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The map label for this gene is moaA-1 [H]

Identifier: 197117742

GI number: 197117742

Start: 1561424

End: 1562404

Strand: Direct

Name: moaA-1 [H]

Synonym: Gbem_1354

Alternate gene names: 197117742

Gene position: 1561424-1562404 (Clockwise)

Preceding gene: 197117735

Following gene: 197117743

Centisome position: 33.83

GC content: 63.0

Gene sequence:

>981_bases
ATGGCACTGATCGATGCCTACGGCAGGCGCATAAACTATTTGAGACTCTCGGTCACCGACCGCTGCAACATGCGCTGCAG
CTACTGCATGCCGGCGCAAGGCGTGGAGAAGCTCGAGCATAAAGAGATGCTGAGCTACGAAGAGCTCTACCGGGTAGCCG
GCGCCTGCATCGCACAGGGGATAGAGAAGATCCGGGTGACCGGCGGGGAACCGCTGGTGAGGAAGGGAATCGTGCCTTTC
TTGGAGCGTCTGGCGAGGGTGCCTGGCTTGAAGGAGCTGGTGCTGACCACCAACGGACTGCAACTGGAGGAGATGGCCCA
GCCCTTAAAGCGCGCCGGGGTGGCGCGGCTCAACATCAGCCTCGATTCGCTCAGGCCCGAGGTCTTCGCCCGCATCACCC
GTGGGGCGGACCTGAAGCGGGTGCTCTCCGGGATAGAGGCAGCAGAAAAGGCGGGTTTCGCGAACCTCAAAATAAACATG
GTAGTGATGCGCGGGGTTAACGACGACGAGATCCTGGACTTCGCCGCCCTCAGCATCGAAAAGCCGTACACGGTGCGCTT
CATAGAGTACATGCCGACCCTGCAGGACGAGGGGTGGAACGCGCAAAGCATGCCGGGGAGCGAGATCCTCGCGCGGATCG
CCGAGCGCTATCCGCTCCTCCCCATGGTGGGTGCAGAGATGGCGGGACCGGCCCGCAACTTCAAGATAGAGGGCGCGGCC
GGGGCCATCGGGATCATCACCCCGGTTTCCGGGCACTTCTGCGAGAGCTGCAACAGGATCCGGGTCACCGCCGCCGGCAG
GATGCGCGGCTGCCTCTTCTCCGACCAGGGTGTCGATCTCAAACCGCTGCTGCAGAGCCTCGATCCCTACCGGCTGCAGG
AAGAGATCCGGCGCATCGTGACGCACAAGCCGGGGCGGCATCACATTGCCGAGCCGGAAGCGGAGAAGATAACGGTCAAC
ATGTCCCGCATCGGTGGGTGA

Upstream 100 bases:

>100_bases
TTGTCCGACGTTTTGTCGCAGTTGGTATCGATATTGCCTTTGTATACAAACAGGCAGTACACGAGGCGGATCCCTTTATT
AAGAATTTGGGAGTTGCGGC

Downstream 100 bases:

>100_bases
AGCAGGGGCTGGAACTGCAGGGGCTGGGGGCTGGGGGCTAGGGGCCAGGGGCTAGGGGCTAGGGGCTAGGGGCTAGGGGC
TAGGGGTTGGGAACAGCGAA

Product: pyranopterin triphosphate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MALIDAYGRRINYLRLSVTDRCNMRCSYCMPAQGVEKLEHKEMLSYEELYRVAGACIAQGIEKIRVTGGEPLVRKGIVPF
LERLARVPGLKELVLTTNGLQLEEMAQPLKRAGVARLNISLDSLRPEVFARITRGADLKRVLSGIEAAEKAGFANLKINM
VVMRGVNDDEILDFAALSIEKPYTVRFIEYMPTLQDEGWNAQSMPGSEILARIAERYPLLPMVGAEMAGPARNFKIEGAA
GAIGIITPVSGHFCESCNRIRVTAAGRMRGCLFSDQGVDLKPLLQSLDPYRLQEEIRRIVTHKPGRHHIAEPEAEKITVN
MSRIGG

Sequences:

>Translated_326_residues
MALIDAYGRRINYLRLSVTDRCNMRCSYCMPAQGVEKLEHKEMLSYEELYRVAGACIAQGIEKIRVTGGEPLVRKGIVPF
LERLARVPGLKELVLTTNGLQLEEMAQPLKRAGVARLNISLDSLRPEVFARITRGADLKRVLSGIEAAEKAGFANLKINM
VVMRGVNDDEILDFAALSIEKPYTVRFIEYMPTLQDEGWNAQSMPGSEILARIAERYPLLPMVGAEMAGPARNFKIEGAA
GAIGIITPVSGHFCESCNRIRVTAAGRMRGCLFSDQGVDLKPLLQSLDPYRLQEEIRRIVTHKPGRHHIAEPEAEKITVN
MSRIGG
>Mature_325_residues
ALIDAYGRRINYLRLSVTDRCNMRCSYCMPAQGVEKLEHKEMLSYEELYRVAGACIAQGIEKIRVTGGEPLVRKGIVPFL
ERLARVPGLKELVLTTNGLQLEEMAQPLKRAGVARLNISLDSLRPEVFARITRGADLKRVLSGIEAAEKAGFANLKINMV
VMRGVNDDEILDFAALSIEKPYTVRFIEYMPTLQDEGWNAQSMPGSEILARIAERYPLLPMVGAEMAGPARNFKIEGAAG
AIGIITPVSGHFCESCNRIRVTAAGRMRGCLFSDQGVDLKPLLQSLDPYRLQEEIRRIVTHKPGRHHIAEPEAEKITVNM
SRIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI116734668, Length=305, Percent_Identity=41.9672131147541, Blast_Score=248, Evalue=4e-66,
Organism=Homo sapiens, GI22027536, Length=305, Percent_Identity=41.9672131147541, Blast_Score=248, Evalue=5e-66,
Organism=Escherichia coli, GI1786999, Length=325, Percent_Identity=33.8461538461538, Blast_Score=195, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI32566331, Length=309, Percent_Identity=37.8640776699029, Blast_Score=226, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI32566329, Length=307, Percent_Identity=38.1107491856677, Blast_Score=224, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI17561210, Length=309, Percent_Identity=37.8640776699029, Blast_Score=223, Evalue=8e-59,
Organism=Drosophila melanogaster, GI28574490, Length=313, Percent_Identity=38.9776357827476, Blast_Score=226, Evalue=1e-59,
Organism=Drosophila melanogaster, GI28574488, Length=307, Percent_Identity=38.7622149837134, Blast_Score=223, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 36162; Mature: 36031

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIDAYGRRINYLRLSVTDRCNMRCSYCMPAQGVEKLEHKEMLSYEELYRVAGACIAQG
CCCHHHHCCEEEEEEEEECCCCCCEECCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
IEKIRVTGGEPLVRKGIVPFLERLARVPGLKELVLTTNGLQLEEMAQPLKRAGVARLNIS
HHHEEECCCCHHHHHCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEE
LDSLRPEVFARITRGADLKRVLSGIEAAEKAGFANLKINMVVMRGVNDDEILDFAALSIE
HHHHCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHCC
KPYTVRFIEYMPTLQDEGWNAQSMPGSEILARIAERYPLLPMVGAEMAGPARNFKIEGAA
CCCEEEHEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHCCCCCCEEECCCC
GAIGIITPVSGHFCESCNRIRVTAAGRMRGCLFSDQGVDLKPLLQSLDPYRLQEEIRRIV
CCEEEECCCCCHHHHCCCEEEEEECCCHHCCEECCCCCCHHHHHHCCCHHHHHHHHHHHH
THKPGRHHIAEPEAEKITVNMSRIGG
CCCCCCCCCCCCCCCEEEEEHHHCCC
>Mature Secondary Structure 
ALIDAYGRRINYLRLSVTDRCNMRCSYCMPAQGVEKLEHKEMLSYEELYRVAGACIAQG
CCHHHHCCEEEEEEEEECCCCCCEECCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
IEKIRVTGGEPLVRKGIVPFLERLARVPGLKELVLTTNGLQLEEMAQPLKRAGVARLNIS
HHHEEECCCCHHHHHCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEE
LDSLRPEVFARITRGADLKRVLSGIEAAEKAGFANLKINMVVMRGVNDDEILDFAALSIE
HHHHCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHCC
KPYTVRFIEYMPTLQDEGWNAQSMPGSEILARIAERYPLLPMVGAEMAGPARNFKIEGAA
CCCEEEHEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHCCCCCCEEECCCC
GAIGIITPVSGHFCESCNRIRVTAAGRMRGCLFSDQGVDLKPLLQSLDPYRLQEEIRRIV
CCEEEECCCCCHHHHCCCEEEEEECCCHHCCEECCCCCCHHHHHHCCCHHHHHHHHHHHH
THKPGRHHIAEPEAEKITVNMSRIGG
CCCCCCCCCCCCCCCEEEEEHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA