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The map label for this gene is zraS [H]

Identifier: 197117483

GI number: 197117483

Start: 1266700

End: 1267794

Strand: Direct

Name: zraS [H]

Synonym: Gbem_1094

Alternate gene names: 197117483

Gene position: 1266700-1267794 (Clockwise)

Preceding gene: 197117482

Following gene: 197117484

Centisome position: 27.45

GC content: 62.65

Gene sequence:

>1095_bases
ATGAAGAGAACTGACCTTGCCCGAGGTACGGTGCTCGTTGCCTGCGTCATAGGGATCAGCCTTTTGCATTACCTCACCCC
GCTGCGGCTTGCCATGCTGCACGACATCTTCCAGCGCCTCTACTACATTCCCATCATCCTCGCCGCCTACTGGTTCGGCT
TTCGCGGCGGCATCGGCTGCGCCCTGCTGGTCACCTTCTGTTACGCCCCGCACATCCTGTTCCAGTGGGGGGGGCACATC
GCCATGGAAATGGAGAAGTACCTCGAGGTGATCCTCTACAACGTGGTAGGGGGGATCACCGGGATGCTCTCGCAGCAGGA
ACGGTCCCGCAGGGAGCAGCTTGAGGAGACGGCGAAGGGGCTGGAAGATTCCTACCGGCAGTTGAGGACCCAGTCCGAAC
TCATCATCAGGATTGAGGAGCAGTTGCGCCGGGCGGAGCGCCTGTCGGCTCTTGGTGAGCTTTCCGCCATCCTGGCCCAC
GAGATACGCAACCCGCTGGCCTCGATACGCGGTACCGCCGAGATCCTCAGGGAGGATGGGGCGGCCGCCAACCGCGACGA
GTTCCTGGGGATACTGGTGAAGGAATCTGACCGCCTGAACCACGTGGTGGAGGATTTCCTGCGCATGGCGCGCTCCGAGC
CGGCGATCAAGAAAGAGTGCGACATCAACTACGAGCTTGCCAACATGGTAACGCTCCTCTCTGCCCAGGCCCGAGGCGAG
GAGGTGGAACTGCGGTTTACTCCCGGGGAGGTTCCGACGCTTTCCGCCGATCCGGAAAAGCTGCGCCAAGCATTCATGAA
CGTCATCCTCAACGGGATCCAGGCTTCCGCTCCAGGGGGGCGGGTCCTGGTGACGACGAGCTACGACCGCCAAAGTCGCA
GCATCGAGATCCGCTTCAGCGACAACGGCCCCGGCATCGCGCGCGAGGCGATCGGCAAGATCTTCGAACCCTTCTTCACC
ACCAAGGGAAGCGGCACCGGCCTCGGGCTCTCCATCACCAAAAAGATACTGGAGGGACACGGCGGGACGCTGGAAGTGGA
AAGCACGCCGGGCGAGGGGGCCTGCTTCCGGGCCCGGCTGCCGACGGAAACCTAA

Upstream 100 bases:

>100_bases
GCTACGACCCGGAAGGAGCGGATACCGAGACCATCCAGTCGCAGGCCGTGGAGCTCTGCCCGGTCTCCTGCATCGTGTGG
GTGGAATGACGGGGAGCGCC

Downstream 100 bases:

>100_bases
GTGGCGGGCTGTGGAAAATTCTACGGGGTTGTTGGATATTCTACAGGCTGCGCCGTCAGCTGCCGACGAGGCCGCTAGCG
CCGCTAATGATACCTTGCAG

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MKRTDLARGTVLVACVIGISLLHYLTPLRLAMLHDIFQRLYYIPIILAAYWFGFRGGIGCALLVTFCYAPHILFQWGGHI
AMEMEKYLEVILYNVVGGITGMLSQQERSRREQLEETAKGLEDSYRQLRTQSELIIRIEEQLRRAERLSALGELSAILAH
EIRNPLASIRGTAEILREDGAAANRDEFLGILVKESDRLNHVVEDFLRMARSEPAIKKECDINYELANMVTLLSAQARGE
EVELRFTPGEVPTLSADPEKLRQAFMNVILNGIQASAPGGRVLVTTSYDRQSRSIEIRFSDNGPGIAREAIGKIFEPFFT
TKGSGTGLGLSITKKILEGHGGTLEVESTPGEGACFRARLPTET

Sequences:

>Translated_364_residues
MKRTDLARGTVLVACVIGISLLHYLTPLRLAMLHDIFQRLYYIPIILAAYWFGFRGGIGCALLVTFCYAPHILFQWGGHI
AMEMEKYLEVILYNVVGGITGMLSQQERSRREQLEETAKGLEDSYRQLRTQSELIIRIEEQLRRAERLSALGELSAILAH
EIRNPLASIRGTAEILREDGAAANRDEFLGILVKESDRLNHVVEDFLRMARSEPAIKKECDINYELANMVTLLSAQARGE
EVELRFTPGEVPTLSADPEKLRQAFMNVILNGIQASAPGGRVLVTTSYDRQSRSIEIRFSDNGPGIAREAIGKIFEPFFT
TKGSGTGLGLSITKKILEGHGGTLEVESTPGEGACFRARLPTET
>Mature_364_residues
MKRTDLARGTVLVACVIGISLLHYLTPLRLAMLHDIFQRLYYIPIILAAYWFGFRGGIGCALLVTFCYAPHILFQWGGHI
AMEMEKYLEVILYNVVGGITGMLSQQERSRREQLEETAKGLEDSYRQLRTQSELIIRIEEQLRRAERLSALGELSAILAH
EIRNPLASIRGTAEILREDGAAANRDEFLGILVKESDRLNHVVEDFLRMARSEPAIKKECDINYELANMVTLLSAQARGE
EVELRFTPGEVPTLSADPEKLRQAFMNVILNGIQASAPGGRVLVTTSYDRQSRSIEIRFSDNGPGIAREAIGKIFEPFFT
TKGSGTGLGLSITKKILEGHGGTLEVESTPGEGACFRARLPTET

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=228, Percent_Identity=39.4736842105263, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1788549, Length=238, Percent_Identity=39.0756302521008, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI1790300, Length=232, Percent_Identity=30.6034482758621, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI87081816, Length=239, Percent_Identity=32.2175732217573, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788393, Length=242, Percent_Identity=27.2727272727273, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786600, Length=216, Percent_Identity=26.3888888888889, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI145693157, Length=306, Percent_Identity=24.1830065359477, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789808, Length=215, Percent_Identity=29.3023255813954, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI48994928, Length=236, Percent_Identity=27.9661016949153, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790861, Length=210, Percent_Identity=28.0952380952381, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786783, Length=242, Percent_Identity=28.099173553719, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1790346, Length=242, Percent_Identity=28.5123966942149, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87082128, Length=248, Percent_Identity=28.2258064516129, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1789149, Length=280, Percent_Identity=23.2142857142857, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1786912, Length=239, Percent_Identity=28.4518828451883, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788713, Length=254, Percent_Identity=25.5905511811024, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1790567, Length=99, Percent_Identity=38.3838383838384, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 40482; Mature: 40482

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTDLARGTVLVACVIGISLLHYLTPLRLAMLHDIFQRLYYIPIILAAYWFGFRGGIGC
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
ALLVTFCYAPHILFQWGGHIAMEMEKYLEVILYNVVGGITGMLSQQERSRREQLEETAKG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEDSYRQLRTQSELIIRIEEQLRRAERLSALGELSAILAHEIRNPLASIRGTAEILREDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AAANRDEFLGILVKESDRLNHVVEDFLRMARSEPAIKKECDINYELANMVTLLSAQARGE
CCCCCHHHEEEEEECCHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHCCCC
EVELRFTPGEVPTLSADPEKLRQAFMNVILNGIQASAPGGRVLVTTSYDRQSRSIEIRFS
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEEC
DNGPGIAREAIGKIFEPFFTTKGSGTGLGLSITKKILEGHGGTLEVESTPGEGACFRARL
CCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCEEECCC
PTET
CCCC
>Mature Secondary Structure
MKRTDLARGTVLVACVIGISLLHYLTPLRLAMLHDIFQRLYYIPIILAAYWFGFRGGIGC
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
ALLVTFCYAPHILFQWGGHIAMEMEKYLEVILYNVVGGITGMLSQQERSRREQLEETAKG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEDSYRQLRTQSELIIRIEEQLRRAERLSALGELSAILAHEIRNPLASIRGTAEILREDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AAANRDEFLGILVKESDRLNHVVEDFLRMARSEPAIKKECDINYELANMVTLLSAQARGE
CCCCCHHHEEEEEECCHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHCCCC
EVELRFTPGEVPTLSADPEKLRQAFMNVILNGIQASAPGGRVLVTTSYDRQSRSIEIRFS
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEEC
DNGPGIAREAIGKIFEPFFTTKGSGTGLGLSITKKILEGHGGTLEVESTPGEGACFRARL
CCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCEEECCC
PTET
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]