Click here to switch to the map view.

The map label for this gene is oprF [H]

Identifier: 197117764

GI number: 197117764

Start: 1590785

End: 1592191

Strand: Direct

Name: oprF [H]

Synonym: Gbem_1376

Alternate gene names: 197117764

Gene position: 1590785-1592191 (Clockwise)

Preceding gene: 197117763

Following gene: 197117765

Centisome position: 34.47

GC content: 61.19

Gene sequence:

>1407_bases
ATGAAAAAACTCACCAGCATTATCTGTCTAATGGCCTTGGCCGTGATGGCGCTAGCCAGCATCGCGCAGGCCGGGGAAAG
AGCGGGGGCGTTTTCCATCTCCCCCTTTGTCGGCGGCTACGGGTTCGATGACGACCGCCAAGCCTATTTCAAGCAGAACC
GCCCGTTAGGCGGCTTGCGATTGGGTTACGACTTCACCGATCACTTCGCGGCGGAACTGGTCGGTACTTACATGAAGGCT
GAGTACAAGGCAACCAAACACAACGTCGGCGTCTACTCGTACCGCCTCGACCTCGTCTACAACATCCTCGCTGACTCTCA
GGTCGTTCCCTATCTAGCATTCGGCGGCGGTGGGACCACCATCAGGGACGAACGCGGCAACTCCCATGATGGCACCGCCG
AAGCGGGCGGCGGCGTCAAGTTCTTCATGACCGACAACGTGGCCCTCAGGGCAGACGGGCGCTATCTCGTCGATTTCATG
TCCAACCACCTGAGTCGCGGCCGTGACCTTGCCCGTAACTGGGAATACTCCCTCGGCTTGACCTTCCTGTTCGGCGGCAG
GGAGCGCGCTGCGGCTCCCGCCGAGACCGTTGCCACTCCAGTCGAGCAGCCCGCTGCGGTTACCCCTGCCCCCGAGCCCC
CGCTTGAGGTTCCGGCAGCCGAGCCGACCCCTGATCACTACAAGTATTGCATCACCCTGCATGGTGAGTTCGACATCGAC
AAGGCCATCATCCGCCCTGAATACAAGGAGGAGATCGCCCAGGTCGGCAACTTCATGAAAAAGTACCCGACCACCACGGC
CGTCATCGAAGGGCACACCGATAACGTCGGCTCCTACAATCACAACCAGGATCTCTCCCAGCGCCGCGCCGAGGCGGTGG
TGAACTACCTGGTCGAGCAGTACGGGATCGAGCGTTCCCGTCTTTCCGCCAAGGGGTACGGTTTCACCCGCCCCATCGCC
GACAACGCGACCGACGAGGGTAAACAGAAAAACCGCCGCATCGAGGCGATCATCGACTGCGCCTTCGACGTGCAGCAGGT
TACTCCGCCGGACAGGCTCTGCATGGCGCTGGTGCTGGATTTCGATGCCGGCAAGGCGGACGTGAAGTCGCAGTACCGCA
ACGAGATCGCCAAGGTGGCTGACTACATGAAGCGCTACAACACCACCACCGCCGTGATCGAGGGGCATACCGACAACACC
GGCTCCCCCGAAAAGAACATGAAGCTCTCGCAGCAGCGCGCCCAGAACGTGGTCAACTACCTGGTGGACAACTTCGGCAT
CGAGCGCTCCCGCCTTTCCGCCAAGGGGTACGGCGCGACCCGCCGCATCAGCTACAACAACACGCCCGAAGGGCGCCAGA
AAAACCGCAGGATCAACGCGGTCATCGACTGCGTCGTCACGAAGTAG

Upstream 100 bases:

>100_bases
GGTCCGGCAGGCCGGAAACCGAAACCGGGTCCGCCATAATGGCGGGACTCCAATTTACCGGAAGGATCAGGAGATCTTTC
CGAACCAAGGAGGAAACAGG

Downstream 100 bases:

>100_bases
CCGCAGACCAGGTGCAACCGGGTAGTCGGCAGCACCCTGGCCTCTGGCAACGGTAACCACGGTGCCACGAAAATAGCCCA
TAGACGATATGGGCTATTTT

Product: OmpA family outer membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MKKLTSIICLMALAVMALASIAQAGERAGAFSISPFVGGYGFDDDRQAYFKQNRPLGGLRLGYDFTDHFAAELVGTYMKA
EYKATKHNVGVYSYRLDLVYNILADSQVVPYLAFGGGGTTIRDERGNSHDGTAEAGGGVKFFMTDNVALRADGRYLVDFM
SNHLSRGRDLARNWEYSLGLTFLFGGRERAAAPAETVATPVEQPAAVTPAPEPPLEVPAAEPTPDHYKYCITLHGEFDID
KAIIRPEYKEEIAQVGNFMKKYPTTTAVIEGHTDNVGSYNHNQDLSQRRAEAVVNYLVEQYGIERSRLSAKGYGFTRPIA
DNATDEGKQKNRRIEAIIDCAFDVQQVTPPDRLCMALVLDFDAGKADVKSQYRNEIAKVADYMKRYNTTTAVIEGHTDNT
GSPEKNMKLSQQRAQNVVNYLVDNFGIERSRLSAKGYGATRRISYNNTPEGRQKNRRINAVIDCVVTK

Sequences:

>Translated_468_residues
MKKLTSIICLMALAVMALASIAQAGERAGAFSISPFVGGYGFDDDRQAYFKQNRPLGGLRLGYDFTDHFAAELVGTYMKA
EYKATKHNVGVYSYRLDLVYNILADSQVVPYLAFGGGGTTIRDERGNSHDGTAEAGGGVKFFMTDNVALRADGRYLVDFM
SNHLSRGRDLARNWEYSLGLTFLFGGRERAAAPAETVATPVEQPAAVTPAPEPPLEVPAAEPTPDHYKYCITLHGEFDID
KAIIRPEYKEEIAQVGNFMKKYPTTTAVIEGHTDNVGSYNHNQDLSQRRAEAVVNYLVEQYGIERSRLSAKGYGFTRPIA
DNATDEGKQKNRRIEAIIDCAFDVQQVTPPDRLCMALVLDFDAGKADVKSQYRNEIAKVADYMKRYNTTTAVIEGHTDNT
GSPEKNMKLSQQRAQNVVNYLVDNFGIERSRLSAKGYGATRRISYNNTPEGRQKNRRINAVIDCVVTK
>Mature_468_residues
MKKLTSIICLMALAVMALASIAQAGERAGAFSISPFVGGYGFDDDRQAYFKQNRPLGGLRLGYDFTDHFAAELVGTYMKA
EYKATKHNVGVYSYRLDLVYNILADSQVVPYLAFGGGGTTIRDERGNSHDGTAEAGGGVKFFMTDNVALRADGRYLVDFM
SNHLSRGRDLARNWEYSLGLTFLFGGRERAAAPAETVATPVEQPAAVTPAPEPPLEVPAAEPTPDHYKYCITLHGEFDID
KAIIRPEYKEEIAQVGNFMKKYPTTTAVIEGHTDNVGSYNHNQDLSQRRAEAVVNYLVEQYGIERSRLSAKGYGFTRPIA
DNATDEGKQKNRRIEAIIDCAFDVQQVTPPDRLCMALVLDFDAGKADVKSQYRNEIAKVADYMKRYNTTTAVIEGHTDNT
GSPEKNMKLSQQRAQNVVNYLVDNFGIERSRLSAKGYGATRRISYNNTPEGRQKNRRINAVIDCVVTK

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=99, Percent_Identity=39.3939393939394, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787191, Length=365, Percent_Identity=27.6712328767123, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788957, Length=95, Percent_Identity=34.7368421052632, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 51859; Mature: 51859

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLTSIICLMALAVMALASIAQAGERAGAFSISPFVGGYGFDDDRQAYFKQNRPLGGLR
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHCCCCCCCEE
LGYDFTDHFAAELVGTYMKAEYKATKHNVGVYSYRLDLVYNILADSQVVPYLAFGGGGTT
ECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCEEEECCCCCE
IRDERGNSHDGTAEAGGGVKFFMTDNVALRADGRYLVDFMSNHLSRGRDLARNWEYSLGL
EECCCCCCCCCCCCCCCCEEEEEECCEEEEECCCHHHHHHHHHHHHCHHHHHCCCCEEEE
TFLFGGRERAAAPAETVATPVEQPAAVTPAPEPPLEVPAAEPTPDHYKYCITLHGEFDID
EEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCC
KAIIRPEYKEEIAQVGNFMKKYPTTTAVIEGHTDNVGSYNHNQDLSQRRAEAVVNYLVEQ
HHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
YGIERSRLSAKGYGFTRPIADNATDEGKQKNRRIEAIIDCAFDVQQVTPPDRLCMALVLD
HCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHEE
FDAGKADVKSQYRNEIAKVADYMKRYNTTTAVIEGHTDNTGSPEKNMKLSQQRAQNVVNY
CCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
LVDNFGIERSRLSAKGYGATRRISYNNTPEGRQKNRRINAVIDCVVTK
HHHHCCCCHHHCCCCCCCCEEEECCCCCCCCHHHCCCEEEEEEEEECC
>Mature Secondary Structure
MKKLTSIICLMALAVMALASIAQAGERAGAFSISPFVGGYGFDDDRQAYFKQNRPLGGLR
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHCCCCCCCEE
LGYDFTDHFAAELVGTYMKAEYKATKHNVGVYSYRLDLVYNILADSQVVPYLAFGGGGTT
ECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCEEEECCCCCE
IRDERGNSHDGTAEAGGGVKFFMTDNVALRADGRYLVDFMSNHLSRGRDLARNWEYSLGL
EECCCCCCCCCCCCCCCCEEEEEECCEEEEECCCHHHHHHHHHHHHCHHHHHCCCCEEEE
TFLFGGRERAAAPAETVATPVEQPAAVTPAPEPPLEVPAAEPTPDHYKYCITLHGEFDID
EEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCC
KAIIRPEYKEEIAQVGNFMKKYPTTTAVIEGHTDNVGSYNHNQDLSQRRAEAVVNYLVEQ
HHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
YGIERSRLSAKGYGFTRPIADNATDEGKQKNRRIEAIIDCAFDVQQVTPPDRLCMALVLD
HCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHEE
FDAGKADVKSQYRNEIAKVADYMKRYNTTTAVIEGHTDNTGSPEKNMKLSQQRAQNVVNY
CCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
LVDNFGIERSRLSAKGYGATRRISYNNTPEGRQKNRRINAVIDCVVTK
HHHHCCCCHHHCCCCCCCCEEEECCCCCCCCHHHCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1898935 [H]