRYWSLPQNLPDPGRGCEDLA
DEAESLLRDSVAMRLRSDVPVGVLLSGGLDSSLVVAAAAQASTTPIKTFTVSFPGTRYDEAGYAAIVARHFGTEHHVLEV
PQPSLGTLDAFSPLIDEPLADSSLLPAFMVSRLTVQHVKVALGGDGGDELFGGYADYTMAAADQRCLGWIPKPLLLAAGR
AAGALPAGVKGRNRLFALQGGAYESMIWGSPYFDLSLRRRILASEQVQALGEQLDEPERWLMSLFRTGRDPLDCMTRTHF
GGILPDDFLVKVDRASMAVSLELRAPLLDYRLVEFAFGRLPSHWKVQGQESRRLQKVLGRRMLPPQLDINRKQGFSIPLD
SWLRSSRGGEVEEMRRWLPDCIVQDEVARLIAGHLKGRANGSRLFALMMLALSQKNSQEN

Sequences:

>Translated_620_residues
MCGIFGALSPQSAPVEQLYEKIASLAHRGPDDSGCWVSPDRTVVLGHRRLSILDLSQAGHQPMASSCGRYHIVFNGEIYN
YLEIRDELAALGFLFSGSGDTEVVLASYRQWGADCLGRFNGMFALAIWDEGDVANQPRLFMARDRAGKKPFYYVHDGRSL
RFASELKALGPGKSVDLRALNFYLALGYVPDTLCIAAGAKKLPPAHAGYFLPESGELETWRYWSLPQNLPDPGRGCEDLA
DEAESLLRDSVAMRLRSDVPVGVLLSGGLDSSLVVAAAAQASTTPIKTFTVSFPGTRYDEAGYAAIVARHFGTEHHVLEV
PQPSLGTLDAFSPLIDEPLADSSLLPAFMVSRLTVQHVKVALGGDGGDELFGGYADYTMAAADQRCLGWIPKPLLLAAGR
AAGALPAGVKGRNRLFALQGGAYESMIWGSPYFDLSLRRRILASEQVQALGEQLDEPERWLMSLFRTGRDPLDCMTRTHF
GGILPDDFLVKVDRASMAVSLELRAPLLDYRLVEFAFGRLPSHWKVQGQESRRLQKVLGRRMLPPQLDINRKQGFSIPLD
SWLRSSRGGEVEEMRRWLPDCIVQDEVARLIAGHLKGRANGSRLFALMMLALSQKNSQEN
>Mature_620_residues
MCGIFGALSPQSAPVEQLYEKIASLAHRGPDDSGCWVSPDRTVVLGHRRLSILDLSQAGHQPMASSCGRYHIVFNGEIYN
YLEIRDELAALGFLFSGSGDTEVVLASYRQWGADCLGRFNGMFALAIWDEGDVANQPRLFMARDRAGKKPFYYVHDGRSL
RFASELKALGPGKSVDLRALNFYLALGYVPDTLCIAAGAKKLPPAHAGYFLPESGELETWRYWSLPQNLPDPGRGCEDLA
DEAESLLRDSVAMRLRSDVPVGVLLSGGLDSSLVVAAAAQASTTPIKTFTVSFPGTRYDEAGYAAIVARHFGTEHHVLEV
PQPSLGTLDAFSPLIDEPLADSSLLPAFMVSRLTVQHVKVALGGDGGDELFGGYADYTMAAADQRCLGWIPKPLLLAAGR
AAGALPAGVKGRNRLFALQGGAYESMIWGSPYFDLSLRRRILASEQVQALGEQLDEPERWLMSLFRTGRDPLDCMTRTHF
GGILPDDFLVKVDRASMAVSLELRAPLLDYRLVEFAFGRLPSHWKVQGQESRRLQKVLGRRMLPPQLDINRKQGFSIPLD
SWLRSSRGGEVEEMRRWLPDCIVQDEVARLIAGHLKGRANGSRLFALMMLALSQKNSQEN

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786889, Length=404, Percent_Identity=31.9306930693069, Blast_Score=148, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71993933, Length=403, Percent_Identity=29.7766749379653, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI25147557, Length=403, Percent_Identity=29.7766749379653, Blast_Score=123, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI25147560, Length=358, Percent_Identity=28.7709497206704, Blast_Score=109, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6325403, Length=426, Percent_Identity=29.3427230046948, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6321563, Length=423, Percent_Identity=28.6052009456265, Blast_Score=114, Evalue=4e-26,
Organism=Drosophila melanogaster, GI45553209, Length=403, Percent_Identity=29.7766749379653, Blast_Score=131, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 68126; Mature: 68126

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIFGALSPQSAPVEQLYEKIASLAHRGPDDSGCWVSPDRTVVLGHRRLSILDLSQAGH
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCEEEEECCEEEEEEHHHCCC
QPMASSCGRYHIVFNGEIYNYLEIRDELAALGFLFSGSGDTEVVLASYRQWGADCLGRFN
CCHHHCCCCEEEEECCCEEHHHHHHHHHHHHHEEEECCCCCEEEEHHHHHHHHHHHHHCC
GMFALAIWDEGDVANQPRLFMARDRAGKKPFYYVHDGRSLRFASELKALGPGKSVDLRAL
CEEEEEEECCCCCCCCCCEEEEECCCCCCCEEEEECCCCEEHHHHHHHCCCCCCCCCEEE
NFYLALGYVPDTLCIAAGAKKLPPAHAGYFLPESGELETWRYWSLPQNLPDPGRGCEDLA
EEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHH
DEAESLLRDSVAMRLRSDVPVGVLLSGGLDSSLVVAAAAQASTTPIKTFTVSFPGTRYDE
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHEEEHHCCCCCCEEEEEEECCCCCCCC
AGYAAIVARHFGTEHHVLEVPQPSLGTLDAFSPLIDEPLADSSLLPAFMVSRLTVQHVKV
CCHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHEEE
ALGGDGGDELFGGYADYTMAAADQRCLGWIPKPLLLAAGRAAGALPAGVKGRNRLFALQG
EECCCCCHHHCCCHHHHHHHHHCCHHHHCCCCHHHHHCCCCCCCCCCCCCCCCEEEEEEC
GAYESMIWGSPYFDLSLRRRILASEQVQALGEQLDEPERWLMSLFRTGRDPLDCMTRTHF
CCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHC
GGILPDDFLVKVDRASMAVSLELRAPLLDYRLVEFAFGRLPSHWKVQGQESRRLQKVLGR
CCCCCCHHEEEEECCCEEEEEEECCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHH
RMLPPQLDINRKQGFSIPLDSWLRSSRGGEVEEMRRWLPDCIVQDEVARLIAGHLKGRAN
CCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GSRLFALMMLALSQKNSQEN
HHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MCGIFGALSPQSAPVEQLYEKIASLAHRGPDDSGCWVSPDRTVVLGHRRLSILDLSQAGH
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCEEEEECCEEEEEEHHHCCC
QPMASSCGRYHIVFNGEIYNYLEIRDELAALGFLFSGSGDTEVVLASYRQWGADCLGRFN
CCHHHCCCCEEEEECCCEEHHHHHHHHHHHHHEEEECCCCCEEEEHHHHHHHHHHHHHCC
GMFALAIWDEGDVANQPRLFMARDRAGKKPFYYVHDGRSLRFASELKALGPGKSVDLRAL
CEEEEEEECCCCCCCCCCEEEEECCCCCCCEEEEECCCCEEHHHHHHHCCCCCCCCCEEE
NFYLALGYVPDTLCIAAGAKKLPPAHAGYFLPESGELETWRYWSLPQNLPDPGRGCEDLA
EEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHH
DEAESLLRDSVAMRLRSDVPVGVLLSGGLDSSLVVAAAAQASTTPIKTFTVSFPGTRYDE
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHEEEHHCCCCCCEEEEEEECCCCCCCC
AGYAAIVARHFGTEHHVLEVPQPSLGTLDAFSPLIDEPLADSSLLPAFMVSRLTVQHVKV
CCHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHEEE
ALGGDGGDELFGGYADYTMAAADQRCLGWIPKPLLLAAGRAAGALPAGVKGRNRLFALQG
EECCCCCHHHCCCHHHHHHHHHCCHHHHCCCCHHHHHCCCCCCCCCCCCCCCCEEEEEEC
GAYESMIWGSPYFDLSLRRRILASEQVQALGEQLDEPERWLMSLFRTGRDPLDCMTRTHF
CCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHC
GGILPDDFLVKVDRASMAVSLELRAPLLDYRLVEFAFGRLPSHWKVQGQESRRLQKVLGR
CCCCCCHHEEEEECCCEEEEEEECCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHH
RMLPPQLDINRKQGFSIPLDSWLRSSRGGEVEEMRRWLPDCIVQDEVARLIAGHLKGRAN
CCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GSRLFALMMLALSQKNSQEN
HHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]