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The map label for this gene is pleD [H]

Identifier: 197117720

GI number: 197117720

Start: 1535717

End: 1536637

Strand: Direct

Name: pleD [H]

Synonym: Gbem_1332

Alternate gene names: 197117720

Gene position: 1535717-1536637 (Clockwise)

Preceding gene: 308535230

Following gene: 197117721

Centisome position: 33.28

GC content: 59.5

Gene sequence:

>921_bases
ATGTCCAGCTGTGTGCTCGTGATCGATGACTCCGCTGCCATCCGGGAGCAGGTTGTGCGGACGCTCAAGGATGTCGGACT
GTTCGAGGAATACTGCGAGGCGCGGGACGGGCTCGAAGGCTTCAAGACGCTGATCGAGTCGAAGGCCGATCTGGTGATCT
GCGACGTCGACATGCCGCGCATGGACGGCTACAAGTTCCTGCAGCTCGTCGCGTCGAGACCCGAGTTGCTGGGGCTTCCG
ATCATCATGCTGACCGGCATGATGGACTTCAACTCAAAAATTAGGGGGCTGGAGCAGGGGGCGAGCGATTATCTCACCAA
GCCGTTCGATTCCGGCGAGCTTGTGGCACGGGTCAAGGTACAGCTGAAGATCAAGTCGCTGCAGGACGAGCTGAAAAAGG
CGAACGAACAATTAAAGAGACTCACCAACATCGATCACCTGACCAACCTCTTCAACCGGCGCTACCTCACAGAGGTACTC
GAATCGGAATTCTTCCGCGCCCGGCGCAACCGGGAGAACCTCTCGCTGGTCATCATCGACATAGACTATTTCAAGAACGT
GAACGACACCTACGGCCATCAAAACGGTGACGTGGTGCTTGCTTCCGTCGCCGGGCTGGCACAGAAGCAGTTGCGCGCCT
ACGACAGCGCCGCCCGCTATGGCGGCGAAGAGTTCGTCCTGGTCCTGCCAGGGACTTCGCTGGAAGGAGGGAAAATGGTG
GCCGAGCGCCTGAGGCAGGCGGTGCTGGAATTCGCTTTCCCGTCCCCCATGGAAGATCTCACCCTGACCATCAGCGCCGG
CGTGGCGACCTTTCCCTCGCCCAGTATCGATAACATCGACTCCTTGTTCCGCCAGGCCGACGAGGCGCTGTACCGGGCCA
AGCAAACCGGCCGGAACAGGGTAGAGCTCATGGACGTCTGA

Upstream 100 bases:

>100_bases
AGCATGATGCTATTTGGCACAGCAAGCCAGGCATGAAGATGGGGCGCTGTACCTGTCGTTCAATCGGTTAAGAATTATTT
GAGGCTATTGGGGAGATTTC

Downstream 100 bases:

>100_bases
TTCATCCGGCAGTCAATCCCGGCATGCGGCCCCCCCTCCGGCGGCGTTACCCTTTCCCTTAGATATCCCACACGGCATTC
CTTATTAAAATCCTTCGTCA

Product: response receiver-modulated diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MSSCVLVIDDSAAIREQVVRTLKDVGLFEEYCEARDGLEGFKTLIESKADLVICDVDMPRMDGYKFLQLVASRPELLGLP
IIMLTGMMDFNSKIRGLEQGASDYLTKPFDSGELVARVKVQLKIKSLQDELKKANEQLKRLTNIDHLTNLFNRRYLTEVL
ESEFFRARRNRENLSLVIIDIDYFKNVNDTYGHQNGDVVLASVAGLAQKQLRAYDSAARYGGEEFVLVLPGTSLEGGKMV
AERLRQAVLEFAFPSPMEDLTLTISAGVATFPSPSIDNIDSLFRQADEALYRAKQTGRNRVELMDV

Sequences:

>Translated_306_residues
MSSCVLVIDDSAAIREQVVRTLKDVGLFEEYCEARDGLEGFKTLIESKADLVICDVDMPRMDGYKFLQLVASRPELLGLP
IIMLTGMMDFNSKIRGLEQGASDYLTKPFDSGELVARVKVQLKIKSLQDELKKANEQLKRLTNIDHLTNLFNRRYLTEVL
ESEFFRARRNRENLSLVIIDIDYFKNVNDTYGHQNGDVVLASVAGLAQKQLRAYDSAARYGGEEFVLVLPGTSLEGGKMV
AERLRQAVLEFAFPSPMEDLTLTISAGVATFPSPSIDNIDSLFRQADEALYRAKQTGRNRVELMDV
>Mature_305_residues
SSCVLVIDDSAAIREQVVRTLKDVGLFEEYCEARDGLEGFKTLIESKADLVICDVDMPRMDGYKFLQLVASRPELLGLPI
IMLTGMMDFNSKIRGLEQGASDYLTKPFDSGELVARVKVQLKIKSLQDELKKANEQLKRLTNIDHLTNLFNRRYLTEVLE
SEFFRARRNRENLSLVIIDIDYFKNVNDTYGHQNGDVVLASVAGLAQKQLRAYDSAARYGGEEFVLVLPGTSLEGGKMVA
ERLRQAVLEFAFPSPMEDLTLTISAGVATFPSPSIDNIDSLFRQADEALYRAKQTGRNRVELMDV

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786584, Length=174, Percent_Identity=35.0574712643678, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI145693134, Length=194, Percent_Identity=38.659793814433, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI87081881, Length=173, Percent_Identity=39.8843930635838, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1787262, Length=175, Percent_Identity=39.4285714285714, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1787816, Length=167, Percent_Identity=40.1197604790419, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1787802, Length=193, Percent_Identity=32.6424870466321, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87082007, Length=161, Percent_Identity=41.6149068322981, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788381, Length=177, Percent_Identity=35.0282485875706, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1788085, Length=164, Percent_Identity=34.1463414634146, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787541, Length=171, Percent_Identity=32.1637426900585, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1786599, Length=123, Percent_Identity=35.7723577235772, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082012, Length=120, Percent_Identity=36.6666666666667, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081974, Length=156, Percent_Identity=28.8461538461538, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790299, Length=134, Percent_Identity=35.0746268656716, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786784, Length=119, Percent_Identity=31.0924369747899, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1788550, Length=146, Percent_Identity=30.1369863013699, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788191, Length=109, Percent_Identity=33.9449541284404, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786911, Length=124, Percent_Identity=33.8709677419355, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87081977, Length=182, Percent_Identity=27.4725274725275, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787056, Length=128, Percent_Identity=32.8125, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787375, Length=122, Percent_Identity=31.9672131147541, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 34374; Mature: 34243

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSCVLVIDDSAAIREQVVRTLKDVGLFEEYCEARDGLEGFKTLIESKADLVICDVDMPR
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCC
MDGYKFLQLVASRPELLGLPIIMLTGMMDFNSKIRGLEQGASDYLTKPFDSGELVARVKV
CCHHHHHHHHHCCCHHHCCHHHHHHCCHHHHHHHHHHHCCCHHHHCCCCCCCCEEEEEEH
QLKIKSLQDELKKANEQLKRLTNIDHLTNLFNRRYLTEVLESEFFRARRNRENLSLVIID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
IDYFKNVNDTYGHQNGDVVLASVAGLAQKQLRAYDSAARYGGEEFVLVLPGTSLEGGKMV
EHHHCCCCHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHH
AERLRQAVLEFAFPSPMEDLTLTISAGVATFPSPSIDNIDSLFRQADEALYRAKQTGRNR
HHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
VELMDV
EEEECC
>Mature Secondary Structure 
SSCVLVIDDSAAIREQVVRTLKDVGLFEEYCEARDGLEGFKTLIESKADLVICDVDMPR
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCC
MDGYKFLQLVASRPELLGLPIIMLTGMMDFNSKIRGLEQGASDYLTKPFDSGELVARVKV
CCHHHHHHHHHCCCHHHCCHHHHHHCCHHHHHHHHHHHCCCHHHHCCCCCCCCEEEEEEH
QLKIKSLQDELKKANEQLKRLTNIDHLTNLFNRRYLTEVLESEFFRARRNRENLSLVIID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
IDYFKNVNDTYGHQNGDVVLASVAGLAQKQLRAYDSAARYGGEEFVLVLPGTSLEGGKMV
EHHHCCCCHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHH
AERLRQAVLEFAFPSPMEDLTLTISAGVATFPSPSIDNIDSLFRQADEALYRAKQTGRNR
HHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
VELMDV
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA