Click here to switch to the map view.

The map label for this gene is aphA [H]

Identifier: 197117530

GI number: 197117530

Start: 1318771

End: 1320333

Strand: Reverse

Name: aphA [H]

Synonym: Gbem_1141

Alternate gene names: 197117530

Gene position: 1320333-1318771 (Counterclockwise)

Preceding gene: 197117531

Following gene: 197117527

Centisome position: 28.61

GC content: 60.84

Gene sequence:

>1563_bases
ATGACCGATAAAGATCTCACTACCGATTCAGGCGCGCGCAAGCAGGAGCCCGGGCAACTGCAGGGGGACCTACCCTTTCG
GCTGCTGGTGGAGCAGGTGAAGGAGTACGCAGTGGCGCTTTTGGATCCGCAGGGAAACATCTCCTCGTGGAACGCCGGGG
CCGAGCAGATTACCAAGTATCGGGGCAGCGAGGTGATCGGGAAGCATTTCTCGCTGCTCCACCCCAAGGAGGAACTGCGT
TCGGGGGCGCCGGAAAGGGAGCTCGCCATTGCACGCTCGCTGGGGAGCCTGGACCTGCAGGGGTGGCGACTGAAAAAAGA
CGGTAGCCGCTTCTGGGCCGCAATCACGCTCACCGCGCTCTACGATCAGGGAGAAAACCTCGCCGGGTTTGCGCTGATAG
CACACGACAACACGGAGAAACGCGCTTCTGACGAGGCGCTGGTGAAAAGCCGCAACATGCTGGAGCGGCTCTTCGAAACG
GCTCCGGACGGCATCGTAGTGGTCGACGACAACGGTGTCATTCGCAGGACGAACCAGCAGGCGGAGATCACCTTCGGCTA
CATGCGTGAGGAGATGCTGGGGCAGCGCATCGAGCTCTTGGTCCCGGAGCGTTATCACAAGCACCATCGCCAACACCGGC
GCAACTATTTCGCCGACCCGCGCGCCCGCAAGATGGGGATCGGCCTCGAACTGTACGGCCGCCACAAGGACGGTCACGAG
ATCCCGGTAGACATCATGCTAAACCCCATCGAGACGCCGGACGGGACCTGGGTCTTCGCCGTGATCCGCGACATAACCAG
CCAAAGGCAAGGTGAGGCGAAGATACTGGAGCTGAACCTTGCCCTGAGAAACCAGCTCGAGCAGTTGGGGGCAAGCAACA
AGGAGCTGGAATCGTTCAGCTACTCGGTTTCCCACGACCTGCGGGCCCCGCTGCGCCACATCATCGGGTTCGTGGACCTG
TTGAACGCCAAGGCCGGGAACGTTCTGGACGAGAAGAGCCGCCACTATCTGGAGGTAATCAGCGAGGCGGCCAACAAGAT
GGGGTTGCTGATCGACGACCTGCTGGCCTTCTCGCGCATGGGGCGCAGCGAAATGATGAAGGGGTGGGTAGACCTGGGAC
TGCTGGTGAGAGAGATAGTGAACGACCTGGAAAGCGATAGCAAAGAGAGGGAGATCCAATGGGACATAGCCCCGCTCCCC
ATCGTGCTTGGGGATGCAGCAATGCTGCGCCAGGTTCTCATCAACCTCATCGGCAACGCGGTCAAGTTCACCCGGTCGCG
GGAAGAAGCAAAAATCGCCATTGGCGCCATCGACCAGGAGCAGGAAACGGAGATTTTTGTAAGGGACAACGGGGTCGGGT
TCGACGAGGCTTATGCGAGCAAGCTTTTCGGCCTTTTCCAGCGTCTGCATGCCAATGAGGAGTTCGAGGGAACCGGGGTC
GGGCTGGCTATCGTGCAGCGGATCGTACTACGACACGGCGGCAGGGTATGGGCTGAGGGCGCGGTCGATGGCGGAGCAAC
CTTCCGGTTCTCGCTCCCGAAGGGACTAAACCCGGCGCCCTAG

Upstream 100 bases:

>100_bases
AGCGACGGCGGAGACGGATTGCAGCGCGACGAGACGCCATGGATGCAGAGCTTCGAGCGGGTAGTAGTAGCTCCACGTTG
ACGCGGAACGGGGAAAAACC

Downstream 100 bases:

>100_bases
AAGAAGCAGCTAATTACCTGCGCCCGCCGGGCTCGCCTTTAGCTTTAGAGCTTGTTCCTCAGGGTTTCCTTGAGTTCCAA
AATGGCCAGTGTCTGCATGT

Product: PAS/PAS domain-containing sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MTDKDLTTDSGARKQEPGQLQGDLPFRLLVEQVKEYAVALLDPQGNISSWNAGAEQITKYRGSEVIGKHFSLLHPKEELR
SGAPERELAIARSLGSLDLQGWRLKKDGSRFWAAITLTALYDQGENLAGFALIAHDNTEKRASDEALVKSRNMLERLFET
APDGIVVVDDNGVIRRTNQQAEITFGYMREEMLGQRIELLVPERYHKHHRQHRRNYFADPRARKMGIGLELYGRHKDGHE
IPVDIMLNPIETPDGTWVFAVIRDITSQRQGEAKILELNLALRNQLEQLGASNKELESFSYSVSHDLRAPLRHIIGFVDL
LNAKAGNVLDEKSRHYLEVISEAANKMGLLIDDLLAFSRMGRSEMMKGWVDLGLLVREIVNDLESDSKEREIQWDIAPLP
IVLGDAAMLRQVLINLIGNAVKFTRSREEAKIAIGAIDQEQETEIFVRDNGVGFDEAYASKLFGLFQRLHANEEFEGTGV
GLAIVQRIVLRHGGRVWAEGAVDGGATFRFSLPKGLNPAP

Sequences:

>Translated_520_residues
MTDKDLTTDSGARKQEPGQLQGDLPFRLLVEQVKEYAVALLDPQGNISSWNAGAEQITKYRGSEVIGKHFSLLHPKEELR
SGAPERELAIARSLGSLDLQGWRLKKDGSRFWAAITLTALYDQGENLAGFALIAHDNTEKRASDEALVKSRNMLERLFET
APDGIVVVDDNGVIRRTNQQAEITFGYMREEMLGQRIELLVPERYHKHHRQHRRNYFADPRARKMGIGLELYGRHKDGHE
IPVDIMLNPIETPDGTWVFAVIRDITSQRQGEAKILELNLALRNQLEQLGASNKELESFSYSVSHDLRAPLRHIIGFVDL
LNAKAGNVLDEKSRHYLEVISEAANKMGLLIDDLLAFSRMGRSEMMKGWVDLGLLVREIVNDLESDSKEREIQWDIAPLP
IVLGDAAMLRQVLINLIGNAVKFTRSREEAKIAIGAIDQEQETEIFVRDNGVGFDEAYASKLFGLFQRLHANEEFEGTGV
GLAIVQRIVLRHGGRVWAEGAVDGGATFRFSLPKGLNPAP
>Mature_519_residues
TDKDLTTDSGARKQEPGQLQGDLPFRLLVEQVKEYAVALLDPQGNISSWNAGAEQITKYRGSEVIGKHFSLLHPKEELRS
GAPERELAIARSLGSLDLQGWRLKKDGSRFWAAITLTALYDQGENLAGFALIAHDNTEKRASDEALVKSRNMLERLFETA
PDGIVVVDDNGVIRRTNQQAEITFGYMREEMLGQRIELLVPERYHKHHRQHRRNYFADPRARKMGIGLELYGRHKDGHEI
PVDIMLNPIETPDGTWVFAVIRDITSQRQGEAKILELNLALRNQLEQLGASNKELESFSYSVSHDLRAPLRHIIGFVDLL
NAKAGNVLDEKSRHYLEVISEAANKMGLLIDDLLAFSRMGRSEMMKGWVDLGLLVREIVNDLESDSKEREIQWDIAPLPI
VLGDAAMLRQVLINLIGNAVKFTRSREEAKIAIGAIDQEQETEIFVRDNGVGFDEAYASKLFGLFQRLHANEEFEGTGVG
LAIVQRIVLRHGGRVWAEGAVDGGATFRFSLPKGLNPAP

Specific function: Photoreceptor which exists in two forms that are reversibly interconvertible by light:the R form that absorbs maximally in the red region of the spectrum and the FR form that absorbs maximally in the far-red region [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=387, Percent_Identity=26.0981912144703, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1789149, Length=264, Percent_Identity=30.3030303030303, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1786912, Length=220, Percent_Identity=30.9090909090909, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI48994928, Length=234, Percent_Identity=26.4957264957265, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1786600, Length=436, Percent_Identity=24.7706422018349, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI145693157, Length=265, Percent_Identity=27.9245283018868, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788713, Length=227, Percent_Identity=28.1938325991189, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790861, Length=238, Percent_Identity=28.1512605042017, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1790436, Length=223, Percent_Identity=28.2511210762332, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI87081816, Length=231, Percent_Identity=27.2727272727273, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786783, Length=242, Percent_Identity=23.5537190082645, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790346, Length=235, Percent_Identity=28.936170212766, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1787894, Length=244, Percent_Identity=26.2295081967213, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1789808, Length=212, Percent_Identity=27.8301886792453, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1788393, Length=240, Percent_Identity=27.5, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003018
- InterPro:   IPR013654
- InterPro:   IPR016132
- InterPro:   IPR001294
- InterPro:   IPR013515
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF01590 GAF; PF02518 HATPase_c; PF00512 HisKA; PF08446 PAS_2; PF00360 Phytochrome [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 58312; Mature: 58181

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKDLTTDSGARKQEPGQLQGDLPFRLLVEQVKEYAVALLDPQGNISSWNAGAEQITKY
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHH
RGSEVIGKHFSLLHPKEELRSGAPERELAIARSLGSLDLQGWRLKKDGSRFWAAITLTAL
CCCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHCCCCCCCCCEECCCCCCEEHHEEEEHH
YDQGENLAGFALIAHDNTEKRASDEALVKSRNMLERLFETAPDGIVVVDDNGVIRRTNQQ
HCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEECCCC
AEITFGYMREEMLGQRIELLVPERYHKHHRQHRRNYFADPRARKMGIGLELYGRHKDGHE
CEEHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEECCCCCCC
IPVDIMLNPIETPDGTWVFAVIRDITSQRQGEAKILELNLALRNQLEQLGASNKELESFS
CCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCCCCCHHHHHH
YSVSHDLRAPLRHIIGFVDLLNAKAGNVLDEKSRHYLEVISEAANKMGLLIDDLLAFSRM
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRSEMMKGWVDLGLLVREIVNDLESDSKEREIQWDIAPLPIVLGDAAMLRQVLINLIGNA
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEECCHHHHHHHHHHHHHHH
VKFTRSREEAKIAIGAIDQEQETEIFVRDNGVGFDEAYASKLFGLFQRLHANEEFEGTGV
HHHHCCCCCCEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCH
GLAIVQRIVLRHGGRVWAEGAVDGGATFRFSLPKGLNPAP
HHHHHHHHHHHCCCCEEECCCCCCCCEEEEECCCCCCCCC
>Mature Secondary Structure 
TDKDLTTDSGARKQEPGQLQGDLPFRLLVEQVKEYAVALLDPQGNISSWNAGAEQITKY
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHH
RGSEVIGKHFSLLHPKEELRSGAPERELAIARSLGSLDLQGWRLKKDGSRFWAAITLTAL
CCCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHCCCCCCCCCEECCCCCCEEHHEEEEHH
YDQGENLAGFALIAHDNTEKRASDEALVKSRNMLERLFETAPDGIVVVDDNGVIRRTNQQ
HCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEECCCC
AEITFGYMREEMLGQRIELLVPERYHKHHRQHRRNYFADPRARKMGIGLELYGRHKDGHE
CEEHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEECCCCCCC
IPVDIMLNPIETPDGTWVFAVIRDITSQRQGEAKILELNLALRNQLEQLGASNKELESFS
CCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCCCCCHHHHHH
YSVSHDLRAPLRHIIGFVDLLNAKAGNVLDEKSRHYLEVISEAANKMGLLIDDLLAFSRM
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRSEMMKGWVDLGLLVREIVNDLESDSKEREIQWDIAPLPIVLGDAAMLRQVLINLIGNA
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEECCHHHHHHHHHHHHHHH
VKFTRSREEAKIAIGAIDQEQETEIFVRDNGVGFDEAYASKLFGLFQRLHANEEFEGTGV
HHHHCCCCCCEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCH
GLAIVQRIVLRHGGRVWAEGAVDGGATFRFSLPKGLNPAP
HHHHHHHHHHHCCCCEEECCCCCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]