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The map label for this gene is selU [H]

Identifier: 197117912

GI number: 197117912

Start: 1765184

End: 1766224

Strand: Reverse

Name: selU [H]

Synonym: Gbem_1525

Alternate gene names: 197117912

Gene position: 1766224-1765184 (Counterclockwise)

Preceding gene: 197117952

Following gene: 197117910

Centisome position: 38.27

GC content: 62.73

Gene sequence:

>1041_bases
TTGGAAACAGTACCATTTAACGAAGAACTCATCGACACCCATCTCGTAGTCGACGTACGCACCCCCCTGGAATACGAGGA
GGATCACCTCCCCGGCGCCATCAACGTCCCGCTTCTCACCAACGAAGAGCGGGTGGAGATCGGCATCCTGCATAAAGAGA
CCGGCCCGCATGCCGCAAGGAGAAGGGGGCTCGAGCTCACCGCGCACCGCTTCCCGCAAATGGTGGAGGAGATAGCGACA
GCCGCGGCGGGAAGGCCGATCCTCGTCTACTGCTGGCGGGGAGGGCTCAGGAGCAAGACGGTCACCGTCATCCTGGATCT
CGCCGGCTTCAAGGCGGTGCAGCTGCAAGGAGGGTACAAGAGCTTCCGCCACGAGGTGAGCGAGTACTTCACCCCCTTCA
CCCCCAAGGCGCCGCTCGTCGTCTTGCACGGCATGACCGGCATCGGCAAGACCACGCTTTTGCAGCGCCTGAAAGAGCGC
GGCAACTCGGTGCTCGATCTGGAGGGGCTCGCCTGCCACCGCGGTTCCGCCTTCGGCCAGCTGGGATTGAACCAGACCCT
CACCCAGAAGCGCTTCGAGACCCTTTTGTGGGACGAGATCAGGAAGGCCCCGGCGGGGCGCCCACTCATCCTGGAAGGGG
AGAGCGAGCGCATCGGGCGGGTGTCGCTTCCCGGGGACTTCTACCAGAAGATGGCGGCCGGCATCAGGGTCTGGTGCCAC
GCGCAGCTCAAGACCCGGGTGCAGCGGCTCATCGACGAGTACGGTCTTCCCGGCTACAAGGAAGAGATGGCGGTAGCGCT
GCAGAGGATCCGGAAGAAACTGGGCGGGAAAAGGTGCGAGGAGCTGGCGGAAAACCTGGAGAAATGGGAGCTGGAGCCCT
TCATGGCGGGACTTGTGAACGACTACTACGACAAGGTCTACTACAAAAACCGCAGCTGGGAGGCGGACACGGAGCTTGGC
ATGGAGGATTTCGACCAGGCGGCCGTCGAGCTGGAGCGCTATCTATCCCTGCGTTTCAAAACGGATGGCGCGAGCGCCTG
A

Upstream 100 bases:

>100_bases
GTGCCGCCGGTGCCGGTTCCCTTTCCCTTCCCAAAAGCTCCTCCTTGCATTCCGCTGGGGCTCTCTTTATAATGAGCCCG
CCAAAAAGGAGCCCTGCAAC

Downstream 100 bases:

>100_bases
CCCGGTATTTCGCCAGCAGCTCCACCGGGTGGTAGGAGAGCTTCGCCTGCCTCAAGGCGGGCTCCCCCAGGTCCTGCTCC
CGATTCAGGTAGCTGCACTC

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
METVPFNEELIDTHLVVDVRTPLEYEEDHLPGAINVPLLTNEERVEIGILHKETGPHAARRRGLELTAHRFPQMVEEIAT
AAAGRPILVYCWRGGLRSKTVTVILDLAGFKAVQLQGGYKSFRHEVSEYFTPFTPKAPLVVLHGMTGIGKTTLLQRLKER
GNSVLDLEGLACHRGSAFGQLGLNQTLTQKRFETLLWDEIRKAPAGRPLILEGESERIGRVSLPGDFYQKMAAGIRVWCH
AQLKTRVQRLIDEYGLPGYKEEMAVALQRIRKKLGGKRCEELAENLEKWELEPFMAGLVNDYYDKVYYKNRSWEADTELG
MEDFDQAAVELERYLSLRFKTDGASA

Sequences:

>Translated_346_residues
METVPFNEELIDTHLVVDVRTPLEYEEDHLPGAINVPLLTNEERVEIGILHKETGPHAARRRGLELTAHRFPQMVEEIAT
AAAGRPILVYCWRGGLRSKTVTVILDLAGFKAVQLQGGYKSFRHEVSEYFTPFTPKAPLVVLHGMTGIGKTTLLQRLKER
GNSVLDLEGLACHRGSAFGQLGLNQTLTQKRFETLLWDEIRKAPAGRPLILEGESERIGRVSLPGDFYQKMAAGIRVWCH
AQLKTRVQRLIDEYGLPGYKEEMAVALQRIRKKLGGKRCEELAENLEKWELEPFMAGLVNDYYDKVYYKNRSWEADTELG
MEDFDQAAVELERYLSLRFKTDGASA
>Mature_346_residues
METVPFNEELIDTHLVVDVRTPLEYEEDHLPGAINVPLLTNEERVEIGILHKETGPHAARRRGLELTAHRFPQMVEEIAT
AAAGRPILVYCWRGGLRSKTVTVILDLAGFKAVQLQGGYKSFRHEVSEYFTPFTPKAPLVVLHGMTGIGKTTLLQRLKER
GNSVLDLEGLACHRGSAFGQLGLNQTLTQKRFETLLWDEIRKAPAGRPLILEGESERIGRVSLPGDFYQKMAAGIRVWCH
AQLKTRVQRLIDEYGLPGYKEEMAVALQRIRKKLGGKRCEELAENLEKWELEPFMAGLVNDYYDKVYYKNRSWEADTELG
MEDFDQAAVELERYLSLRFKTDGASA

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA [H]

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1786712, Length=330, Percent_Identity=33.9393939393939, Blast_Score=141, Evalue=8e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR017582 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 39213; Mature: 39213

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00380 RHODANESE_1 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METVPFNEELIDTHLVVDVRTPLEYEEDHLPGAINVPLLTNEERVEIGILHKETGPHAAR
CCCCCCCHHHHCEEEEEEECCCCCCCCCCCCCEEECCEECCCCCEEEEEEECCCCCHHHH
RRGLELTAHRFPQMVEEIATAAAGRPILVYCWRGGLRSKTVTVILDLAGFKAVQLQGGYK
HCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEECCCCEEEEECCCHH
SFRHEVSEYFTPFTPKAPLVVLHGMTGIGKTTLLQRLKERGNSVLDLEGLACHRGSAFGQ
HHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHC
LGLNQTLTQKRFETLLWDEIRKAPAGRPLILEGESERIGRVSLPGDFYQKMAAGIRVWCH
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEECCCHHHHHHHHHHHHHEEH
AQLKTRVQRLIDEYGLPGYKEEMAVALQRIRKKLGGKRCEELAENLEKWELEPFMAGLVN
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHH
DYYDKVYYKNRSWEADTELGMEDFDQAAVELERYLSLRFKTDGASA
HHHHHHEECCCCCCCHHHCCHHHHHHHHHHHHHHHHEEEECCCCCC
>Mature Secondary Structure
METVPFNEELIDTHLVVDVRTPLEYEEDHLPGAINVPLLTNEERVEIGILHKETGPHAAR
CCCCCCCHHHHCEEEEEEECCCCCCCCCCCCCEEECCEECCCCCEEEEEEECCCCCHHHH
RRGLELTAHRFPQMVEEIATAAAGRPILVYCWRGGLRSKTVTVILDLAGFKAVQLQGGYK
HCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEECCCCEEEEECCCHH
SFRHEVSEYFTPFTPKAPLVVLHGMTGIGKTTLLQRLKERGNSVLDLEGLACHRGSAFGQ
HHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHC
LGLNQTLTQKRFETLLWDEIRKAPAGRPLILEGESERIGRVSLPGDFYQKMAAGIRVWCH
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEECCCHHHHHHHHHHHHHEEH
AQLKTRVQRLIDEYGLPGYKEEMAVALQRIRKKLGGKRCEELAENLEKWELEPFMAGLVN
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHH
DYYDKVYYKNRSWEADTELGMEDFDQAAVELERYLSLRFKTDGASA
HHHHHHEECCCCCCCHHHCCHHHHHHHHHHHHHHHHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA