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The map label for this gene is apl-2 [H]

Identifier: 197117901

GI number: 197117901

Start: 1752242

End: 1753762

Strand: Reverse

Name: apl-2 [H]

Synonym: Gbem_1514

Alternate gene names: 197117901

Gene position: 1753762-1752242 (Counterclockwise)

Preceding gene: 197117902

Following gene: 197117900

Centisome position: 38.0

GC content: 65.55

Gene sequence:

>1521_bases
ATGAATGATTCCTCCTGGTCCATCTTCGTGGTGCTTTTGGTGCTGGCTTCCTTCATCGTGGTGGGGCTCAGGCAGAAGTC
GCGCGAGAGTCAGGAGTACGGCTTCGGCGGCCGCTACACCGGGAGGATCGGGGGAGGTGCGGCCATCGCCAGCAACTGGA
TGAGCGCCGCAAGCCTCATGGGTCTTGCCGGGATCATCTACCTGCAGGGGTACCAGGGGCTCGCCTACGTGATCGGCTGG
ACCGGGGGGTACGTGCTGCTCCTCGTGCTCCTGGCGAGCCAGATCCGCCGCTTCGGCAAGTTCACCGCCCCCGAGTTCGT
GGGGGAGCGCTACGGCTCCCAGGCCGCGCGCGCCATCGCCGCCGCCATCTCCATTGCCATCTCCATCATCTACTGCGTGG
CCCAGTTCAAGGGGATAGGCCTCATCTTCTCCTTCATGTTCGGCATCGACTACCAGCAGGGGGTGACCTACGGGGCGCTG
GCCGTGGTCTCCTACCTGGTCATCTCGGGGATGCTGGGGGTCCCGAGAAACCAGCAGCTGCAATACCTGGTGATCAGCGT
CTCCTTCATCGTCCCGCTCATGTGGCTCGCCCGAAAGCTCGGCTACTTCTGGCTCCTCCCCCAGTTCGGCTACGGCCGCG
CTCTTACCGATCTGTCGCGCCAGTTCAACATCGACTTCACCCTCCCCTTCGCCAACGGCTCCCTCTACCAGTGGTGCGCG
CTCTGTTTCACACTCATGGTCGGCACCGCGGGGCTTCCCCACGTGCTGTCGCGCTTCTACACGGTACCCAACGTGCGCGA
CGCGCGCTGGAGCGTGGTCTGGGGGCTCTTCTTCATCGCCCTCATCTACTGGTCCGCCCCCGCCTTCTCCGTCTTCGGCA
GGCTCTTGGAGGCGAGAAGCGGCCTCATCCCCGACCCCGCCGCGGCCCGCGATGCAGCCGACGTGATCGCGCTTAAGACT
GCGGTCTGGGCGGGGCTTCCGGGGTGGCTCGTCGGGGTTCTCGCCGCAGGGGCCCTCTCGGCCGCCTTCTTCACCGTGGC
GGGGCTGCTGATGACCGGCGCCGCCTCGATCTCCCACGATATCTACTATTCCATGGTCAACCGCAGCGCCAGCGAATCAG
CGCGGATGCAGGTGGCCAAGGGTGGGACCTTGGTGCTCGCCGCCATCGTGCTCCTTTTGGCGCTCGACCCGCCGGGTCTG
ATCGCGGAGATCACGGCCGTCGCCTTCGCTTTGGCAGGCAACACCATCTTCCCGCTCTTTCTTCTGGGGATCTGGTGGGG
GCGCGCCAACCGCTACGGCGCCATAGCGGGGATGCTGACCGGCATCGTCTGTACCGCCATCGCCCCTCTTTGCGGCGGTA
TTTTCCCGCAGCTGGCCCTCCTCTTCCCGGTCACCTCGTCGGCGCTTCTGGGGGCCCCCCTGGTGATAGCGGTGATGATA
GCGGTTTCGCTCGCCACCCCCGCCCCATCGGAGGAGATGGGGCGCTTTCTGGAAAAAGAGGTGCACGGGCACCTCGACTG
A

Upstream 100 bases:

>100_bases
GGGCAGTTCCTGCCGCTTTGGTTCATCGTCCTTTGCGTGGTCTTCAACCTGTACGTGGACCGCATCACCGAATACCACAG
CCGCAAGCGGGACAAAAGCT

Downstream 100 bases:

>100_bases
CAACGTCTTTGTACTGGGAGCCGTAAATGGCGATGCTGCTGGGAACCAAAGGAGACGACATCCTCAAGTGGCGCGAGAGC
GCCCGGCTGGTGGAGGGGCT

Product: sodium/solute symporter family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MNDSSWSIFVVLLVLASFIVVGLRQKSRESQEYGFGGRYTGRIGGGAAIASNWMSAASLMGLAGIIYLQGYQGLAYVIGW
TGGYVLLLVLLASQIRRFGKFTAPEFVGERYGSQAARAIAAAISIAISIIYCVAQFKGIGLIFSFMFGIDYQQGVTYGAL
AVVSYLVISGMLGVPRNQQLQYLVISVSFIVPLMWLARKLGYFWLLPQFGYGRALTDLSRQFNIDFTLPFANGSLYQWCA
LCFTLMVGTAGLPHVLSRFYTVPNVRDARWSVVWGLFFIALIYWSAPAFSVFGRLLEARSGLIPDPAAARDAADVIALKT
AVWAGLPGWLVGVLAAGALSAAFFTVAGLLMTGAASISHDIYYSMVNRSASESARMQVAKGGTLVLAAIVLLLALDPPGL
IAEITAVAFALAGNTIFPLFLLGIWWGRANRYGAIAGMLTGIVCTAIAPLCGGIFPQLALLFPVTSSALLGAPLVIAVMI
AVSLATPAPSEEMGRFLEKEVHGHLD

Sequences:

>Translated_506_residues
MNDSSWSIFVVLLVLASFIVVGLRQKSRESQEYGFGGRYTGRIGGGAAIASNWMSAASLMGLAGIIYLQGYQGLAYVIGW
TGGYVLLLVLLASQIRRFGKFTAPEFVGERYGSQAARAIAAAISIAISIIYCVAQFKGIGLIFSFMFGIDYQQGVTYGAL
AVVSYLVISGMLGVPRNQQLQYLVISVSFIVPLMWLARKLGYFWLLPQFGYGRALTDLSRQFNIDFTLPFANGSLYQWCA
LCFTLMVGTAGLPHVLSRFYTVPNVRDARWSVVWGLFFIALIYWSAPAFSVFGRLLEARSGLIPDPAAARDAADVIALKT
AVWAGLPGWLVGVLAAGALSAAFFTVAGLLMTGAASISHDIYYSMVNRSASESARMQVAKGGTLVLAAIVLLLALDPPGL
IAEITAVAFALAGNTIFPLFLLGIWWGRANRYGAIAGMLTGIVCTAIAPLCGGIFPQLALLFPVTSSALLGAPLVIAVMI
AVSLATPAPSEEMGRFLEKEVHGHLD
>Mature_506_residues
MNDSSWSIFVVLLVLASFIVVGLRQKSRESQEYGFGGRYTGRIGGGAAIASNWMSAASLMGLAGIIYLQGYQGLAYVIGW
TGGYVLLLVLLASQIRRFGKFTAPEFVGERYGSQAARAIAAAISIAISIIYCVAQFKGIGLIFSFMFGIDYQQGVTYGAL
AVVSYLVISGMLGVPRNQQLQYLVISVSFIVPLMWLARKLGYFWLLPQFGYGRALTDLSRQFNIDFTLPFANGSLYQWCA
LCFTLMVGTAGLPHVLSRFYTVPNVRDARWSVVWGLFFIALIYWSAPAFSVFGRLLEARSGLIPDPAAARDAADVIALKT
AVWAGLPGWLVGVLAAGALSAAFFTVAGLLMTGAASISHDIYYSMVNRSASESARMQVAKGGTLVLAAIVLLLALDPPGL
IAEITAVAFALAGNTIFPLFLLGIWWGRANRYGAIAGMLTGIVCTAIAPLCGGIFPQLALLFPVTSSALLGAPLVIAVMI
AVSLATPAPSEEMGRFLEKEVHGHLD

Specific function: Unknown

COG id: COG4147

COG function: function code R; Predicted symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=516, Percent_Identity=23.8372093023256, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1790503, Length=478, Percent_Identity=28.2426778242678, Blast_Score=158, Evalue=9e-40,
Organism=Escherichia coli, GI1787251, Length=524, Percent_Identity=25.1908396946565, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI87082237, Length=461, Percent_Identity=23.8611713665944, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 54085; Mature: 54085

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS00457 NA_SOLUT_SYMP_2 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDSSWSIFVVLLVLASFIVVGLRQKSRESQEYGFGGRYTGRIGGGAAIASNWMSAASLM
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
GLAGIIYLQGYQGLAYVIGWTGGYVLLLVLLASQIRRFGKFTAPEFVGERYGSQAARAIA
HHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
AAISIAISIIYCVAQFKGIGLIFSFMFGIDYQQGVTYGALAVVSYLVISGMLGVPRNQQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCCH
QYLVISVSFIVPLMWLARKLGYFWLLPQFGYGRALTDLSRQFNIDFTLPFANGSLYQWCA
HHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCHHHHHH
LCFTLMVGTAGLPHVLSRFYTVPNVRDARWSVVWGLFFIALIYWSAPAFSVFGRLLEARS
HHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
GLIPDPAAARDAADVIALKTAVWAGLPGWLVGVLAAGALSAAFFTVAGLLMTGAASISHD
CCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYYSMVNRSASESARMQVAKGGTLVLAAIVLLLALDPPGLIAEITAVAFALAGNTIFPLF
HHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHH
LLGIWWGRANRYGAIAGMLTGIVCTAIAPLCGGIFPQLALLFPVTSSALLGAPLVIAVMI
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVSLATPAPSEEMGRFLEKEVHGHLD
HHHHCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNDSSWSIFVVLLVLASFIVVGLRQKSRESQEYGFGGRYTGRIGGGAAIASNWMSAASLM
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
GLAGIIYLQGYQGLAYVIGWTGGYVLLLVLLASQIRRFGKFTAPEFVGERYGSQAARAIA
HHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
AAISIAISIIYCVAQFKGIGLIFSFMFGIDYQQGVTYGALAVVSYLVISGMLGVPRNQQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCCH
QYLVISVSFIVPLMWLARKLGYFWLLPQFGYGRALTDLSRQFNIDFTLPFANGSLYQWCA
HHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCHHHHHH
LCFTLMVGTAGLPHVLSRFYTVPNVRDARWSVVWGLFFIALIYWSAPAFSVFGRLLEARS
HHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
GLIPDPAAARDAADVIALKTAVWAGLPGWLVGVLAAGALSAAFFTVAGLLMTGAASISHD
CCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYYSMVNRSASESARMQVAKGGTLVLAAIVLLLALDPPGLIAEITAVAFALAGNTIFPLF
HHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHH
LLGIWWGRANRYGAIAGMLTGIVCTAIAPLCGGIFPQLALLFPVTSSALLGAPLVIAVMI
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVSLATPAPSEEMGRFLEKEVHGHLD
HHHHCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]